(Circular) (Six-base) MAPSORT of: pFN21AE0614.seq Check: 193 from: 1 to: 5797 pFN21AE0614 5797 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2795 3860 5244 5512 1632 Size: 1163 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1163 1065 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 2847 4696 5673 1203 Size: 1644 1849 977 1327 Fragments arranged by size: 1849 1644 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 2885 2906 3106 3332 3484 5593 1493 Size: 1392 21 200 226 152 2109 1697 Fragments arranged by size: 2109 1697 1392 226 200 152 21 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 BamHI G'GATC_C Cuts at: 3127 3494 3127 Size: 367 5430 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2599 3118 4573 618 Size: 325 217 627 812 519 1455 1842 Fragments arranged by size: 1842 1455 812 627 519 325 217 BanII G_rGCy'C Cuts at: 1391 3116 1391 Size: 1725 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2620 2762 2783 928 Size: 971 721 142 21 3942 Fragments arranged by size: 3942 971 721 142 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2075 2109 3988 4022 1903 Size: 34 138 34 1879 34 3678 Fragments arranged by size: 3678 1879 138 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2749 3689 4899 2749 Size: 940 1210 3647 Fragments arranged by size: 3647 1210 940 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 3858 5014 1790 Size: 2068 1156 2573 Fragments arranged by size: 2573 2068 1156 BmgBI CAC'GTC Cuts at: 1318 2848 2869 1318 Size: 1530 21 4246 Fragments arranged by size: 4246 1530 21 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 2544 3528 Size: 416 53 83 399 24 624 503 984 Cuts at: 3528 4486 442 Size: 958 1753 Fragments arranged by size: 1753 984 958 624 503 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2739 2739 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3087 4457 1239 Size: 1848 1370 2579 Fragments arranged by size: 2579 1848 1370 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3922 4787 5085 3922 Size: 865 298 4634 Fragments arranged by size: 4634 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4460 882 Size: 617 2961 2219 Fragments arranged by size: 2961 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3604 3986 4621 275 Size: 53 83 186 936 2071 382 635 1451 Fragments arranged by size: 2071 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5572 Fragments arranged by size: 5572 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5797 BseYI C'CCAG_C Cuts at: 2297 5000 5496 2297 Size: 2703 496 2598 Fragments arranged by size: 2703 2598 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3858 3943 5014 3858 Size: 85 1071 4641 Fragments arranged by size: 4641 1071 85 BsmI GAATG_Cn' Cuts at: 3308 3401 3308 Size: 93 5704 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3116 3858 3943 5014 1391 Size: 399 1326 742 85 1071 2174 Fragments arranged by size: 2174 1326 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3152 844 Size: 2308 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3033 3684 1394 Size: 429 1210 651 3507 Fragments arranged by size: 3507 1210 651 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2091 3085 4292 4466 65 Size: 1183 843 994 1207 174 1396 Fragments arranged by size: 1396 1207 1183 994 843 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2487 3008 4441 4628 1700 Size: 110 677 521 1433 187 2869 Fragments arranged by size: 2869 1433 677 521 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 2650 1184 Size: 1466 4331 BstXI CCAn_nnnn'nTGG Cuts at: 2209 2422 2536 2209 Size: 213 114 5470 Fragments arranged by size: 5470 213 114 BstYI r'GATC_y Cuts at: 1972 2976 3127 3494 3879 3896 5348 5434 5446 Size: 1004 151 367 385 17 1452 86 12 Cuts at: 5446 5792 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1004 385 367 346 151 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4306 Fragments arranged by size: 4306 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 3001 3313 4107 4127 3001 Size: 312 794 20 4671 Fragments arranged by size: 4671 794 312 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DraIII CAC_nnn'GTG Cuts at: 3091 3091 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2412 2526 Size: 54 1425 50 275 18 369 213 114 Cuts at: 2526 3244 4133 5762 8 Size: 718 889 1629 43 Fragments arranged by size: 1629 1425 889 718 369 275 213 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3727 1353 Size: 2374 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2761 4384 4758 4904 387 Size: 1064 138 1172 1623 374 146 1280 Fragments arranged by size: 1623 1280 1172 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2795 3087 3860 4457 5244 5512 1239 Size: 393 1163 292 773 597 787 268 1524 Fragments arranged by size: 1524 1163 787 773 597 393 292 268 EcoNI CCTnn'n_nnAGG Cuts at: 2375 2375 Size: 5797 EcoO109I rG'GnC_Cy Cuts at: 2333 2380 2799 2333 Size: 47 419 5331 Fragments arranged by size: 5331 419 47 EcoRI G'AATT_C Cuts at: 2906 3106 3484 2906 Size: 200 378 5219 Fragments arranged by size: 5219 378 200 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 2964 4944 1678 Size: 1286 1980 2531 Fragments arranged by size: 2531 1980 1286 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2157 2189 4438 4470 4512 Size: 32 135 32 585 32 2249 32 42 Cuts at: 4512 4544 1373 Size: 32 2626 Fragments arranged by size: 2626 2249 585 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2233 3141 3387 669 Size: 988 281 295 908 246 3079 Fragments arranged by size: 3079 988 908 295 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5797 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2616 4911 5095 1305 Size: 1311 2295 184 2007 Fragments arranged by size: 2295 2007 1311 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2414 2528 10 Size: 54 1425 343 369 213 114 3279 Fragments arranged by size: 3279 1425 369 343 213 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2057 2477 2534 3078 3755 4114 4273 Size: 648 891 420 57 544 677 359 159 Cuts at: 4273 518 Size: 2042 Fragments arranged by size: 2042 891 677 648 544 420 359 159 57 MspA1I CmG'CkG Cuts at: 1606 3213 3890 5038 5283 1606 Size: 1607 677 1148 245 2120 Fragments arranged by size: 2120 1607 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4306 Fragments arranged by size: 4306 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2464 3155 4700 1132 Size: 97 1235 691 1545 2229 Fragments arranged by size: 2229 1545 1235 691 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2136 3091 1108 Size: 1028 955 3814 Fragments arranged by size: 3814 1028 955 PfoI T'CCnGG_A Cuts at: 2821 2821 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2333 2333 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 2783 1613 Size: 1170 4627 PsiI TTA'TAA Cuts at: 2106 3367 2106 Size: 1261 4536 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2655 2687 2655 Size: 32 5765 PstI C_TGCA'G Cuts at: 830 2287 3149 830 Size: 1457 862 3478 Fragments arranged by size: 3478 1457 862 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 2283 2608 3145 4280 4961 5152 826 Size: 219 1238 325 537 1135 681 191 1471 Fragments arranged by size: 1471 1238 1135 681 537 325 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3901 4802 5064 820 Size: 197 926 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 2464 3155 1132 Size: 1332 691 3774 Fragments arranged by size: 3774 1332 691 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2004 3236 513 Size: 552 939 1232 3074 Fragments arranged by size: 3074 1232 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2528 3824 3841 3994 4179 4624 325 Size: 1255 948 1296 17 153 185 445 1498 Fragments arranged by size: 1498 1296 1255 948 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5757 5789 5757 Size: 32 5765 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 2842 3133 2842 Size: 291 5506 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2644 2644 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 2888 3926 2888 Size: 1038 4759 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI