Restriction map for
pFN21AE3935
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII AccII AccIII AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FseI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KspI MamI MfeI MflI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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Acc65I 495 GGTACC G^GTACC
AccI 163 GTCTAC GT^[AC][GT]AC
AclI 330 AACGTT AA^CGTT
Asp718I 495 GGTACC G^GTACC
AviII 1106 TGCGCA TGC^GCA
BanI 495 GGTACC G^G[CT][GA]CC
BbvCI 889 CCTCAGC CCTCAGC(-5/-2)
BciVI 806 GGATAC GTATCC(6/5)
BclI 522 TGATCA T^GATCA
BfaI 17 CTAG C^TAG
BsgI 1351 GTGCAG GTGCAG(16/14)
BsiEI 1263 CGACCG CG[GA][CT]^CG
BspHI 1044 TCATGA T^CATGA
BstF5I 675 GGATG GGATG(2/0)
DraIII 779 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 385 GATATC GAT^ATC
EcoT14I 123 CCATGG C^C[AT][AT]GG
FbaI 522 TGATCA T^GATCA
FokI 675 GGATG GGATG(9/13)
FspI 1106 TGCGCA TGC^GCA
KpnI 495 GGTACC GGTAC^C
MaeI 17 CTAG C^TAG
MslI 235 CATGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 892 CAGCGG C[AC]G^C[GT]G
NcoI 123 CCATGG C^CATGG
NspI 83 ACATGC [GA]CATG^[CT]
Psp1406I 330 AACGTT AA^CGTT
PstI 704 CTGCAG CTGCA^G
RcaI 1044 TCATGA T^CATGA
SfcI 704 CTGCAG C^T[GA][CT]AG
SspI 925 AATATT AAT^ATT
StyI 123 CCATGG C^C[AT][AT]GG
XcmI 1020 CCAACTCTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AciI 36 GCGG CCGC(-3/-1)
894 GCGG
AcsI 40 GAATTT [GA]^AATT[CT]
367 AAATTC
AlwI 589 GATCC GGATC(4/5)
897 GGATC
ApoI 40 GAATTT [GA]^AATT[CT]
367 AAATTC
AvaI 1244 CTCGAG C^[CT]CG[GA]G
1301 CTCGAG
BbsI 730 GAAGAC GAAGAC(2/6)
1239 GAAGAC
BbvI 87 GCTGC GCAGC(8/12)
1249 GCTGC
BpmI 695 CTCCAG CTGGAG(16/14)
1292 CTGGAG
BpuAI 730 GAAGAC GAAGAC(2/6)
1239 GAAGAC
BsmAI 48 GAGAC GTCTC(1/5)
1002 GAGAC
BsoBI 1244 CTCGAG C^[CT]CG[GA]G
1301 CTCGAG
BspMI 702 ACCTGC ACCTGC(4/8)
1037 ACCTGC
Cac8I 150 GCCAGC GC[ACGT]^[ACGT]GC
1383 GCTTGC
CfoI 1107 GCGC GCG^C
1387 GCGC
FauI 36 GCGGG CCCGC(4/6)
894 GCGGG
Fnu4HI 87 GCTGC GC^[ACGT]GC
1249 GCTGC
HhaI 1107 GCGC GCG^C
1387 GCGC
HinP1I 1107 GCGC G^CGC
1387 GCGC
ItaI 87 GCTGC GC^[ACGT]GC
1249 GCTGC
MaeII 331 ACGT A^CGT
1094 ACGT
MwoI 27 GCTGGTCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
706 GCAGACATAGC
PaeR7I 1244 CTCGAG C^TCGAG
1301 CTCGAG
PpuMI 942 GGGACCC [GA]G^G[AT]CC[CT]
1287 GGGACCT
TseI 87 GCTGC G^C[AT]GC
1249 GCTGC
XhoI 1244 CTCGAG C^TCGAG
1301 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AfaI AluI AlwNI AocI AvaII
BsaJI BseRI BsiYI BslI BsmFI
BsrI BssKI BstNI Bsu36I Cfr13I
DdeI DpnII DraII DsaI EarI
Eco81I EcoO109I EcoRII HaeIII HinfI
HphI Ksp632I MaeIII MboI MboII
MnlI MseI MvaI NdeII NlaIII
NlaIV PflMI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I Van91I