(Circular) (Six-base) MAPSORT of: pFN21AE2111.seq Check: 2707 from: 1 to: 7183 pFN21AE2111 7183 bp With 182 enzymes: SgfI * March 28, 2012 12:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3640 3640 Size: 7183 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 4993 278 Size: 53 83 186 936 2608 849 2468 Fragments arranged by size: 2608 2468 936 849 186 83 53 AccI GT'mk_AC Cuts at: 1937 2854 4526 1937 Size: 917 1672 4594 Fragments arranged by size: 4594 1672 917 Acc65I G'GTAC_C Cuts at: 4504 4504 Size: 7183 AclI AA'CG_TT Cuts at: 1563 5310 5683 1563 Size: 3747 373 3063 Fragments arranged by size: 3747 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2359 2438 3263 5246 6630 6898 1632 Size: 710 17 79 825 1983 1384 268 1917 Fragments arranged by size: 1983 1917 1384 825 710 268 79 17 AflII C'TTAA_G Cuts at: 820 1017 2609 820 Size: 197 1592 5394 Fragments arranged by size: 5394 1592 197 AflIII A'CryG_T Cuts at: 1203 3604 6082 7059 1203 Size: 2401 2478 977 1327 Fragments arranged by size: 2478 2401 1327 977 AgeI A'CCGG_T Cuts at: 6014 6014 Size: 7183 AhdI GACnn_n'nnGTC Cuts at: 3001 5912 3001 Size: 2911 4272 AleI CACnn'nnGTG Cuts at: 1166 3857 4292 1166 Size: 2691 435 4057 Fragments arranged by size: 4057 2691 435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3086 3875 1184 Size: 1902 789 4492 Fragments arranged by size: 4492 1902 789 AlwNI CAG_nnn'CTG Cuts at: 2081 2136 2815 2980 4926 6498 2081 Size: 55 679 165 1946 1572 2766 Fragments arranged by size: 2766 1946 1572 679 165 55 ApaI G_GGCC'C Cuts at: 3297 3679 3297 Size: 382 6801 ApaLI G'TGCA_C Cuts at: 5240 6396 5240 Size: 1156 6027 ApoI r'AATT_y Cuts at: 1493 4492 4718 4870 6979 1493 Size: 2999 226 152 2109 1697 Fragments arranged by size: 2999 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 4133 4133 Size: 7183 AseI AT'TA_AT Cuts at: 160 1032 5737 160 Size: 872 4705 1606 Fragments arranged by size: 4705 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7183 AvaI C'yCGr_G Cuts at: 1870 1943 2113 2836 3535 3729 4508 1870 Size: 73 170 723 699 194 779 4545 Fragments arranged by size: 4545 779 723 699 194 170 73 BamHI G'GATC_C Cuts at: 2888 3030 3045 4513 4880 2888 Size: 142 15 1468 367 5191 Fragments arranged by size: 5191 1468 367 142 15 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3100 3133 3336 3527 3859 Size: 325 217 627 1313 33 203 191 332 Cuts at: 3859 3866 3939 4504 5959 618 Size: 7 73 565 1455 1842 Fragments arranged by size: 1842 1455 1313 627 565 332 325 217 203 191 73 33 7 BanII G_rGCy'C Cuts at: 1391 2391 2657 2681 3180 3297 3381 3679 4502 Size: 1000 266 24 499 117 84 298 823 Cuts at: 4502 1391 Size: 4072 Fragments arranged by size: 4072 1000 823 499 298 266 117 84 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2806 3954 928 Size: 971 907 1148 4157 Fragments arranged by size: 4157 1148 971 907 BbvCI CC'TCA_GC Cuts at: 3440 4035 3440 Size: 595 6588 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5374 5408 1903 Size: 34 3437 34 3678 Fragments arranged by size: 3678 3437 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2705 5075 6285 2705 Size: 2370 1210 3603 Fragments arranged by size: 3603 2370 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7183 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3301 3827 5794 Size: 107 122 71 631 605 1629 526 1967 Cuts at: 5794 136 Size: 1525 Fragments arranged by size: 1967 1629 1525 631 605 526 122 107 71 BglII A'GATC_T Cuts at: 7178 7178 Size: 7183 BlpI GC'TnA_GC Cuts at: 4600 4600 Size: 7183 Bme1580I G_kGCm'C Cuts at: 1790 3105 3297 3498 3543 3679 4228 5244 6400 Size: 1315 192 201 45 136 549 1016 1156 Cuts at: 6400 1790 Size: 2573 Fragments arranged by size: 2573 1315 1156 1016 549 201 192 136 45 BmgBI CAC'GTC Cuts at: 1318 3607 3619 1318 Size: 2289 12 4882 Fragments arranged by size: 4882 2289 12 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2069 3530 4914 Size: 416 53 83 399 24 652 1461 1384 Cuts at: 4914 5872 442 Size: 958 1753 Fragments arranged by size: 1753 1461 1384 958 652 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7183 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2405 2833 2843 3212 3251 3982 4145 4166 Size: 1166 428 10 369 39 731 163 21 Cuts at: 4166 5843 1239 Size: 1677 2579 Fragments arranged by size: 2579 1677 1166 731 428 369 163 39 21 10 Bpu10I CC'TnA_GC Cuts at: 3440 4035 3440 Size: 595 6588 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4034 5308 6173 6471 4034 Size: 1274 865 298 4746 Fragments arranged by size: 4746 1274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2848 5846 882 Size: 617 1349 2998 2219 Fragments arranged by size: 2998 2219 1349 617 BsaAI yAC'GTr Cuts at: 493 1204 3126 3427 3499 3553 4974 493 Size: 711 1922 301 72 54 1421 2702 Fragments arranged by size: 2702 1922 1421 711 301 72 54 BsaBI GATnn'nnATC Cuts at: 7177 7177 Size: 7183 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4141 4990 5372 6007 Size: 53 83 186 936 2608 849 382 635 Cuts at: 6007 275 Size: 1451 Fragments arranged by size: 2608 1451 936 849 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4041 5616 6014 6288 6435 6846 4041 Size: 1575 398 274 147 411 4378 Fragments arranged by size: 4378 1575 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2085 2115 2167 2197 1178 Size: 30 165 30 682 30 52 30 6164 Fragments arranged by size: 6164 682 165 52 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2420 2836 3188 3236 3248 3275 3410 Size: 916 318 416 352 48 12 27 135 Cuts at: 3410 4142 4163 4424 4463 1186 Size: 732 21 261 39 3906 Fragments arranged by size: 3906 916 732 416 352 318 261 135 48 39 27 21 12 BseYI C'CCAG_C Cuts at: 2944 4099 4119 4227 6386 6882 2944 Size: 1155 20 108 2159 496 3245 Fragments arranged by size: 3245 2159 1155 496 108 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2350 3104 3221 3575 4073 1163 Size: 573 614 754 117 354 498 4273 Fragments arranged by size: 4273 754 614 573 498 354 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3585 4633 5394 6422 1540 Size: 275 186 1584 1048 761 1028 2301 Fragments arranged by size: 2301 1584 1048 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2681 3180 3426 4020 4177 5244 5329 6400 2681 Size: 499 246 594 157 1067 85 1071 3464 Fragments arranged by size: 3464 1071 1067 594 499 246 157 85 BsmI GAATG_Cn' Cuts at: 2272 4694 4787 2272 Size: 2422 93 4668 Fragments arranged by size: 4668 2422 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2775 2775 Size: 7183 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2391 2657 2681 3105 3180 3297 3381 Size: 399 601 266 24 424 75 117 84 Cuts at: 3381 3426 3498 3543 3679 4020 4177 4228 4502 Size: 45 72 45 136 341 157 51 274 Cuts at: 4502 5244 5329 6400 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 601 424 399 341 274 266 157 136 117 85 84 75 72 51 45 45 24 BspEI T'CCGG_A Cuts at: 4041 6846 4041 Size: 2805 4378 BspHI T'CATG_A Cuts at: 2520 3988 5072 6802 2520 Size: 1468 1084 1730 2901 Fragments arranged by size: 2901 1730 1468 1084 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2724 3076 3640 4538 844 Size: 1880 352 564 898 3489 Fragments arranged by size: 3489 1880 898 564 352 BsrBI CCG'CTC Cuts at: 1394 1823 3238 5070 1394 Size: 429 1415 1832 3507 Fragments arranged by size: 3507 1832 1415 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5678 5852 65 Size: 1183 4430 174 1396 Fragments arranged by size: 4430 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2927 3584 3950 4028 5827 6014 1700 Size: 110 1117 657 366 78 1799 187 2869 Fragments arranged by size: 2869 1799 1117 657 366 187 110 78 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7183 BssHII G'CGCG_C Cuts at: 1925 4133 1925 Size: 2208 4975 BssSI C'ACGA_G Cuts at: 3392 5243 6255 3392 Size: 1851 1012 4320 Fragments arranged by size: 4320 1851 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2278 794 Size: 1484 5699 BstBI TT'CG_AA Cuts at: 5987 5987 Size: 7183 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7183 BstXI CCAn_nnnn'nTGG Cuts at: 2681 3464 2681 Size: 783 6400 BstYI r'GATC_y Cuts at: 1972 2197 2888 3030 3045 4513 4880 5265 5282 Size: 225 691 142 15 1468 367 385 17 Cuts at: 5282 6734 6820 6832 7178 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1468 1452 691 385 367 346 225 142 86 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2220 2220 Size: 7183 BtgI C'CryG_G Cuts at: 513 1065 3715 3955 513 Size: 552 2650 240 3741 Fragments arranged by size: 3741 2650 552 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3003 3461 487 Size: 683 56 1777 458 4209 Fragments arranged by size: 4209 1777 683 458 56 BtsI GCAGTG_nn' Cuts at: 2233 2267 3911 4699 5493 5513 2233 Size: 34 1644 788 794 20 3903 Fragments arranged by size: 3903 1644 794 788 34 20 ClaI AT'CG_AT Cuts at: 1554 2535 1554 Size: 981 6202 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2232 2267 529 Size: 35 1668 35 5445 Fragments arranged by size: 5445 1668 35 35 DraI TTT'AAA Cuts at: 4487 4487 Size: 7183 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4430 6190 809 Size: 507 3114 1760 1802 Fragments arranged by size: 3114 1802 1760 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2178 3451 3472 Size: 54 1425 50 275 18 348 1273 21 Cuts at: 3472 3501 3582 3952 4630 5519 7148 8 Size: 29 81 370 678 889 1629 43 Fragments arranged by size: 1629 1425 1273 889 678 370 348 275 81 54 50 43 29 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3582 4630 1537 Size: 275 1770 1048 4090 Fragments arranged by size: 4090 1770 1048 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2405 3836 4073 5113 1353 Size: 1052 1431 237 1040 3423 Fragments arranged by size: 3423 1431 1052 1040 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5770 6144 6290 387 Size: 1064 138 4181 374 146 1280 Fragments arranged by size: 4181 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2679 3178 2679 Size: 499 6684 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2359 2405 2438 2833 2843 3212 Size: 393 710 17 46 33 395 10 369 Cuts at: 3212 3251 3263 3982 4145 4166 5246 5843 6630 Size: 39 12 719 163 21 1080 597 787 Cuts at: 6630 6898 1239 Size: 268 1524 Fragments arranged by size: 1524 1080 787 719 710 597 395 393 369 268 163 46 39 33 21 17 12 10 EcoNI CCTnn'n_nnAGG Cuts at: 2324 2324 Size: 7183 EcoO109I rG'GnC_Cy Cuts at: 3293 3294 3675 3697 3726 3293 Size: 1 381 22 29 6750 Fragments arranged by size: 6750 381 29 22 1 EcoRI G'AATT_C Cuts at: 4492 4870 4492 Size: 378 6805 FseI GG_CCGG'CC Cuts at: 3588 4032 3588 Size: 444 6739 FspI TGC'GCA Cuts at: 3254 5689 3254 Size: 2435 4748 FspAI rTGC'GCAy Cuts at: 3254 3254 Size: 7183 HaeII r_GCGC'y Cuts at: 1678 2194 3756 3849 6330 1678 Size: 516 1562 93 2481 2531 Fragments arranged by size: 2531 2481 1562 516 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2167 2199 2663 2695 2987 Size: 32 135 32 595 32 464 32 292 Cuts at: 2987 3019 3272 3304 5824 5856 5898 5930 1373 Size: 32 253 32 2520 32 42 32 2626 Fragments arranged by size: 2626 2520 595 464 292 253 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2908 4527 4773 669 Size: 988 281 970 1619 246 3079 Fragments arranged by size: 3079 1619 988 970 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7183 HpaI GTT'AAC Cuts at: 4773 4773 Size: 7183 KpnI G_GTAC'C Cuts at: 4508 4508 Size: 7183 MfeI C'AATT_G Cuts at: 4782 4782 Size: 7183 MluI A'CGCG_T Cuts at: 7059 7059 Size: 7183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6297 6481 1305 Size: 4992 184 2007 Fragments arranged by size: 4992 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3474 3503 10 Size: 54 1425 343 1642 29 3690 Fragments arranged by size: 3690 1642 1425 343 54 29 MslI CAynn'nnrTG Cuts at: 518 1166 3714 3857 4247 4292 5141 5500 5659 Size: 648 2548 143 390 45 849 359 159 Cuts at: 5659 518 Size: 2042 Fragments arranged by size: 2548 2042 849 648 390 359 159 143 45 MspA1I CmG'CkG Cuts at: 1606 2530 2831 3354 4123 4599 5276 6424 6669 Size: 924 301 523 769 476 677 1148 245 Cuts at: 6669 1606 Size: 2120 Fragments arranged by size: 2120 1148 924 769 677 523 476 301 245 NaeI GCC'GGC Cuts at: 2929 3586 4030 2929 Size: 657 444 6082 Fragments arranged by size: 6082 657 444 NcoI C'CATG_G Cuts at: 513 1065 3715 513 Size: 552 2650 3981 Fragments arranged by size: 3981 2650 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7183 NgoMIV G'CCGG_C Cuts at: 2927 3584 4028 2927 Size: 657 444 6082 Fragments arranged by size: 6082 657 444 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7183 NotI GC'GGCC_GC Cuts at: 4630 4630 Size: 7183 NspI r_CATG'y Cuts at: 1132 1229 4541 6086 1132 Size: 97 3312 1545 2229 Fragments arranged by size: 3312 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3299 4023 4315 1348 Size: 1951 724 292 4216 Fragments arranged by size: 4216 1951 724 292 PciI A'CATG_T Cuts at: 6082 6082 Size: 7183 PflMI CCAn_nnn'nTGG Cuts at: 1108 2081 2761 3190 1108 Size: 973 680 429 5101 Fragments arranged by size: 5101 973 680 429 PfoI T'CCnGG_A Cuts at: 2141 2820 3381 3414 2141 Size: 679 561 33 5910 Fragments arranged by size: 5910 679 561 33 PmeI GTTT'AAAC Cuts at: 4487 4487 Size: 7183 PmlI CAC'GTG Cuts at: 3499 3499 Size: 7183 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5252 5284 6765 6797 5252 Size: 32 1481 32 5638 Fragments arranged by size: 5638 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3726 3726 Size: 7183 PshAI GACnn'nnGTC Cuts at: 1613 4006 1613 Size: 2393 4790 PsiI TTA'TAA Cuts at: 4753 4753 Size: 7183 PspOMI G'GGCC_C Cuts at: 3293 3675 3293 Size: 382 6801 PstI C_TGCA'G Cuts at: 830 4535 830 Size: 3705 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7183 PvuII CAG'CTG Cuts at: 2530 2831 4123 2530 Size: 301 1292 5590 Fragments arranged by size: 5590 1292 301 SacI G_AGCT'C Cuts at: 2681 3180 2681 Size: 499 6684 SalI G'TCGA_C Cuts at: 1936 4525 1936 Size: 2589 4594 SapI GCTCTTCn'nnn_ Cuts at: 3836 3836 Size: 7183 SbfI CC_TGCA'GG Cuts at: 4535 4535 Size: 7183 ScaI AGT'ACT Cuts at: 5431 7143 5431 Size: 1712 5471 SfcI C'TryA_G Cuts at: 826 1045 2128 2665 4354 4531 5666 6347 6538 Size: 219 1083 537 1689 177 1135 681 191 Cuts at: 6538 826 Size: 1471 Fragments arranged by size: 1689 1471 1135 1083 681 537 219 191 177 SfiI GGCCn_nnn'nGGCC Cuts at: 3301 3301 Size: 7183 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7183 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7183 SmaI CCC'GGG Cuts at: 1872 4510 1872 Size: 2638 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2609 4013 5287 6188 6450 820 Size: 197 926 666 1404 1274 901 262 1553 Fragments arranged by size: 1553 1404 1274 926 901 666 262 197 SnaBI TAC'GTA Cuts at: 493 4974 493 Size: 4481 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7183 SphI G_CATG'C Cuts at: 1132 4541 1132 Size: 3409 3774 SspI AAT'ATT Cuts at: 5 52 5107 5 Size: 47 5055 2081 Fragments arranged by size: 5055 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7183 StyI C'CwwG_G Cuts at: 513 1065 2031 3642 3715 4622 513 Size: 552 966 1611 73 907 3074 Fragments arranged by size: 3074 1611 966 907 552 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2579 4113 5210 5227 5380 5565 6010 Size: 1255 999 1534 1097 17 153 185 445 Cuts at: 6010 325 Size: 1498 Fragments arranged by size: 1534 1498 1255 1097 999 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2445 5429 7141 Size: 274 80 33 51 1443 468 2984 1712 Cuts at: 7141 96 Size: 138 Fragments arranged by size: 2984 1712 1443 468 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2085 2117 2732 2764 7143 7175 2085 Size: 32 615 32 4379 32 2093 Fragments arranged by size: 4379 2093 615 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7183 XbaI T'CTAG_A Cuts at: 4519 4519 Size: 7183 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2253 3511 3649 2253 Size: 1258 138 5787 Fragments arranged by size: 5787 1258 138 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7183 XmaI C'CCGG_G Cuts at: 1870 4508 1870 Size: 2638 4545 XmnI GAAnn'nnTTC Cuts at: 5312 5312 Size: 7183 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 4991 276 Size: 53 83 186 936 2608 849 2468 Fragments arranged by size: 2608 2468 936 849 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI