(Linear) (Six-base) MAPSORT of: KIEE2111.seq Check: 7163 from: 1 to: 2478 KIEE2111.orf With 182 enzymes: SgfI * March 22, 2012 15:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1635 2478 Size: 1635 843 AatII G_ACGT'C Cuts at: 0 2139 2478 Size: 2139 339 AccI GT'mk_AC Cuts at: 0 849 2478 Size: 849 1629 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 354 433 1258 2478 Size: 337 17 79 825 1220 Fragments arranged by size: 1220 825 337 79 17 AflII C'TTAA_G Cuts at: 0 604 2478 Size: 604 1874 AflIII A'CryG_T Cuts at: 0 1599 2478 Size: 1599 879 AhdI GACnn_n'nnGTC Cuts at: 0 996 2478 Size: 996 1482 AleI CACnn'nnGTG Cuts at: 0 1852 2287 2478 Size: 1852 435 191 Fragments arranged by size: 1852 435 191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1081 1870 2478 Size: 1081 789 608 Fragments arranged by size: 1081 789 608 AlwNI CAG_nnn'CTG Cuts at: 0 76 131 810 975 2478 Size: 76 55 679 165 1503 Fragments arranged by size: 1503 679 165 76 55 ApaI G_GGCC'C Cuts at: 0 1292 1674 2478 Size: 1292 382 804 Fragments arranged by size: 1292 804 382 AscI GG'CGCG_CC Cuts at: 0 2128 2478 Size: 2128 350 AvaI C'yCGr_G Cuts at: 0 108 831 1530 1724 2478 Size: 108 723 699 194 754 Fragments arranged by size: 754 723 699 194 108 BamHI G'GATC_C Cuts at: 0 883 1025 1040 2478 Size: 883 142 15 1438 Fragments arranged by size: 1438 883 142 15 BanI G'GyrC_C Cuts at: 0 1095 1128 1331 1522 1854 1861 1934 2478 Size: 1095 33 203 191 332 7 73 544 Fragments arranged by size: 1095 544 332 203 191 73 33 7 BanII G_rGCy'C Cuts at: 0 386 652 676 1175 1292 1376 1674 2478 Size: 386 266 24 499 117 84 298 804 Fragments arranged by size: 804 499 386 298 266 117 84 24 BbsI GAAGACnn'nnnn_ Cuts at: 0 801 1949 2478 Size: 801 1148 529 Fragments arranged by size: 1148 801 529 BbvCI CC'TCA_GC Cuts at: 0 1435 2030 2478 Size: 1435 595 448 Fragments arranged by size: 1435 595 448 BciVI GTATCCnnnnn_n' Cuts at: 0 700 2478 Size: 700 1778 BglI GCCn_nnn'nGGC Cuts at: 0 1296 1822 2478 Size: 1296 526 656 Fragments arranged by size: 1296 656 526 Bme1580I G_kGCm'C Cuts at: 0 1100 1292 1493 1538 1674 2223 2478 Size: 1100 192 201 45 136 549 255 Fragments arranged by size: 1100 549 255 201 192 136 45 BmgBI CAC'GTC Cuts at: 0 1602 1614 2478 Size: 1602 12 864 Fragments arranged by size: 1602 864 12 BmrI ACTGGGnnnn_n' Cuts at: 0 64 1525 2478 Size: 64 1461 953 Fragments arranged by size: 1461 953 64 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 400 828 838 1207 1246 1977 2140 2161 Size: 400 428 10 369 39 731 163 21 Cuts at: 2161 2478 Size: 317 Fragments arranged by size: 731 428 400 369 317 163 39 21 10 Bpu10I CC'TnA_GC Cuts at: 0 1435 2030 2478 Size: 1435 595 448 Fragments arranged by size: 1435 595 448 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2029 2478 Size: 2029 449 BsaI GGTCTCn'nnnn_ Cuts at: 0 843 2478 Size: 843 1635 BsaAI yAC'GTr Cuts at: 0 1121 1422 1494 1548 2478 Size: 1121 301 72 54 930 Fragments arranged by size: 1121 930 301 72 54 BsaHI Gr'CG_yC Cuts at: 0 2136 2478 Size: 2136 342 BsaWI w'CCGG_w Cuts at: 0 2036 2478 Size: 2036 442 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 80 110 162 192 2478 Size: 80 30 52 30 2286 Fragments arranged by size: 2286 80 52 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 97 415 831 1183 1231 1243 1270 1405 Size: 97 318 416 352 48 12 27 135 Cuts at: 1405 2137 2158 2419 2458 2478 Size: 732 21 261 39 20 Fragments arranged by size: 732 416 352 318 261 135 97 48 39 27 21 20 12 BseYI C'CCAG_C Cuts at: 0 939 2094 2114 2222 2478 Size: 939 1155 20 108 256 Fragments arranged by size: 1155 939 256 108 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 345 1099 1216 1570 2068 2478 Size: 345 754 117 354 498 410 Fragments arranged by size: 754 498 410 354 345 117 BsiEI CG_ry'CG Cuts at: 0 1580 2478 Size: 1580 898 BsiHKAI G_wGCw'C Cuts at: 0 676 1175 1421 2015 2172 2478 Size: 676 499 246 594 157 306 Fragments arranged by size: 676 594 499 306 246 157 BsmI GAATG_Cn' Cuts at: 0 267 2478 Size: 267 2211 BsmBI CGTCTCn'nnnn_ Cuts at: 0 770 2478 Size: 770 1708 Bsp1286I G_dGCh'C Cuts at: 0 386 652 676 1100 1175 1292 1376 1421 Size: 386 266 24 424 75 117 84 45 Cuts at: 1421 1493 1538 1674 2015 2172 2223 2478 Size: 72 45 136 341 157 51 255 Fragments arranged by size: 424 386 341 266 255 157 136 117 84 75 72 51 45 45 24 BspEI T'CCGG_A Cuts at: 0 2036 2478 Size: 2036 442 BspHI T'CATG_A Cuts at: 0 515 1983 2478 Size: 515 1468 495 Fragments arranged by size: 1468 515 495 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 719 1071 1635 2478 Size: 719 352 564 843 Fragments arranged by size: 843 719 564 352 BsrBI CCG'CTC Cuts at: 0 1233 2478 Size: 1233 1245 BsrFI r'CCGG_y Cuts at: 0 922 1579 1945 2023 2478 Size: 922 657 366 78 455 Fragments arranged by size: 922 657 455 366 78 BssHII G'CGCG_C Cuts at: 0 2128 2478 Size: 2128 350 BssSI C'ACGA_G Cuts at: 0 1387 2478 Size: 1387 1091 BstAPI GCAn_nnn'nTGC Cuts at: 0 273 2478 Size: 273 2205 BstXI CCAn_nnnn'nTGG Cuts at: 0 676 1459 2478 Size: 676 783 1019 Fragments arranged by size: 1019 783 676 BstYI r'GATC_y Cuts at: 0 192 883 1025 1040 2478 Size: 192 691 142 15 1438 Fragments arranged by size: 1438 691 192 142 15 Bsu36I CC'TnA_GG Cuts at: 0 215 2478 Size: 215 2263 BtgI C'CryG_G Cuts at: 0 1710 1950 2478 Size: 1710 240 528 Fragments arranged by size: 1710 528 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 998 1456 2478 Size: 998 458 1022 Fragments arranged by size: 1022 998 458 BtsI GCAGTG_nn' Cuts at: 0 228 262 1906 2478 Size: 228 34 1644 572 Fragments arranged by size: 1644 572 228 34 ClaI AT'CG_AT Cuts at: 0 530 2478 Size: 530 1948 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 227 262 2478 Size: 227 35 2216 Fragments arranged by size: 2216 227 35 DrdI GACnn_nn'nnGTC Cuts at: 0 2425 2478 Size: 2425 53 EaeI y'GGCC_r Cuts at: 0 173 1446 1467 1496 1577 1947 2478 Size: 173 1273 21 29 81 370 531 Fragments arranged by size: 1273 531 370 173 81 29 21 EagI C'GGCC_G Cuts at: 0 1577 2478 Size: 1577 901 EarI CTCTTCn'nnn_ Cuts at: 0 400 1831 2068 2478 Size: 400 1431 237 410 Fragments arranged by size: 1431 410 400 237 EcoICRI GAG'CTC Cuts at: 0 674 1173 2478 Size: 674 499 1305 Fragments arranged by size: 1305 674 499 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 354 400 433 828 838 1207 1246 Size: 337 17 46 33 395 10 369 39 Cuts at: 1246 1258 1977 2140 2161 2478 Size: 12 719 163 21 317 Fragments arranged by size: 719 395 369 337 317 163 46 39 33 21 17 12 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 319 2478 Size: 319 2159 EcoO109I rG'GnC_Cy Cuts at: 0 1288 1289 1670 1692 1721 2478 Size: 1288 1 381 22 29 757 Fragments arranged by size: 1288 757 381 29 22 1 FseI GG_CCGG'CC Cuts at: 0 1583 2027 2478 Size: 1583 444 451 Fragments arranged by size: 1583 451 444 FspI TGC'GCA Cuts at: 0 1249 2478 Size: 1249 1229 FspAI rTGC'GCAy Cuts at: 0 1249 2478 Size: 1249 1229 HaeII r_GCGC'y Cuts at: 0 189 1751 1844 2478 Size: 189 1562 93 634 Fragments arranged by size: 1562 634 189 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 162 194 658 690 982 1014 1267 1299 Size: 162 32 464 32 292 32 253 32 Cuts at: 1299 2478 Size: 1179 Fragments arranged by size: 1179 464 292 253 162 32 32 32 32 HincII GTy'rAC Cuts at: 0 903 2478 Size: 903 1575 MscI TGG'CCA Cuts at: 0 1469 1498 2478 Size: 1469 29 980 Fragments arranged by size: 1469 980 29 MslI CAynn'nnrTG Cuts at: 0 1709 1852 2242 2287 2478 Size: 1709 143 390 45 191 Fragments arranged by size: 1709 390 191 143 45 MspA1I CmG'CkG Cuts at: 0 525 826 1349 2118 2478 Size: 525 301 523 769 360 Fragments arranged by size: 769 525 523 360 301 NaeI GCC'GGC Cuts at: 0 924 1581 2025 2478 Size: 924 657 444 453 Fragments arranged by size: 924 657 453 444 NcoI C'CATG_G Cuts at: 0 1710 2478 Size: 1710 768 NgoMIV G'CCGG_C Cuts at: 0 922 1579 2023 2478 Size: 922 657 444 455 Fragments arranged by size: 922 657 455 444 PasI CC'CwG_GG Cuts at: 0 1294 2018 2310 2478 Size: 1294 724 292 168 Fragments arranged by size: 1294 724 292 168 PflMI CCAn_nnn'nTGG Cuts at: 0 76 756 1185 2478 Size: 76 680 429 1293 Fragments arranged by size: 1293 680 429 76 PfoI T'CCnGG_A Cuts at: 0 136 815 1376 1409 2478 Size: 136 679 561 33 1069 Fragments arranged by size: 1069 679 561 136 33 PmlI CAC'GTG Cuts at: 0 1494 2478 Size: 1494 984 PpuMI rG'GwC_Cy Cuts at: 0 1721 2478 Size: 1721 757 PshAI GACnn'nnGTC Cuts at: 0 2001 2478 Size: 2001 477 PspOMI G'GGCC_C Cuts at: 0 1288 1670 2478 Size: 1288 382 808 Fragments arranged by size: 1288 808 382 PvuII CAG'CTG Cuts at: 0 525 826 2118 2478 Size: 525 301 1292 360 Fragments arranged by size: 1292 525 360 301 SacI G_AGCT'C Cuts at: 0 676 1175 2478 Size: 676 499 1303 Fragments arranged by size: 1303 676 499 SapI GCTCTTCn'nnn_ Cuts at: 0 1831 2478 Size: 1831 647 SfcI C'TryA_G Cuts at: 0 123 660 2349 2478 Size: 123 537 1689 129 Fragments arranged by size: 1689 537 129 123 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1296 2478 Size: 1296 1182 SmlI C'TyrA_G Cuts at: 0 604 2008 2478 Size: 604 1404 470 Fragments arranged by size: 1404 604 470 StyI C'CwwG_G Cuts at: 0 26 1637 1710 2478 Size: 26 1611 73 768 Fragments arranged by size: 1611 768 73 26 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 574 2108 2478 Size: 574 1534 370 Fragments arranged by size: 1534 574 370 TatI w'GTAC_w Cuts at: 0 440 2478 Size: 440 2038 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 80 112 727 759 2478 Size: 80 32 615 32 1719 Fragments arranged by size: 1719 615 80 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 248 1506 1644 2478 Size: 248 1258 138 834 Fragments arranged by size: 1258 834 248 138 ZraI GAC'GTC Cuts at: 0 2137 2478 Size: 2137 341 Enzymes that do cut: AarI AatII AccI AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaI AscI AvaI BamHI BanI BanII BbsI BbvCI BciVI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I HincII MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PvuII SacI SapI SfcI SfiI SmlI StyI TaqII TatI TstI XcmI ZraI Enzymes that do not cut: Acc65I AclI AfeI AgeI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglII BlpI BmtI BplI BsaBI BsiWI BsrDI BsrGI BstBI BstEII BstZ17I DraI DraIII EciI EcoRI EcoRV FalI HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI