################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcsI 953 AAATTT [GA]^AATT[CT] Alw44I 654 GTGCAC G^TGCAC AlwI 3 GGATC GGATC(4/5) ApaLI 654 GTGCAC G^TGCAC ApoI 953 AAATTT [GA]^AATT[CT] BbsI 1002 GAAGAC GAAGAC(2/6) BclI 878 TGATCA T^GATCA BfaI 707 CTAG C^TAG BpuAI 1002 GAAGAC GAAGAC(2/6) BsaJI 620 CCACGG C^C[ACGT][ACGT]GG BsgI 380 CTGCAC GTGCAG(16/14) BsiYI 385 CCCTTGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 385 CCCTTGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 312 GTCCC GGGAC(10/14) Bsp1407I 714 TGTACA T^GTACA BspHI 596 TCATGA T^CATGA BsrDI 540 CATTGC GCAATG(2/0) BsrGI 714 TGTACA T^GTACA BstAPI 544 GCACTTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 545 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 620 CCACGG C^C[GA][CT]GG FbaI 878 TGATCA T^GATCA HincII 306 GTCAAC GT[CT]^[GA]AC HindII 306 GTCAAC GT[CT]^[GA]AC HphI 818 TCACC GGTGA(8/7) MaeI 707 CTAG C^TAG MaeII 310 ACGT A^CGT PstI 290 CTGCAG CTGCA^G RcaI 596 TCATGA T^CATGA SfaNI 93 GATGC GCATC(5/9) SspBI 714 TGTACA T^GTACA SspI 493 AATATT AAT^ATT StuI 807 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AfaI 478 GTAC GT^AC 715 GTAC AocI 49 CCTCAGG CC^T[ACGT]AGG 335 CCTCAGG AspHI 654 GTGCAC G[AT]GC[AT]^C 814 GTGCTC BciVI 408 GTATCC GTATCC(6/5) 499 GTATCC BcnI 67 CCCGG CC^[GC]GG 391 CCGGG BsiHKAI 654 GTGCAC G[AT]GC[AT]^C 814 GTGCTC BsrI 106 ACTGG ACTGG(1/-1) 687 ACTGG BstNI 342 CCAGG CC^[AT]GG 859 CCTGG Bsu36I 49 CCTCAGG CC^T[ACGT]AGG 335 CCTCAGG DpnII 4 GATC ^GATC 879 GATC EarI 355 CTCTTC CTCTTC(1/4) 157 GAAGAG Eco81I 49 CCTCAGG CC^T[ACGT]AGG 335 CCTCAGG EcoRII 342 CCAGG ^CC[AT]GG 859 CCTGG HapII 68 CCGG C^CGG 391 CCGG HpaII 68 CCGG C^CGG 391 CCGG Ksp632I 355 CTCTTC CTCTTC(1/4) 157 GAAGAG MboI 4 GATC ^GATC 879 GATC MseI 865 TTAA T^TAA 928 TTAA MspA1I 287 CAGCTG C[AC]G^C[GT]G 484 CAGCTG MspI 68 CCGG C^CGG 391 CCGG MvaI 342 CCAGG CC^[AT]GG 859 CCTGG NciI 67 CCCGG CC^[GC]GG 391 CCGGG NdeII 4 GATC ^GATC 879 GATC PvuII 287 CAGCTG CAG^CTG 484 CAGCTG RsaI 478 GTAC GT^AC 715 GTAC SapI 157 GAAGAGC GCTCTTC(1/4) 354 GCTCTTC Sau3AI 4 GATC ^GATC 879 GATC SfcI 290 CTGCAG C^T[GA][CT]AG 870 CTACAG Tru9I 865 TTAA T^TAA 928 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI AluI BanI BbvI BmyI Bsp1286I BssKI Cac8I DdeI Fnu4HI HaeIII HinfI ItaI MboII MnlI MwoI NlaIII NlaIV PleI ScrFI TfiI TseI Tsp509I TspRI