Restriction map for
pFN21AE0578
(Linear) (Six-base) MAPSORT of: KIEE0578.seq Check: 1282 from: 1 to: 621
KIEE0578.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 621
Size: 187 434
AflIII A'CryG_T
Cuts at: 0 388 621
Size: 388 233
ApaLI G'TGCA_C
Cuts at: 0 241 621
Size: 241 380
ApoI r'AATT_y
Cuts at: 0 544 621
Size: 544 77
AvaI C'yCGr_G
Cuts at: 0 512 621
Size: 512 109
AvrII C'CTAG_G
Cuts at: 0 342 621
Size: 342 279
BamHI G'GATC_C
Cuts at: 0 173 621
Size: 173 448
BanI G'GyrC_C
Cuts at: 0 182 621
Size: 182 439
BbeI G_GCGC'C
Cuts at: 0 186 621
Size: 186 435
BbsI GAAGACnn'nnnn_
Cuts at: 0 12 621
Size: 12 609
BciVI GTATCCnnnnn_n'
Cuts at: 0 514 621
Size: 514 107
BclI T'GATC_A
Cuts at: 0 308 621
Size: 308 313
BglI GCCn_nnn'nGGC
Cuts at: 0 206 621
Size: 206 415
BglII A'GATC_T
Cuts at: 0 221 621
Size: 221 400
Bme1580I G_kGCm'C
Cuts at: 0 77 245 621
Size: 77 168 376
Fragments arranged by size:
376 168 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 45 621
Size: 45 576
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 382 621
Size: 382 239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 301 621
Size: 238 63 320
Fragments arranged by size:
320 238 63
Bpu10I CC'TnA_GC
Cuts at: 0 78 621
Size: 78 543
BsaI GGTCTCn'nnnn_
Cuts at: 0 596 621
Size: 596 25
BsaAI yAC'GTr
Cuts at: 0 391 486 621
Size: 391 95 135
Fragments arranged by size:
391 135 95
BsaBI GATnn'nnATC
Cuts at: 0 313 621
Size: 313 308
BsaHI Gr'CG_yC
Cuts at: 0 17 183 621
Size: 17 166 438
Fragments arranged by size:
438 166 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 621
Size: 37 30 554
Fragments arranged by size:
554 37 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 45 374 621
Size: 45 329 247
Fragments arranged by size:
329 247 45
BsiHKAI G_wGCw'C
Cuts at: 0 245 621
Size: 245 376
BsmBI CGTCTCn'nnnn_
Cuts at: 0 65 621
Size: 65 556
Bsp1286I G_dGCh'C
Cuts at: 0 77 245 621
Size: 77 168 376
Fragments arranged by size:
376 168 77
BsrFI r'CCGG_y
Cuts at: 0 205 621
Size: 205 416
BsrGI T'GTAC_A
Cuts at: 0 452 621
Size: 452 169
BstYI r'GATC_y
Cuts at: 0 39 173 221 573 621
Size: 39 134 48 352 48
Fragments arranged by size:
352 134 48 48 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 238 301 621
Size: 187 51 63 320
Fragments arranged by size:
320 187 63 51
EcoO109I rG'GnC_Cy
Cuts at: 0 248 621
Size: 248 373
HaeII r_GCGC'y
Cuts at: 0 111 186 621
Size: 111 75 435
Fragments arranged by size:
435 111 75
KasI G'GCGC_C
Cuts at: 0 182 621
Size: 182 439
MslI CAynn'nnrTG
Cuts at: 0 393 621
Size: 393 228
NarI GG'CG_CC
Cuts at: 0 183 621
Size: 183 438
PfoI T'CCnGG_A
Cuts at: 0 278 404 621
Size: 278 126 217
Fragments arranged by size:
278 217 126
PmlI CAC'GTG
Cuts at: 0 391 621
Size: 391 230
PpuMI rG'GwC_Cy
Cuts at: 0 248 621
Size: 248 373
PstI C_TGCA'G
Cuts at: 0 137 621
Size: 137 484
SanDI GG'GwC_CC
Cuts at: 0 248 621
Size: 248 373
SfcI C'TryA_G
Cuts at: 0 133 569 615 621
Size: 133 436 46 6
Fragments arranged by size:
436 133 46 6
SfoI GGC'GCC
Cuts at: 0 184 621
Size: 184 437
SmaI CCC'GGG
Cuts at: 0 514 621
Size: 514 107
StuI AGG'CCT
Cuts at: 0 567 621
Size: 567 54
StyI C'CwwG_G
Cuts at: 0 29 342 621
Size: 29 313 279
Fragments arranged by size:
313 279 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 162 621
Size: 162 459
TatI w'GTAC_w
Cuts at: 0 452 621
Size: 452 169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 621
Size: 37 32 552
Fragments arranged by size:
552 37 32
XmaI C'CCGG_G
Cuts at: 0 512 621
Size: 512 109
Enzymes that do cut:
AcuI AflIII ApaLI ApoI AvaI AvrII BamHI BanI
BbeI BbsI BciVI BclI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BsiHKAI BsmBI Bsp1286I BsrFI BsrGI BstYI Eco57MI
EcoO109I HaeII KasI MslI NarI PfoI PmlI PpuMI
PstI SanDI SfcI SfoI SmaI StuI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI BaeI BanII BbvCI BcgI BfrBI BlpI BmgBI
BmtI BpuEI BsaWI BseYI BsgI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI