################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BcgI BcnI BfrI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII Hin1I HindIII KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 779 GTCGAC GT^[AC][GT]AC AhdI 340 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 340 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 81 CCTCAGC CCTCAGC(-5/-2) BciVI 1080 GGATAC GTATCC(6/5) BfaI 361 CTAG C^TAG BglI 627 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 357 AGATCT A^GATCT BpmI 67 CTGGAG CTGGAG(16/14) BsaJI 1066 CCATGG C^C[ACGT][ACGT]GG BseRI 148 GAGGAG GAGGAG(10/8) BsgI 758 GTGCAG GTGCAG(16/14) BsiWI 133 CGTACG C^GTACG BsmAI 348 GTCTC GTCTC(1/5) BsmI 1013 GAATGC GAATGC(1/-1) Bsp1407I 195 TGTACA T^GTACA BsrDI 934 GCAATG GCAATG(2/0) BsrFI 812 GCCGGT [GA]^CCGG[CT] BsrGI 195 TGTACA T^GTACA BstXI 286 CCAATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 812 GCCGGT [GA]^CCGG[CT] DsaI 1066 CCATGG C^C[GA][CT]GG Eam1105I 340 GACTGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 1066 CCATGG C^C[AT][AT]GG FauI 4 GCGGG CCCGC(4/6) HapII 813 CCGG C^CGG HgaI 177 GACGC GACGC(5/10) HpaI 763 GTTAAC GTT^AAC HpaII 813 CCGG C^CGG MaeI 361 CTAG C^TAG MslI 751 CATACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 813 CCGG C^CGG NcoI 1066 CCATGG C^CATGG NlaIV 59 GGGGCC GG[ACGT]^[ACGT]CC PleI 659 GAGTC GAGTC(4/5) PvuII 617 CAGCTG CAG^CTG SalI 779 GTCGAC G^TCGAC SexAI 549 ACCAGGT A^CC[AT]GGT SfaNI 1044 GCATC GCATC(5/9) SfcI 426 CTATAG C^T[GA][CT]AG SmlI 995 CTCAAG C^T[CT][GA]AG SspBI 195 TGTACA T^GTACA StyI 1066 CCATGG C^C[AT][AT]GG TaqI 780 TCGA T^CGA TthHB8I 780 TCGA T^CGA XbaI 360 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 688 AAATTC [GA]^AATT[CT] 916 GAATTT ApoI 688 AAATTC [GA]^AATT[CT] 916 GAATTT Asp700I 93 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 452 GAAGAGCTTC AvaII 547 GGACC G^G[AT]CC 1074 GGTCC BclI 512 TGATCA T^GATCA 798 TGATCA BspHI 228 TCATGA T^CATGA 1035 TCATGA CfoI 18 GCGC GCG^C 130 GCGC EaeI 74 CGGCCA [CT]^GGCC[GA] 164 CGGCCA FbaI 512 TGATCA T^GATCA 798 TGATCA HhaI 18 GCGC GCG^C 130 GCGC HinP1I 18 GCGC G^CGC 130 GCGC MaeIII 391 GTCAC ^GT[ACGT]AC 490 GTCAC MseI 370 TTAA T^TAA 764 TTAA PflMI 613 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 946 CCAGCCTTTGG RcaI 228 TCATGA T^CATGA 1035 TCATGA SapI 452 GAAGAGC GCTCTTC(1/4) 745 GAAGAGC Tru9I 370 TTAA T^TAA 764 TTAA Tsp45I 391 GTCAC ^GT[GC]AC 490 GTCAC Van91I 613 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 946 CCAGCCTTTGG XmnI 93 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 452 GAAGAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI BbsI BbvI BpuAI BsiYI BslI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MboI MboII MflI MnlI MspA1I MvaI MvnI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI TfiI TseI Tsp509I TspRI XhoII