Restriction map for
pFN21AE4277
(Linear) (Six-base) MAPSORT of: KIEE4277.seq Check: 6949 from: 1 to: 3840
KIEE4277.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 483 3840
Size: 483 3357
Acc65I G'GTAC_C
Cuts at: 0 459 3840
Size: 459 3381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 840 1083 1530 2424 2734 3430 3634
Size: 555 285 243 447 894 310 696 204
Cuts at: 3634 3840
Size: 206
Fragments arranged by size:
894 696 555 447 310 285 243 206
204
AfeI AGC'GCT
Cuts at: 0 2080 3840
Size: 2080 1760
AflII C'TTAA_G
Cuts at: 0 837 3840
Size: 837 3003
AlwNI CAG_nnn'CTG
Cuts at: 0 1824 1992 2298 3492 3836 3840
Size: 1824 168 306 1194 344 4
Fragments arranged by size:
1824 1194 344 306 168 4
ApaLI G'TGCA_C
Cuts at: 0 1450 2696 3840
Size: 1450 1246 1144
Fragments arranged by size:
1450 1246 1144
ApoI r'AATT_y
Cuts at: 0 1093 1351 1360 3820 3840
Size: 1093 258 9 2460 20
Fragments arranged by size:
2460 1093 258 20 9
AvaI C'yCGr_G
Cuts at: 0 424 3062 3840
Size: 424 2638 778
Fragments arranged by size:
2638 778 424
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1591 1624 3840
Size: 1591 33 2216
Fragments arranged by size:
2216 1591 33
BamHI G'GATC_C
Cuts at: 0 152 1674 3840
Size: 152 1522 2166
Fragments arranged by size:
2166 1522 152
BanI G'GyrC_C
Cuts at: 0 442 459 2970 3058 3840
Size: 442 17 2511 88 782
Fragments arranged by size:
2511 782 442 88 17
BanII G_rGCy'C
Cuts at: 0 8 730 3208 3840
Size: 8 722 2478 632
Fragments arranged by size:
2478 722 632 8
BbeI G_GCGC'C
Cuts at: 0 446 3840
Size: 446 3394
BbsI GAAGACnn'nnnn_
Cuts at: 0 2381 2885 3549 3840
Size: 2381 504 664 291
Fragments arranged by size:
2381 664 504 291
BciVI GTATCCnnnnn_n'
Cuts at: 0 1253 1946 3840
Size: 1253 693 1894
Fragments arranged by size:
1894 1253 693
BfrBI ATG'CAT
Cuts at: 0 2590 3840
Size: 2590 1250
BglI GCCn_nnn'nGGC
Cuts at: 0 34 587 3145 3840
Size: 34 553 2558 695
Fragments arranged by size:
2558 695 553 34
BglII A'GATC_T
Cuts at: 0 2093 3840
Size: 2093 1747
BlpI GC'TnA_GC
Cuts at: 0 724 3840
Size: 724 3116
Bme1580I G_kGCm'C
Cuts at: 0 1454 2700 3063 3840
Size: 1454 1246 363 777
Fragments arranged by size:
1454 1246 777 363
BmrI ACTGGGnnnn_n'
Cuts at: 0 109 1633 1904 3026 3280 3840
Size: 109 1524 271 1122 254 560
Fragments arranged by size:
1524 1122 560 271 254 109
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 93 2970 3840
Size: 93 2877 870
Fragments arranged by size:
2877 870 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 181 475 2307 2542 2940 3180 3463
Size: 88 93 294 1832 235 398 240 283
Cuts at: 3463 3538 3840
Size: 75 302
Fragments arranged by size:
1832 398 302 294 283 240 235 93
88 75
Bpu10I CC'TnA_GC
Cuts at: 0 2842 3840
Size: 2842 998
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 1573 2561 3840
Size: 1420 153 988 1279
Fragments arranged by size:
1420 1279 988 153
BsaI GGTCTCn'nnnn_
Cuts at: 0 680 3840
Size: 680 3160
BsaAI yAC'GTr
Cuts at: 0 3106 3840
Size: 3106 734
BsaHI Gr'CG_yC
Cuts at: 0 443 3840
Size: 443 3397
BsaWI w'CCGG_w
Cuts at: 0 19 2623 3840
Size: 19 2604 1217
Fragments arranged by size:
2604 1217 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 55 154 184 262 292 3840
Size: 25 30 99 30 78 30 3548
Fragments arranged by size:
3548 99 78 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 38 402 1444 2340 2466 3544 3840
Size: 38 364 1042 896 126 1078 296
Fragments arranged by size:
1078 1042 896 364 296 126 38
BseYI C'CCAG_C
Cuts at: 0 185 1678 2724 2899 3840
Size: 185 1493 1046 175 941
Fragments arranged by size:
1493 1046 941 185 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2704 3178 3490 3840
Size: 2704 474 312 350
Fragments arranged by size:
2704 474 350 312
BsiEI CG_ry'CG
Cuts at: 0 2216 3840
Size: 2216 1624
BsiHKAI G_wGCw'C
Cuts at: 0 8 1454 2686 2700 3840
Size: 8 1446 1232 14 1140
Fragments arranged by size:
1446 1232 1140 14 8
BsmI GAATG_Cn'
Cuts at: 0 2035 2172 3840
Size: 2035 137 1668
Fragments arranged by size:
2035 1668 137
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2295 2547 3840
Size: 2295 252 1293
Fragments arranged by size:
2295 1293 252
Bsp1286I G_dGCh'C
Cuts at: 0 8 730 1454 2686 2700 3063 3208 3840
Size: 8 722 724 1232 14 363 145 632
Fragments arranged by size:
1232 724 722 632 363 145 14 8
BspEI T'CCGG_A
Cuts at: 0 19 3840
Size: 19 3821
BspHI T'CATG_A
Cuts at: 0 1614 2039 3840
Size: 1614 425 1801
Fragments arranged by size:
1801 1614 425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3240 3750 3840
Size: 3240 510 90
Fragments arranged by size:
3240 510 90
BsrBI CCG'CTC
Cuts at: 0 273 3534 3747 3840
Size: 273 3261 213 93
Fragments arranged by size:
3261 273 213 93
BsrDI GCAATG_nn'
Cuts at: 0 2810 3774 3840
Size: 2810 964 66
Fragments arranged by size:
2810 964 66
BsrFI r'CCGG_y
Cuts at: 0 133 3170 3434 3840
Size: 133 3037 264 406
Fragments arranged by size:
3037 406 264 133
BssHII G'CGCG_C
Cuts at: 0 3725 3840
Size: 3725 115
BssSI C'ACGA_G
Cuts at: 0 633 677 1781 2240 3840
Size: 633 44 1104 459 1600
Fragments arranged by size:
1600 1104 633 459 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2970 3483 3492 3840
Size: 2970 513 9 348
Fragments arranged by size:
2970 513 348 9
BstEII G'GTnAC_C
Cuts at: 0 828 3840
Size: 828 3012
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 528 2743 3840
Size: 453 75 2215 1097
Fragments arranged by size:
2215 1097 453 75
BstYI r'GATC_y
Cuts at: 0 152 753 1674 2093 3029 3840
Size: 152 601 921 419 936 811
Fragments arranged by size:
936 921 811 601 419 152
Bsu36I CC'TnA_GG
Cuts at: 0 385 3840
Size: 385 3455
BtgI C'CryG_G
Cuts at: 0 29 527 1830 2216 3840
Size: 29 498 1303 386 1624
Fragments arranged by size:
1624 1303 498 386 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 393 3840
Size: 393 3447
BtsI GCAGTG_nn'
Cuts at: 0 533 1202 1825 2846 3840
Size: 533 669 623 1021 994
Fragments arranged by size:
1021 994 669 623 533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2874 2909 3840
Size: 2874 35 931
Fragments arranged by size:
2874 931 35
DraIII CAC_nnn'GTG
Cuts at: 0 2970 3094 3840
Size: 2970 124 746
Fragments arranged by size:
2970 746 124
DrdI GACnn_nn'nnGTC
Cuts at: 0 109 3840
Size: 109 3731
EaeI y'GGCC_r
Cuts at: 0 46 1994 2651 3146 3840
Size: 46 1948 657 495 694
Fragments arranged by size:
1948 694 657 495 46
EarI CTCTTCn'nnn_
Cuts at: 0 1118 3195 3326 3599 3840
Size: 1118 2077 131 273 241
Fragments arranged by size:
2077 1118 273 241 131
EcoICRI GAG'CTC
Cuts at: 0 6 3840
Size: 6 3834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 181 475 555 840 1083 1530 2307
Size: 88 93 294 80 285 243 447 777
Cuts at: 2307 2424 2542 2734 2940 3180 3430 3463 3538
Size: 117 118 192 206 240 250 33 75
Cuts at: 3538 3634 3840
Size: 96 206
Fragments arranged by size:
777 447 294 285 250 243 240 206
206 192 118 117 96 93 88 80
75 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 338 1927 3840
Size: 338 1589 1913
Fragments arranged by size:
1913 1589 338
EcoO109I rG'GnC_Cy
Cuts at: 0 96 473 981 1285 2230 2714 3840
Size: 96 377 508 304 945 484 1126
Fragments arranged by size:
1126 945 508 484 377 304 96
EcoRI G'AATT_C
Cuts at: 0 1093 3820 3840
Size: 1093 2727 20
Fragments arranged by size:
2727 1093 20
HaeII r_GCGC'y
Cuts at: 0 40 67 446 582 2082 3420 3840
Size: 40 27 379 136 1500 1338 420
Fragments arranged by size:
1500 1338 420 379 136 40 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 476 508 658 690 1695 1727
Size: 25 32 419 32 150 32 1005 32
Cuts at: 1727 1936 1950 1968 1982 2085 2098 2117 2130
Size: 209 14 18 14 103 13 19 13
Cuts at: 2130 2575 2607 3840
Size: 445 32 1233
Fragments arranged by size:
1233 1005 445 419 209 150 103 32
32 32 32 32 25 19 18 14
14 13 13
HincII GTy'rAC
Cuts at: 0 1194 3840
Size: 1194 2646
HindIII A'AGCT_T
Cuts at: 0 619 1640 3796 3840
Size: 619 1021 2156 44
Fragments arranged by size:
2156 1021 619 44
KasI G'GCGC_C
Cuts at: 0 442 3840
Size: 442 3398
KpnI G_GTAC'C
Cuts at: 0 463 3840
Size: 463 3377
MfeI C'AATT_G
Cuts at: 0 930 3840
Size: 930 2910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 125 2515 3840
Size: 98 27 2390 1325
Fragments arranged by size:
2390 1325 98 27
MscI TGG'CCA
Cuts at: 0 1996 2653 3840
Size: 1996 657 1187
Fragments arranged by size:
1996 1187 657
MslI CAynn'nnrTG
Cuts at: 0 3124 3840
Size: 3124 716
MspA1I CmG'CkG
Cuts at: 0 31 84 860 2667 3836 3840
Size: 31 53 776 1807 1169 4
Fragments arranged by size:
1807 1169 776 53 31 4
NaeI GCC'GGC
Cuts at: 0 3436 3840
Size: 3436 404
NarI GG'CG_CC
Cuts at: 0 443 3840
Size: 443 3397
NcoI C'CATG_G
Cuts at: 0 527 3840
Size: 527 3313
NgoMIV G'CCGG_C
