(Circular) (Six-base) MAPSORT of: pFN21AE3927.seq Check: 9807 from: 1 to: 6010 pFN21AE3927 6010 bp With 182 enzymes: SgfI * March 28, 2012 11:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3820 278 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3353 1937 Size: 1416 4594 Acc65I G'GTAC_C Cuts at: 3331 3331 Size: 6010 AclI AA'CG_TT Cuts at: 1563 2150 4137 4510 1563 Size: 587 1987 373 3063 Fragments arranged by size: 3063 1987 587 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2244 3158 4073 5457 5725 1632 Size: 612 914 915 1384 268 1917 Fragments arranged by size: 1917 1384 915 914 612 268 AflII C'TTAA_G Cuts at: 820 1017 2990 820 Size: 197 1973 3840 Fragments arranged by size: 3840 1973 197 AflIII A'CryG_T Cuts at: 1203 2176 4909 5886 1203 Size: 973 2733 977 1327 Fragments arranged by size: 2733 1327 977 973 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6010 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6010 AlwNI CAG_nnn'CTG Cuts at: 3753 5325 3753 Size: 1572 4438 ApaLI G'TGCA_C Cuts at: 4067 5223 4067 Size: 1156 4854 ApoI r'AATT_y Cuts at: 1493 2229 3319 3545 3697 5806 1493 Size: 736 1090 226 152 2109 1697 Fragments arranged by size: 2109 1697 1090 736 226 152 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 3335 1870 Size: 73 1392 4545 Fragments arranged by size: 4545 1392 73 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3331 4786 618 Size: 325 217 627 1544 1455 1842 Fragments arranged by size: 1842 1544 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2172 2679 3329 1391 Size: 781 507 650 4072 Fragments arranged by size: 4072 781 650 507 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5039 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4201 4235 1903 Size: 34 50 34 2180 34 3678 Fragments arranged by size: 3678 2180 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3902 5112 3902 Size: 1210 4800 BclI T'GATC_A Cuts at: 1548 2584 1548 Size: 1036 4974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4621 136 Size: 107 122 71 631 605 2949 1525 Fragments arranged by size: 2949 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6005 6005 Size: 6010 BlpI GC'TnA_GC Cuts at: 3427 3427 Size: 6010 Bme1580I G_kGCm'C Cuts at: 1790 4071 5227 1790 Size: 2281 1156 2573 Fragments arranged by size: 2573 2281 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2827 3741 4699 Size: 416 53 83 399 24 1410 914 958 Cuts at: 4699 442 Size: 1753 Fragments arranged by size: 1753 1410 958 914 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2577 4670 1239 Size: 1338 2093 2579 Fragments arranged by size: 2579 2093 1338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2028 4135 5000 5298 2028 Size: 2107 865 298 2740 Fragments arranged by size: 2740 2107 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4673 882 Size: 617 3174 2219 Fragments arranged by size: 3174 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3801 493 Size: 711 2597 2702 Fragments arranged by size: 2702 2597 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3817 4199 4834 275 Size: 53 83 186 936 2284 382 635 1451 Fragments arranged by size: 2284 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4443 4841 5115 5262 5673 4443 Size: 398 274 147 411 4780 Fragments arranged by size: 4780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5785 Fragments arranged by size: 5785 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6010 BseYI C'CCAG_C Cuts at: 5213 5709 5213 Size: 496 5514 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5437 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3460 4221 5249 1540 Size: 275 186 1459 761 1028 2301 Fragments arranged by size: 2301 1459 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2172 2679 4071 4156 5227 2172 Size: 507 1392 85 1071 2955 Fragments arranged by size: 2955 1392 1071 507 85 BsmI GAATG_Cn' Cuts at: 2204 3521 3614 2204 Size: 1317 93 4600 Fragments arranged by size: 4600 1317 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2679 3329 4071 4156 5227 1391 Size: 399 382 507 650 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 650 507 399 382 85 BspEI T'CCGG_A Cuts at: 5673 5673 Size: 6010 BspHI T'CATG_A Cuts at: 3899 5629 3899 Size: 1730 4280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3365 844 Size: 2521 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3897 1394 Size: 429 2074 3507 Fragments arranged by size: 3507 2074 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4505 4679 65 Size: 1183 3257 174 1396 Fragments arranged by size: 3257 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4654 4841 1700 Size: 110 2844 187 2869 Fragments arranged by size: 2869 2844 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 4070 5082 4070 Size: 1012 4998 BstAPI GCAn_nnn'nTGC Cuts at: 794 2948 794 Size: 2154 3856 BstBI TT'CG_AA Cuts at: 4814 4814 Size: 6010 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstYI r'GATC_y Cuts at: 1972 2571 2863 3340 3707 4092 4109 5561 5647 Size: 599 292 477 367 385 17 1452 86 Cuts at: 5647 5659 6005 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 599 477 385 367 346 292 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2721 513 Size: 552 1656 3802 Fragments arranged by size: 3802 1656 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5271 Fragments arranged by size: 5271 683 56 BtsI GCAGTG_nn' Cuts at: 3526 4320 4340 3526 Size: 794 20 5196 Fragments arranged by size: 5196 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 2235 3314 2235 Size: 1079 4931 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5017 809 Size: 507 3701 1802 Fragments arranged by size: 3701 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3193 3457 4346 Size: 54 1425 50 275 18 1363 264 889 Cuts at: 4346 5975 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1363 889 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3457 1537 Size: 275 1645 4090 Fragments arranged by size: 4090 1645 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3940 1353 Size: 2587 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4597 4971 5117 387 Size: 1064 138 3008 374 146 1280 Fragments arranged by size: 3008 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2170 2677 2170 Size: 507 5503 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2244 2577 3158 4073 4670 5457 5725 Size: 393 612 333 581 915 597 787 268 Cuts at: 5725 1239 Size: 1524 Fragments arranged by size: 1524 915 787 612 597 581 393 333 268 EcoRI G'AATT_C Cuts at: 3319 3697 3319 Size: 378 5632 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2429 2429 Size: 6010 FspI TGC'GCA Cuts at: 4516 4516 Size: 6010 HaeII r_GCGC'y Cuts at: 1678 5157 1678 Size: 3479 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2794 2826 3034 3066 3145 Size: 32 135 32 1222 32 208 32 79 Cuts at: 3145 3177 4651 4683 4725 4757 1373 Size: 32 1474 32 42 32 2626 Fragments arranged by size: 2626 1474 1222 208 135 79 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3183 3354 3600 669 Size: 988 281 1245 171 246 3079 Fragments arranged by size: 3079 1245 988 281 246 171 HindIII A'AGCT_T Cuts at: 748 748 Size: 6010 HpaI GTT'AAC Cuts at: 3183 3600 3183 Size: 417 5593 KpnI G_GTAC'C Cuts at: 3335 3335 Size: 6010 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3155 5124 5308 1305 Size: 1850 1969 184 2007 Fragments arranged by size: 2007 1969 1850 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3195 10 Size: 54 1425 343 1363 2825 Fragments arranged by size: 2825 1425 1363 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2898 3968 4327 4486 518 Size: 648 1732 1070 359 159 2042 Fragments arranged by size: 2042 1732 1070 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2339 2606 3178 3426 4103 5251 5496 1606 Size: 733 267 572 248 677 1148 245 2120 Fragments arranged by size: 2120 1148 733 677 572 267 248 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5458 NdeI CA'TA_TG Cuts at: 387 387 Size: 6010 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NruI TCG'CGA Cuts at: 2037 2037 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 2180 3368 4913 1132 Size: 97 951 1188 1545 2229 Fragments arranged by size: 2229 1545 1188 951 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6010 PciI A'CATG_T Cuts at: 2176 4909 2176 Size: 2733 3277 PflMI CCAn_nnn'nTGG Cuts at: 1108 2510 1108 Size: 1402 4608 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2355 2387 2355 Size: 32 5978 PstI C_TGCA'G Cuts at: 830 3362 830 Size: 2532 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2339 2606 3178 2339 Size: 267 572 5171 Fragments arranged by size: 5171 572 267 SacI G_AGCT'C Cuts at: 2172 2679 2172 Size: 507 5503 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SbfI CC_TGCA'GG Cuts at: 3362 3362 Size: 6010 ScaI AGT'ACT Cuts at: 2694 4258 5970 2694 Size: 1564 1712 2734 Fragments arranged by size: 2734 1712 1564 SfcI C'TryA_G Cuts at: 826 1045 2846 3358 4493 5174 5365 826 Size: 219 1801 512 1135 681 191 1471 Fragments arranged by size: 1801 1471 1135 681 512 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6010 SmaI CCC'GGG Cuts at: 1872 3337 1872 Size: 1465 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2043 2990 4114 5015 5277 820 Size: 197 926 100 947 1124 901 262 1553 Fragments arranged by size: 1553 1124 947 926 901 262 197 100 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 2711 2773 3099 3142 3934 5 Size: 47 2659 62 326 43 792 2081 Fragments arranged by size: 2659 2081 792 326 62 47 43 StuI AGG'CCT Cuts at: 1517 2883 1517 Size: 1366 4644 StyI C'CwwG_G Cuts at: 513 1065 3058 3449 513 Size: 552 1993 391 3074 Fragments arranged by size: 3074 1993 552 391 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4037 4054 4207 4392 4837 325 Size: 1255 2457 17 153 185 445 1498 Fragments arranged by size: 2457 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2692 4256 5968 Size: 274 80 33 51 1443 715 1564 1712 Cuts at: 5968 96 Size: 138 Fragments arranged by size: 1712 1564 1443 715 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5970 6002 5970 Size: 32 5978 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6010 XbaI T'CTAG_A Cuts at: 2374 3241 3346 2374 Size: 867 105 5038 Fragments arranged by size: 5038 867 105 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6010 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6010 XmaI C'CCGG_G Cuts at: 1870 3335 1870 Size: 1465 4545 XmnI GAAnn'nnTTC Cuts at: 2496 4139 2496 Size: 1643 4367 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3818 276 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI