(Circular) (Six-base) MAPSORT of: pFN21AE1289.seq Check: 2451 from: 1 to: 7492 pFN21AE1289 7492 bp With 182 enzymes: SgfI * March 28, 2012 12:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2419 2419 Size: 7492 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5302 278 Size: 53 83 186 936 3766 2468 Fragments arranged by size: 3766 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2233 4835 1937 Size: 296 2602 4594 Fragments arranged by size: 4594 2602 296 Acc65I G'GTAC_C Cuts at: 4671 4813 4671 Size: 142 7350 AclI AA'CG_TT Cuts at: 1563 5619 5992 1563 Size: 4056 373 3063 Fragments arranged by size: 4056 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2577 2583 3021 3270 5555 6939 7207 Size: 677 268 6 438 249 2285 1384 268 Cuts at: 7207 1632 Size: 1917 Fragments arranged by size: 2285 1917 1384 677 438 268 268 249 6 AfeI AGC'GCT Cuts at: 2123 2123 Size: 7492 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7295 AflIII A'CryG_T Cuts at: 1203 6391 7368 1203 Size: 5188 977 1327 Fragments arranged by size: 5188 1327 977 AgeI A'CCGG_T Cuts at: 6323 6323 Size: 7492 AhdI GACnn_n'nnGTC Cuts at: 6221 6221 Size: 7492 AleI CACnn'nnGTG Cuts at: 1166 2111 4568 1166 Size: 945 2457 4090 Fragments arranged by size: 4090 2457 945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7492 AlwNI CAG_nnn'CTG Cuts at: 2065 2094 2137 5235 6807 2065 Size: 29 43 3098 1572 2750 Fragments arranged by size: 3098 2750 1572 43 29 ApaI G_GGCC'C Cuts at: 4478 4478 Size: 7492 ApaLI G'TGCA_C Cuts at: 5549 6705 5549 Size: 1156 6336 ApoI r'AATT_y Cuts at: 1493 2289 2524 3227 3279 3737 3785 4801 5027 Size: 796 235 703 52 458 48 1016 226 Cuts at: 5027 5179 7288 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1016 796 703 458 235 226 152 52 48 AseI AT'TA_AT Cuts at: 160 1032 2514 6046 160 Size: 872 1482 3532 1606 Fragments arranged by size: 3532 1606 1482 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7492 AvaI C'yCGr_G Cuts at: 1870 1943 2640 2735 4264 4346 4495 4817 1870 Size: 73 697 95 1529 82 149 322 4545 Fragments arranged by size: 4545 1529 697 322 149 95 82 73 BamHI G'GATC_C Cuts at: 3344 4822 5189 3344 Size: 1478 367 5647 Fragments arranged by size: 5647 1478 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2431 2722 4360 4671 4813 Size: 325 217 627 644 291 1638 311 142 Cuts at: 4813 6268 618 Size: 1455 1842 Fragments arranged by size: 1842 1638 1455 644 627 325 311 291 217 142 BanII G_rGCy'C Cuts at: 1391 2169 2957 2975 4478 4789 4811 1391 Size: 778 788 18 1503 311 22 4072 Fragments arranged by size: 4072 1503 788 778 311 22 18 BbeI G_GCGC'C Cuts at: 4364 4364 Size: 7492 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2253 2394 2855 4388 928 Size: 971 354 141 461 1533 4032 Fragments arranged by size: 4032 1533 971 461 354 141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5683 5717 1903 Size: 34 3746 34 3678 Fragments arranged by size: 3746 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2929 3735 5384 6594 2929 Size: 806 1649 1210 3827 Fragments arranged by size: 3827 1649 1210 806 BclI T'GATC_A Cuts at: 1548 1548 Size: 7492 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4349 4358 4514 Size: 107 122 71 631 605 2677 9 156 Cuts at: 4514 6103 136 Size: 1589 1525 Fragments arranged by size: 2677 1589 1525 631 605 156 122 107 71 9 BglII A'GATC_T Cuts at: 2052 4596 7487 2052 Size: 2544 2891 2057 Fragments arranged by size: 2891 2544 2057 BlpI GC'TnA_GC Cuts at: 2087 3204 4909 2087 Size: 1117 1705 4670 Fragments arranged by size: 4670 1705 1117 Bme1580I G_kGCm'C Cuts at: 1790 2436 4478 5553 6709 1790 Size: 646 2042 1075 1156 2573 Fragments arranged by size: 2573 2042 1156 1075 646 BmgBI CAC'GTC Cuts at: 1318 2113 1318 Size: 795 6697 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2866 3449 5223 Size: 416 53 83 399 24 1449 583 1774 Cuts at: 5223 6181 442 Size: 958 1753 Fragments arranged by size: 1774 1753 1449 958 583 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7492 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2035 2666 2035 Size: 631 6861 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2005 2676 2739 2823 4003 6152 1239 Size: 766 671 63 84 1180 2149 2579 Fragments arranged by size: 2579 2149 1180 766 671 84 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2391 3361 5617 6482 6780 2391 Size: 970 2256 865 298 3103 Fragments arranged by size: 3103 2256 970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2044 2533 3004 6155 882 Size: 617 545 489 471 3151 2219 Fragments arranged by size: 3151 2219 617 545 489 471 BsaAI yAC'GTr Cuts at: 493 1204 3428 4570 5283 493 Size: 711 2224 1142 713 2702 Fragments arranged by size: 2702 2224 1142 713 711 BsaBI GATnn'nnATC Cuts at: 3244 3798 4322 4601 7486 3244 Size: 554 524 279 2885 3250 Fragments arranged by size: 3250 2885 554 524 279 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4361 5299 5681 6316 Size: 53 83 186 936 2828 938 382 635 Cuts at: 6316 275 Size: 1451 Fragments arranged by size: 2828 1451 938 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3365 4060 5925 6323 6597 6744 7155 3365 Size: 695 1865 398 274 147 411 3702 Fragments arranged by size: 3702 1865 695 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2821 2851 1178 Size: 30 165 30 1418 30 5819 Fragments arranged by size: 5819 1418 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2093 2513 3342 3489 3548 4091 1186 Size: 907 420 829 147 59 543 4587 Fragments arranged by size: 4587 907 829 543 420 147 59 BseYI C'CCAG_C Cuts at: 2065 2070 2137 3107 3952 4027 6695 7191 2065 Size: 5 67 970 845 75 2668 496 2366 Fragments arranged by size: 2668 2366 970 845 496 75 67 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2102 4686 1163 Size: 573 366 2584 3969 Fragments arranged by size: 3969 2584 573 366 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4942 5703 6731 1540 Size: 275 186 2941 761 1028 2301 Fragments arranged by size: 2941 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2169 5553 5638 6709 2169 Size: 3384 85 1071 2952 Fragments arranged by size: 3384 2952 1071 85 BsmI GAATG_Cn' Cuts at: 5003 5096 5003 Size: 93 7399 BsmBI CGTCTCn'nnnn_ Cuts at: 4547 4547 Size: 7492 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2436 2957 2975 4478 4789 4811 Size: 399 379 267 521 18 1503 311 22 Cuts at: 4811 5553 5638 6709 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1503 1071 742 521 399 379 311 267 85 22 18 BspEI T'CCGG_A Cuts at: 4060 7155 4060 Size: 3095 4397 BspHI T'CATG_A Cuts at: 4695 5381 7111 4695 Size: 686 1730 5076 Fragments arranged by size: 5076 1730 686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2394 2419 2458 4847 844 Size: 1550 25 39 2389 3489 Fragments arranged by size: 3489 2389 1550 39 25 BsrBI CCG'CTC Cuts at: 1394 1823 5379 1394 Size: 429 3556 3507 Fragments arranged by size: 3556 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2889 5987 6161 65 Size: 1183 1641 3098 174 1396 Fragments arranged by size: 3098 1641 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4357 4393 6136 6323 1700 Size: 110 2547 36 1743 187 2869 Fragments arranged by size: 2869 2547 1743 187 110 36 BsrGI T'GTAC_A Cuts at: 96 2701 96 Size: 2605 4887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7492 BssSI C'ACGA_G Cuts at: 3192 5552 6564 3192 Size: 2360 1012 4120 Fragments arranged by size: 4120 2360 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7492 BstBI TT'CG_AA Cuts at: 3225 6296 3225 Size: 3071 4421 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7492 BstYI r'GATC_y Cuts at: 1972 2052 2673 3217 3344 3760 4596 4602 4822 Size: 80 621 544 127 416 836 6 220 Cuts at: 4822 5189 5574 5591 7043 7129 7141 7487 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 836 621 544 416 385 367 346 220 127 86 80 17 12 6 BtgI C'CryG_G Cuts at: 513 1065 3558 4470 513 Size: 552 2493 912 3535 Fragments arranged by size: 3535 2493 912 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4677 487 Size: 683 56 3451 3302 Fragments arranged by size: 3451 3302 683 56 BtsI GCAGTG_nn' Cuts at: 4727 5008 5802 5822 4727 Size: 281 794 20 6397 Fragments arranged by size: 6397 794 281 20 ClaI AT'CG_AT Cuts at: 1554 3177 1554 Size: 1623 5869 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3951 3986 529 Size: 35 3387 35 4035 Fragments arranged by size: 4035 3387 35 35 DraI TTT'AAA Cuts at: 3775 4796 3775 Size: 1021 6471 DraIII CAC_nnn'GTG Cuts at: 3050 3526 3050 Size: 476 7016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4319 6499 809 Size: 507 3003 2180 1802 Fragments arranged by size: 3003 2180 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2173 4306 4939 Size: 54 1425 50 275 18 343 2133 633 Cuts at: 4939 5828 7457 8 Size: 889 1629 43 Fragments arranged by size: 2133 1629 1425 889 633 343 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4939 1537 Size: 275 3127 4090 Fragments arranged by size: 4090 3127 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2418 3487 3747 4178 4193 4707 4766 5422 Size: 1065 1069 260 431 15 514 59 656 Cuts at: 5422 1353 Size: 3423 Fragments arranged by size: 3423 1069 1065 656 514 431 260 59 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6079 6453 6599 387 Size: 1064 138 4490 374 146 1280 Fragments arranged by size: 4490 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2167 2167 Size: 7492 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2005 2309 2577 2583 2676 2739 2823 Size: 393 373 304 268 6 93 63 84 Cuts at: 2823 3021 3270 4003 5555 6152 6939 7207 1239 Size: 198 249 733 1552 597 787 268 1524 Fragments arranged by size: 1552 1524 787 733 597 393 373 304 268 268 249 198 93 84 63 6 EcoO109I rG'GnC_Cy Cuts at: 4024 4475 4499 4024 Size: 451 24 7017 Fragments arranged by size: 7017 451 24 EcoRI G'AATT_C Cuts at: 4801 5179 4801 Size: 378 7114 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3842 3842 Size: 7492 FspI TGC'GCA Cuts at: 4538 5998 4538 Size: 1460 6032 HaeII r_GCGC'y Cuts at: 1678 2066 2094 2125 4364 6639 1678 Size: 388 28 31 2239 2275 2531 Fragments arranged by size: 2531 2275 2239 388 31 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4275 4307 4765 4797 6133 Size: 32 135 32 2703 32 458 32 1336 Cuts at: 6133 6165 6207 6239 1373 Size: 32 42 32 2626 Fragments arranged by size: 2703 2626 1336 458 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4836 5082 669 Size: 988 281 2898 246 3079 Fragments arranged by size: 3079 2898 988 281 246 HindIII A'AGCT_T Cuts at: 748 2208 3309 748 Size: 1460 1101 4931 Fragments arranged by size: 4931 1460 1101 HpaI GTT'AAC Cuts at: 5082 5082 Size: 7492 KasI G'GCGC_C Cuts at: 4360 4360 Size: 7492 KpnI G_GTAC'C Cuts at: 4675 4817 4675 Size: 142 7350 MfeI C'AATT_G Cuts at: 5091 5091 Size: 7492 MluI A'CGCG_T Cuts at: 7368 7368 Size: 7492 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2869 4669 6606 6790 1305 Size: 1564 1800 1937 184 2007 Fragments arranged by size: 2007 1937 1800 1564 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4308 10 Size: 54 1425 343 2476 3194 Fragments arranged by size: 3194 2476 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 4227 4568 5450 5809 5968 518 Size: 648 945 2116 341 882 359 159 2042 Fragments arranged by size: 2116 2042 945 882 648 359 341 159 MspA1I CmG'CkG Cuts at: 1606 2137 2218 4447 4453 4472 4908 5585 6733 Size: 531 81 2229 6 19 436 677 1148 Cuts at: 6733 6978 1606 Size: 245 2120 Fragments arranged by size: 2229 2120 1148 677 531 436 245 81 19 6 NaeI GCC'GGC Cuts at: 4359 4395 4359 Size: 36 7456 NarI GG'CG_CC Cuts at: 4361 4361 Size: 7492 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6940 NdeI CA'TA_TG Cuts at: 387 387 Size: 7492 NgoMIV G'CCGG_C Cuts at: 4357 4393 4357 Size: 36 7456 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7492 NotI GC'GGCC_GC Cuts at: 4939 4939 Size: 7492 NspI r_CATG'y Cuts at: 1132 1229 4850 6395 1132 Size: 97 3621 1545 2229 Fragments arranged by size: 3621 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4610 1348 Size: 3262 4230 PciI A'CATG_T Cuts at: 6391 6391 Size: 7492 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 2221 1108 Size: 957 156 6379 Fragments arranged by size: 6379 957 156 PfoI T'CCnGG_A Cuts at: 2839 2839 Size: 7492 PmeI GTTT'AAAC Cuts at: 4796 4796 Size: 7492 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5561 5593 7074 7106 5561 Size: 32 1481 32 5947 Fragments arranged by size: 5947 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4024 4499 4024 Size: 475 7017 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7492 PsiI TTA'TAA Cuts at: 5062 5062 Size: 7492 PspOMI G'GGCC_C Cuts at: 4474 4474 Size: 7492 PstI C_TGCA'G Cuts at: 830 2121 2133 4844 830 Size: 1291 12 2711 3478 Fragments arranged by size: 3478 2711 1291 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7492 PvuII CAG'CTG Cuts at: 2137 2218 2137 Size: 81 7411 SacI G_AGCT'C Cuts at: 2169 2169 Size: 7492 SacII CC_GC'GG Cuts at: 4473 4473 Size: 7492 SalI G'TCGA_C Cuts at: 1936 4834 1936 Size: 2898 4594 SanDI GG'GwC_CC Cuts at: 4024 4499 4024 Size: 475 7017 SapI GCTCTTCn'nnn_ Cuts at: 2418 2418 Size: 7492 SbfI CC_TGCA'GG Cuts at: 4844 4844 Size: 7492 ScaI AGT'ACT Cuts at: 5740 7452 5740 Size: 1712 5780 SfcI C'TryA_G Cuts at: 826 1045 2117 2129 4840 5975 6656 6847 826 Size: 219 1072 12 2711 1135 681 191 1471 Fragments arranged by size: 2711 1471 1135 1072 681 219 191 12 SfoI GGC'GCC Cuts at: 4362 4362 Size: 7492 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7492 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7492 SmaI CCC'GGG Cuts at: 1872 4497 4819 1872 Size: 2625 322 4545 Fragments arranged by size: 4545 2625 322 SmlI C'TyrA_G Cuts at: 820 1017 1943 2406 2640 3376 5596 6497 6759 Size: 197 926 463 234 736 2220 901 262 Cuts at: 6759 820 Size: 1553 Fragments arranged by size: 2220 1553 926 901 736 463 262 234 197 SnaBI TAC'GTA Cuts at: 493 3428 5283 493 Size: 2935 1855 2702 Fragments arranged by size: 2935 2702 1855 SpeI A'CTAG_T Cuts at: 152 152 Size: 7492 SphI G_CATG'C Cuts at: 1132 4850 1132 Size: 3718 3774 SspI AAT'ATT Cuts at: 5 52 5416 5 Size: 47 5364 2081 Fragments arranged by size: 5364 2081 47 StuI AGG'CCT Cuts at: 1517 3170 1517 Size: 1653 5839 StyI C'CwwG_G Cuts at: 513 1065 2475 2976 4931 513 Size: 552 1410 501 1955 3074 Fragments arranged by size: 3074 1955 1410 552 501 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2407 4602 4638 5519 5536 5689 5874 Size: 1255 827 2195 36 881 17 153 185 Cuts at: 5874 6319 325 Size: 445 1498 Fragments arranged by size: 2195 1498 1255 881 827 445 185 153 36 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2320 2701 2711 Size: 274 80 33 51 1443 343 381 10 Cuts at: 2711 3296 5738 7450 96 Size: 585 2442 1712 138 Fragments arranged by size: 2442 1712 1443 585 381 343 274 138 80 51 33 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7452 7484 7452 Size: 32 7460 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7492 XbaI T'CTAG_A Cuts at: 4828 4828 Size: 7492 XhoI C'TCGA_G Cuts at: 1943 2640 1943 Size: 697 6795 XmaI C'CCGG_G Cuts at: 1870 4495 4817 1870 Size: 2625 322 4545 Fragments arranged by size: 4545 2625 322 XmnI GAAnn'nnTTC Cuts at: 2373 3329 3448 5621 2373 Size: 956 119 2173 4244 Fragments arranged by size: 4244 2173 956 119 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5300 276 Size: 53 83 186 936 3766 2468 Fragments arranged by size: 3766 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI XcmI