Restriction map for pFN21AE3935
 (Linear) (Six-base) MAPSORT of: KIEE3935.seq  Check: 6197  from: 1  to: 1398



KIEE3935.orf

 With 182 enzymes: SgfI * 

                        November 18, 2016 17:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     711    1398 
   Size:        711     687
 
AccI GT'mk_AC

Cuts at:      0     164    1398 
   Size:        164    1234
 
Acc65I G'GTAC_C

Cuts at:      0     495    1398 
   Size:        495     903
 
AclI AA'CG_TT

Cuts at:      0     331    1398 
   Size:        331    1067
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     375     467     488    1398 
   Size:        375      92      21     910

  Fragments arranged by size: 

                 910     375      92      21
 
AlwNI CAG_nnn'CTG

Cuts at:      0     586     622     703     802    1398 
   Size:        586      36      81      99     596

  Fragments arranged by size: 

                 596     586      99      81      36
 
ApoI r'AATT_y

Cuts at:      0      40     367    1398 
   Size:         40     327    1031

  Fragments arranged by size: 

                1031     327      40
 
AvaI C'yCGr_G

Cuts at:      0    1244    1301    1398 
   Size:       1244      57      97

  Fragments arranged by size: 

                1244      97      57
 
BanI G'GyrC_C

Cuts at:      0     495    1398 
   Size:        495     903
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     737    1246    1398 
   Size:        737     509     152

  Fragments arranged by size: 

                 737     509     152
 
BbvCI CC'TCA_GC

Cuts at:      0     890    1398 
   Size:        890     508
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     800    1398 
   Size:        800     598
 
BclI T'GATC_A

Cuts at:      0     522    1398 
   Size:        522     876
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     776     812    1398 
   Size:        776      36     586

  Fragments arranged by size: 

                 776     586      36
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     710    1349    1398 
   Size:        710     639      49

  Fragments arranged by size: 

                 710     639      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     680    1313    1398 
   Size:        680     633      85

  Fragments arranged by size: 

                 680     633      85
 
Bpu10I CC'TnA_GC

Cuts at:      0     644     890    1183    1398 
   Size:        644     246     293     215

  Fragments arranged by size: 

                 644     293     246     215
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     756    1398 
   Size:        756     642
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1334    1364    1398 
   Size:       1334      30      34

  Fragments arranged by size: 

                1334      34      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     330     454     577    1398 
   Size:        330     124     123     821

  Fragments arranged by size: 

                 821     330     124     123
 
BseYI C'CCAG_C

Cuts at:      0    1311    1398 
   Size:       1311      87
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1372    1398 
   Size:       1372      26
 
BsiEI CG_ry'CG

Cuts at:      0    1266    1398 
   Size:       1266     132
 
BspHI T'CATG_A

Cuts at:      0    1044    1398 
   Size:       1044     354
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     711    1046    1398 
   Size:        711     335     352

  Fragments arranged by size: 

                 711     352     335
 
Bsu36I CC'TnA_GG

Cuts at:      0     347     434     488     671    1398 
   Size:        347      87      54     183     727

  Fragments arranged by size: 

                 727     347     183      87      54
 
BtgI C'CryG_G

Cuts at:      0     123    1338    1390    1398 
   Size:        123    1215      52       8

  Fragments arranged by size: 

                1215     123      52       8
 
BtsI GCAGTG_nn'

Cuts at:      0      61    1398 
   Size:         61    1337
 
DraIII CAC_nnn'GTG

Cuts at:      0     784    1398 
   Size:        784     614
 
EarI CTCTTCn'nnn_

Cuts at:      0     117     679    1030    1226    1398 
   Size:        117     562     351     196     172

  Fragments arranged by size: 

                 562     351     196     172     117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     375     467     488     680    1313    1398 
   Size:        375      92      21     192     633      85

  Fragments arranged by size: 

                 633     375     192      92      85      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75     943    1288    1297    1330    1398 
   Size:         75     868     345       9      33      68

  Fragments arranged by size: 

                 868     345      75      68      33       9
 
EcoRV GAT'ATC

Cuts at:      0     387    1398 
   Size:        387    1011
 
FspI TGC'GCA

Cuts at:      0    1108    1398 
   Size:       1108     290
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     798     830    1199    1231    1398 
   Size:        798      32     369      32     167

  Fragments arranged by size: 

                 798     369     167      32      32
 
KpnI G_GTAC'C

Cuts at:      0     499    1398 
   Size:        499     899
 
MslI CAynn'nnrTG

Cuts at:      0     239    1398 
   Size:        239    1159
 
MspA1I CmG'CkG

Cuts at:      0     894    1398 
   Size:        894     504
 
NcoI C'CATG_G

Cuts at:      0     123    1398 
   Size:        123    1275
 
NspI r_CATG'y

Cuts at:      0      87    1398 
   Size:         87    1311
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     282     784     802    1366    1398 
   Size:        282     502      18     564      32

  Fragments arranged by size: 

                 564     502     282      32      18
 
PpuMI rG'GwC_Cy

Cuts at:      0     943    1288    1398 
   Size:        943     345     110

  Fragments arranged by size: 

                 943     345     110
 
PspXI vC'TCGA_Gb

Cuts at:      0    1244    1301    1398 
   Size:       1244      57      97

  Fragments arranged by size: 

                1244      97      57
 
PstI C_TGCA'G

Cuts at:      0     708    1398 
   Size:        708     690
 
SanDI GG'GwC_CC

Cuts at:      0     943    1398 
   Size:        943     455
 
SfcI C'TryA_G

Cuts at:      0     704    1398 
   Size:        704     694
 
SmlI C'TyrA_G

Cuts at:      0     735    1244    1301    1398 
   Size:        735     509      57      97

  Fragments arranged by size: 

                 735     509      97      57
 
SspI AAT'ATT

Cuts at:      0     927    1398 
   Size:        927     471
 
StyI C'CwwG_G

Cuts at:      0     123    1398 
   Size:        123    1275
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     795    1280    1303    1398 
   Size:        795     485      23      95

  Fragments arranged by size: 

                 795     485      95      23
 
TatI w'GTAC_w

Cuts at:      0     723    1398 
   Size:        723     675
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1082    1114    1334    1355    1366    1387    1398 
   Size:       1082      32     220      21      11      21      11

  Fragments arranged by size: 

                1082     220      32      21      21      11      11
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1027    1398 
   Size:       1027     371
 
XhoI C'TCGA_G

Cuts at:      0    1244    1301    1398 
   Size:       1244      57      97

  Fragments arranged by size: 

                1244      97      57
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AlwNI     ApoI     AvaI
     BanI     BbsI    BbvCI    BciVI     BclI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BspHI
    BspMI   Bsu36I     BtgI     BtsI   DraIII     EarI  Eco57MI EcoO109I
    EcoRV     FspI    Hin4I     KpnI     MslI   MspA1I     NcoI     NspI
    PflMI    PpuMI    PspXI     PstI    SanDI     SfcI     SmlI     SspI
     StyI    TaqII     TatI     TstI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AfeI    AflII   AflIII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BanII     BbeI     BcgI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI  Tth111I     XbaI     XmaI     XmnI     ZraI
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