################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AfaI AflII AflIII AgeI AhdI AocI Aor51HI AscI Asp700I Asp718I AspEI AspI AviII BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BsaAI BsaBI BsaHI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRV EcoT22I FseI FspI HaeII Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI PvuII RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 343 TCCGGA T^CCGGA Alw44I 803 GTGCAC G^TGCAC AlwNI 460 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 728 GGGCCC GGGCC^C ApaLI 803 GTGCAC G^TGCAC AseI 409 ATTAAT AT^TAAT AsnI 409 ATTAAT AT^TAAT AvaI 104 CTCGGG C^[CT]CG[GA]G AvaII 141 GGACC G^G[AT]CC AvrII 114 CCTAGG C^CTAGG BalI 719 TGGCCA TGG^CCA BamHI 7 GGATCC G^GATCC BglI 721 GCCAATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 114 CCTAGG C^CTAGG BlpI 211 GCTCAGC GC^T[ACGT]AGC Bpu1102I 211 GCTCAGC GC^T[ACGT]AGC BsaWI 343 TCCGGA [AT]^CCGG[AT] BseAI 343 TCCGGA T^CCGGA BseRI 271 GAGGAG GAGGAG(10/8) BsgI 325 CTGCAC GTGCAG(16/14) BsiEI 206 CGGCCG CG[GA][CT]^CG BsoBI 104 CTCGGG C^[CT]CG[GA]G BspEI 343 TCCGGA T^CCGGA BspMI 628 GCAGGT ACCTGC(4/8) BsrBI 209 CCGCTC CCGCTC(-3/-3) BstAPI 574 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 7 GGATCC [GA]^GATC[CT] Cac8I 920 GCGTGC GC[ACGT]^[ACGT]GC CelII 211 GCTCAGC GC^T[ACGT]AGC EagI 206 CGGCCG C^GGCCG EclXI 206 CGGCCG C^GGCCG Eco52I 206 CGGCCG C^GGCCG EcoRI 990 GAATTC G^AATTC HgaI 293 GACGC GACGC(5/10) MaeII 944 ACGT A^CGT MaeIII 742 GTGAC ^GT[ACGT]AC MflI 7 GGATCC [GA]^GATC[CT] MluNI 719 TGGCCA TGG^CCA MroI 343 TCCGGA T^CCGGA MscI 719 TGGCCA TGG^CCA MseI 410 TTAA T^TAA MspA1I 147 CCGCTG C[AC]G^C[GT]G NcoI 288 CCATGG C^CATGG PflMI 459 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 639 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 409 ATTAAT AT^TAAT SacI 955 GAGCTC GAGCT^C SfaNI 655 GATGC GCATC(5/9) SfiI 720 GGCCAATGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 256 CTCAAG C^T[CT][GA]AG TaqI 44 TCGA T^CGA Tru9I 410 TTAA T^TAA Tsp45I 742 GTGAC ^GT[GC]AC TspRI 764 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 44 TCGA T^CGA Van91I 459 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 7 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 83 CGCG CG^CG 159 CGCG BbsI 708 GAAGAC GAAGAC(2/6) 844 GAAGAC BcnI 4 CCGGG CC^[GC]GG 267 CCGGG BfaI 115 CTAG C^TAG 421 CTAG BpuAI 708 GAAGAC GAAGAC(2/6) 844 GAAGAC BsrDI 450 CATTGC GCAATG(2/0) 925 CATTGC BsrI 765 ACTGG ACTGG(1/-1) 387 CCAGT BstUI 83 CGCG CG^CG 159 CGCG CfoI 84 GCGC GCG^C 126 GCGC DdeI 212 CTCAG C^T[ACGT]AG 733 CTGAG DraII 727 GGGGCCC [GA]G^G[ACGT]CC[CT] 728 GGGCCCT DsaI 230 CCGTGG C^C[GA][CT]GG 288 CCATGG EaeI 206 CGGCCG [CT]^GGCC[GA] 719 TGGCCA EcoNI 351 CCTAAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 531 CCTCAAAGAGG EcoO109I 727 GGGGCCC [GA]G^G[ACGT]CC[CT] 728 GGGCCCT EcoT14I 114 CCTAGG C^C[AT][AT]GG 288 CCATGG FauI 69 GCGGG CCCGC(4/6) 160 GCGGG HhaI 84 GCGC GCG^C 126 GCGC HinP1I 84 GCGC G^CGC 126 GCGC HincII 336 GTTGAC GT[CT]^[GA]AC 685 GTCAAC HindII 336 GTTGAC GT[CT]^[GA]AC 685 GTCAAC MaeI 115 CTAG C^TAG 421 CTAG MslI 478 CACTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1033 CATCCTGATG MvnI 83 CGCG CG^CG 159 CGCG NciI 4 CCGGG CC^[GC]GG 267 CCGGG StuI 348 AGGCCT AGG^CCT 524 AGGCCT StyI 114 CCTAGG C^C[AT][AT]GG 288 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI BanII BbvI BclI BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BssKI BstF5I BstNI Cfr13I DpnII EcoRII FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RcaI Sau3AI Sau96I ScrFI TseI Tsp509I