################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AccI AccIII AclI AhdI AocI Aor51HI AscI AseI AsnI AspEI AspI AvaI AvaII AvrII BamHI BbvCI BcgI BciVI BcnI BfaI BglII BlnI BsaI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BspHI BspLU11I BspMI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoT22I FseI HpaI KspI MaeI MfeI MluI MroI MunI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmeI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 2134 GACGTC GACGT^C Acc65I 2244 GGTACC G^GTACC AflII 1920 CTTAAG C^TTAAG AflIII 1010 ACGTGT A^C[GA][CT]GT Alw44I 55 GTGCAC G^TGCAC AlwI 1061 GATCC GGATC(4/5) ApaI 207 GGGCCC GGGCC^C ApaLI 55 GTGCAC G^TGCAC Asp718I 2244 GGTACC G^GTACC AviII 1887 TGCGCA TGC^GCA BbeI 148 GGCGCC GGCGC^C BbrPI 1009 CACGTG CAC^GTG BfrI 1920 CTTAAG C^TTAAG BglI 134 GCCGCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1335 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1335 GCTGAGC GC^T[ACGT]AGC BsaAI 1009 CACGTG [CT]AC^GT[GA] BsaBI 282 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 461 TGTACA T^GTACA BspDI 1252 ATCGAT AT^CGAT BsrDI 1555 GCAATG GCAATG(2/0) BsrGI 461 TGTACA T^GTACA BssSI 13 CACGAG CACGAG(-5/-1) BstEII 1115 GGTTACC G^GT[ACGT]ACC BstPI 1115 GGTTACC G^GT[ACGT]ACC BstYI 1060 AGATCC [GA]^GATC[CT] CelII 1335 GCTGAGC GC^T[ACGT]AGC ClaI 1252 ATCGAT AT^CGAT EcoO65I 1115 GGTTACC G^GT[ACGT]ACC EcoRV 1255 GATATC GAT^ATC FauI 1366 GCGGG CCCGC(4/6) FspI 1887 TGCGCA TGC^GCA HaeII 148 GGCGCC [GA]GCGC^[CT] HincII 2110 GTTGAC GT[CT]^[GA]AC HindII 2110 GTTGAC GT[CT]^[GA]AC KasI 148 GGCGCC G^GCGCC KpnI 2244 GGTACC GGTAC^C MamI 282 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1060 AGATCC [GA]^GATC[CT] NarI 148 GGCGCC GG^CGCC NcoI 1307 CCATGG C^CATGG PflMI 2163 CCATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1009 CACGTG CAC^GTG PmlI 1009 CACGTG CAC^GTG PshAI 1975 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2205 GCTCTTC GCTCTTC(1/4) SfoI 148 GGCGCC GGC^GCC SspBI 461 TGTACA T^GTACA StuI 1916 AGGCCT AGG^CCT Van91I 2163 CCATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1060 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AgeI 1841 ACCGGT A^CCGGT 2192 ACCGGT Asp700I 637 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1483 GAACGATTTC BanI 148 GGCGCC G^G[CT][GA]CC 2244 GGTACC BclI 1530 TGATCA T^GATCA 1739 TGATCA BseRI 820 CTCCTC GAGGAG(10/8) 595 GAGGAG BsmI 1362 GAATGC GAATGC(1/-1) 1086 GCATTC BsrBI 908 CCGCTC CCGCTC(-3/-3) 166 GAGCGG BsrFI 1841 ACCGGT [GA]^CCGG[CT] 2192 ACCGGT Cfr10I 1841 ACCGGT [GA]^CCGG[CT] 2192 ACCGGT DraII 206 AGGGCCC [GA]G^G[ACGT]CC[CT] 686 AGGCCCT DsaI 1307 CCATGG C^C[GA][CT]GG 1757 CCGTGG EcoO109I 206 AGGGCCC [GA]G^G[ACGT]CC[CT] 686 AGGCCCT EcoT14I 1307 CCATGG C^C[AT][AT]GG 1832 CCAAGG FbaI 1530 TGATCA T^GATCA 1739 TGATCA HindIII 433 AAGCTT A^AGCTT 1684 AAGCTT MaeII 1010 ACGT A^CGT 2135 ACGT MslI 58 CACGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1728 CACAGAAATG PinAI 1841 ACCGGT A^CCGGT 2192 ACCGGT SacI 961 GAGCTC GAGCT^C 1246 GAGCTC SbfI 969 CCTGCAGG CCTGCA^GG 1812 CCTGCAGG ScaI 479 AGTACT AGT^ACT 1850 AGTACT SmlI 897 CTTGAG C^T[CT][GA]AG 1920 CTTAAG Sse8387I 969 CCTGCAGG CCTGCA^GG 1812 CCTGCAGG StyI 1307 CCATGG C^C[AT][AT]GG 1832 CCAAGG XcmI 1988 CCATGTTCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2003 CCAAGTGCCAGGTGG XmnI 637 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1483 GAACGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwNI ApoI AspHI BalI BanII BbsI BbvI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoNI EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I