(Linear) (Six-base) MAPSORT of: KIEE3767.seq Check: 4238 from: 1 to: 2265 KIEE3767.orf With 182 enzymes: SgfI * March 22, 2012 16:44 .. AatII G_ACGT'C Cuts at: 0 2138 2265 Size: 2138 127 Acc65I G'GTAC_C Cuts at: 0 2244 2265 Size: 2244 21 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 323 457 773 1481 1741 2003 2265 Size: 323 134 316 708 260 262 262 Fragments arranged by size: 708 323 316 262 262 260 134 AflII C'TTAA_G Cuts at: 0 1920 2265 Size: 1920 345 AflIII A'CryG_T Cuts at: 0 1010 2265 Size: 1010 1255 AgeI A'CCGG_T Cuts at: 0 1841 2192 2265 Size: 1841 351 73 Fragments arranged by size: 1841 351 73 AlwNI CAG_nnn'CTG Cuts at: 0 1459 1719 1821 2229 2265 Size: 1459 260 102 408 36 Fragments arranged by size: 1459 408 260 102 36 ApaI G_GGCC'C Cuts at: 0 211 2265 Size: 211 2054 ApaLI G'TGCA_C Cuts at: 0 55 2265 Size: 55 2210 ApoI r'AATT_y Cuts at: 0 270 637 733 2265 Size: 270 367 96 1532 Fragments arranged by size: 1532 367 270 96 BanI G'GyrC_C Cuts at: 0 148 2244 2265 Size: 148 2096 21 Fragments arranged by size: 2096 148 21 BanII G_rGCy'C Cuts at: 0 211 965 1250 1949 2265 Size: 211 754 285 699 316 Fragments arranged by size: 754 699 316 285 211 BbeI G_GCGC'C Cuts at: 0 152 2265 Size: 152 2113 BbsI GAAGACnn'nnnn_ Cuts at: 0 1183 1918 2182 2265 Size: 1183 735 264 83 Fragments arranged by size: 1183 735 264 83 BclI T'GATC_A Cuts at: 0 1530 1739 2265 Size: 1530 209 526 Fragments arranged by size: 1530 526 209 BglI GCCn_nnn'nGGC Cuts at: 0 140 2265 Size: 140 2125 BlpI GC'TnA_GC Cuts at: 0 1336 2265 Size: 1336 929 Bme1580I G_kGCm'C Cuts at: 0 59 211 2265 Size: 59 152 2054 Fragments arranged by size: 2054 152 59 BmrI ACTGGGnnnn_n' Cuts at: 0 93 1506 2265 Size: 93 1413 759 Fragments arranged by size: 1413 759 93 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2250 2265 Size: 2250 15 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 680 1003 1102 1305 1372 2080 2265 Size: 680 323 99 203 67 708 185 Fragments arranged by size: 708 680 323 203 185 99 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 918 2265 Size: 918 1347 BsaAI yAC'GTr Cuts at: 0 1011 2265 Size: 1011 1254 BsaBI GATnn'nnATC Cuts at: 0 286 2265 Size: 286 1979 BsaHI Gr'CG_yC Cuts at: 0 91 149 2135 2265 Size: 91 58 1986 130 Fragments arranged by size: 1986 130 91 58 BsaWI w'CCGG_w Cuts at: 0 850 1841 2192 2265 Size: 850 991 351 73 Fragments arranged by size: 991 850 351 73 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 803 833 1114 1144 1828 1858 2265 Size: 803 30 281 30 684 30 407 Fragments arranged by size: 803 684 407 281 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 610 811 2265 Size: 610 201 1454 Fragments arranged by size: 1454 610 201 BseYI C'CCAG_C Cuts at: 0 1047 1171 2265 Size: 1047 124 1094 Fragments arranged by size: 1094 1047 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 913 1207 1393 2265 Size: 298 615 294 186 872 Fragments arranged by size: 872 615 298 294 186 BsiHKAI G_wGCw'C Cuts at: 0 59 965 1250 2265 Size: 59 906 285 1015 Fragments arranged by size: 1015 906 285 59 BsmI GAATG_Cn' Cuts at: 0 1086 1368 2265 Size: 1086 282 897 Fragments arranged by size: 1086 897 282 Bsp1286I G_dGCh'C Cuts at: 0 59 211 965 1250 1949 2265 Size: 59 152 754 285 699 316 Fragments arranged by size: 754 699 316 285 152 59 BsrBI CCG'CTC Cuts at: 0 168 910 2265 Size: 168 742 1355 Fragments arranged by size: 1355 742 168 BsrDI GCAATG_nn' Cuts at: 0 1562 2265 Size: 1562 703 BsrFI r'CCGG_y Cuts at: 0 1841 2192 2265 Size: 1841 351 73 Fragments arranged by size: 1841 351 73 BsrGI T'GTAC_A Cuts at: 0 461 2265 Size: 461 1804 BssSI C'ACGA_G Cuts at: 0 13 2265 Size: 13 2252 BstEII G'GTnAC_C Cuts at: 0 1115 2265 Size: 1115 1150 BstYI r'GATC_y Cuts at: 0 1060 2265 Size: 1060 1205 BtgI C'CryG_G Cuts at: 0 1307 1757 2265 Size: 1307 450 508 Fragments arranged by size: 1307 508 450 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1248 1779 2215 2265 Size: 1248 531 436 50 Fragments arranged by size: 1248 531 436 50 BtsI GCAGTG_nn' Cuts at: 0 464 615 930 1312 2265 Size: 464 151 315 382 953 Fragments arranged by size: 953 464 382 315 151 ClaI AT'CG_AT Cuts at: 0 1253 2265 Size: 1253 1012 EaeI y'GGCC_r Cuts at: 0 2 888 1277 1296 1937 2000 2265 Size: 2 886 389 19 641 63 265 Fragments arranged by size: 886 641 389 265 63 19 2 EarI CTCTTCn'nnn_ Cuts at: 0 242 587 872 1051 2212 2265 Size: 242 345 285 179 1161 53 Fragments arranged by size: 1161 345 285 242 179 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 186 1481 2265 Size: 186 1295 784 Fragments arranged by size: 1295 784 186 EcoICRI GAG'CTC Cuts at: 0 963 1248 2265 Size: 963 285 1017 Fragments arranged by size: 1017 963 285 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 323 457 680 773 1003 1102 1305 1372 Size: 323 134 223 93 230 99 203 67 Cuts at: 1372 1481 1741 2003 2080 2265 Size: 109 260 262 77 185 Fragments arranged by size: 323 262 260 230 223 203 185 134 109 99 93 77 67 EcoNI CCTnn'n_nnAGG Cuts at: 0 202 973 1831 2265 Size: 202 771 858 434 Fragments arranged by size: 858 771 434 202 EcoO109I rG'GnC_Cy Cuts at: 0 207 687 2265 Size: 207 480 1578 Fragments arranged by size: 1578 480 207 EcoRV GAT'ATC Cuts at: 0 1257 2265 Size: 1257 1008 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 589 2265 Size: 589 1676 FspI TGC'GCA Cuts at: 0 1889 2265 Size: 1889 376 HaeII r_GCGC'y Cuts at: 0 152 2265 Size: 152 2113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 340 372 586 618 2244 2265 Size: 340 32 214 32 1626 21 Fragments arranged by size: 1626 340 214 32 32 21 HincII GTy'rAC Cuts at: 0 2112 2265 Size: 2112 153 HindIII A'AGCT_T Cuts at: 0 433 1684 2265 Size: 433 1251 581 Fragments arranged by size: 1251 581 433 KasI G'GCGC_C Cuts at: 0 148 2265 Size: 148 2117 KpnI G_GTAC'C Cuts at: 0 2248 2265 Size: 2248 17 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1025 1238 2265 Size: 1025 213 1027 Fragments arranged by size: 1027 1025 213 MscI TGG'CCA Cuts at: 0 4 1939 2002 2265 Size: 4 1935 63 263 Fragments arranged by size: 1935 263 63 4 MslI CAynn'nnrTG Cuts at: 0 62 1732 2265 Size: 62 1670 533 Fragments arranged by size: 1670 533 62 MspA1I CmG'CkG Cuts at: 0 186 264 2217 2265 Size: 186 78 1953 48 Fragments arranged by size: 1953 186 78 48 NarI GG'CG_CC Cuts at: 0 149 2265 Size: 149 2116 NcoI C'CATG_G Cuts at: 0 1307 2265 Size: 1307 958 PasI CC'CwG_GG Cuts at: 0 1134 2169 2265 Size: 1134 1035 96 Fragments arranged by size: 1134 1035 96 PflMI CCAn_nnn'nTGG Cuts at: 0 2169 2265 Size: 2169 96 PfoI T'CCnGG_A Cuts at: 0 1970 2265 Size: 1970 295 PmlI CAC'GTG Cuts at: 0 1011 2265 Size: 1011 1254 PshAI GACnn'nnGTC Cuts at: 0 1979 2265 Size: 1979 286 PspOMI G'GGCC_C Cuts at: 0 207 2265 Size: 207 2058 PstI C_TGCA'G Cuts at: 0 206 974 1455 1817 2265 Size: 206 768 481 362 448 Fragments arranged by size: 768 481 448 362 206 PvuII CAG'CTG Cuts at: 0 186 264 2217 2265 Size: 186 78 1953 48 Fragments arranged by size: 1953 186 78 48 SacI G_AGCT'C Cuts at: 0 965 1250 2265 Size: 965 285 1015 Fragments arranged by size: 1015 965 285 SapI GCTCTTCn'nnn_ Cuts at: 0 2212 2265 Size: 2212 53 SbfI CC_TGCA'GG Cuts at: 0 974 1817 2265 Size: 974 843 448 Fragments arranged by size: 974 843 448 ScaI AGT'ACT Cuts at: 0 481 1852 2265 Size: 481 1371 413 Fragments arranged by size: 1371 481 413 SfcI C'TryA_G Cuts at: 0 202 426 970 1451 1813 2265 Size: 202 224 544 481 362 452 Fragments arranged by size: 544 481 452 362 224 202 SfoI GGC'GCC Cuts at: 0 150 2265 Size: 150 2115 SmlI C'TyrA_G Cuts at: 0 897 1920 2265 Size: 897 1023 345 Fragments arranged by size: 1023 897 345 StuI AGG'CCT Cuts at: 0 1918 2265 Size: 1918 347 StyI C'CwwG_G Cuts at: 0 1307 1832 2265 Size: 1307 525 433 Fragments arranged by size: 1307 525 433 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 75 931 2265 Size: 75 856 1334 Fragments arranged by size: 1334 856 75 TatI w'GTAC_w Cuts at: 0 461 479 1104 1769 1850 2265 Size: 461 18 625 665 81 415 Fragments arranged by size: 665 625 461 415 81 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 803 835 1512 1544 2181 2213 2265 Size: 803 32 677 32 637 32 52 Fragments arranged by size: 803 677 637 52 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1995 2010 2265 Size: 1995 15 255 Fragments arranged by size: 1995 255 15 XmnI GAAnn'nnTTC Cuts at: 0 641 1487 2265 Size: 641 846 778 Fragments arranged by size: 846 778 641 ZraI GAC'GTC Cuts at: 0 2136 2265 Size: 2136 129 Enzymes that do cut: AatII Acc65I AcuI AflII AflIII AgeI AlwNI ApaI ApaLI ApoI BanI BanII BbeI BbsI BclI BglI BlpI Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstYI BtgI BtgZI BtsI ClaI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI PasI PflMI PfoI PmlI PshAI PspOMI PstI PvuII SacI SapI SbfI ScaI SfcI SfoI SmlI StuI StyI TaqII TatI TstI XcmI XmnI ZraI Enzymes that do not cut: AarI AccI AclI AfeI AhdI AleI AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI Bpu10I BsaI BsiEI BsiWI BsmBI BspEI BspHI BspMI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I CspCI DraI DraIII DrdI EagI EcoRI FseI FspAI HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI