(Linear) (Six-base) MAPSORT of: KIBB9997.seq Check: 3433 from: 1 to: 3519 KIBB9997.orf With 182 enzymes: SgfI * March 22, 2012 15:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3404 3519 Size: 3404 115 AccI GT'mk_AC Cuts at: 0 1657 3519 Size: 1657 1862 Acc65I G'GTAC_C Cuts at: 0 2511 3519 Size: 2511 1008 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 473 482 2071 2767 2971 3519 Size: 473 9 1589 696 204 548 Fragments arranged by size: 1589 696 548 473 204 9 AfeI AGC'GCT Cuts at: 0 15 2056 3519 Size: 15 2041 1463 Fragments arranged by size: 2041 1463 15 AflII C'TTAA_G Cuts at: 0 1341 2071 3519 Size: 1341 730 1448 Fragments arranged by size: 1448 1341 730 AflIII A'CryG_T Cuts at: 0 1786 2696 2897 3519 Size: 1786 910 201 622 Fragments arranged by size: 1786 910 622 201 AleI CACnn'nnGTG Cuts at: 0 1480 3519 Size: 1480 2039 AlwNI CAG_nnn'CTG Cuts at: 0 981 1125 1456 2172 3435 3519 Size: 981 144 331 716 1263 84 Fragments arranged by size: 1263 981 716 331 144 84 ApaI G_GGCC'C Cuts at: 0 451 3519 Size: 451 3068 ApoI r'AATT_y Cuts at: 0 1677 1867 2145 2220 2281 3220 3519 Size: 1677 190 278 75 61 939 299 Fragments arranged by size: 1677 939 299 278 190 75 61 AvaI C'yCGr_G Cuts at: 0 444 2807 3519 Size: 444 2363 712 Fragments arranged by size: 2363 712 444 BanI G'GyrC_C Cuts at: 0 35 790 2511 2644 3361 3392 3519 Size: 35 755 1721 133 717 31 127 Fragments arranged by size: 1721 755 717 133 127 35 31 BanII G_rGCy'C Cuts at: 0 451 1265 1517 1970 2330 3519 Size: 451 814 252 453 360 1189 Fragments arranged by size: 1189 814 453 451 360 252 BbeI G_GCGC'C Cuts at: 0 39 3519 Size: 39 3480 BbsI GAAGACnn'nnnn_ Cuts at: 0 1291 3519 Size: 1291 2228 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 303 337 2665 2699 2703 2737 2806 2840 Size: 303 34 2328 34 4 34 69 34 Cuts at: 2840 3519 Size: 679 Fragments arranged by size: 2328 679 303 69 34 34 34 34 4 BglII A'GATC_T Cuts at: 0 491 3519 Size: 491 3028 BlpI GC'TnA_GC Cuts at: 0 10 3519 Size: 10 3509 Bme1580I G_kGCm'C Cuts at: 0 451 3519 Size: 451 3068 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 459 1154 2682 3519 Size: 459 695 1528 837 Fragments arranged by size: 1528 837 695 459 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2758 3519 Size: 2758 761 Bpu10I CC'TnA_GC Cuts at: 0 2843 3519 Size: 2843 676 BsaI GGTCTCn'nnnn_ Cuts at: 0 166 3305 3519 Size: 166 3139 214 Fragments arranged by size: 3139 214 166 BsaAI yAC'GTr Cuts at: 0 1739 3519 Size: 1739 1780 BsaBI GATnn'nnATC Cuts at: 0 1171 3519 Size: 1171 2348 BsaHI Gr'CG_yC Cuts at: 0 36 3519 Size: 36 3483 BsaWI w'CCGG_w Cuts at: 0 2551 2943 3519 Size: 2551 392 576 Fragments arranged by size: 2551 576 392 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 411 441 727 757 3519 Size: 411 30 286 30 2762 Fragments arranged by size: 2762 411 286 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 2671 3262 3519 Size: 19 2652 591 257 Fragments arranged by size: 2652 591 257 19 BseYI C'CCAG_C Cuts at: 0 183 3519 Size: 183 3336 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 536 975 2785 3519 Size: 536 439 1810 734 Fragments arranged by size: 1810 734 536 439 BsiEI CG_ry'CG Cuts at: 0 918 3519 Size: 918 2601 BsiHKAI G_wGCw'C Cuts at: 0 1216 1265 1517 1970 3108 3519 Size: 1216 49 252 453 1138 411 Fragments arranged by size: 1216 1138 453 411 252 49 BsmI GAATG_Cn' Cuts at: 0 1800 3519 Size: 1800 1719 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1777 3519 Size: 1777 1742 Bsp1286I G_dGCh'C Cuts at: 0 451 1216 1265 1517 1970 2330 3108 3519 Size: 451 765 49 252 453 360 778 411 Fragments arranged by size: 778 765 453 451 411 360 252 49 BspHI T'CATG_A Cuts at: 0 3332 3519 Size: 3332 187 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 880 2910 3404 3519 Size: 880 2030 494 115 Fragments arranged by size: 2030 880 494 115 BsrBI CCG'CTC Cuts at: 0 995 2864 3377 3519 Size: 995 1869 513 142 Fragments arranged by size: 1869 995 513 142 BsrDI GCAATG_nn' Cuts at: 0 1495 2975 3122 3519 Size: 1495 1480 147 397 Fragments arranged by size: 1495 1480 397 147 BsrFI r'CCGG_y Cuts at: 0 3425 3519 Size: 3425 94 BssSI C'ACGA_G Cuts at: 0 1478 3519 Size: 1478 2041 BstAPI GCAn_nnn'nTGC Cuts at: 0 49 3519 Size: 49 3470 BstEII G'GTnAC_C Cuts at: 0 710 3519 Size: 710 2809 BstXI CCAn_nnnn'nTGG Cuts at: 0 1467 2508 3519 Size: 1467 1041 1011 Fragments arranged by size: 1467 1041 1011 BstYI r'GATC_y Cuts at: 0 491 3139 3519 Size: 491 2648 380 Fragments arranged by size: 2648 491 380 BtgI C'CryG_G Cuts at: 0 2640 2891 3410 3519 Size: 2640 251 519 109 Fragments arranged by size: 2640 519 251 109 BtsI GCAGTG_nn' Cuts at: 0 240 2209 3519 Size: 240 1969 1310 Fragments arranged by size: 1969 1310 240 ClaI AT'CG_AT Cuts at: 0 323 3519 Size: 323 3196 DraI TTT'AAA Cuts at: 0 1236 2219 2613 3519 Size: 1236 983 394 906 Fragments arranged by size: 1236 983 906 394 DraIII CAC_nnn'GTG Cuts at: 0 1258 1296 3519 Size: 1258 38 2223 Fragments arranged by size: 2223 1258 38 DrdI GACnn_nn'nnGTC Cuts at: 0 526 3519 Size: 526 2993 EaeI y'GGCC_r Cuts at: 0 606 2405 2879 3257 3519 Size: 606 1799 474 378 262 Fragments arranged by size: 1799 606 474 378 262 EarI CTCTTCn'nnn_ Cuts at: 0 402 458 1775 2765 3519 Size: 402 56 1317 990 754 Fragments arranged by size: 1317 990 754 402 56 EcoICRI GAG'CTC Cuts at: 0 1263 1515 1968 3519 Size: 1263 252 453 1551 Fragments arranged by size: 1551 1263 453 252 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 473 482 2071 2758 2767 2971 3519 Size: 473 9 1589 687 9 204 548 Fragments arranged by size: 1589 687 548 473 204 9 9 EcoO109I rG'GnC_Cy Cuts at: 0 115 142 448 1052 1453 3519 Size: 115 27 306 604 401 2066 Fragments arranged by size: 2066 604 401 306 115 27 EcoRV GAT'ATC Cuts at: 0 321 3519 Size: 321 3198 HaeII r_GCGC'y Cuts at: 0 17 39 2058 2320 3519 Size: 17 22 2019 262 1199 Fragments arranged by size: 2019 1199 262 22 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 144 176 316 348 515 547 624 656 Size: 144 32 140 32 167 32 77 32 Cuts at: 656 2124 2156 2842 2874 3124 3156 3519 Size: 1468 32 686 32 250 32 363 Fragments arranged by size: 1468 686 363 250 167 144 140 77 32 32 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 35 3519 Size: 35 3484 KpnI G_GTAC'C Cuts at: 0 2515 3519 Size: 2515 1004 MfeI C'AATT_G Cuts at: 0 692 3519 Size: 692 2827 MluI A'CGCG_T Cuts at: 0 1786 3519 Size: 1786 1733 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 903 1741 3047 3519 Size: 903 838 1306 472 Fragments arranged by size: 1306 903 838 472 MscI TGG'CCA Cuts at: 0 608 2407 2881 3259 3519 Size: 608 1799 474 378 260 Fragments arranged by size: 1799 608 474 378 260 MslI CAynn'nnrTG Cuts at: 0 1145 1480 2428 2650 3331 3367 3519 Size: 1145 335 948 222 681 36 152 Fragments arranged by size: 1145 948 681 335 222 152 36 MspA1I CmG'CkG Cuts at: 0 480 2169 3096 3519 Size: 480 1689 927 423 Fragments arranged by size: 1689 927 480 423 NarI GG'CG_CC Cuts at: 0 36 3519 Size: 36 3483 NcoI C'CATG_G Cuts at: 0 2640 3519 Size: 2640 879 NdeI CA'TA_TG Cuts at: 0 1107 3519 Size: 1107 2412 NruI TCG'CGA Cuts at: 0 3115 3519 Size: 3115 404 NspI r_CATG'y Cuts at: 0 351 1903 2700 2901 3450 3519 Size: 351 1552 797 201 549 69 Fragments arranged by size: 1552 797 549 351 201 69 PciI A'CATG_T Cuts at: 0 2696 2897 3519 Size: 2696 201 622 Fragments arranged by size: 2696 622 201 PflMI CCAn_nnn'nTGG Cuts at: 0 315 3519 Size: 315 3204 PfoI T'CCnGG_A Cuts at: 0 750 1961 2183 2668 3519 Size: 750 1211 222 485 851 Fragments arranged by size: 1211 851 750 485 222 PpuMI rG'GwC_Cy Cuts at: 0 142 1052 3519 Size: 142 910 2467 Fragments arranged by size: 2467 910 142 PspOMI G'GGCC_C Cuts at: 0 447 3519 Size: 447 3072 PstI C_TGCA'G Cuts at: 0 702 775 1370 1697 3519 Size: 702 73 595 327 1822 Fragments arranged by size: 1822 702 595 327 73 PvuII CAG'CTG Cuts at: 0 480 2169 3096 3519 Size: 480 1689 927 423 Fragments arranged by size: 1689 927 480 423 SacI G_AGCT'C Cuts at: 0 1265 1517 1970 3519 Size: 1265 252 453 1549 Fragments arranged by size: 1549 1265 453 252 SapI GCTCTTCn'nnn_ Cuts at: 0 458 3519 Size: 458 3061 ScaI AGT'ACT Cuts at: 0 1025 3519 Size: 1025 2494 SfcI C'TryA_G Cuts at: 0 698 771 846 1366 1658 1693 1979 3144 Size: 698 73 75 520 292 35 286 1165 Cuts at: 3144 3285 3519 Size: 141 234 Fragments arranged by size: 1165 698 520 292 286 234 141 75 73 35 SfoI GGC'GCC Cuts at: 0 37 3519 Size: 37 3482 SmaI CCC'GGG Cuts at: 0 2809 3519 Size: 2809 710 SmlI C'TyrA_G Cuts at: 0 1341 2071 3519 Size: 1341 730 1448 Fragments arranged by size: 1448 1341 730 SphI G_CATG'C Cuts at: 0 1903 3450 3519 Size: 1903 1547 69 Fragments arranged by size: 1903 1547 69 StuI AGG'CCT Cuts at: 0 472 1190 2095 3519 Size: 472 718 905 1424 Fragments arranged by size: 1424 905 718 472 StyI C'CwwG_G Cuts at: 0 2234 2640 3519 Size: 2234 406 879 Fragments arranged by size: 2234 879 406 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 932 1766 3519 Size: 932 834 1753 Fragments arranged by size: 1753 932 834 TatI w'GTAC_w Cuts at: 0 1023 1705 2332 3519 Size: 1023 682 627 1187 Fragments arranged by size: 1187 1023 682 627 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 411 443 3519 Size: 411 32 3076 Fragments arranged by size: 3076 411 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2505 2889 3519 Size: 2505 384 630 Fragments arranged by size: 2505 630 384 XmaI C'CCGG_G Cuts at: 0 2807 3519 Size: 2807 712 XmnI GAAnn'nnTTC Cuts at: 0 2932 3519 Size: 2932 587 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflII AflIII AleI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BcgI BglII BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI ClaI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NruI NspI PciI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI ScaI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglI BmgBI BmrI BmtI BpuEI BsiWI BspEI BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI CspCI EagI EciI EcoNI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI NaeI NgoMIV NheI NotI NsiI PacI PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI ZraI