(Circular) (Six-base) MAPSORT of: pFN21AE4277.seq Check: 6216 from: 1 to: 8545 pFN21AE4277 8545 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6355 278 Size: 53 83 186 936 4819 2468 Fragments arranged by size: 4819 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2488 5888 1937 Size: 551 3400 4594 Fragments arranged by size: 4594 3400 551 Acc65I G'GTAC_C Cuts at: 2464 5866 2464 Size: 3402 5143 AclI AA'CG_TT Cuts at: 1563 6672 7045 1563 Size: 5109 373 3063 Fragments arranged by size: 5109 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2560 2845 3088 3535 4429 4739 5435 5639 Size: 928 285 243 447 894 310 696 204 Cuts at: 5639 6608 7992 8260 1632 Size: 969 1384 268 1917 Fragments arranged by size: 1917 1384 969 928 894 696 447 310 285 268 243 204 AfeI AGC'GCT Cuts at: 4085 4085 Size: 8545 AflII C'TTAA_G Cuts at: 820 1017 2842 820 Size: 197 1825 6523 Fragments arranged by size: 6523 1825 197 AflIII A'CryG_T Cuts at: 1203 7444 8421 1203 Size: 6241 977 1327 Fragments arranged by size: 6241 1327 977 AgeI A'CCGG_T Cuts at: 7376 7376 Size: 8545 AhdI GACnn_n'nnGTC Cuts at: 7274 7274 Size: 8545 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8545 AlwNI CAG_nnn'CTG Cuts at: 3829 3997 4303 5497 5841 6288 7860 3829 Size: 168 306 1194 344 447 1572 4514 Fragments arranged by size: 4514 1572 1194 447 344 306 168 ApaLI G'TGCA_C Cuts at: 3455 4701 6602 7758 3455 Size: 1246 1901 1156 4242 Fragments arranged by size: 4242 1901 1246 1156 ApoI r'AATT_y Cuts at: 1493 3098 3356 3365 5825 5854 6080 6232 8341 Size: 1605 258 9 2460 29 226 152 2109 Cuts at: 8341 1493 Size: 1697 Fragments arranged by size: 2460 2109 1697 1605 258 226 152 29 9 AseI AT'TA_AT Cuts at: 160 1032 7099 160 Size: 872 6067 1606 Fragments arranged by size: 6067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8545 AvaI C'yCGr_G Cuts at: 1870 1943 2429 5067 5870 1870 Size: 73 486 2638 803 4545 Fragments arranged by size: 4545 2638 803 486 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3596 3629 3596 Size: 33 8512 BamHI G'GATC_C Cuts at: 2157 3679 5875 6242 2157 Size: 1522 2196 367 4460 Fragments arranged by size: 4460 2196 1522 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2447 2464 4975 5063 5866 Size: 325 217 627 660 17 2511 88 803 Cuts at: 5866 7321 618 Size: 1455 1842 Fragments arranged by size: 2511 1842 1455 803 660 627 325 217 88 17 BanII G_rGCy'C Cuts at: 1391 2013 2735 5213 5864 1391 Size: 622 722 2478 651 4072 Fragments arranged by size: 4072 2478 722 651 622 BbeI G_GCGC'C Cuts at: 2451 2451 Size: 8545 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4386 4890 5554 928 Size: 971 2487 504 664 3919 Fragments arranged by size: 3919 2487 971 664 504 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6736 6770 1903 Size: 34 4799 34 3678 Fragments arranged by size: 4799 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3258 3951 6437 7647 3258 Size: 693 2486 1210 4156 Fragments arranged by size: 4156 2486 1210 693 BclI T'GATC_A Cuts at: 1548 1548 Size: 8545 BfrBI ATG'CAT Cuts at: 4595 4595 Size: 8545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 2592 5150 Size: 107 122 71 631 605 367 553 2558 Cuts at: 5150 7156 136 Size: 2006 1525 Fragments arranged by size: 2558 2006 1525 631 605 553 367 122 107 71 BglII A'GATC_T Cuts at: 4098 8540 4098 Size: 4442 4103 BlpI GC'TnA_GC Cuts at: 2729 5962 2729 Size: 3233 5312 Bme1580I G_kGCm'C Cuts at: 1790 3459 4705 5068 6606 7762 1790 Size: 1669 1246 363 1538 1156 2573 Fragments arranged by size: 2573 1669 1538 1246 1156 363 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2114 3638 3909 Size: 416 53 83 399 24 697 1524 271 Cuts at: 3909 5031 5285 6276 7234 442 Size: 1122 254 991 958 1753 Fragments arranged by size: 1753 1524 1122 991 958 697 416 399 271 254 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8545 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2098 4975 2098 Size: 2877 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2186 2480 4312 4547 4945 5185 5468 Size: 854 93 294 1832 235 398 240 283 Cuts at: 5468 5543 7205 1239 Size: 75 1662 2579 Fragments arranged by size: 2579 1832 1662 854 398 294 283 240 235 93 75 Bpu10I CC'TnA_GC Cuts at: 4847 4847 Size: 8545 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3425 3578 4566 6670 7535 7833 3425 Size: 153 988 2104 865 298 4137 Fragments arranged by size: 4137 2104 988 865 298 153 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2685 7208 882 Size: 617 1186 4523 2219 Fragments arranged by size: 4523 2219 1186 617 BsaAI yAC'GTr Cuts at: 493 1204 5111 6336 493 Size: 711 3907 1225 2702 Fragments arranged by size: 3907 2702 1225 711 BsaBI GATnn'nnATC Cuts at: 8539 8539 Size: 8545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2448 6352 6734 7369 Size: 53 83 186 936 915 3904 382 635 Cuts at: 7369 275 Size: 1451 Fragments arranged by size: 3904 1451 936 915 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2024 4628 6978 7376 7650 7797 8208 2024 Size: 2604 2350 398 274 147 411 2361 Fragments arranged by size: 2604 2361 2350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2030 2060 2159 2189 2267 Size: 30 165 30 627 30 99 30 78 Cuts at: 2267 2297 1178 Size: 30 7426 Fragments arranged by size: 7426 627 165 99 78 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2043 2407 3449 4345 4471 5549 1186 Size: 857 364 1042 896 126 1078 4182 Fragments arranged by size: 4182 1078 1042 896 857 364 126 BseYI C'CCAG_C Cuts at: 2190 3683 4729 4904 7748 8244 2190 Size: 1493 1046 175 2844 496 2491 Fragments arranged by size: 2844 2491 1493 1046 496 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4709 5183 5495 1163 Size: 573 2973 474 312 4213 Fragments arranged by size: 4213 2973 573 474 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4221 5995 6756 7784 1540 Size: 275 186 2220 1774 761 1028 2301 Fragments arranged by size: 2301 2220 1774 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 3459 4691 4705 6606 6691 7762 2013 Size: 1446 1232 14 1901 85 1071 2796 Fragments arranged by size: 2796 1901 1446 1232 1071 85 14 BsmI GAATG_Cn' Cuts at: 4040 4177 6056 6149 4040 Size: 137 1879 93 6436 Fragments arranged by size: 6436 1879 137 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4300 4552 4300 Size: 252 8293 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2735 3459 4691 4705 5068 5213 Size: 399 223 722 724 1232 14 363 145 Cuts at: 5213 5864 6606 6691 7762 1391 Size: 651 742 85 1071 2174 Fragments arranged by size: 2174 1232 1071 742 724 722 651 399 363 223 145 85 14 BspEI T'CCGG_A Cuts at: 2024 8208 2024 Size: 6184 2361 BspHI T'CATG_A Cuts at: 3619 4044 6434 8164 3619 Size: 425 2390 1730 4000 Fragments arranged by size: 4000 2390 1730 425 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5245 5755 5900 844 Size: 4401 510 145 3489 Fragments arranged by size: 4401 3489 510 145 BsrBI CCG'CTC Cuts at: 1394 1823 2278 5539 5752 6432 1394 Size: 429 455 3261 213 680 3507 Fragments arranged by size: 3507 3261 680 455 429 213 BsrDI GCAATG_nn' Cuts at: 65 1248 4815 5779 7040 7214 65 Size: 1183 3567 964 1261 174 1396 Fragments arranged by size: 3567 1396 1261 1183 964 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2138 5175 5439 7189 7376 1700 Size: 110 328 3037 264 1750 187 2869 Fragments arranged by size: 3037 2869 1750 328 264 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8545 BssHII G'CGCG_C Cuts at: 1925 5730 1925 Size: 3805 4740 BssSI C'ACGA_G Cuts at: 2638 2682 3786 4245 6605 7617 2638 Size: 44 1104 459 2360 1012 3566 Fragments arranged by size: 3566 2360 1104 1012 459 44 BstAPI GCAn_nnn'nTGC Cuts at: 794 4975 5488 5497 794 Size: 4181 513 9 3842 Fragments arranged by size: 4181 3842 513 9 BstBI TT'CG_AA Cuts at: 7349 7349 Size: 8545 BstEII G'GTnAC_C Cuts at: 1184 2833 1184 Size: 1649 6896 BstXI CCAn_nnnn'nTGG Cuts at: 2458 2533 4748 2458 Size: 75 2215 6255 Fragments arranged by size: 6255 2215 75 BstYI r'GATC_y Cuts at: 1972 2157 2758 3679 4098 5034 5875 6242 6627 Size: 185 601 921 419 936 841 367 385 Cuts at: 6627 6644 8096 8182 8194 8540 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 936 921 841 601 419 385 367 346 185 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2390 2390 Size: 8545 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 2532 3835 4221 513 Size: 552 939 30 498 1303 386 4837 Fragments arranged by size: 4837 1303 939 552 498 386 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2398 487 Size: 683 56 1172 6634 Fragments arranged by size: 6634 1172 683 56 BtsI GCAGTG_nn' Cuts at: 2538 3207 3830 4851 6061 6855 6875 2538 Size: 669 623 1021 1210 794 20 4208 Fragments arranged by size: 4208 1210 1021 794 669 623 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4879 4914 529 Size: 35 4315 35 4160 Fragments arranged by size: 4315 4160 35 35 DraI TTT'AAA Cuts at: 5849 5849 Size: 8545 DraIII CAC_nnn'GTG Cuts at: 4975 5099 4975 Size: 124 8421 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2114 7552 809 Size: 507 798 5438 1802 Fragments arranged by size: 5438 1802 798 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2051 3999 4656 Size: 54 1425 50 275 18 221 1948 657 Cuts at: 4656 5151 5992 6881 8510 8 Size: 495 841 889 1629 43 Fragments arranged by size: 1948 1629 1425 889 841 657 495 275 221 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5992 1537 Size: 275 4180 4090 Fragments arranged by size: 4180 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3123 5200 5331 5604 6475 1353 Size: 1770 2077 131 273 871 3423 Fragments arranged by size: 3423 2077 1770 871 273 131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7132 7506 7652 387 Size: 1064 138 5543 374 146 1280 Fragments arranged by size: 5543 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2011 2011 Size: 8545 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2186 2480 2560 2845 3088 3535 Size: 393 461 93 294 80 285 243 447 Cuts at: 3535 4312 4429 4547 4739 4945 5185 5435 5468 Size: 777 117 118 192 206 240 250 33 Cuts at: 5468 5543 5639 6608 7205 7992 8260 1239 Size: 75 96 969 597 787 268 1524 Fragments arranged by size: 1524 969 787 777 597 461 447 393 294 285 268 250 243 240 206 192 118 117 96 93 80 75 33 EcoNI CCTnn'n_nnAGG Cuts at: 2343 3932 2343 Size: 1589 6956 EcoO109I rG'GnC_Cy Cuts at: 2101 2478 2986 3290 4235 4719 2101 Size: 377 508 304 945 484 5927 Fragments arranged by size: 5927 945 508 484 377 304 EcoRI G'AATT_C Cuts at: 3098 5825 5854 6232 3098 Size: 2727 29 378 5411 Fragments arranged by size: 5411 2727 378 29 FspI TGC'GCA Cuts at: 7051 7051 Size: 8545 HaeII r_GCGC'y Cuts at: 1678 2045 2072 2451 2587 4087 5425 7692 1678 Size: 367 27 379 136 1500 1338 2267 2531 Fragments arranged by size: 2531 2267 1500 1338 379 367 136 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2481 2513 2663 Size: 32 135 32 458 32 419 32 150 Cuts at: 2663 2695 3700 3732 3941 3955 3973 3987 4090 Size: 32 1005 32 209 14 18 14 103 Cuts at: 4090 4103 4122 4135 4580 4612 7186 7218 7260 Size: 13 19 13 445 32 2574 32 42 Cuts at: 7260 7292 1373 Size: 32 2626 Fragments arranged by size: 2626 2574 1005 458 445 419 209 150 135 103 42 32 32 32 32 32 32 32 32 32 19 18 14 14 13 13 HincII GTy'rAC Cuts at: 669 1657 1938 3199 5889 6135 669 Size: 988 281 1261 2690 246 3079 Fragments arranged by size: 3079 2690 1261 988 281 246 HindIII A'AGCT_T Cuts at: 748 2624 3645 5801 748 Size: 1876 1021 2156 3492 Fragments arranged by size: 3492 2156 1876 1021 HpaI GTT'AAC Cuts at: 6135 6135 Size: 8545 KasI G'GCGC_C Cuts at: 2447 2447 Size: 8545 KpnI G_GTAC'C Cuts at: 2468 5870 2468 Size: 3402 5143 MfeI C'AATT_G Cuts at: 2935 6144 2935 Size: 3209 5336 MluI A'CGCG_T Cuts at: 8421 8421 Size: 8545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2103 2130 4520 7659 7843 1305 Size: 798 27 2390 3139 184 2007 Fragments arranged by size: 3139 2390 2007 798 184 27 MscI TGG'CCA Cuts at: 10 64 1489 1832 4001 4658 10 Size: 54 1425 343 2169 657 3897 Fragments arranged by size: 3897 2169 1425 657 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5129 6503 6862 7021 518 Size: 648 3963 1374 359 159 2042 Fragments arranged by size: 3963 2042 1374 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2036 2089 2865 4672 5841 5961 6638 7786 Size: 430 53 776 1807 1169 120 677 1148 Cuts at: 7786 8031 1606 Size: 245 2120 Fragments arranged by size: 2120 1807 1169 1148 776 677 430 245 120 53 NaeI GCC'GGC Cuts at: 5441 5441 Size: 8545 NarI GG'CG_CC Cuts at: 2448 2448 Size: 8545 NcoI C'CATG_G Cuts at: 513 1065 2004 2532 513 Size: 552 939 528 6526 Fragments arranged by size: 6526 939 552 528 NdeI CA'TA_TG Cuts at: 387 387 Size: 8545 NgoMIV G'CCGG_C Cuts at: 5439 5439 Size: 8545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8545 NotI GC'GGCC_GC Cuts at: 5992 5992 Size: 8545 NsiI A_TGCA'T Cuts at: 4597 4597 Size: 8545 NspI r_CATG'y Cuts at: 1132 1229 2149 4595 5128 5384 5764 5903 7448 Size: 97 920 2446 533 256 380 139 1545 Cuts at: 7448 1132 Size: 2229 Fragments arranged by size: 2446 2229 1545 920 533 380 256 139 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8545 PciI A'CATG_T Cuts at: 7444 7444 Size: 8545 PflMI CCAn_nnn'nTGG Cuts at: 1108 2126 1108 Size: 1018 7527 PfoI T'CCnGG_A Cuts at: 4563 4563 Size: 8545 PmeI GTTT'AAAC Cuts at: 5849 5849 Size: 8545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6614 6646 8127 8159 6614 Size: 32 1481 32 7000 Fragments arranged by size: 7000 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2478 2986 3290 2101 Size: 377 508 304 7356 Fragments arranged by size: 7356 508 377 304 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8545 PsiI TTA'TAA Cuts at: 6115 6115 Size: 8545 PstI C_TGCA'G Cuts at: 830 4368 5897 830 Size: 3538 1529 3478 Fragments arranged by size: 3538 3478 1529 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8545 PvuII CAG'CTG Cuts at: 2865 4672 5841 2865 Size: 1807 1169 5569 Fragments arranged by size: 5569 1807 1169 SacI G_AGCT'C Cuts at: 2013 2013 Size: 8545 SacII CC_GC'GG Cuts at: 2037 2037 Size: 8545 SalI G'TCGA_C Cuts at: 1936 5887 1936 Size: 3951 4594 SbfI CC_TGCA'GG Cuts at: 5897 5897 Size: 8545 ScaI AGT'ACT Cuts at: 3472 5020 6793 8505 3472 Size: 1548 1773 1712 3512 Fragments arranged by size: 3512 1773 1712 1548 SfcI C'TryA_G Cuts at: 826 1045 4364 4824 5893 7028 7709 7900 826 Size: 219 3319 460 1069 1135 681 191 1471 Fragments arranged by size: 3319 1471 1135 1069 681 460 219 191 SfoI GGC'GCC Cuts at: 2449 2449 Size: 8545 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8545 SgrAI Cr'CCGG_yG Cuts at: 1700 2138 1700 Size: 438 8107 SmaI CCC'GGG Cuts at: 1872 5872 1872 Size: 4000 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2842 3404 3593 4581 6649 7550 Size: 197 926 899 562 189 988 2068 901 Cuts at: 7550 7812 820 Size: 262 1553 Fragments arranged by size: 2068 1553 988 926 901 899 562 262 197 189 SnaBI TAC'GTA Cuts at: 493 5111 6336 493 Size: 4618 1225 2702 Fragments arranged by size: 4618 2702 1225 SpeI A'CTAG_T Cuts at: 152 152 Size: 8545 SphI G_CATG'C Cuts at: 1132 4595 5384 5764 5903 1132 Size: 3463 789 380 139 3774 Fragments arranged by size: 3774 3463 789 380 139 SspI AAT'ATT Cuts at: 5 52 3008 3076 4374 6469 5 Size: 47 2956 68 1298 2095 2081 Fragments arranged by size: 2956 2095 2081 1298 68 47 StuI AGG'CCT Cuts at: 1517 4820 1517 Size: 3303 5242 StyI C'CwwG_G Cuts at: 513 1065 2004 2532 2809 2982 3483 3981 5984 Size: 552 939 528 277 173 501 498 2003 Cuts at: 5984 513 Size: 3074 Fragments arranged by size: 3074 2003 939 552 528 501 498 277 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2236 2240 3283 4743 6572 6589 6742 Size: 1255 656 4 1043 1460 1829 17 153 Cuts at: 6742 6927 7372 325 Size: 185 445 1498 Fragments arranged by size: 1829 1498 1460 1255 1043 656 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2510 2666 3257 Size: 274 80 33 51 1443 533 156 591 Cuts at: 3257 3470 5018 6791 8503 96 Size: 213 1548 1773 1712 138 Fragments arranged by size: 1773 1712 1548 1443 591 533 274 213 156 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 8505 8537 2159 Size: 32 6314 32 2167 Fragments arranged by size: 6314 2167 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8545 XbaI T'CTAG_A Cuts at: 5365 5881 5365 Size: 516 8029 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3337 4651 5443 3337 Size: 1314 792 6439 Fragments arranged by size: 6439 1314 792 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8545 XmaI C'CCGG_G Cuts at: 1870 5870 1870 Size: 4000 4545 XmnI GAAnn'nnTTC Cuts at: 2904 2955 3008 6674 2904 Size: 51 53 3666 4775 Fragments arranged by size: 4775 3666 53 51 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6353 276 Size: 53 83 186 936 4819 2468 Fragments arranged by size: 4819 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbvCI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI