Restriction map for
pFN21AE4277
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# NO CUT ENZYMES ( 75 enzymes) #
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AatII AclI AflIII AgeI AhdI
ApaI AscI AseI AsnI AspEI
AspI AviII AvrII BbrPI BbvCI
BcgI BclI BlnI BsaBI BsiWI
Bsp1407I BspDI BspLU11I BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoRV
FbaI FseI FspI HpaI MamI
MluI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI SexAI SfiI SfuI SmaI
SpeI Sse8387I SspBI SwaI TaqI
Tth111I TthHB8I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 459 GGTACC G^GTACC
AccI 482 GTAGAC GT^[AC][GT]AC
AccIII 19 TCCGGA T^CCGGA
AcyI 442 GGCGCC G[GA]^CG[CT]C
AflII 837 CTTAAG C^TTAAG
AocI 384 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2078 AGCGCT AGC^GCT
Asp718I 459 GGTACC G^GTACC
BbeI 442 GGCGCC GGCGC^C
BfrI 837 CTTAAG C^TTAAG
BglII 2093 AGATCT A^GATCT
BlpI 723 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 723 GCTGAGC GC^T[ACGT]AGC
BsaAI 3104 TACGTA [CT]AC^GT[GA]
BsaHI 442 GGCGCC G[GA]^CG[CT]C
BsaI 674 GGTCTC GGTCTC(1/5)
BseAI 19 TCCGGA T^CCGGA
BsiEI 2213 CGACCG CG[GA][CT]^CG
BspEI 19 TCCGGA T^CCGGA
BssHII 3725 GCGCGC G^CGCGC
BstEII 828 GGTTACC G^GT[ACGT]ACC
BstPI 828 GGTTACC G^GT[ACGT]ACC
Bsu36I 384 CCTGAGG CC^T[ACGT]AGG
CelII 723 GCTGAGC GC^T[ACGT]AGC
DrdI 103 GACGCGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2078 AGCGCT AGC^GCT
Eco81I 384 CCTGAGG CC^T[ACGT]AGG
EcoO65I 828 GGTTACC G^GT[ACGT]ACC
EcoT22I 2588 ATGCAT ATGCA^T
FauI 2670 GCGGG CCCGC(4/6)
Hin1I 442 GGCGCC G[GA]^CG[CT]C
HincII 1192 GTCAAC GT[CT]^[GA]AC
HindII 1192 GTCAAC GT[CT]^[GA]AC
KasI 442 GGCGCC G^GCGCC
KpnI 459 GGTACC GGTAC^C
KspI 29 CCGCGG CCGC^GG
MfeI 930 CAATTG C^AATTG
MroI 19 TCCGGA T^CCGGA
MslI 3120 CATGTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 930 CAATTG C^AATTG
NaeI 3434 GCCGGC GCC^GGC
NarI 442 GGCGCC GG^CGCC
NcoI 527 CCATGG C^CATGG
NgoMIV 3434 GCCGGC G^CCGGC
NsiI 2588 ATGCAT ATGCA^T
PflMI 115 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2359 CTGCAG CTGCA^G
SacI 4 GAGCTC GAGCT^C
SacII 29 CCGCGG CCGC^GG
SfoI 442 GGCGCC GGC^GCC
SgrAI 132 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 3104 TACGTA TAC^GTA
StuI 2813 AGGCCT AGG^CCT
Van91I 115 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3360 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 23 enzymes) #
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Alw44I 1450 GTGCAC G^TGCAC
2696 GTGCAC
ApaLI 1450 GTGCAC G^TGCAC
2696 GTGCAC
AvaI 424 CCCGAG C^[CT]CG[GA]G
3062 CCCGAG
BalI 1994 TGGCCA TGG^CCA
2651 TGGCCA
BamHI 152 GGATCC G^GATCC
1674 GGATCC
BciVI 1242 GTATCC GTATCC(6/5)
1935 GTATCC
BsaWI 19 TCCGGA [AT]^CCGG[AT]
2623 ACCGGA
BsmBI 2541 CGTCTC CGTCTC(1/5)
2301 GAGACG
BsmI 2035 GCATTC GAATGC(1/-1)
2172 GCATTC
BsoBI 424 CCCGAG C^[CT]CG[GA]G
3062 CCCGAG
BspHI 1614 TCATGA T^CATGA
2039 TCATGA
BspMI 3249 GCAGGT ACCTGC(4/8)
3759 GCAGGT
BsrDI 2803 GCAATG GCAATG(2/0)
3767 GCAATG
DraIII 2965 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
3089 CACACAGTG
EcoNI 334 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1923 CCTTATGCAGG
EcoRI 1093 GAATTC G^AATTC
3820 GAATTC
MluNI 1994 TGGCCA TGG^CCA
2651 TGGCCA
MscI 1994 TGGCCA TGG^CCA
2651 TGGCCA
MseI 838 TTAA T^TAA
1301 TTAA
RcaI 1614 TCATGA T^CATGA
2039 TCATGA
ScaI 1465 AGTACT AGT^ACT
3013 AGTACT
SfcI 2359 CTGCAG C^T[GA][CT]AG
2819 CTATAG
Tru9I 838 TTAA T^TAA
1301 TTAA
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# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI SphI
SspI StyI TfiI TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmnI