################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BglI BlnI BseAI BsiWI Bsp1407I BspEI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I CpoI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MroI NaeI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 2511 GGTACC G^GTACC AccI 1656 GTCTAC GT^[AC][GT]AC AcyI 35 GGCGCC G[GA]^CG[CT]C ApaI 447 GGGCCC GGGCC^C Asp700I 2928 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2511 GGTACC G^GTACC BbeI 35 GGCGCC GGCGC^C BbsI 1284 GAAGAC GAAGAC(2/6) BglII 491 AGATCT A^GATCT BlpI 9 GCTGAGC GC^T[ACGT]AGC BpmI 2737 CTGGAG CTGGAG(16/14) Bpu1102I 9 GCTGAGC GC^T[ACGT]AGC BpuAI 1284 GAAGAC GAAGAC(2/6) BsaAI 1737 CACGTA [CT]AC^GT[GA] BsaBI 1167 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 35 GGCGCC G[GA]^CG[CT]C BsiEI 915 CGACCG CG[GA][CT]^CG BsmBI 1783 GAGACG CGTCTC(1/5) BsmI 1794 GAATGC GAATGC(1/-1) BspDI 322 ATCGAT AT^CGAT BspHI 3332 TCATGA T^CATGA BsrFI 3425 ACCGGC [GA]^CCGG[CT] BssSI 1478 CTCGTG CACGAG(-5/-1) BstAPI 43 GCACGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 710 GGTCACC G^GT[ACGT]ACC BstPI 710 GGTCACC G^GT[ACGT]ACC CelII 9 GCTGAGC GC^T[ACGT]AGC Cfr10I 3425 ACCGGC [GA]^CCGG[CT] ClaI 322 ATCGAT AT^CGAT DrdI 520 GACAGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 710 GGTCACC G^GT[ACGT]ACC EcoRV 319 GATATC GAT^ATC Hin1I 35 GGCGCC G[GA]^CG[CT]C KasI 35 GGCGCC G^GCGCC KpnI 2511 GGTACC GGTAC^C MamI 1167 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 692 CAATTG C^AATTG MluI 1786 ACGCGT A^CGCGT MunI 692 CAATTG C^AATTG NarI 35 GGCGCC GG^CGCC NcoI 2640 CCATGG C^CATGG NdeI 1106 CATATG CA^TATG NruI 3113 TCGCGA TCG^CGA PflMI 309 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 3332 TCATGA T^CATGA SapI 463 GAAGAGC GCTCTTC(1/4) ScaI 1023 AGTACT AGT^ACT SfoI 35 GGCGCC GGC^GCC SmaI 2807 CCCGGG CCC^GGG Van91I 309 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2807 CCCGGG C^CCGGG XmaI 2807 CCCGGG C^CCGGG XmnI 2928 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflII 1341 CTTAAG C^TTAAG 2071 CTTAAG Aor51HI 13 AGCGCT AGC^GCT 2054 AGCGCT AvaI 444 CTCGGG C^[CT]CG[GA]G 2807 CCCGGG BfrI 1341 CTTAAG C^TTAAG 2071 CTTAAG BsaI 3311 GAGACC GGTCTC(1/5) 160 GGTCTC BsaWI 2551 ACCGGA [AT]^CCGG[AT] 2943 ACCGGA BsoBI 444 CTCGGG C^[CT]CG[GA]G 2807 CCCGGG BspLU11I 2696 ACATGT A^CATGT 2897 ACATGT BstXI 1460 CCATTACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2501 CCAAGTTGTTGG BstYI 491 AGATCT [GA]^GATC[CT] 3139 AGATCC DraIII 1253 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG 1291 CACCCTGTG Eco47III 13 AGCGCT AGC^GCT 2054 AGCGCT EcoT14I 2234 CCAAGG C^C[AT][AT]GG 2640 CCATGG MflI 491 AGATCT [GA]^GATC[CT] 3139 AGATCC PpuMI 141 AGGACCC [GA]G^G[AT]CC[CT] 1051 AGGTCCT SmlI 1341 CTTAAG C^T[CT][GA]AG 2071 CTTAAG SphI 1899 GCATGC GCATG^C 3446 GCATGC StyI 2234 CCAAGG C^C[AT][AT]GG 2640 CCATGG XcmI 2498 CCACCAAGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2882 CCACCGAAACCGTGG XhoII 491 AGATCT [GA]^GATC[CT] 3139 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BanII BbvI BcgI BcnI BfaI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StuI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I