Restriction map for pFN21AE4339
 (Linear) (Six-base) MAPSORT of: KIEE4339.seq  Check: 6527  from: 1  to: 1053



KIEE4339.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 17:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     893    1053 
   Size:         85     808     160

  Fragments arranged by size: 

                 808     160      85
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     259    1053 
   Size:        259     794
 
AlwNI CAG_nnn'CTG

Cuts at:      0     465    1053 
   Size:        465     588
 
ApaI G_GGCC'C

Cuts at:      0     732    1053 
   Size:        732     321
 
ApaLI G'TGCA_C

Cuts at:      0     803    1053 
   Size:        803     250
 
ApoI r'AATT_y

Cuts at:      0     556     615     990    1053 
   Size:        556      59     375      63

  Fragments arranged by size: 

                 556     375      63      59
 
AseI AT'TA_AT

Cuts at:      0     410    1053 
   Size:        410     643
 
AvaI C'yCGr_G

Cuts at:      0     104    1053 
   Size:        104     949
 
AvrII C'CTAG_G

Cuts at:      0     114    1053 
   Size:        114     939
 
BamHI G'GATC_C

Cuts at:      0       7    1053 
   Size:          7    1046
 
BanII G_rGCy'C

Cuts at:      0      25     732     959    1053 
   Size:         25     707     227      94

  Fragments arranged by size: 

                 707     227      94      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     715     851    1053 
   Size:        715     136     202

  Fragments arranged by size: 

                 715     202     136
 
BclI T'GATC_A

Cuts at:      0     309     491     695    1041    1053 
   Size:        309     182     204     346      12

  Fragments arranged by size: 

                 346     309     204     182      12
 
BglI GCCn_nnn'nGGC

Cuts at:      0     727    1053 
   Size:        727     326
 
BlpI GC'TnA_GC

Cuts at:      0     212    1053 
   Size:        212     841
 
Bme1580I G_kGCm'C

Cuts at:      0     732     807    1053 
   Size:        732      75     246

  Fragments arranged by size: 

                 732     246      75
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     370     487     928    1053 
   Size:        171     199     117     441     125

  Fragments arranged by size: 

                 441     199     171     125     117
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241    1053 
   Size:        241     812
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      52     103     217    1053 
   Size:         52      51     114     836

  Fragments arranged by size: 

                 836     114      52      51
 
BsaWI w'CCGG_w

Cuts at:      0     343    1053 
   Size:        343     710
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     463     493    1053 
   Size:        463      30     560

  Fragments arranged by size: 

                 560     463      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     286    1053 
   Size:        286     767
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310    1053 
   Size:        310     743
 
BsiEI CG_ry'CG

Cuts at:      0     209    1053 
   Size:        209     844
 
BsiHKAI G_wGCw'C

Cuts at:      0     257     473     585     807     959    1053 
   Size:        257     216     112     222     152      94

  Fragments arranged by size: 

                 257     222     216     152     112      94
 
Bsp1286I G_dGCh'C

Cuts at:      0      25     257     473     585     732     807     959    1053 
   Size:         25     232     216     112     147      75     152      94

  Fragments arranged by size: 

                 232     216     152     147     112      94      75      25
            
 
BspEI T'CCGG_A

Cuts at:      0     343    1053 
   Size:        343     710
 
BspHI T'CATG_A

Cuts at:      0     494     600     647    1053 
   Size:        494     106      47     406

  Fragments arranged by size: 

                 494     406     106      47
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     619    1053 
   Size:        619     434
 
BsrBI CCG'CTC

Cuts at:      0     211    1053 
   Size:        211     842
 
BsrDI GCAATG_nn'

Cuts at:      0     449     924    1053 
   Size:        449     475     129

  Fragments arranged by size: 

                 475     449     129
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     580    1053 
   Size:        580     473
 
BstYI r'GATC_y

Cuts at:      0       7    1053 
   Size:          7    1046
 
BtgI C'CryG_G

Cuts at:      0     230     288    1053 
   Size:        230      58     765

  Fragments arranged by size: 

                 765     230      58
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     966    1001    1053 
   Size:        966      35      52

  Fragments arranged by size: 

                 966      52      35
 
EaeI y'GGCC_r

Cuts at:      0     206     719    1053 
   Size:        206     513     334

  Fragments arranged by size: 

                 513     334     206
 
EagI C'GGCC_G

Cuts at:      0     206    1053 
   Size:        206     847
 
EcoICRI GAG'CTC

Cuts at:      0     957    1053 
   Size:        957      96
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      85     171     370     487     893     928    1053 
   Size:         85      86     199     117     406      35     125

  Fragments arranged by size: 

                 406     199     125     117      86      85      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     355     535    1053 
   Size:        355     180     518

  Fragments arranged by size: 

                 518     355     180
 
EcoO109I rG'GnC_Cy

Cuts at:      0     728     729    1053 
   Size:        728       1     324

  Fragments arranged by size: 

                 728     324       1
 
EcoRI G'AATT_C

Cuts at:      0     990    1053 
   Size:        990      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     195     227     735     767    1053 
   Size:        195      32     508      32     286

  Fragments arranged by size: 

                 508     286     195      32      32
 
HincII GTy'rAC

Cuts at:      0     338     687    1053 
   Size:        338     349     366

  Fragments arranged by size: 

                 366     349     338
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     416    1001    1053 
   Size:        416     585      52

  Fragments arranged by size: 

                 585     416      52
 
MscI TGG'CCA

Cuts at:      0     721    1053 
   Size:        721     332
 
MslI CAynn'nnrTG

Cuts at:      0     482    1037    1053 
   Size:        482     555      16

  Fragments arranged by size: 

                 555     482      16
 
MspA1I CmG'CkG

Cuts at:      0     149    1053 
   Size:        149     904
 
NcoI C'CATG_G

Cuts at:      0     288    1053 
   Size:        288     765
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     465    1053 
   Size:        465     588
 
PfoI T'CCnGG_A

Cuts at:      0     266    1053 
   Size:        266     787
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     741     773    1053 
   Size:        741      32     280

  Fragments arranged by size: 

                 741     280      32
 
PshAI GACnn'nnGTC

Cuts at:      0     643    1053 
   Size:        643     410
 
PspOMI G'GGCC_C

Cuts at:      0     728    1053 
   Size:        728     325
 
SacI G_AGCT'C

Cuts at:      0     959    1053 
   Size:        959      94
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     727    1053 
   Size:        727     326
 
SmlI C'TyrA_G

Cuts at:      0     256    1053 
   Size:        256     797
 
StuI AGG'CCT

Cuts at:      0     350     526    1053 
   Size:        350     176     527

  Fragments arranged by size: 

                 527     350     176
 
StyI C'CwwG_G

Cuts at:      0     114     288    1053 
   Size:        114     174     765

  Fragments arranged by size: 

                 765     174     114
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     463     495    1053 
   Size:        463      32     558

  Fragments arranged by size: 

                 558     463      32
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI
    AvrII    BamHI    BanII     BbsI     BclI     BglI     BlpI Bme1580I
     BpmI    BpuEI     BsaI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI   BstAPI
    BstYI     BtgI    CspCI     EaeI     EagI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    Hin4I   HincII     MmeI     MscI     MslI   MspA1I
     NcoI    PflMI     PfoI     PpiI    PshAI   PspOMI     SacI     SfiI
     SmlI     StuI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     AscI    AsiSI     BaeI     BanI
     BbeI    BbvCI     BcgI    BciVI    BfrBI    BglII    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BseYI    BsiWI
     BsmI    BsmBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EarI     EciI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI    PpuMI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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