(Circular) (Six-base) MAPSORT of: pFN21AE4339.seq Check: 6466 from: 1 to: 5758 pFN21AE4339 5758 bp With 182 enzymes: SgfI * March 28, 2012 13:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 2898 3821 5205 5473 1632 Size: 458 808 923 1384 268 1917 Fragments arranged by size: 1917 1384 923 808 458 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 4657 5634 1203 Size: 3454 977 1327 Fragments arranged by size: 3454 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2264 1184 Size: 1080 4678 AlwNI CAG_nnn'CTG Cuts at: 2470 3501 5073 2470 Size: 1031 1572 3155 Fragments arranged by size: 3155 1572 1031 ApaI G_GGCC'C Cuts at: 2737 2737 Size: 5758 ApaLI G'TGCA_C Cuts at: 2808 3815 4971 2808 Size: 1007 1156 3595 Fragments arranged by size: 3595 1156 1007 ApoI r'AATT_y Cuts at: 1493 2561 2620 2995 3067 3293 3445 5554 1493 Size: 1068 59 375 72 226 152 2109 1697 Fragments arranged by size: 2109 1697 1068 375 226 152 72 59 AseI AT'TA_AT Cuts at: 160 1032 2415 4312 160 Size: 872 1383 1897 1606 Fragments arranged by size: 1897 1606 1383 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2109 3083 1870 Size: 73 166 974 4545 Fragments arranged by size: 4545 974 166 73 AvrII C'CTAG_G Cuts at: 2119 2119 Size: 5758 BamHI G'GATC_C Cuts at: 2012 3088 3455 2012 Size: 1076 367 4315 Fragments arranged by size: 4315 1076 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3079 4534 618 Size: 325 217 627 1292 1455 1842 Fragments arranged by size: 1842 1455 1292 627 325 217 BanII G_rGCy'C Cuts at: 1391 2030 2737 2964 3077 1391 Size: 639 707 227 113 4072 Fragments arranged by size: 4072 707 639 227 113 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2720 2856 928 Size: 971 821 136 3830 Fragments arranged by size: 3830 971 821 136 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3949 3983 1903 Size: 34 50 34 1928 34 3678 Fragments arranged by size: 3678 1928 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 2314 2496 2700 3046 1548 Size: 766 182 204 346 4260 Fragments arranged by size: 4260 766 346 204 182 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2732 4369 136 Size: 107 122 71 631 605 1060 1637 1525 Fragments arranged by size: 1637 1525 1060 631 605 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 2217 3175 2217 Size: 958 4800 Bme1580I G_kGCm'C Cuts at: 1790 2737 2812 3819 4975 1790 Size: 947 75 1007 1156 2573 Fragments arranged by size: 2573 1156 1007 947 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5758 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 4447 442 Size: 416 53 83 399 24 2072 958 1753 Fragments arranged by size: 2072 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2176 2375 2492 2933 4418 1239 Size: 937 199 117 441 1485 2579 Fragments arranged by size: 2579 1485 937 441 199 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2246 3883 4748 5046 2246 Size: 1637 865 298 2958 Fragments arranged by size: 2958 1637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2057 2108 2222 4421 882 Size: 617 558 51 114 2199 2219 Fragments arranged by size: 2219 2199 617 558 114 51 BsaAI yAC'GTr Cuts at: 493 1204 3549 493 Size: 711 2345 2702 Fragments arranged by size: 2702 2345 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 3947 4582 275 Size: 53 83 186 936 2032 382 635 1451 Fragments arranged by size: 2032 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2348 4191 4589 4863 5010 5421 2348 Size: 1843 398 274 147 411 2685 Fragments arranged by size: 2685 1843 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2468 2498 1178 Size: 30 165 30 1065 30 4438 Fragments arranged by size: 4438 1065 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2291 1186 Size: 1105 4653 BseYI C'CCAG_C Cuts at: 4961 5457 4961 Size: 496 5262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2315 1163 Size: 573 579 4606 Fragments arranged by size: 4606 579 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2214 3208 3969 4997 1540 Size: 275 186 213 994 761 1028 2301 Fragments arranged by size: 2301 1028 994 761 275 213 186 BsiHKAI G_wGCw'C Cuts at: 2262 2478 2590 2812 2964 3819 3904 4975 2262 Size: 216 112 222 152 855 85 1071 3045 Fragments arranged by size: 3045 1071 855 222 216 152 112 85 BsmI GAATG_Cn' Cuts at: 3269 3362 3269 Size: 93 5665 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2262 2478 2590 2737 2812 2964 Size: 399 240 232 216 112 147 75 152 Cuts at: 2964 3077 3819 3904 4975 1391 Size: 113 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 240 232 216 152 147 113 112 85 75 BspEI T'CCGG_A Cuts at: 2348 5421 2348 Size: 3073 2685 BspHI T'CATG_A Cuts at: 2499 2605 2652 3647 5377 2499 Size: 106 47 995 1730 2880 Fragments arranged by size: 2880 1730 995 106 47 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2624 3113 844 Size: 1780 489 3489 Fragments arranged by size: 3489 1780 489 BsrBI CCG'CTC Cuts at: 1394 1823 2216 3645 1394 Size: 429 393 1429 3507 Fragments arranged by size: 3507 1429 429 393 BsrDI GCAATG_nn' Cuts at: 65 1248 2454 2929 4253 4427 65 Size: 1183 1206 475 1324 174 1396 Fragments arranged by size: 1396 1324 1206 1183 475 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4402 4589 1700 Size: 110 2592 187 2869 Fragments arranged by size: 2869 2592 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 2585 794 Size: 1791 3967 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstYI r'GATC_y Cuts at: 1972 2012 3088 3455 3840 3857 5309 5395 5407 Size: 40 1076 367 385 17 1452 86 12 Cuts at: 5407 5753 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1076 385 367 346 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2235 2293 513 Size: 552 1170 58 3978 Fragments arranged by size: 3978 1170 552 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5019 Fragments arranged by size: 5019 683 56 BtsI GCAGTG_nn' Cuts at: 3274 4068 4088 3274 Size: 794 20 4944 Fragments arranged by size: 4944 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2971 3006 529 Size: 35 2407 35 3281 Fragments arranged by size: 3281 2407 35 35 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2724 3205 Size: 54 1425 50 275 18 381 513 481 Cuts at: 3205 4094 5723 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 513 481 381 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2211 3205 1537 Size: 275 399 994 4090 Fragments arranged by size: 4090 994 399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3688 1353 Size: 2335 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4345 4719 4865 387 Size: 1064 138 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2962 2962 Size: 5758 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2176 2375 2492 2898 2933 3821 Size: 393 458 86 199 117 406 35 888 Cuts at: 3821 4418 5205 5473 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 888 787 597 458 406 393 268 199 117 86 35 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2540 2360 Size: 180 5578 EcoO109I rG'GnC_Cy Cuts at: 2733 2734 2733 Size: 1 5757 EcoRI G'AATT_C Cuts at: 2995 3067 3445 2995 Size: 72 378 5308 Fragments arranged by size: 5308 378 72 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 4905 1678 Size: 3227 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2200 2232 2740 2772 4399 Size: 32 135 32 628 32 508 32 1627 Cuts at: 4399 4431 4473 4505 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1627 628 508 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2343 2692 3102 3348 669 Size: 988 281 405 349 410 246 3079 Fragments arranged by size: 3079 988 410 405 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 3006 4872 5056 1305 Size: 1116 585 1866 184 2007 Fragments arranged by size: 2007 1866 1116 585 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2726 10 Size: 54 1425 343 894 3042 Fragments arranged by size: 3042 1425 894 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2487 3042 3716 4075 4234 518 Size: 648 1321 555 674 359 159 2042 Fragments arranged by size: 2042 1321 674 648 555 359 159 MspA1I CmG'CkG Cuts at: 1606 2154 3174 3851 4999 5244 1606 Size: 548 1020 677 1148 245 2120 Fragments arranged by size: 2120 1148 1020 677 548 245 NcoI C'CATG_G Cuts at: 513 1065 2293 513 Size: 552 1228 3978 Fragments arranged by size: 3978 1228 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 3116 4661 1132 Size: 97 1887 1545 2229 Fragments arranged by size: 2229 1887 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 2470 1108 Size: 1362 4396 PfoI T'CCnGG_A Cuts at: 2271 2271 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2746 2778 3827 3859 5340 5372 2746 Size: 32 1049 32 1481 32 3132 Fragments arranged by size: 3132 1481 1049 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2648 1613 Size: 1035 4723 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PspOMI G'GGCC_C Cuts at: 2733 2733 Size: 5758 PstI C_TGCA'G Cuts at: 830 3110 830 Size: 2280 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 SacI G_AGCT'C Cuts at: 2964 2964 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SfcI C'TryA_G Cuts at: 826 1045 3106 4241 4922 5113 826 Size: 219 2061 1135 681 191 1471 Fragments arranged by size: 2061 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2732 2732 Size: 5758 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 3862 4763 5025 820 Size: 197 926 318 1601 901 262 1553 Fragments arranged by size: 1601 1553 926 901 318 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 2355 2531 1517 Size: 838 176 4744 Fragments arranged by size: 4744 838 176 StyI C'CwwG_G Cuts at: 513 1065 2119 2293 3197 513 Size: 552 1054 174 904 3074 Fragments arranged by size: 3074 1054 904 552 174 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3785 3802 3955 4140 4585 325 Size: 1255 2205 17 153 185 445 1498 Fragments arranged by size: 2205 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4004 5716 96 Size: 274 80 33 51 1443 2027 1712 138 Fragments arranged by size: 2027 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2468 2500 5718 5750 2468 Size: 32 3218 32 2476 Fragments arranged by size: 3218 2476 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI