################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AccII AclI AcsI AflII AhdI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BciVI BclI BfrI BglII BlnI BsaBI BsiWI Bsp1407I BspDI BspHI BsrGI BssHII Bst1107I BstUI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV FbaI FspI HindIII HpaI KasI KspI MamI MfeI MluI MseI MunI MvnI NarI NdeI NotI NruI PacI PaeR7I PflFI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfoI SgrAI SpeI SphI SspBI SspI SwaI Tru9I Tth111I XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 2754 GGTACC G^GTACC AccI 1466 GTAGAC GT^[AC][GT]AC AccIII 1185 TCCGGA T^CCGGA AcyI 1292 GACGCC G[GA]^CG[CT]C AflIII 1728 ACATGT A^C[GA][CT]GT AgeI 2505 ACCGGT A^CCGGT Aor51HI 1418 AGCGCT AGC^GCT Asp700I 2917 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2754 GGTACC G^GTACC BbrPI 685 CACGTG CAC^GTG BbsI 1181 GTCTTC GAAGAC(2/6) BpuAI 1181 GTCTTC GAAGAC(2/6) BsaHI 1292 GACGCC G[GA]^CG[CT]C BseAI 1185 TCCGGA T^CCGGA BsiEI 2864 CGGCCG CG[GA][CT]^CG BsmBI 1290 GAGACG CGTCTC(1/5) BsmI 569 GCATTC GAATGC(1/-1) BspEI 1185 TCCGGA T^CCGGA BspLU11I 1728 ACATGT A^CATGT BsrBI 1531 CCGCTC CCGCTC(-3/-3) BsrDI 2556 CATTGC GCAATG(2/0) BssSI 460 CTCGTG CACGAG(-5/-1) BstBI 18 TTCGAA TT^CGAA BstEII 2873 GGTCACC G^GT[ACGT]ACC BstPI 2873 GGTCACC G^GT[ACGT]ACC DraIII 408 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2778 GACCCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2864 CGGCCG C^GGCCG EclXI 2864 CGGCCG C^GGCCG Eco47III 1418 AGCGCT AGC^GCT Eco52I 2864 CGGCCG C^GGCCG EcoNI 867 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2873 GGTCACC G^GT[ACGT]ACC EcoT22I 2212 ATGCAT ATGCA^T FseI 913 GGCCGGCC GGCCGG^CC Hin1I 1292 GACGCC G[GA]^CG[CT]C KpnI 2754 GGTACC GGTAC^C MroI 1185 TCCGGA T^CCGGA NaeI 914 GCCGGC GCC^GGC NcoI 909 CCATGG C^CATGG NgoMIV 914 GCCGGC G^CCGGC NheI 2473 GCTAGC G^CTAGC NsiI 2212 ATGCAT ATGCA^T NspI 1728 ACATGT [GA]CATG^[CT] NspV 18 TTCGAA TT^CGAA PinAI 2505 ACCGGT A^CCGGT PleI 2785 GAGTC GAGTC(4/5) PmaCI 685 CACGTG CAC^GTG PmlI 685 CACGTG CAC^GTG PshAI 788 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 117 CCTGCAGG CCTGCA^GG SfuI 18 TTCGAA TT^CGAA SnaBI 2179 TACGTA TAC^GTA Sse8387I 117 CCTGCAGG CCTGCA^GG XbaI 2316 TCTAGA T^CTAGA XmnI 2917 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Alw44I 316 GTGCAC G^TGCAC 884 GTGCAC AocI 1213 CCTGAGG CC^T[ACGT]AGG 2904 CCTCAGG ApaI 1841 GGGCCC GGGCC^C 2397 GGGCCC ApaLI 316 GTGCAC G^TGCAC 884 GTGCAC BbvCI 1757 CCTCAGC CCTCAGC(-5/-2) 2064 GCTGAGG BsaAI 685 CACGTG [CT]AC^GT[GA] 2179 TACGTA BsaI 771 GAGACC GGTCTC(1/5) 1882 GAGACC BsaWI 1185 TCCGGA [AT]^CCGG[AT] 2505 ACCGGT BsrFI 914 GCCGGC [GA]^CCGG[CT] 2505 ACCGGT Bsu36I 1213 CCTGAGG CC^T[ACGT]AGG 2904 CCTCAGG Cfr10I 914 GCCGGC [GA]^CCGG[CT] 2505 ACCGGT DsaI 909 CCATGG C^C[GA][CT]GG 2623 CCACGG Eco81I 1213 CCTGAGG CC^T[ACGT]AGG 2904 CCTCAGG HincII 1399 GTTGAC GT[CT]^[GA]AC 2509 GTCAAC HindII 1399 GTTGAC GT[CT]^[GA]AC 2509 GTCAAC SapI 1000 GAAGAGC GCTCTTC(1/4) 1012 GAAGAGC TaqI 19 TCGA T^CGA 1175 TCGA TfiI 87 GATTC G^A[AT]TC 2499 GATTC TthHB8I 19 TCGA T^CGA 1175 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BamHI BanI BanII BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI SmlI StuI StyI TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmaCI XmaI