Cuts at: 0 3434 3840
Size: 3434 406
NsiI A_TGCA'T
Cuts at: 0 2592 3840
Size: 2592 1248
NspI r_CATG'y
Cuts at: 0 144 2590 3123 3379 3759 3840
Size: 144 2446 533 256 380 81
Fragments arranged by size:
2446 533 380 256 144 81
PflMI CCAn_nnn'nTGG
Cuts at: 0 121 3840
Size: 121 3719
PfoI T'CCnGG_A
Cuts at: 0 2558 3840
Size: 2558 1282
PpuMI rG'GwC_Cy
Cuts at: 0 96 473 981 1285 3840
Size: 96 377 508 304 2555
Fragments arranged by size:
2555 508 377 304 96
PstI C_TGCA'G
Cuts at: 0 2363 3840
Size: 2363 1477
PvuII CAG'CTG
Cuts at: 0 860 2667 3836 3840
Size: 860 1807 1169 4
Fragments arranged by size:
1807 1169 860 4
SacI G_AGCT'C
Cuts at: 0 8 3840
Size: 8 3832
SacII CC_GC'GG
Cuts at: 0 32 3840
Size: 32 3808
ScaI AGT'ACT
Cuts at: 0 1467 3015 3840
Size: 1467 1548 825
Fragments arranged by size:
1548 1467 825
SfcI C'TryA_G
Cuts at: 0 2359 2819 3840
Size: 2359 460 1021
Fragments arranged by size:
2359 1021 460
SfoI GGC'GCC
Cuts at: 0 444 3840
Size: 444 3396
SgrAI Cr'CCGG_yG
Cuts at: 0 133 3840
Size: 133 3707
SmlI C'TyrA_G
Cuts at: 0 837 1399 1588 2576 3840
Size: 837 562 189 988 1264
Fragments arranged by size:
1264 988 837 562 189
SnaBI TAC'GTA
Cuts at: 0 3106 3840
Size: 3106 734
SphI G_CATG'C
Cuts at: 0 2590 3379 3759 3840
Size: 2590 789 380 81
Fragments arranged by size:
2590 789 380 81
SspI AAT'ATT
Cuts at: 0 1003 1071 2369 3840
Size: 1003 68 1298 1471
Fragments arranged by size:
1471 1298 1003 68
StuI AGG'CCT
Cuts at: 0 2815 3840
Size: 2815 1025
StyI C'CwwG_G
Cuts at: 0 527 804 977 1478 1976 3840
Size: 527 277 173 501 498 1864
Fragments arranged by size:
1864 527 501 498 277 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 231 235 1278 2738 3840
Size: 231 4 1043 1460 1102
Fragments arranged by size:
1460 1102 1043 231 4
TatI w'GTAC_w
Cuts at: 0 505 661 1252 1465 3013 3840
Size: 505 156 591 213 1548 827
Fragments arranged by size:
1548 827 591 505 213 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 3840
Size: 154 32 3654
Fragments arranged by size:
3654 154 32
XbaI T'CTAG_A
Cuts at: 0 3360 3840
Size: 3360 480
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1332 2646 3438 3840
Size: 1332 1314 792 402
Fragments arranged by size:
1332 1314 792 402
XmnI GAAnn'nnTTC
Cuts at: 0 899 950 1003 3840
Size: 899 51 53 2837
Fragments arranged by size:
2837 899 53 51
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflII AlwNI ApaLI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PflMI PfoI PpuMI PstI PvuII SacI
SacII ScaI SfcI SfoI SgrAI SmlI SnaBI SphI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AclI AflIII AgeI AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvrII BbvCI BcgI
BclI BmgBI BmtI BsaBI BsiWI BsrGI BstBI BstZ17I
ClaI DraI EagI EciI EcoRV FalI FseI FspI
FspAI HpaI MluI NdeI NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
SexAI SfiI SgfI SmaI SpeI SrfI SwaI Tth111I
XhoI XmaI ZraI