(Linear) (Six-base) MAPSORT of: KIEE1289.seq Check: 3368 from: 1 to: 2787 KIEE1289.orf With 182 enzymes: SgfI * March 22, 2012 15:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 414 2787 Size: 414 2373 AccI GT'mk_AC Cuts at: 0 228 2787 Size: 228 2559 Acc65I G'GTAC_C Cuts at: 0 2666 2787 Size: 2666 121 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 572 578 1016 1265 2787 Size: 304 268 6 438 249 1522 Fragments arranged by size: 1522 438 304 268 249 6 AfeI AGC'GCT Cuts at: 0 118 2787 Size: 118 2669 AleI CACnn'nnGTG Cuts at: 0 106 2563 2787 Size: 106 2457 224 Fragments arranged by size: 2457 224 106 AlwNI CAG_nnn'CTG Cuts at: 0 60 89 132 2787 Size: 60 29 43 2655 Fragments arranged by size: 2655 60 43 29 ApaI G_GGCC'C Cuts at: 0 2473 2787 Size: 2473 314 ApoI r'AATT_y Cuts at: 0 284 519 1222 1274 1732 1780 2787 Size: 284 235 703 52 458 48 1007 Fragments arranged by size: 1007 703 458 284 235 52 48 AseI AT'TA_AT Cuts at: 0 509 2787 Size: 509 2278 AvaI C'yCGr_G Cuts at: 0 635 730 2259 2341 2490 2787 Size: 635 95 1529 82 149 297 Fragments arranged by size: 1529 635 297 149 95 82 BamHI G'GATC_C Cuts at: 0 1339 2787 Size: 1339 1448 BanI G'GyrC_C Cuts at: 0 426 717 2355 2666 2787 Size: 426 291 1638 311 121 Fragments arranged by size: 1638 426 311 291 121 BanII G_rGCy'C Cuts at: 0 164 952 970 2473 2784 2787 Size: 164 788 18 1503 311 3 Fragments arranged by size: 1503 788 311 164 18 3 BbeI G_GCGC'C Cuts at: 0 2359 2787 Size: 2359 428 BbsI GAAGACnn'nnnn_ Cuts at: 0 248 389 850 2383 2787 Size: 248 141 461 1533 404 Fragments arranged by size: 1533 461 404 248 141 BciVI GTATCCnnnnn_n' Cuts at: 0 924 1730 2787 Size: 924 806 1057 Fragments arranged by size: 1057 924 806 BglI GCCn_nnn'nGGC Cuts at: 0 2344 2353 2509 2787 Size: 2344 9 156 278 Fragments arranged by size: 2344 278 156 9 BglII A'GATC_T Cuts at: 0 47 2591 2787 Size: 47 2544 196 Fragments arranged by size: 2544 196 47 BlpI GC'TnA_GC Cuts at: 0 82 1199 2787 Size: 82 1117 1588 Fragments arranged by size: 1588 1117 82 Bme1580I G_kGCm'C Cuts at: 0 431 2473 2787 Size: 431 2042 314 Fragments arranged by size: 2042 431 314 BmgBI CAC'GTC Cuts at: 0 108 2787 Size: 108 2679 BmrI ACTGGGnnnn_n' Cuts at: 0 861 1444 2787 Size: 861 583 1343 Fragments arranged by size: 1343 861 583 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 30 661 2787 Size: 30 631 2126 Fragments arranged by size: 2126 631 30 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 671 734 818 1998 2787 Size: 671 63 84 1180 789 Fragments arranged by size: 1180 789 671 84 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 386 1356 2787 Size: 386 970 1431 Fragments arranged by size: 1431 970 386 BsaI GGTCTCn'nnnn_ Cuts at: 0 39 528 999 2787 Size: 39 489 471 1788 Fragments arranged by size: 1788 489 471 39 BsaAI yAC'GTr Cuts at: 0 1423 2565 2787 Size: 1423 1142 222 Fragments arranged by size: 1423 1142 222 BsaBI GATnn'nnATC Cuts at: 0 1239 1793 2317 2596 2787 Size: 1239 554 524 279 191 Fragments arranged by size: 1239 554 524 279 191 BsaHI Gr'CG_yC Cuts at: 0 2356 2787 Size: 2356 431 BsaWI w'CCGG_w Cuts at: 0 1360 2055 2787 Size: 1360 695 732 Fragments arranged by size: 1360 732 695 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 816 846 2787 Size: 816 30 1941 Fragments arranged by size: 1941 816 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 88 508 1337 1484 1543 2086 2787 Size: 88 420 829 147 59 543 701 Fragments arranged by size: 829 701 543 420 147 88 59 BseYI C'CCAG_C Cuts at: 0 60 65 132 1102 1947 2022 2787 Size: 60 5 67 970 845 75 765 Fragments arranged by size: 970 845 765 75 67 60 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 2681 2787 Size: 97 2584 106 Fragments arranged by size: 2584 106 97 BsiHKAI G_wGCw'C Cuts at: 0 164 2787 Size: 164 2623 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2542 2787 Size: 2542 245 Bsp1286I G_dGCh'C Cuts at: 0 164 431 952 970 2473 2784 2787 Size: 164 267 521 18 1503 311 3 Fragments arranged by size: 1503 521 311 267 164 18 3 BspEI T'CCGG_A Cuts at: 0 2055 2787 Size: 2055 732 BspHI T'CATG_A Cuts at: 0 2690 2787 Size: 2690 97 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 389 414 453 2787 Size: 389 25 39 2334 Fragments arranged by size: 2334 389 39 25 BsrDI GCAATG_nn' Cuts at: 0 884 2787 Size: 884 1903 BsrFI r'CCGG_y Cuts at: 0 2352 2388 2787 Size: 2352 36 399 Fragments arranged by size: 2352 399 36 BsrGI T'GTAC_A Cuts at: 0 696 2787 Size: 696 2091 BssSI C'ACGA_G Cuts at: 0 1187 2787 Size: 1187 1600 BstBI TT'CG_AA Cuts at: 0 1220 2787 Size: 1220 1567 BstYI r'GATC_y Cuts at: 0 47 668 1212 1339 1755 2591 2597 2787 Size: 47 621 544 127 416 836 6 190 Fragments arranged by size: 836 621 544 416 190 127 47 6 BtgI C'CryG_G Cuts at: 0 1553 2465 2787 Size: 1553 912 322 Fragments arranged by size: 1553 912 322 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2672 2787 Size: 2672 115 BtsI GCAGTG_nn' Cuts at: 0 2722 2787 Size: 2722 65 ClaI AT'CG_AT Cuts at: 0 1172 2787 Size: 1172 1615 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1946 1981 2787 Size: 1946 35 806 Fragments arranged by size: 1946 806 35 DraI TTT'AAA Cuts at: 0 1770 2787 Size: 1770 1017 DraIII CAC_nnn'GTG Cuts at: 0 1045 1521 2787 Size: 1045 476 1266 Fragments arranged by size: 1266 1045 476 DrdI GACnn_nn'nnGTC Cuts at: 0 2314 2787 Size: 2314 473 EaeI y'GGCC_r Cuts at: 0 168 2301 2787 Size: 168 2133 486 Fragments arranged by size: 2133 486 168 EarI CTCTTCn'nnn_ Cuts at: 0 413 1482 1742 2173 2188 2702 2761 2787 Size: 413 1069 260 431 15 514 59 26 Fragments arranged by size: 1069 514 431 413 260 59 26 15 EcoICRI GAG'CTC Cuts at: 0 162 2787 Size: 162 2625 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 572 578 671 734 818 1016 1265 Size: 304 268 6 93 63 84 198 249 Cuts at: 1265 1998 2787 Size: 733 789 Fragments arranged by size: 789 733 304 268 249 198 93 84 63 6 EcoO109I rG'GnC_Cy Cuts at: 0 2019 2470 2494 2787 Size: 2019 451 24 293 Fragments arranged by size: 2019 451 293 24 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1837 2787 Size: 1837 950 FspI TGC'GCA Cuts at: 0 2533 2787 Size: 2533 254 HaeII r_GCGC'y Cuts at: 0 61 89 120 2359 2787 Size: 61 28 31 2239 428 Fragments arranged by size: 2239 428 61 31 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 2270 2302 2760 2787 Size: 2270 32 458 27 Fragments arranged by size: 2270 458 32 27 HindIII A'AGCT_T Cuts at: 0 203 1304 2787 Size: 203 1101 1483 Fragments arranged by size: 1483 1101 203 KasI G'GCGC_C Cuts at: 0 2355 2787 Size: 2355 432 KpnI G_GTAC'C Cuts at: 0 2670 2787 Size: 2670 117 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 864 2664 2787 Size: 864 1800 123 Fragments arranged by size: 1800 864 123 MscI TGG'CCA Cuts at: 0 2303 2787 Size: 2303 484 MslI CAynn'nnrTG Cuts at: 0 106 2222 2563 2787 Size: 106 2116 341 224 Fragments arranged by size: 2116 341 224 106 MspA1I CmG'CkG Cuts at: 0 132 213 2442 2448 2467 2787 Size: 132 81 2229 6 19 320 Fragments arranged by size: 2229 320 132 81 19 6 NaeI GCC'GGC Cuts at: 0 2354 2390 2787 Size: 2354 36 397 Fragments arranged by size: 2354 397 36 NarI GG'CG_CC Cuts at: 0 2356 2787 Size: 2356 431 NgoMIV G'CCGG_C Cuts at: 0 2352 2388 2787 Size: 2352 36 399 Fragments arranged by size: 2352 399 36 PasI CC'CwG_GG Cuts at: 0 2605 2787 Size: 2605 182 PflMI CCAn_nnn'nTGG Cuts at: 0 60 216 2787 Size: 60 156 2571 Fragments arranged by size: 2571 156 60 PfoI T'CCnGG_A Cuts at: 0 834 2787 Size: 834 1953 PpuMI rG'GwC_Cy Cuts at: 0 2019 2494 2787 Size: 2019 475 293 Fragments arranged by size: 2019 475 293 PspOMI G'GGCC_C Cuts at: 0 2469 2787 Size: 2469 318 PstI C_TGCA'G Cuts at: 0 116 128 2787 Size: 116 12 2659 Fragments arranged by size: 2659 116 12 PvuII CAG'CTG Cuts at: 0 132 213 2787 Size: 132 81 2574 Fragments arranged by size: 2574 132 81 SacI G_AGCT'C Cuts at: 0 164 2787 Size: 164 2623 SacII CC_GC'GG Cuts at: 0 2468 2787 Size: 2468 319 SanDI GG'GwC_CC Cuts at: 0 2019 2494 2787 Size: 2019 475 293 Fragments arranged by size: 2019 475 293 SapI GCTCTTCn'nnn_ Cuts at: 0 413 2787 Size: 413 2374 SfcI C'TryA_G Cuts at: 0 112 124 2787 Size: 112 12 2663 Fragments arranged by size: 2663 112 12 SfoI GGC'GCC Cuts at: 0 2357 2787 Size: 2357 430 SmaI CCC'GGG Cuts at: 0 2492 2787 Size: 2492 295 SmlI C'TyrA_G Cuts at: 0 401 635 1371 2787 Size: 401 234 736 1416 Fragments arranged by size: 1416 736 401 234 SnaBI TAC'GTA Cuts at: 0 1423 2787 Size: 1423 1364 StuI AGG'CCT Cuts at: 0 1165 2787 Size: 1165 1622 StyI C'CwwG_G Cuts at: 0 470 971 2787 Size: 470 501 1816 Fragments arranged by size: 1816 501 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 402 2597 2633 2787 Size: 402 2195 36 154 Fragments arranged by size: 2195 402 154 36 TatI w'GTAC_w Cuts at: 0 315 696 706 1291 2787 Size: 315 381 10 585 1496 Fragments arranged by size: 1496 585 381 315 10 XhoI C'TCGA_G Cuts at: 0 635 2787 Size: 635 2152 XmaI C'CCGG_G Cuts at: 0 2490 2787 Size: 2490 297 XmnI GAAnn'nnTTC Cuts at: 0 368 1324 1443 2787 Size: 368 956 119 1344 Fragments arranged by size: 1344 956 368 119 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AleI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SfcI SfoI SmaI SmlI SnaBI StuI StyI TaqII TatI XhoI XmaI XmnI Enzymes that do not cut: AatII AclI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI BmtI Bpu10I BsiEI BsiWI BsmI BsrBI BssHII BstAPI BstEII BstXI BstZ17I Bsu36I EagI EciI EcoNI EcoRI EcoRV FseI FspAI HincII HpaI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI ZraI