Restriction map for
pFN21AE0439
(Circular) (Six-base) MAPSORT of: pFN21AE0439.seq Check: 8528 from: 1 to: 5647
pFN21AE0439 5647 bp
With 182 enzymes: SgfI *
October 20, 2009 10:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3457 278
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2172 2598 2990 1937
Size: 235 426 392 4594
Fragments arranged by size:
4594 426 392 235
Acc65I G'GTAC_C
Cuts at: 2968 2968
Size: 5647
AclI AA'CG_TT
Cuts at: 1563 3774 4147 1563
Size: 2211 373 3063
Fragments arranged by size:
3063 2211 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 3710 5094 5362 1632
Size: 764 1314 1384 268 1917
Fragments arranged by size:
1917 1384 1314 764 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5450
AflIII A'CryG_T
Cuts at: 1203 4546 5523 1203
Size: 3343 977 1327
Fragments arranged by size:
3343 1327 977
AgeI A'CCGG_T
Cuts at: 4478 4478
Size: 5647
AhdI GACnn_n'nnGTC
Cuts at: 4376 4376
Size: 5647
AleI CACnn'nnGTG
Cuts at: 1166 2162 1166
Size: 996 4651
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5647
AlwNI CAG_nnn'CTG
Cuts at: 2150 2531 3390 4962 2150
Size: 381 859 1572 2835
Fragments arranged by size:
2835 1572 859 381
ApaI G_GGCC'C
Cuts at: 2777 2777
Size: 5647
ApaLI G'TGCA_C
Cuts at: 3704 4860 3704
Size: 1156 4491
ApoI r'AATT_y
Cuts at: 1493 2276 2956 3182 3334 5443 1493
Size: 783 680 226 152 2109 1697
Fragments arranged by size:
2109 1697 783 680 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4201 160
Size: 872 3169 1606
Fragments arranged by size:
3169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
AvaI C'yCGr_G
Cuts at: 1870 1943 2972 1870
Size: 73 1029 4545
Fragments arranged by size:
4545 1029 73
AvrII C'CTAG_G
Cuts at: 2614 2614
Size: 5647
BamHI G'GATC_C
Cuts at: 2977 3344 2977
Size: 367 5280
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 2968 4423 618
Size: 325 217 627 333 848 1455 1842
Fragments arranged by size:
1842 1455 848 627 333 325 217
BanII G_rGCy'C
Cuts at: 1391 2394 2777 2966 1391
Size: 1003 383 189 4072
Fragments arranged by size:
4072 1003 383 189
BbeI G_GCGC'C
Cuts at: 2124 2124
Size: 5647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2294 2585 2736 2924 928
Size: 971 395 291 151 188 3651
Fragments arranged by size:
3651 971 395 291 188 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3838 3872 1903
Size: 34 1901 34 3678
Fragments arranged by size:
3678 1901 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2800 3539 4749 2800
Size: 739 1210 3698
Fragments arranged by size:
3698 1210 739
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4258 136
Size: 107 122 71 631 605 2586 1525
Fragments arranged by size:
2586 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5642 5642
Size: 5647
BlpI GC'TnA_GC
Cuts at: 3064 3064
Size: 5647
Bme1580I G_kGCm'C
Cuts at: 1790 2777 3708 4864 1790
Size: 987 931 1156 2573
Fragments arranged by size:
2573 1156 987 931
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5647
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2519 3378 4336
Size: 416 53 83 399 24 1102 859 958
Cuts at: 4336 442
Size: 1753
Fragments arranged by size:
1753 1102 958 859 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2943 4307 1239
Size: 1704 1364 2579
Fragments arranged by size:
2579 1704 1364
Bpu10I CC'TnA_GC
Cuts at: 2294 2294
Size: 5647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3772 4637 4935 3772
Size: 865 298 4484
Fragments arranged by size:
4484 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4310 882
Size: 617 2811 2219
Fragments arranged by size:
2811 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2092 3438 493
Size: 711 888 1346 2702
Fragments arranged by size:
2702 1346 888 711
BsaBI GATnn'nnATC
Cuts at: 5641 5641
Size: 5647
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2121 3454 3836 4471
Size: 53 83 186 936 588 1333 382 635
Cuts at: 4471 275
Size: 1451
Fragments arranged by size:
1451 1333 936 635 588 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4080 4478 4752 4899 5310 4080
Size: 398 274 147 411 4417
Fragments arranged by size:
4417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 1178
Size: 30 165 30 1032 30 4360
Fragments arranged by size:
4360 1032 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 1186
Size: 849 4798
BseYI C'CCAG_C
Cuts at: 2870 4850 5346 2870
Size: 1980 496 3171
Fragments arranged by size:
3171 1980 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5074
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3097 3858 4886 1540
Size: 275 186 1096 761 1028 2301
Fragments arranged by size:
2301 1096 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2300 2394 3708 3793 4864 2300
Size: 94 1314 85 1071 3083
Fragments arranged by size:
3083 1314 1071 94 85
BsmI GAATG_Cn'
Cuts at: 3158 3251 3158
Size: 93 5554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2300 2394 2777 2966 3708 3793 4864
Size: 399 510 94 383 189 742 85 1071
Cuts at: 4864 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 510 399 383 189 94
85
BspEI T'CCGG_A
Cuts at: 5310 5310
Size: 5647
BspHI T'CATG_A
Cuts at: 2535 3536 5266 2535
Size: 1001 1730 2916
Fragments arranged by size:
2916 1730 1001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3002 844
Size: 2158 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3534 1394
Size: 429 1711 3507
Fragments arranged by size:
3507 1711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 4142 4316 65
Size: 1183 879 2015 174 1396
Fragments arranged by size:
2015 1396 1183 879 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2123 2267 2759 4291 4478 1700
Size: 110 313 144 492 1532 187 2869
Fragments arranged by size:
2869 1532 492 313 187 144 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5647
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5647
BssSI C'ACGA_G
Cuts at: 3707 4719 3707
Size: 1012 4635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2029 794
Size: 1235 4412
BstBI TT'CG_AA
Cuts at: 4451 4451
Size: 5647
BstEII G'GTnAC_C
Cuts at: 1184 2762 1184
Size: 1578 4069
BstYI r'GATC_y
Cuts at: 1972 2977 3344 3729 3746 5198 5284 5296 5642
Size: 1005 367 385 17 1452 86 12 346
Cuts at: 5642 1972
Size: 1977
Fragments arranged by size:
1977 1452 1005 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2225 2262 2225
Size: 37 5610
BtgI C'CryG_G
Cuts at: 513 1065 2004 2769 513
Size: 552 939 765 3391
Fragments arranged by size:
3391 939 765 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2286 487
Size: 683 56 1060 3848
Fragments arranged by size:
3848 1060 683 56
BtsI GCAGTG_nn'
Cuts at: 2739 3163 3957 3977 2739
Size: 424 794 20 4409
Fragments arranged by size:
4409 794 424 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5612
DraI TTT'AAA
Cuts at: 2951 2951
Size: 5647
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4654 809
Size: 507 3338 1802
Fragments arranged by size:
3338 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 3094 3983
Size: 54 1425 50 275 18 237 1027 889
Cuts at: 3983 5612 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1027 889 275 237 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3094 1537
Size: 275 1282 4090
Fragments arranged by size:
4090 1282 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2399 3577 1353
Size: 1046 1178 3423
Fragments arranged by size:
3423 1178 1046
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4234 4608 4754 387
Size: 1064 138 2645 374 146 1280
Fragments arranged by size:
2645 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2392 2392
Size: 5647
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2396 2943 3710 4307 5094 5362 1239
Size: 393 764 547 767 597 787 268 1524
Fragments arranged by size:
1524 787 767 764 597 547 393 268
EcoRI G'AATT_C
Cuts at: 2956 3334 2956
Size: 378 5269
FspI TGC'GCA
Cuts at: 4153 4153
Size: 5647
HaeII r_GCGC'y
Cuts at: 1678 2124 4794 1678
Size: 446 2670 2531
Fragments arranged by size:
2670 2531 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2309 2341 4288 4320 4362
Size: 32 135 32 737 32 1947 32 42
Cuts at: 4362 4394 1373
Size: 32 2626
Fragments arranged by size:
2626 1947 737 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2991 3237 669
Size: 988 281 1053 246 3079
Fragments arranged by size:
3079 1053 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5647
HpaI GTT'AAC
Cuts at: 3237 3237
Size: 5647
KasI G'GCGC_C
Cuts at: 2120 2120
Size: 5647
KpnI G_GTAC'C
Cuts at: 2972 2972
Size: 5647
MfeI C'AATT_G
Cuts at: 3246 3246
Size: 5647
MluI A'CGCG_T
Cuts at: 5523 5523
Size: 5647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2779 4761 4945 1305
Size: 1474 1982 184 2007
Fragments arranged by size:
2007 1982 1474 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3825
Fragments arranged by size:
3825 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 2744 3605 3964 4123 518
Size: 648 996 582 861 359 159 2042
Fragments arranged by size:
2042 996 861 648 582 359 159
MspA1I CmG'CkG
Cuts at: 1606 3063 3740 4888 5133 1606
Size: 1457 677 1148 245 2120
Fragments arranged by size:
2120 1457 1148 677 245
NaeI GCC'GGC
Cuts at: 2125 2269 2125
Size: 144 5503
NarI GG'CG_CC
Cuts at: 2121 2121
Size: 5647
NcoI C'CATG_G
Cuts at: 513 1065 2004 2769 513
Size: 552 939 765 3391
Fragments arranged by size:
3391 939 765 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5647
NgoMIV G'CCGG_C
Cuts at: 2123 2267 2123
Size: 144 5503
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5647
NotI GC'GGCC_GC
Cuts at: 3094 3094
Size: 5647
NspI r_CATG'y
Cuts at: 1132 1229 3005 4550 1132
Size: 97 1776 1545 2229
Fragments arranged by size:
2229 1776 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5647
PciI A'CATG_T
Cuts at: 4546 4546
Size: 5647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5647
PmeI GTTT'AAAC
Cuts at: 2951 2951
Size: 5647
PmlI CAC'GTG
Cuts at: 2092 2092
Size: 5647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 3716 3748 5229 5261 2891
Size: 32 793 32 1481 32 3277
Fragments arranged by size:
3277 1481 793 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5647
PsiI TTA'TAA
Cuts at: 3217 3217
Size: 5647
PspOMI G'GGCC_C
Cuts at: 2773 2773
Size: 5647
PstI C_TGCA'G
Cuts at: 830 2146 2999 830
Size: 1316 853 3478
Fragments arranged by size:
3478 1316 853
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5647
SacI G_AGCT'C
Cuts at: 2394 2394
Size: 5647
SalI G'TCGA_C
Cuts at: 1936 2989 1936
Size: 1053 4594
SapI GCTCTTCn'nnn_
Cuts at: 2399 2399
Size: 5647
SbfI CC_TGCA'GG
Cuts at: 2999 2999
Size: 5647
ScaI AGT'ACT
Cuts at: 3895 5607 3895
Size: 1712 3935
SfcI C'TryA_G
Cuts at: 826 1045 2142 2995 4130 4811 5002 826
Size: 219 1097 853 1135 681 191 1471
Fragments arranged by size:
1471 1135 1097 853 681 219 191
SfoI GGC'GCC
Cuts at: 2122 2122
Size: 5647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5647
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5647
SmaI CCC'GGG
Cuts at: 1872 2974 1872
Size: 1102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3751 4652 4914 820
Size: 197 926 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3438 493
Size: 2945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5647
SphI G_CATG'C
Cuts at: 1132 3005 1132
Size: 1873 3774
SspI AAT'ATT
Cuts at: 5 52 3571 5
Size: 47 3519 2081
Fragments arranged by size:
3519 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5647
StyI C'CwwG_G
Cuts at: 513 1065 2004 2614 2769 3086 513
Size: 552 939 610 155 317 3074
Fragments arranged by size:
3074 939 610 552 317 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2511 2820 3674 3691 3844 4029 4474
Size: 1255 931 309 854 17 153 185 445
Cuts at: 4474 325
Size: 1498
Fragments arranged by size:
1498 1255 931 854 445 309 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3893 5605 96
Size: 274 80 33 51 1443 1916 1712 138
Fragments arranged by size:
1916 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5607 5639 5607
Size: 32 5615
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5647
XbaI T'CTAG_A
Cuts at: 2788 2983 2788
Size: 195 5452
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5647
XmaI C'CCGG_G
Cuts at: 1870 2972 1870
Size: 1102 4545
XmnI GAAnn'nnTTC
Cuts at: 2906 3776 2906
Size: 870 4777
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3455 276
Size: 53 83 186 936 1921 2468
Fragments arranged by size:
2468 1921 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PpuMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SrfI SwaI XcmI