Restriction map for
pFN21AE0583
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# NO CUT ENZYMES ( 155 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BcnI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I CelII CfoI ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FokI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NarI NciI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI TaqI
Tru9I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 693 GACGTC GACGT^C
AcsI 413 GAATTT [GA]^AATT[CT]
AcyI 693 GACGTC G[GA]^CG[CT]C
AlwI 90 GATCC GGATC(4/5)
AocI 595 CCTCAGG CC^T[ACGT]AGG
ApoI 413 GAATTT [GA]^AATT[CT]
AspHI 64 GAGCTC G[AT]GC[AT]^C
BanI 45 GGTGCC G^G[CT][GA]CC
BbsI 519 GAAGAC GAAGAC(2/6)
BciVI 463 GGATAC GTATCC(6/5)
BclI 749 TGATCA T^GATCA
BfaI 212 CTAG C^TAG
BglI 605 GCCCCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 519 GAAGAC GAAGAC(2/6)
BsaHI 693 GACGTC G[GA]^CG[CT]C
BsaI 565 GAGACC GGTCTC(1/5)
BseRI 760 CTCCTC GAGGAG(10/8)
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
BsiYI 779 CCTGGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 779 CCTGGCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 691 GAGACG CGTCTC(1/5)
BsmI 148 GAATGC GAATGC(1/-1)
Bsp1407I 199 TGTACA T^GTACA
BspMI 11 ACCTGC ACCTGC(4/8)
BsrFI 730 GCCGGC [GA]^CCGG[CT]
BsrGI 199 TGTACA T^GTACA
BstYI 89 AGATCC [GA]^GATC[CT]
Bsu36I 595 CCTCAGG CC^T[ACGT]AGG
Cfr10I 730 GCCGGC [GA]^CCGG[CT]
DraII 483 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 728 TGGCCG [CT]^GGCC[GA]
Eco81I 595 CCTCAGG CC^T[ACGT]AGG
EcoO109I 483 AGGTCCT [GA]G^G[ACGT]CC[CT]
FbaI 749 TGATCA T^GATCA
HapII 731 CCGG C^CGG
Hin1I 693 GACGTC G[GA]^CG[CT]C
HinfI 4 GATTC G^A[ACGT]TC
HpaII 731 CCGG C^CGG
MaeI 212 CTAG C^TAG
MflI 89 AGATCC [GA]^GATC[CT]
MspA1I 784 CAGCGG C[AC]G^C[GT]G
MspI 731 CCGG C^CGG
NaeI 730 GCCGGC GCC^GGC
NdeI 546 CATATG CA^TATG
NgoMIV 730 GCCGGC G^CCGGC
PpuMI 483 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 372 CTGCAG CTGCA^G
SacI 64 GAGCTC GAGCT^C
SexAI 716 ACCAGGT A^CC[AT]GGT
SfcI 372 CTGCAG C^T[GA][CT]AG
SspBI 199 TGTACA T^GTACA
TfiI 4 GATTC G^A[AT]TC
Tsp509I 414 AATT ^AATT
XhoII 89 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AfaI 200 GTAC GT^AC
714 GTAC
BsmFI 24 GGGAC GGGAC(10/14)
227 GGGAC
BsrI 343 CCAGT ACTGG(1/-1)
569 CCAGT
DpnII 90 GATC ^GATC
750 GATC
EarI 586 GAAGAG CTCTTC(1/4)
798 GAAGAG
FauI 81 CCCGC CCCGC(4/6)
179 GCGGG
Ksp632I 586 GAAGAG CTCTTC(1/4)
798 GAAGAG
MaeII 34 ACGT A^CGT
694 ACGT
MboI 90 GATC ^GATC
750 GATC
MslI 39 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
318 CACAGAAATG
MwoI 605 GCCCCGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
774 GCTCACCTGGC
NdeII 90 GATC ^GATC
750 GATC
RsaI 200 GTAC GT^AC
714 GTAC
Sau3AI 90 GATC ^GATC
750 GATC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BsaJI BsmAI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI DsaI
EcoRII EcoT14I Fnu4HI HaeIII HgaI
HphI ItaI MaeIII MboII MnlI
MvaI NcoI NlaIII NlaIV Sau96I
ScrFI StyI TseI Tsp45I TspRI