Restriction map for
pFN21AE0439
(Linear) (Six-base) MAPSORT of: KIEE0439.seq Check: 3585 from: 1 to: 942
KIEE0439.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 167 593 942
Size: 167 426 349
Fragments arranged by size:
426 349 167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 942
Size: 391 551
AleI CACnn'nnGTG
Cuts at: 0 157 942
Size: 157 785
AlwNI CAG_nnn'CTG
Cuts at: 0 145 526 942
Size: 145 381 416
Fragments arranged by size:
416 381 145
ApaI G_GGCC'C
Cuts at: 0 772 942
Size: 772 170
ApoI r'AATT_y
Cuts at: 0 271 942
Size: 271 671
AvrII C'CTAG_G
Cuts at: 0 609 942
Size: 609 333
BanI G'GyrC_C
Cuts at: 0 115 942
Size: 115 827
BanII G_rGCy'C
Cuts at: 0 389 772 942
Size: 389 383 170
Fragments arranged by size:
389 383 170
BbeI G_GCGC'C
Cuts at: 0 119 942
Size: 119 823
BbsI GAAGACnn'nnnn_
Cuts at: 0 289 580 731 919 942
Size: 289 291 151 188 23
Fragments arranged by size:
291 289 188 151 23
BciVI GTATCCnnnnn_n'
Cuts at: 0 795 942
Size: 795 147
Bme1580I G_kGCm'C
Cuts at: 0 772 942
Size: 772 170
BmrI ACTGGGnnnn_n'
Cuts at: 0 514 942
Size: 514 428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 938 942
Size: 938 4
Bpu10I CC'TnA_GC
Cuts at: 0 289 942
Size: 289 653
BsaAI yAC'GTr
Cuts at: 0 87 942
Size: 87 855
BsaHI Gr'CG_yC
Cuts at: 0 116 942
Size: 116 826
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 942
Size: 430 30 482
Fragments arranged by size:
482 430 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 30 942
Size: 30 912
BseYI C'CCAG_C
Cuts at: 0 865 942
Size: 865 77
BsiHKAI G_wGCw'C
Cuts at: 0 295 389 942
Size: 295 94 553
Fragments arranged by size:
553 295 94
Bsp1286I G_dGCh'C
Cuts at: 0 295 389 772 942
Size: 295 94 383 170
Fragments arranged by size:
383 295 170 94
BspHI T'CATG_A
Cuts at: 0 530 942
Size: 530 412
BsrDI GCAATG_nn'
Cuts at: 0 122 942
Size: 122 820
BsrFI r'CCGG_y
Cuts at: 0 118 262 754 942
Size: 118 144 492 188
Fragments arranged by size:
492 188 144 118
BstAPI GCAn_nnn'nTGC
Cuts at: 0 24 942
Size: 24 918
BstEII G'GTnAC_C
Cuts at: 0 757 942
Size: 757 185
Bsu36I CC'TnA_GG
Cuts at: 0 220 257 942
Size: 220 37 685
Fragments arranged by size:
685 220 37
BtgI C'CryG_G
Cuts at: 0 764 942
Size: 764 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 281 942
Size: 281 661
BtsI GCAGTG_nn'
Cuts at: 0 734 942
Size: 734 208
EaeI y'GGCC_r
Cuts at: 0 62 942
Size: 62 880
EarI CTCTTCn'nnn_
Cuts at: 0 394 942
Size: 394 548
EcoICRI GAG'CTC
Cuts at: 0 387 942
Size: 387 555
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 938 942
Size: 391 547 4
Fragments arranged by size:
547 391 4
HaeII r_GCGC'y
Cuts at: 0 119 942
Size: 119 823
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 942
Size: 304 32 606
Fragments arranged by size:
606 304 32
KasI G'GCGC_C
Cuts at: 0 115 942
Size: 115 827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 942
Size: 774 168
MslI CAynn'nnrTG
Cuts at: 0 157 739 942
Size: 157 582 203
Fragments arranged by size:
582 203 157
NaeI GCC'GGC
Cuts at: 0 120 264 942
Size: 120 144 678
Fragments arranged by size:
678 144 120
NarI GG'CG_CC
Cuts at: 0 116 942
Size: 116 826
NcoI C'CATG_G
Cuts at: 0 764 942
Size: 764 178
NgoMIV G'CCGG_C
Cuts at: 0 118 262 942
Size: 118 144 680
Fragments arranged by size:
680 144 118
PmlI CAC'GTG
Cuts at: 0 87 942
Size: 87 855
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 886 918 942
Size: 886 32 24
Fragments arranged by size:
886 32 24
PspOMI G'GGCC_C
Cuts at: 0 768 942
Size: 768 174
PstI C_TGCA'G
Cuts at: 0 141 942
Size: 141 801
SacI G_AGCT'C
Cuts at: 0 389 942
Size: 389 553
SapI GCTCTTCn'nnn_
Cuts at: 0 394 942
Size: 394 548
SfcI C'TryA_G
Cuts at: 0 137 942
Size: 137 805
SfoI GGC'GCC
Cuts at: 0 117 942
Size: 117 825
StyI C'CwwG_G
Cuts at: 0 609 764 942
Size: 609 155 178
Fragments arranged by size:
609 178 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 506 815 942
Size: 506 309 127
Fragments arranged by size:
506 309 127
XbaI T'CTAG_A
Cuts at: 0 783 942
Size: 783 159
XmnI GAAnn'nnTTC
Cuts at: 0 901 942
Size: 901 41
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaI ApoI AvrII BanI
BanII BbeI BbsI BciVI Bme1580I BmrI BpmI Bpu10I
BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspHI
BsrDI BsrFI BstAPI BstEII Bsu36I BtgI BtgZI BtsI
EaeI EarI EcoICRI Eco57MI HaeII Hin4I KasI MmeI
MslI NaeI NarI NcoI NgoMIV PmlI PpiI PspOMI
PstI SacI SapI SfcI SfoI StyI TaqII XbaI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvaI
BaeI BamHI BbvCI BcgI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI BsaWI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I ClaI
CspCI DraI DraIII DrdI EagI EciI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MscI MspA1I NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XcmI
XhoI XmaI ZraI