Restriction map for
pFN21AE0583
(Linear) (Six-base) MAPSORT of: KIEE0583.seq Check: 6579 from: 1 to: 816
KIEE0583.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AatII G_ACGT'C
Cuts at: 0 697 816
Size: 697 119
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 816
Size: 314 502
ApoI r'AATT_y
Cuts at: 0 413 816
Size: 413 403
BanI G'GyrC_C
Cuts at: 0 45 816
Size: 45 771
BanII G_rGCy'C
Cuts at: 0 68 474 776 816
Size: 68 406 302 40
Fragments arranged by size:
406 302 68 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 526 816
Size: 526 290
BciVI GTATCCnnnnn_n'
Cuts at: 0 457 816
Size: 457 359
BclI T'GATC_A
Cuts at: 0 749 816
Size: 749 67
BglI GCCn_nnn'nGGC
Cuts at: 0 611 816
Size: 611 205
BsaI GGTCTCn'nnnn_
Cuts at: 0 559 816
Size: 559 257
BsaHI Gr'CG_yC
Cuts at: 0 694 816
Size: 694 122
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 751 816
Size: 751 65
BseYI C'CCAG_C
Cuts at: 0 533 539 816
Size: 533 6 277
Fragments arranged by size:
533 277 6
BsiHKAI G_wGCw'C
Cuts at: 0 68 816
Size: 68 748
BsmI GAATG_Cn'
Cuts at: 0 154 816
Size: 154 662
BsmBI CGTCTCn'nnnn_
Cuts at: 0 685 816
Size: 685 131
Bsp1286I G_dGCh'C
Cuts at: 0 68 474 776 816
Size: 68 406 302 40
Fragments arranged by size:
406 302 68 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 20 816
Size: 20 796
BsrFI r'CCGG_y
Cuts at: 0 730 816
Size: 730 86
BsrGI T'GTAC_A
Cuts at: 0 199 816
Size: 199 617
BstYI r'GATC_y
Cuts at: 0 89 816
Size: 89 727
Bsu36I CC'TnA_GG
Cuts at: 0 596 816
Size: 596 220
BtgI C'CryG_G
Cuts at: 0 113 388 513 686 816
Size: 113 275 125 173 130
Fragments arranged by size:
275 173 130 125 113
BtsI GCAGTG_nn'
Cuts at: 0 369 731 816
Size: 369 362 85
Fragments arranged by size:
369 362 85
EaeI y'GGCC_r
Cuts at: 0 728 816
Size: 728 88
EarI CTCTTCn'nnn_
Cuts at: 0 581 793 816
Size: 581 212 23
Fragments arranged by size:
581 212 23
EcoICRI GAG'CTC
Cuts at: 0 66 816
Size: 66 750
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 816
Size: 314 502
EcoO109I rG'GnC_Cy
Cuts at: 0 484 816
Size: 484 332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 376 816
Size: 376 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 402 434 816
Size: 402 32 382
Fragments arranged by size:
402 382 32
MslI CAynn'nnrTG
Cuts at: 0 43 322 816
Size: 43 279 494
Fragments arranged by size:
494 279 43
MspA1I CmG'CkG
Cuts at: 0 786 816
Size: 786 30
NaeI GCC'GGC
Cuts at: 0 732 816
Size: 732 84
NcoI C'CATG_G
Cuts at: 0 113 388 686 816
Size: 113 275 298 130
Fragments arranged by size:
298 275 130 113
NdeI CA'TA_TG
Cuts at: 0 547 816
Size: 547 269
NgoMIV G'CCGG_C
Cuts at: 0 730 816
Size: 730 86
PpuMI rG'GwC_Cy
Cuts at: 0 484 816
Size: 484 332
PstI C_TGCA'G
Cuts at: 0 376 816
Size: 376 440
SacI G_AGCT'C
Cuts at: 0 68 816
Size: 68 748
SexAI A'CCwGG_T
Cuts at: 0 716 816
Size: 716 100
SfcI C'TryA_G
Cuts at: 0 372 816
Size: 372 444
StyI C'CwwG_G
Cuts at: 0 113 388 686 816
Size: 113 275 298 130
Fragments arranged by size:
298 275 130 113
TatI w'GTAC_w
Cuts at: 0 199 816
Size: 199 617
ZraI GAC'GTC
Cuts at: 0 695 816
Size: 695 121
Enzymes that do cut:
AatII AcuI ApoI BanI BanII BbsI BciVI BclI
BglI BsaI BsaHI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrFI BsrGI BstYI Bsu36I BtgI BtsI
EaeI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I MslI
MspA1I NaeI NcoI NdeI NgoMIV PpuMI PstI SacI
SexAI SfcI StyI TatI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI XmnI