################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## Acc65I AclI AcsI AgeI Alw44I Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspI AvrII BbeI BcgI BclI BfaI BglII BlnI BlpI Bpu1102I BsaBI BsiWI Bsp1407I BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CelII CpoI DraI DraIII Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 2135 GACGTC GACGT^C AccI 848 GTAGAC GT^[AC][GT]AC AccIII 2036 TCCGGA T^CCGGA AcyI 2135 GACGTC G[GA]^CG[CT]C AflII 604 CTTAAG C^TTAAG AflIII 1599 ACACGT A^C[GA][CT]GT AhdI 991 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 214 CCTGAGG CC^T[ACGT]AGG AscI 2127 GGCGCGCC GG^CGCGCC AspEI 991 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1247 TGCGCA TGC^GCA BbrPI 1492 CACGTG CAC^GTG BciVI 706 GGATAC GTATCC(6/5) BfrI 604 CTTAAG C^TTAAG BsaHI 2135 GACGTC G[GA]^CG[CT]C BsaI 837 GGTCTC GGTCTC(1/5) BsaWI 2036 TCCGGA [AT]^CCGG[AT] BseAI 2036 TCCGGA T^CCGGA BsiEI 1577 CGGCCG CG[GA][CT]^CG BsmBI 776 GAGACG CGTCTC(1/5) BsmI 267 GCATTC GAATGC(1/-1) BspDI 529 ATCGAT AT^CGAT BspEI 2036 TCCGGA T^CCGGA BsrBI 1231 GAGCGG CCGCTC(-3/-3) BssHII 2128 GCGCGC G^CGCGC BssSI 1387 CACGAG CACGAG(-5/-1) BstAPI 267 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 214 CCTGAGG CC^T[ACGT]AGG ClaI 529 ATCGAT AT^CGAT DrdI 2419 GACGAGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1577 CGGCCG C^GGCCG Eam1105I 991 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1577 CGGCCG C^GGCCG Eco52I 1577 CGGCCG C^GGCCG Eco81I 214 CCTGAGG CC^T[ACGT]AGG EcoNI 315 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1247 TGCGCA TGC^GCA Hin1I 2135 GACGTC G[GA]^CG[CT]C HincII 901 GTCAAC GT[CT]^[GA]AC HindII 901 GTCAAC GT[CT]^[GA]AC MroI 2036 TCCGGA T^CCGGA NcoI 1710 CCATGG C^CATGG PmaCI 1492 CACGTG CAC^GTG PmlI 1492 CACGTG CAC^GTG PpuMI 1720 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 1997 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1836 GAAGAGC GCTCTTC(1/4) SfiI 1289 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### ApaI 1288 GGGCCC GGGCC^C 1670 GGGCCC BalI 1467 TGGCCA TGG^CCA 1496 TGGCCA BbsI 794 GAAGAC GAAGAC(2/6) 1956 GTCTTC BbvCI 2029 CCTCAGC CCTCAGC(-5/-2) 1434 GCTGAGG BglI 1290 GCCCCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1816 GCCAAGAAGGC BpuAI 794 GAAGAC GAAGAC(2/6) 1956 GTCTTC BspHI 515 TCATGA T^CATGA 1983 TCATGA BstXI 669 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1452 CCAGTCGCCTGG DsaI 1710 CCATGG C^C[GA][CT]GG 1950 CCACGG FseI 1578 GGCCGGCC GGCCGG^CC 2022 GGCCGGCC HgaI 778 GACGC GACGC(5/10) 1573 GACGC MluNI 1467 TGGCCA TGG^CCA 1496 TGGCCA MscI 1467 TGGCCA TGG^CCA 1496 TGGCCA RcaI 515 TCATGA T^CATGA 1983 TCATGA SacI 672 GAGCTC GAGCT^C 1171 GAGCTC SmlI 604 CTTAAG C^T[CT][GA]AG 2008 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BamHI BanI BanII BbvI BcnI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII