(Linear) (Six-base) MAPSORT of: KIBB7746.seq Check: 2564 from: 1 to: 1461 KIBB7746.orf With 182 enzymes: SgfI * March 22, 2012 15:40 .. AccI GT'mk_AC Cuts at: 0 131 1461 Size: 131 1330 AclI AA'CG_TT Cuts at: 0 358 1461 Size: 358 1103 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 674 1250 1315 1461 Size: 674 576 65 146 Fragments arranged by size: 674 576 146 65 AflII C'TTAA_G Cuts at: 0 400 1306 1461 Size: 400 906 155 Fragments arranged by size: 906 400 155 AflIII A'CryG_T Cuts at: 0 1076 1461 Size: 1076 385 AhdI GACnn_n'nnGTC Cuts at: 0 621 1461 Size: 621 840 AleI CACnn'nnGTG Cuts at: 0 1102 1461 Size: 1102 359 AlwNI CAG_nnn'CTG Cuts at: 0 825 1461 Size: 825 636 ApoI r'AATT_y Cuts at: 0 1260 1461 Size: 1260 201 AseI AT'TA_AT Cuts at: 0 881 1461 Size: 881 580 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 591 624 1461 Size: 591 33 837 Fragments arranged by size: 837 591 33 BanI G'GyrC_C Cuts at: 0 1096 1461 Size: 1096 365 BanII G_rGCy'C Cuts at: 0 796 1461 Size: 796 665 BbvCI CC'TCA_GC Cuts at: 0 16 1461 Size: 16 1445 BciVI GTATCCnnnnn_n' Cuts at: 0 592 1461 Size: 592 869 BclI T'GATC_A Cuts at: 0 1106 1461 Size: 1106 355 BglI GCCn_nnn'nGGC Cuts at: 0 33 1432 1461 Size: 33 1399 29 Fragments arranged by size: 1399 33 29 Bme1580I G_kGCm'C Cuts at: 0 1018 1099 1461 Size: 1018 81 362 Fragments arranged by size: 1018 362 81 BmrI ACTGGGnnnn_n' Cuts at: 0 1102 1461 Size: 1102 359 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 560 1461 Size: 184 376 901 Fragments arranged by size: 901 376 184 Bpu10I CC'TnA_GC Cuts at: 0 16 797 1461 Size: 16 781 664 Fragments arranged by size: 781 664 16 BsaI GGTCTCn'nnnn_ Cuts at: 0 145 1150 1461 Size: 145 1005 311 Fragments arranged by size: 1005 311 145 BsaBI GATnn'nnATC Cuts at: 0 680 1461 Size: 680 781 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 702 732 1461 Size: 702 30 729 Fragments arranged by size: 729 702 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 710 1461 Size: 710 751 BseYI C'CCAG_C Cuts at: 0 920 1461 Size: 920 541 BsiHKAI G_wGCw'C Cuts at: 0 1070 1461 Size: 1070 391 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1437 1461 Size: 1437 24 Bsp1286I G_dGCh'C Cuts at: 0 796 1018 1070 1099 1461 Size: 796 222 52 29 362 Fragments arranged by size: 796 362 222 52 29 BspHI T'CATG_A Cuts at: 0 701 1461 Size: 701 760 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 502 1461 Size: 502 959 BsrDI GCAATG_nn' Cuts at: 0 241 1461 Size: 241 1220 BsrFI r'CCGG_y Cuts at: 0 32 154 1461 Size: 32 122 1307 Fragments arranged by size: 1307 122 32 BstAPI GCAn_nnn'nTGC Cuts at: 0 493 1461 Size: 493 968 BstXI CCAn_nnnn'nTGG Cuts at: 0 648 746 1461 Size: 648 98 715 Fragments arranged by size: 715 648 98 BstYI r'GATC_y Cuts at: 0 1382 1461 Size: 1382 79 BstZ17I GTA'TAC Cuts at: 0 132 1461 Size: 132 1329 BtgI C'CryG_G Cuts at: 0 530 890 1100 1461 Size: 530 360 210 361 Fragments arranged by size: 530 361 360 210 BtsI GCAGTG_nn' Cuts at: 0 1004 1461 Size: 1004 457 EaeI y'GGCC_r Cuts at: 0 156 533 1461 Size: 156 377 928 Fragments arranged by size: 928 377 156 EarI CTCTTCn'nnn_ Cuts at: 0 956 1121 1330 1373 1435 1461 Size: 956 165 209 43 62 26 Fragments arranged by size: 956 209 165 62 43 26 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 560 674 1250 1315 1461 Size: 184 376 114 576 65 146 Fragments arranged by size: 576 376 184 146 114 65 EcoO109I rG'GnC_Cy Cuts at: 0 116 1326 1461 Size: 116 1210 135 Fragments arranged by size: 1210 135 116 EcoRV GAT'ATC Cuts at: 0 336 447 678 1461 Size: 336 111 231 783 Fragments arranged by size: 783 336 231 111 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1359 1461 Size: 1359 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 607 639 733 765 1461 Size: 607 32 94 32 696 Fragments arranged by size: 696 607 94 32 32 HincII GTy'rAC Cuts at: 0 362 517 1461 Size: 362 155 944 Fragments arranged by size: 944 362 155 HpaI GTT'AAC Cuts at: 0 362 1461 Size: 362 1099 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 29 1294 1461 Size: 29 1265 167 Fragments arranged by size: 1265 167 29 MscI TGG'CCA Cuts at: 0 535 1461 Size: 535 926 MslI CAynn'nnrTG Cuts at: 0 646 895 1102 1461 Size: 646 249 207 359 Fragments arranged by size: 646 359 249 207 MspA1I CmG'CkG Cuts at: 0 15 39 927 1461 Size: 15 24 888 534 Fragments arranged by size: 888 534 24 15 NaeI GCC'GGC Cuts at: 0 34 1461 Size: 34 1427 NcoI C'CATG_G Cuts at: 0 530 890 1100 1461 Size: 530 360 210 361 Fragments arranged by size: 530 361 360 210 NgoMIV G'CCGG_C Cuts at: 0 32 1461 Size: 32 1429 NspI r_CATG'y Cuts at: 0 487 1461 Size: 487 974 PacI TTA_AT'TAA Cuts at: 0 881 1461 Size: 881 580 PasI CC'CwG_GG Cuts at: 0 627 1461 Size: 627 834 PflMI CCAn_nnn'nTGG Cuts at: 0 1288 1461 Size: 1288 173 PfoI T'CCnGG_A Cuts at: 0 200 1418 1461 Size: 200 1218 43 Fragments arranged by size: 1218 200 43 PpuMI rG'GwC_Cy Cuts at: 0 116 1326 1461 Size: 116 1210 135 Fragments arranged by size: 1210 135 116 PstI C_TGCA'G Cuts at: 0 830 1461 Size: 830 631 PvuII CAG'CTG Cuts at: 0 15 39 1461 Size: 15 24 1422 Fragments arranged by size: 1422 24 15 ScaI AGT'ACT Cuts at: 0 310 1461 Size: 310 1151 SexAI A'CCwGG_T Cuts at: 0 1245 1461 Size: 1245 216 SfcI C'TryA_G Cuts at: 0 826 1461 Size: 826 635 SmlI C'TyrA_G Cuts at: 0 400 1306 1461 Size: 400 906 155 Fragments arranged by size: 906 400 155 SspI AAT'ATT Cuts at: 0 262 386 462 1192 1461 Size: 262 124 76 730 269 Fragments arranged by size: 730 269 262 124 76 StyI C'CwwG_G Cuts at: 0 530 890 1100 1461 Size: 530 360 210 361 Fragments arranged by size: 530 361 360 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 848 991 1461 Size: 848 143 470 Fragments arranged by size: 848 470 143 TatI w'GTAC_w Cuts at: 0 308 1461 Size: 308 1153 XmnI GAAnn'nnTTC Cuts at: 0 416 584 1461 Size: 416 168 877 Fragments arranged by size: 877 416 168 Enzymes that do cut: AccI AclI AcuI AflII AflIII AhdI AleI AlwNI ApoI AseI BaeI BanI BanII BbvCI BciVI BclI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI BstZ17I BtgI BtsI EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I HincII HpaI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI PacI PasI PflMI PfoI PpuMI PstI PvuII ScaI SexAI SfcI SmlI SspI StyI TaqII TatI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AgeI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BbeI BbsI BcgI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HindIII KasI KpnI MfeI MluI NarI NdeI NheI NotI NruI NsiI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI