Restriction map for
pFN21AE0447
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# NO CUT ENZYMES ( 171 enzymes) #
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Acc65I AccII AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp509I Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 117 GACGTC GACGT^C
AcyI 117 GACGTC G[GA]^CG[CT]C
Asp700I 243 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 88 GAGCAC G[AT]GC[AT]^C
BanI 168 GGCACC G^G[CT][GA]CC
BbvI 511 GCAGC GCAGC(8/12)
BpmI 342 CTCCAG CTGGAG(16/14)
BsaHI 117 GACGTC G[GA]^CG[CT]C
BsaI 453 GGTCTC GGTCTC(1/5)
BsaWI 365 ACCGGA [AT]^CCGG[AT]
BsiHKAI 88 GAGCAC G[AT]GC[AT]^C
BsmAI 454 GTCTC GTCTC(1/5)
BsmFI 134 GTCCC GGGAC(10/14)
DraII 422 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 148 CGGCCA [CT]^GGCC[GA]
EcoNI 207 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 422 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 442 CCAAGG C^C[AT][AT]GG
FauI 165 GCGGG CCCGC(4/6)
HaeII 317 AGCGCC [GA]GCGC^[CT]
HapII 366 CCGG C^CGG
Hin1I 117 GACGTC G[GA]^CG[CT]C
HincII 229 GTCAAC GT[CT]^[GA]AC
HindII 229 GTCAAC GT[CT]^[GA]AC
HpaII 366 CCGG C^CGG
MaeII 118 ACGT A^CGT
MaeIII 505 GTCAC ^GT[ACGT]AC
MseI 272 TTAA T^TAA
MspI 366 CCGG C^CGG
PpuMI 422 GGGTCCT [GA]G^G[AT]CC[CT]
SapI 313 GAAGAGC GCTCTTC(1/4)
SfaNI 201 GATGC GCATC(5/9)
StyI 442 CCAAGG C^C[AT][AT]GG
Tru9I 272 TTAA T^TAA
TseI 511 GCAGC G^C[AT]GC
Tsp45I 505 GTCAC ^GT[GC]AC
XcmI 321 CCAGGATGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 243 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 120 GTCTAC GT^[AC][GT]AC
382 GTAGAC
AluI 175 AGCT AG^CT
375 AGCT
AvaII 423 GGTCC G^G[AT]CC
439 GGACC
BamHI 26 GGATCC G^GATCC
368 GGATCC
BmyI 88 GAGCAC G[AGT]GC[ACT]^C
167 GGGCAC
BsiYI 158 CCTTCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
207 CCTGGAAAAGG
BslI 158 CCTTCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
207 CCTGGAAAAGG
Bsp1286I 88 GAGCAC G[AGT]GC[ACT]^C
167 GGGCAC
BsrI 187 ACTGG ACTGG(1/-1)
75 CCAGT
BstYI 26 GGATCC [GA]^GATC[CT]
368 GGATCC
Cac8I 165 GCGGGC GC[ACGT]^[ACGT]GC
498 GCCTGC
EarI 313 GAAGAG CTCTTC(1/4)
490 GAAGAG
HaeIII 149 GGCC GG^CC
460 GGCC
Ksp632I 313 GAAGAG CTCTTC(1/4)
490 GAAGAG
MflI 26 GGATCC [GA]^GATC[CT]
368 GGATCC
MspA1I 174 CAGCTG C[AC]G^C[GT]G
374 CAGCTG
MwoI 461 GCCCTGAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
502 GCTGTCACCGC
PvuII 174 CAGCTG CAG^CTG
374 CAGCTG
TspRI 186 CACTG CA[GC]TG[ACGT][ACGT]^
429 CACTG
XhoII 26 GGATCC [GA]^GATC[CT]
368 GGATCC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AlwI BsaJI BssKI BstF5I
BstNI CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HhaI HinP1I
HinfI HphI ItaI MboI MboII
MnlI MvaI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI