################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 31 CGCG CG^CG AclI 144 AACGTT AA^CGTT AcsI 224 AAATTT [GA]^AATT[CT] AfaI 688 GTAC GT^AC AflII 985 CTTAAG C^TTAAG AflIII 171 ACATGT A^C[GA][CT]GT ApoI 224 AAATTT [GA]^AATT[CT] Asp700I 487 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 1210 GGACC G^G[AT]CC BalI 1188 TGGCCA TGG^CCA BclI 579 TGATCA T^GATCA BcnI 862 CCGGG CC^[GC]GG BfrI 985 CTTAAG C^TTAAG BpmI 587 CTCCAG CTGGAG(16/14) BsiYI 499 CCAAATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 499 CCAAATACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 573 GTCCC GGGAC(10/14) BsmI 193 GAATGC GAATGC(1/-1) BspLU11I 171 ACATGT A^CATGT BstAPI 937 GCATACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 31 CGCG CG^CG CfoI 886 GCGC GCG^C DraI 228 TTTAAA TTT^AAA DsaI 716 CCGTGG C^C[GA][CT]GG EaeI 1188 TGGCCA [CT]^GGCC[GA] EcoT14I 1053 CCTTGG C^C[AT][AT]GG FbaI 579 TGATCA T^GATCA HapII 862 CCGG C^CGG HhaI 886 GCGC GCG^C HinP1I 886 GCGC G^CGC HincII 1176 GTTAAC GT[CT]^[GA]AC HindII 1176 GTTAAC GT[CT]^[GA]AC HpaI 1176 GTTAAC GTT^AAC HpaII 862 CCGG C^CGG HphI 1266 GGTGA GGTGA(8/7) MluNI 1188 TGGCCA TGG^CCA MscI 1188 TGGCCA TGG^CCA MslI 889 CATACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 862 CCGG C^CGG MvnI 31 CGCG CG^CG NciI 862 CCGGG CC^[GC]GG NruI 30 TCGCGA TCG^CGA NspI 171 ACATGT [GA]CATG^[CT] PflMI 499 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 34 GAGTC GAGTC(4/5) Psp1406I 144 AACGTT AA^CGTT RsaI 688 GTAC GT^AC ScaI 687 AGTACT AGT^ACT SfcI 841 CTACAG C^T[GA][CT]AG StuI 876 AGGCCT AGG^CCT StyI 1053 CCTTGG C^C[AT][AT]GG TaqI 657 TCGA T^CGA TfiI 240 GAATC G^A[AT]TC TthHB8I 657 TCGA T^CGA Van91I 499 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 487 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwI 567 GATCC GGATC(4/5) 859 GATCC AspHI 163 GAGCTC G[AT]GC[AT]^C 670 GAGCTC BanII 163 GAGCTC G[GA]GC[CT]^C 670 GAGCTC BmyI 163 GAGCTC G[AGT]GC[ACT]^C 670 GAGCTC BsiHKAI 163 GAGCTC G[AT]GC[AT]^C 670 GAGCTC Bsp1286I 163 GAGCTC G[AGT]GC[ACT]^C 670 GAGCTC BsrI 505 ACTGG ACTGG(1/-1) 828 CCAGT BstNI 415 CCTGG CC^[AT]GG 1182 CCTGG BstYI 566 AGATCC [GA]^GATC[CT] 858 AGATCC Cfr13I 1057 GGGCC G^G[ACGT]CC 1210 GGACC EcoRII 415 CCTGG ^CC[AT]GG 1182 CCTGG HinfI 34 GAGTC G^A[ACGT]TC 240 GAATC MflI 566 AGATCC [GA]^GATC[CT] 858 AGATCC MvaI 415 CCTGG CC^[AT]GG 1182 CCTGG MwoI 937 GCATACCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1123 GCTCCAACAGC SacI 163 GAGCTC GAGCT^C 670 GAGCTC Sau96I 1057 GGGCC G^G[ACGT]CC 1210 GGACC SfaNI 509 GATGC GCATC(5/9) 990 GCATC SmlI 38 CTCAAG C^T[CT][GA]AG 985 CTTAAG Tsp45I 454 GTGAC ^GT[GC]AC 1162 GTGAC XbaI 369 TCTAGA T^CTAGA 1236 TCTAGA XhoII 566 AGATCC [GA]^GATC[CT] 858 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AluI BbvI BfaI BsaJI BsmAI BssKI BstF5I DdeI DpnII Fnu4HI FokI HaeIII ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I NdeII NlaIII PvuII Sau3AI ScrFI SspI Tru9I TseI Tsp509I TspRI