(Circular) (Six-base) MAPSORT of: pFN21AE3767.seq Check: 3025 from: 1 to: 6970 pFN21AE3767 6970 bp With 182 enzymes: SgfI * March 28, 2012 12:05 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4143 4780 278 Size: 53 83 186 936 2607 637 2468 Fragments arranged by size: 2607 2468 936 637 186 83 53 AccI GT'mk_AC Cuts at: 1937 4313 1937 Size: 2376 4594 Acc65I G'GTAC_C Cuts at: 4249 4291 4249 Size: 42 6928 AclI AA'CG_TT Cuts at: 1563 5097 5470 1563 Size: 3534 373 3063 Fragments arranged by size: 3534 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2328 2462 2778 3486 3746 4008 5033 6417 Size: 696 134 316 708 260 262 1025 1384 Cuts at: 6417 6685 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1025 708 696 316 268 262 260 134 AflII C'TTAA_G Cuts at: 820 1017 3925 820 Size: 197 2908 3865 Fragments arranged by size: 3865 2908 197 AflIII A'CryG_T Cuts at: 1203 3015 5869 6846 1203 Size: 1812 2854 977 1327 Fragments arranged by size: 2854 1812 1327 977 AgeI A'CCGG_T Cuts at: 3846 4197 5801 3846 Size: 351 1604 5015 Fragments arranged by size: 5015 1604 351 AhdI GACnn_n'nnGTC Cuts at: 5699 5699 Size: 6970 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6970 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6970 AlwNI CAG_nnn'CTG Cuts at: 3464 3724 3826 4234 4713 6285 3464 Size: 260 102 408 479 1572 4149 Fragments arranged by size: 4149 1572 479 408 260 102 ApaI G_GGCC'C Cuts at: 2216 2216 Size: 6970 ApaLI G'TGCA_C Cuts at: 2060 5027 6183 2060 Size: 2967 1156 2847 Fragments arranged by size: 2967 2847 1156 ApoI r'AATT_y Cuts at: 1493 2275 2642 2738 4279 4505 4657 6766 1493 Size: 782 367 96 1541 226 152 2109 1697 Fragments arranged by size: 2109 1697 1541 782 367 226 152 96 AseI AT'TA_AT Cuts at: 160 1032 5524 160 Size: 872 4492 1606 Fragments arranged by size: 4492 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 AvaI C'yCGr_G Cuts at: 1870 1943 4295 1870 Size: 73 2352 4545 Fragments arranged by size: 4545 2352 73 BamHI G'GATC_C Cuts at: 4300 4667 4300 Size: 367 6603 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 4249 4291 5746 618 Size: 325 217 627 366 2096 42 1455 1842 Fragments arranged by size: 2096 1842 1455 627 366 325 217 42 BanII G_rGCy'C Cuts at: 1391 2216 2970 3255 3954 4289 1391 Size: 825 754 285 699 335 4072 Fragments arranged by size: 4072 825 754 699 335 285 BbeI G_GCGC'C Cuts at: 2157 2157 Size: 6970 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3188 3923 4187 928 Size: 971 1289 735 264 3711 Fragments arranged by size: 3711 1289 971 735 264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5161 5195 1903 Size: 34 3224 34 3678 Fragments arranged by size: 3678 3224 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4862 6072 4862 Size: 1210 5760 BclI T'GATC_A Cuts at: 1548 3535 3744 1548 Size: 1987 209 4774 Fragments arranged by size: 4774 1987 209 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2145 5581 136 Size: 107 122 71 631 605 473 3436 1525 Fragments arranged by size: 3436 1525 631 605 473 122 107 71 BglII A'GATC_T Cuts at: 6965 6965 Size: 6970 BlpI GC'TnA_GC Cuts at: 3341 4387 3341 Size: 1046 5924 Bme1580I G_kGCm'C Cuts at: 1790 2064 2216 5031 6187 1790 Size: 274 152 2815 1156 2573 Fragments arranged by size: 2815 2573 1156 274 152 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6970 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2098 3511 4701 Size: 416 53 83 399 24 681 1413 1190 Cuts at: 4701 5659 442 Size: 958 1753 Fragments arranged by size: 1753 1413 1190 958 681 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6970 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4255 4255 Size: 6970 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2685 3008 3107 3310 3377 4085 5630 1239 Size: 1446 323 99 203 67 708 1545 2579 Fragments arranged by size: 2579 1545 1446 708 323 203 99 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2923 5095 5960 6258 2923 Size: 2172 865 298 3635 Fragments arranged by size: 3635 2172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5633 882 Size: 617 4134 2219 Fragments arranged by size: 4134 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3016 4761 493 Size: 711 1812 1745 2702 Fragments arranged by size: 2702 1812 1745 711 BsaBI GATnn'nnATC Cuts at: 2291 6964 2291 Size: 4673 2297 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2096 2154 4140 4777 Size: 53 83 186 936 563 58 1986 637 Cuts at: 4777 5159 5794 275 Size: 382 635 1451 Fragments arranged by size: 1986 1451 936 637 635 563 382 186 83 58 53 BsaWI w'CCGG_w Cuts at: 2855 3846 4197 5403 5801 6075 6222 6633 2855 Size: 991 351 1206 398 274 147 411 3192 Fragments arranged by size: 3192 1206 991 411 398 351 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2808 2838 3119 3149 3833 Size: 30 165 30 1405 30 281 30 684 Cuts at: 3833 3863 1178 Size: 30 4285 Fragments arranged by size: 4285 1405 684 281 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2615 2816 1186 Size: 1429 201 5340 Fragments arranged by size: 5340 1429 201 BseYI C'CCAG_C Cuts at: 3052 3176 6173 6669 3052 Size: 124 2997 496 3353 Fragments arranged by size: 3353 2997 496 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2303 2918 3212 3398 1163 Size: 573 567 615 294 186 4735 Fragments arranged by size: 4735 615 573 567 294 186 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4420 5181 6209 1540 Size: 275 186 2419 761 1028 2301 Fragments arranged by size: 2419 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 2970 3255 5031 5116 6187 2064 Size: 906 285 1776 85 1071 2847 Fragments arranged by size: 2847 1776 1071 906 285 85 BsmI GAATG_Cn' Cuts at: 3091 3373 4481 4574 3091 Size: 282 1108 93 5487 Fragments arranged by size: 5487 1108 282 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2216 2970 3255 3954 4289 5031 Size: 399 274 152 754 285 699 335 742 Cuts at: 5031 5116 6187 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 754 742 699 399 335 285 274 152 85 BspEI T'CCGG_A Cuts at: 6633 6633 Size: 6970 BspHI T'CATG_A Cuts at: 4859 6589 4859 Size: 1730 5240 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4325 844 Size: 3481 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2173 2915 4857 1394 Size: 429 350 742 1942 3507 Fragments arranged by size: 3507 1942 742 429 350 BsrDI GCAATG_nn' Cuts at: 65 1248 3567 5465 5639 65 Size: 1183 2319 1898 174 1396 Fragments arranged by size: 2319 1898 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3846 4197 5614 5801 1700 Size: 110 2036 351 1417 187 2869 Fragments arranged by size: 2869 2036 1417 351 187 110 BsrGI T'GTAC_A Cuts at: 96 2466 96 Size: 2370 4600 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6970 BssSI C'ACGA_G Cuts at: 2018 5030 6042 2018 Size: 3012 1012 2946 Fragments arranged by size: 3012 2946 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6970 BstBI TT'CG_AA Cuts at: 5774 5774 Size: 6970 BstEII G'GTnAC_C Cuts at: 1184 3120 1184 Size: 1936 5034 BstYI r'GATC_y Cuts at: 1972 3065 4300 4667 5052 5069 6521 6607 6619 Size: 1093 1235 367 385 17 1452 86 12 Cuts at: 6619 6965 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1235 1093 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3312 3762 513 Size: 552 939 1308 450 3721 Fragments arranged by size: 3721 1308 939 552 450 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3253 3784 4220 487 Size: 683 56 2027 531 436 3237 Fragments arranged by size: 3237 2027 683 531 436 56 BtsI GCAGTG_nn' Cuts at: 2469 2620 2935 3317 4486 5280 5300 2469 Size: 151 315 382 1169 794 20 4139 Fragments arranged by size: 4139 1169 794 382 315 151 20 ClaI AT'CG_AT Cuts at: 1554 3258 1554 Size: 1704 5266 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6935 DraI TTT'AAA Cuts at: 4274 4274 Size: 6970 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5977 809 Size: 507 4661 1802 Fragments arranged by size: 4661 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2893 3282 Size: 54 1425 50 275 18 177 886 389 Cuts at: 3282 3301 3942 4005 4417 5306 6935 8 Size: 19 641 63 412 889 1629 43 Fragments arranged by size: 1629 1425 889 886 641 412 389 275 177 63 54 50 43 19 18 EagI C'GGCC_G Cuts at: 1537 1812 4417 1537 Size: 275 2605 4090 Fragments arranged by size: 4090 2605 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2247 2592 2877 3056 4217 4900 1353 Size: 894 345 285 179 1161 683 3423 Fragments arranged by size: 3423 1161 894 683 345 285 179 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2191 3486 5557 5931 6077 387 Size: 1064 138 602 1295 2071 374 146 1280 Fragments arranged by size: 2071 1295 1280 1064 602 374 146 138 EcoICRI GAG'CTC Cuts at: 2968 3253 2968 Size: 285 6685 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2328 2462 2685 2778 3008 3107 3310 Size: 393 696 134 223 93 230 99 203 Cuts at: 3310 3377 3486 3746 4008 4085 5033 5630 6417 Size: 67 109 260 262 77 948 597 787 Cuts at: 6417 6685 1239 Size: 268 1524 Fragments arranged by size: 1524 948 787 696 597 393 268 262 260 230 223 203 134 109 99 93 77 67 EcoNI CCTnn'n_nnAGG Cuts at: 2207 2978 3836 2207 Size: 771 858 5341 Fragments arranged by size: 5341 858 771 EcoO109I rG'GnC_Cy Cuts at: 2212 2692 2212 Size: 480 6490 EcoRI G'AATT_C Cuts at: 4279 4657 4279 Size: 378 6592 EcoRV GAT'ATC Cuts at: 3262 3262 Size: 6970 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2594 2594 Size: 6970 FspI TGC'GCA Cuts at: 3894 5476 3894 Size: 1582 5388 HaeII r_GCGC'y Cuts at: 1678 2157 6117 1678 Size: 479 3960 2531 Fragments arranged by size: 3960 2531 479 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2591 2623 4249 Size: 32 135 32 773 32 214 32 1626 Cuts at: 4249 4281 5611 5643 5685 5717 1373 Size: 32 1330 32 42 32 2626 Fragments arranged by size: 2626 1626 1330 773 214 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4117 4314 4560 669 Size: 988 281 2179 197 246 3079 Fragments arranged by size: 3079 2179 988 281 246 197 HindIII A'AGCT_T Cuts at: 748 2438 3689 748 Size: 1690 1251 4029 Fragments arranged by size: 4029 1690 1251 HpaI GTT'AAC Cuts at: 4560 4560 Size: 6970 KasI G'GCGC_C Cuts at: 2153 2153 Size: 6970 KpnI G_GTAC'C Cuts at: 4253 4295 4253 Size: 42 6928 MfeI C'AATT_G Cuts at: 4569 4569 Size: 6970 MluI A'CGCG_T Cuts at: 6846 6846 Size: 6970 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3030 3243 6084 6268 1305 Size: 1725 213 2841 184 2007 Fragments arranged by size: 2841 2007 1725 213 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3944 4007 10 Size: 54 1425 343 177 1935 63 2973 Fragments arranged by size: 2973 1935 1425 343 177 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2067 3737 4928 5287 5446 518 Size: 648 901 1670 1191 359 159 2042 Fragments arranged by size: 2042 1670 1191 901 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2269 4222 4386 5063 6211 6456 1606 Size: 585 78 1953 164 677 1148 245 2120 Fragments arranged by size: 2120 1953 1148 677 585 245 164 78 NarI GG'CG_CC Cuts at: 2154 2154 Size: 6970 NcoI C'CATG_G Cuts at: 513 1065 2004 3312 513 Size: 552 939 1308 4171 Fragments arranged by size: 4171 1308 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6970 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6970 NotI GC'GGCC_GC Cuts at: 4417 4417 Size: 6970 NspI r_CATG'y Cuts at: 1132 1229 4328 5873 1132 Size: 97 3099 1545 2229 Fragments arranged by size: 3099 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3139 4174 1348 Size: 1791 1035 4144 Fragments arranged by size: 4144 1791 1035 PciI A'CATG_T Cuts at: 5869 5869 Size: 6970 PflMI CCAn_nnn'nTGG Cuts at: 1108 4174 1108 Size: 3066 3904 PfoI T'CCnGG_A Cuts at: 3975 3975 Size: 6970 PmeI GTTT'AAAC Cuts at: 4274 4274 Size: 6970 PmlI CAC'GTG Cuts at: 3016 3016 Size: 6970 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5039 5071 6552 6584 5039 Size: 32 1481 32 5425 Fragments arranged by size: 5425 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3984 1613 Size: 2371 4599 PsiI TTA'TAA Cuts at: 4540 4540 Size: 6970 PspOMI G'GGCC_C Cuts at: 2212 2212 Size: 6970 PstI C_TGCA'G Cuts at: 830 2211 2979 3460 3822 4322 830 Size: 1381 768 481 362 500 3478 Fragments arranged by size: 3478 1381 768 500 481 362 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6970 PvuII CAG'CTG Cuts at: 2191 2269 4222 2191 Size: 78 1953 4939 Fragments arranged by size: 4939 1953 78 SacI G_AGCT'C Cuts at: 2970 3255 2970 Size: 285 6685 SalI G'TCGA_C Cuts at: 1936 4312 1936 Size: 2376 4594 SapI GCTCTTCn'nnn_ Cuts at: 4217 4217 Size: 6970 SbfI CC_TGCA'GG Cuts at: 2979 3822 4322 2979 Size: 843 500 5627 Fragments arranged by size: 5627 843 500 ScaI AGT'ACT Cuts at: 2486 3857 5218 6930 2486 Size: 1371 1361 1712 2526 Fragments arranged by size: 2526 1712 1371 1361 SfcI C'TryA_G Cuts at: 826 1045 2207 2431 2975 3456 3818 4318 5453 Size: 219 1162 224 544 481 362 500 1135 Cuts at: 5453 6134 6325 826 Size: 681 191 1471 Fragments arranged by size: 1471 1162 1135 681 544 500 481 362 224 219 191 SfoI GGC'GCC Cuts at: 2155 2155 Size: 6970 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6970 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6970 SmaI CCC'GGG Cuts at: 1872 4297 1872 Size: 2425 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2902 3925 5074 5975 6237 820 Size: 197 926 959 1023 1149 901 262 1553 Fragments arranged by size: 1553 1149 1023 959 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4761 493 Size: 4268 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6970 SphI G_CATG'C Cuts at: 1132 4328 1132 Size: 3196 3774 SspI AAT'ATT Cuts at: 5 52 4894 5 Size: 47 4842 2081 Fragments arranged by size: 4842 2081 47 StuI AGG'CCT Cuts at: 1517 3923 1517 Size: 2406 4564 StyI C'CwwG_G Cuts at: 513 1065 2004 3312 3837 4409 513 Size: 552 939 1308 525 572 3074 Fragments arranged by size: 3074 1308 939 572 552 525 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2080 2936 4997 5014 5167 5352 5797 Size: 1255 500 856 2061 17 153 185 445 Cuts at: 5797 325 Size: 1498 Fragments arranged by size: 2061 1498 1255 856 500 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2466 2484 3109 Size: 274 80 33 51 1443 489 18 625 Cuts at: 3109 3774 3855 5216 6928 96 Size: 665 81 1361 1712 138 Fragments arranged by size: 1712 1443 1361 665 625 489 274 138 81 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2808 2840 3517 3549 4186 4218 6930 6962 2808 Size: 32 677 32 637 32 2712 32 2816 Fragments arranged by size: 2816 2712 677 637 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6970 XbaI T'CTAG_A Cuts at: 4306 4306 Size: 6970 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4000 4015 4000 Size: 15 6955 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6970 XmaI C'CCGG_G Cuts at: 1870 4295 1870 Size: 2425 4545 XmnI GAAnn'nnTTC Cuts at: 2646 3492 5099 2646 Size: 846 1607 4517 Fragments arranged by size: 4517 1607 846 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4141 4778 276 Size: 53 83 186 936 2607 637 2468 Fragments arranged by size: 2607 2468 936 637 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI