(Linear) (Six-base) MAPSORT of: KIEE4339.seq Check: 6527 from: 1 to: 1053 KIEE4339.orf With 182 enzymes: SgfI * March 22, 2012 17:46 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 893 1053 Size: 85 808 160 Fragments arranged by size: 808 160 85 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 259 1053 Size: 259 794 AlwNI CAG_nnn'CTG Cuts at: 0 465 1053 Size: 465 588 ApaI G_GGCC'C Cuts at: 0 732 1053 Size: 732 321 ApaLI G'TGCA_C Cuts at: 0 803 1053 Size: 803 250 ApoI r'AATT_y Cuts at: 0 556 615 990 1053 Size: 556 59 375 63 Fragments arranged by size: 556 375 63 59 AseI AT'TA_AT Cuts at: 0 410 1053 Size: 410 643 AvaI C'yCGr_G Cuts at: 0 104 1053 Size: 104 949 AvrII C'CTAG_G Cuts at: 0 114 1053 Size: 114 939 BamHI G'GATC_C Cuts at: 0 7 1053 Size: 7 1046 BanII G_rGCy'C Cuts at: 0 25 732 959 1053 Size: 25 707 227 94 Fragments arranged by size: 707 227 94 25 BbsI GAAGACnn'nnnn_ Cuts at: 0 715 851 1053 Size: 715 136 202 Fragments arranged by size: 715 202 136 BclI T'GATC_A Cuts at: 0 309 491 695 1041 1053 Size: 309 182 204 346 12 Fragments arranged by size: 346 309 204 182 12 BglI GCCn_nnn'nGGC Cuts at: 0 727 1053 Size: 727 326 BlpI GC'TnA_GC Cuts at: 0 212 1053 Size: 212 841 Bme1580I G_kGCm'C Cuts at: 0 732 807 1053 Size: 732 75 246 Fragments arranged by size: 732 246 75 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 171 370 487 928 1053 Size: 171 199 117 441 125 Fragments arranged by size: 441 199 171 125 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 241 1053 Size: 241 812 BsaI GGTCTCn'nnnn_ Cuts at: 0 52 103 217 1053 Size: 52 51 114 836 Fragments arranged by size: 836 114 52 51 BsaWI w'CCGG_w Cuts at: 0 343 1053 Size: 343 710 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 463 493 1053 Size: 463 30 560 Fragments arranged by size: 560 463 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 286 1053 Size: 286 767 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 1053 Size: 310 743 BsiEI CG_ry'CG Cuts at: 0 209 1053 Size: 209 844 BsiHKAI G_wGCw'C Cuts at: 0 257 473 585 807 959 1053 Size: 257 216 112 222 152 94 Fragments arranged by size: 257 222 216 152 112 94 Bsp1286I G_dGCh'C Cuts at: 0 25 257 473 585 732 807 959 1053 Size: 25 232 216 112 147 75 152 94 Fragments arranged by size: 232 216 152 147 112 94 75 25 BspEI T'CCGG_A Cuts at: 0 343 1053 Size: 343 710 BspHI T'CATG_A Cuts at: 0 494 600 647 1053 Size: 494 106 47 406 Fragments arranged by size: 494 406 106 47 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 619 1053 Size: 619 434 BsrBI CCG'CTC Cuts at: 0 211 1053 Size: 211 842 BsrDI GCAATG_nn' Cuts at: 0 449 924 1053 Size: 449 475 129 Fragments arranged by size: 475 449 129 BstAPI GCAn_nnn'nTGC Cuts at: 0 580 1053 Size: 580 473 BstYI r'GATC_y Cuts at: 0 7 1053 Size: 7 1046 BtgI C'CryG_G Cuts at: 0 230 288 1053 Size: 230 58 765 Fragments arranged by size: 765 230 58 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 966 1001 1053 Size: 966 35 52 Fragments arranged by size: 966 52 35 EaeI y'GGCC_r Cuts at: 0 206 719 1053 Size: 206 513 334 Fragments arranged by size: 513 334 206 EagI C'GGCC_G Cuts at: 0 206 1053 Size: 206 847 EcoICRI GAG'CTC Cuts at: 0 957 1053 Size: 957 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 171 370 487 893 928 1053 Size: 85 86 199 117 406 35 125 Fragments arranged by size: 406 199 125 117 86 85 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 355 535 1053 Size: 355 180 518 Fragments arranged by size: 518 355 180 EcoO109I rG'GnC_Cy Cuts at: 0 728 729 1053 Size: 728 1 324 Fragments arranged by size: 728 324 1 EcoRI G'AATT_C Cuts at: 0 990 1053 Size: 990 63 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 195 227 735 767 1053 Size: 195 32 508 32 286 Fragments arranged by size: 508 286 195 32 32 HincII GTy'rAC Cuts at: 0 338 687 1053 Size: 338 349 366 Fragments arranged by size: 366 349 338 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 416 1001 1053 Size: 416 585 52 Fragments arranged by size: 585 416 52 MscI TGG'CCA Cuts at: 0 721 1053 Size: 721 332 MslI CAynn'nnrTG Cuts at: 0 482 1037 1053 Size: 482 555 16 Fragments arranged by size: 555 482 16 MspA1I CmG'CkG Cuts at: 0 149 1053 Size: 149 904 NcoI C'CATG_G Cuts at: 0 288 1053 Size: 288 765 PflMI CCAn_nnn'nTGG Cuts at: 0 465 1053 Size: 465 588 PfoI T'CCnGG_A Cuts at: 0 266 1053 Size: 266 787 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 741 773 1053 Size: 741 32 280 Fragments arranged by size: 741 280 32 PshAI GACnn'nnGTC Cuts at: 0 643 1053 Size: 643 410 PspOMI G'GGCC_C Cuts at: 0 728 1053 Size: 728 325 SacI G_AGCT'C Cuts at: 0 959 1053 Size: 959 94 SfiI GGCCn_nnn'nGGCC Cuts at: 0 727 1053 Size: 727 326 SmlI C'TyrA_G Cuts at: 0 256 1053 Size: 256 797 StuI AGG'CCT Cuts at: 0 350 526 1053 Size: 350 176 527 Fragments arranged by size: 527 350 176 StyI C'CwwG_G Cuts at: 0 114 288 1053 Size: 114 174 765 Fragments arranged by size: 765 174 114 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 463 495 1053 Size: 463 32 558 Fragments arranged by size: 558 463 32 Enzymes that do cut: AcuI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BamHI BanII BbsI BclI BglI BlpI Bme1580I BpmI BpuEI BsaI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BstAPI BstYI BtgI CspCI EaeI EagI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII MmeI MscI MslI MspA1I NcoI PflMI PfoI PpiI PshAI PspOMI SacI SfiI SmlI StuI StyI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI AscI AsiSI BaeI BanI BbeI BbvCI BcgI BciVI BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BseYI BsiWI BsmI BsmBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EarI EciI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpuMI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI