Restriction map for pFN21AE3655
 (Circular) (Six-base) MAPSORT of: pFN21AE3655.seq  Check: 3459  from: 1  to: 7741



pFN21AE3655  7741 bp

 With 182 enzymes: SgfI * 

                        December 28, 2011 15:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2086    2213    3056    4911    4960    2086 
   Size:        127     843    1855      49    4867

  Fragments arranged by size: 

                4867    1855     843     127      49
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    5551     278 
   Size:         53      83     186     936    4015    2468

  Fragments arranged by size: 

                4015    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3472    5084    1937 
   Size:       1535    1612    4594

  Fragments arranged by size: 

                4594    1612    1535
 
Acc65I G'GTAC_C

Cuts at:   4759    5062    4759 
   Size:        303    7438
 
AclI AA'CG_TT

Cuts at:   1563    5868    6241    1563 
   Size:       4305     373    3063

  Fragments arranged by size: 

                4305    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2423    2787    3011    3143    3436    3692    3980    4286 
   Size:        791     364     224     132     293     256     288     306

Cuts at:   4286    4433    4751    4775    4793    5804    7188    7456    1632 
   Size:        147     318      24      18    1011    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1011     791     364     318     306     293
                 288     268     256     224     147     132      24      18
            
 
AfeI AGC'GCT

Cuts at:   3425    3425 
   Size:       7741
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    7544
 
AflIII A'CryG_T

Cuts at:   1203    3733    6640    7617    1203 
   Size:       2530    2907     977    1327

  Fragments arranged by size: 

                2907    2530    1327     977
 
AgeI A'CCGG_T

Cuts at:   4510    6572    4510 
   Size:       2062    5679
 
AhdI GACnn_n'nnGTC

Cuts at:   6470    6470 
   Size:       7741
 
AleI CACnn'nnGTG

Cuts at:   1166    2328    1166 
   Size:       1162    6579
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       7741
 
AlwNI CAG_nnn'CTG

Cuts at:   2131    2957    3107    3500    3776    4069    4580    4903    4936 
   Size:        826     150     393     276     293     511     323      33

Cuts at:   4936    5484    7056    2131 
   Size:        548    1572    2816

  Fragments arranged by size: 

                2816    1572     826     548     511     393     323     293
                 276     150      33
 
ApaI G_GGCC'C

Cuts at:   3850    4406    3850 
   Size:        556    7185
 
ApaLI G'TGCA_C

Cuts at:   2321    2889    5798    6954    2321 
   Size:        568    2909    1156    3108

  Fragments arranged by size: 

                3108    2909    1156     568
 
ApoI r'AATT_y

Cuts at:   1493    5050    5276    5428    7537    1493 
   Size:       3557     226     152    2109    1697

  Fragments arranged by size: 

                3557    2109    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    6295     160 
   Size:        872    5263    1606

  Fragments arranged by size: 

                5263    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7741
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2457    2638    2973    3504    5066    1870 
   Size:         73     514     181     335     531    1562    4545

  Fragments arranged by size: 

                4545    1562     531     514     335     181      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2734    2767    2734 
   Size:         33    7708
 
BamHI G'GATC_C

Cuts at:   2938    3553    4836    5071    5438    2938 
   Size:        615    1283     235     367    5241

  Fragments arranged by size: 

                5241    1283     615     367     235
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2411    3326    3701    4759    4948 
   Size:        325     217     627     624     915     375    1058     189

Cuts at:   4948    5062    6517     618 
   Size:        114    1455    1842

  Fragments arranged by size: 

                1842    1455    1058     915     627     624     375     325
                 217     189     114
 
BanII G_rGCy'C

Cuts at:   1391    2075    2286    2567    2664    2846    3080    3207    3757 
   Size:        684     211     281      97     182     234     127     550

Cuts at:   3757    3850    4024    4406    5060    1391 
   Size:         93     174     382     654    4072

  Fragments arranged by size: 

                4072     684     654     550     382     281     234     211
                 182     174     127      97      93
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    3179     928 
   Size:        971    1280    5490

  Fragments arranged by size: 

                5490    1280     971
 
BbvCI CC'TCA_GC

Cuts at:   3763    4070    3763 
   Size:        307    7434
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2204    2238    3678    3712    4994    5028    5932 
   Size:         34     267      34    1440      34    1282      34     904

Cuts at:   5932    5966    1903 
   Size:         34    3678

  Fragments arranged by size: 

                3678    1440    1282     904     267      34      34      34
                  34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   5633    6843    5633 
   Size:       1210    6531
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       7741
 
BfrBI ATG'CAT

Cuts at:   4219    4219 
   Size:       7741
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2127    2144    4596 
   Size:        107     122      71     631     605     455      17    2452

Cuts at:   4596    6352     136 
   Size:       1756    1525

  Fragments arranged by size: 

                2452    1756    1525     631     605     455     122     107
                  71      17
 
BglII A'GATC_T

Cuts at:   7736    7736 
   Size:       7741
 
BlpI GC'TnA_GC

Cuts at:   2292    4852    4985    5158    2292 
   Size:       2560     133     173    4875

  Fragments arranged by size: 

                4875    2560     173     133
 
Bme1580I G_kGCm'C

Cuts at:   1790    2325    2414    2893    3232    3704    3850    4406    4525 
   Size:        535      89     479     339     472     146     556     119

Cuts at:   4525    5802    6958    1790 
   Size:       1277    1156    2573

  Fragments arranged by size: 

                2573    1277    1156     556     535     479     472     339
                 146     119      89
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       7741
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2221    3207    3380 
   Size:        416      53      83     399      24     804     986     173

Cuts at:   3380    5472    6430     442 
   Size:       2092     958    1753

  Fragments arranged by size: 

                2092    1753     986     958     804     416     399     173
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    4482    1056 
   Size:       3426    4315
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2411    2732    2804    3118    3704    4414    4622    4808 
   Size:       1172     321      72     314     586     710     208     186

Cuts at:   4808    6401    1239 
   Size:       1593    2579

  Fragments arranged by size: 

                2579    1593    1172     710     586     321     314     208
                 186      72
 
Bpu10I CC'TnA_GC

Cuts at:   3056    3763    4070    4356    3056 
   Size:        707     307     286    6441

  Fragments arranged by size: 

                6441     707     307     286
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2262    3094    4118    5866    6731    7029    2262 
   Size:        832    1024    1748     865     298    2974

  Fragments arranged by size: 

                2974    1748    1024     865     832     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2770    3881    6404     882 
   Size:        617    1271    1111    2523    2219

  Fragments arranged by size: 

                2523    2219    1271    1111     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2692    4186    5532     493 
   Size:        711    1488    1494    1346    2702

  Fragments arranged by size: 

                2702    1494    1488    1346     711
 
BsaBI GATnn'nnATC

Cuts at:   7735    7735 
   Size:       7741
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3298    5548    5930    6565 
   Size:         53      83     186     936    1765    2250     382     635

Cuts at:   6565     275 
   Size:       1451

  Fragments arranged by size: 

                2250    1765    1451     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   3190    4510    6174    6572    6846    6993    7404    3190 
   Size:       1320    1664     398     274     147     411    3527

  Fragments arranged by size: 

                3527    1664    1320     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2091    2121    2613    2643    2690 
   Size:         30     165      30     688      30     492      30      47

Cuts at:   2690    2720    3575    3605    1178 
   Size:         30     855      30    5314

  Fragments arranged by size: 

                5314     855     688     492     165      47      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2054    2225    2408    4169    1186 
   Size:        868     171     183    1761    4758

  Fragments arranged by size: 

                4758    1761     868     183     171
 
BseYI C'CCAG_C

Cuts at:   2068    2928    3094    4140    4555    4705    4766    4896    6944 
   Size:        860     166    1046     415     150      61     130    2048

Cuts at:   6944    7440    2068 
   Size:        496    2369

  Fragments arranged by size: 

                2369    2048    1046     860     496     415     166     150
                 130      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2528    2879    3084    3120    4233    4829    5012 
   Size:        573     792     351     205      36    1113     596     183

Cuts at:   5012    1163 
   Size:       3892

  Fragments arranged by size: 

                3892    1113     792     596     573     351     205     183
                  36
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4872    5191    5952    6980    1540 
   Size:        275     186    2871     319     761    1028    2301

  Fragments arranged by size: 

                2871    2301    1028     761     319     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2286    2325    2664    2846    2893    3207    3570    3834    5802 
   Size:         39     339     182      47     314     363     264    1968

Cuts at:   5802    5887    6958    2286 
   Size:         85    1071    3069

  Fragments arranged by size: 

                3069    1968    1071     363     339     314     264     182
                  85      47      39
 
BsmI GAATG_Cn'

Cuts at:   2574    5252    5345    2574 
   Size:       2678      93    4970

  Fragments arranged by size: 

                4970    2678      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3289    3289 
   Size:       7741
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2075    2286    2325    2414    2567    2664    2846 
   Size:        399     285     211      39      89     153      97     182

Cuts at:   2846    2893    3080    3207    3232    3570    3704    3757    3834 
   Size:         47     187     127      25     338     134      53      77

Cuts at:   3834    3850    4024    4406    4525    5060    5802    5887    6958 
   Size:         16     174     382     119     535     742      85    1071

Cuts at:   6958    1391 
   Size:       2174

  Fragments arranged by size: 

                2174    1071     742     535     399     382     338     285
                 211     187     182     174     153     134     127     119
                  97      89      85      77      53      47      39      25
                  16
 
BspEI T'CCGG_A

Cuts at:   3190    7404    3190 
   Size:       4214    3527
 
BspHI T'CATG_A

Cuts at:   5630    7360    5630 
   Size:       1730    6011
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2086    2213    2355    3056    4911    4960    5096     844 
   Size:       1242     127     142     701    1855      49     136    3489

  Fragments arranged by size: 

                3489    1855    1242     701     142     136     127      49
            
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3538    5628    1394 
   Size:        429    1715    2090    3507

  Fragments arranged by size: 

                3507    2090    1715     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4560    6236    6410      65 
   Size:       1183    3312    1676     174    1396

  Fragments arranged by size: 

                3312    1676    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2919    4510    6385    6572    1700 
   Size:        110    1109    1591    1875     187    2869

  Fragments arranged by size: 

                2869    1875    1591    1109     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       7741
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       7741
 
BssSI C'ACGA_G

Cuts at:   2465    5801    6813    2465 
   Size:       3336    1012    3393

  Fragments arranged by size: 

                3393    3336    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3107    3500    3838    4225     794 
   Size:       2313     393     338     387    4310

  Fragments arranged by size: 

                4310    2313     393     387     338
 
BstBI TT'CG_AA

Cuts at:   2024    6545    2024 
   Size:       4521    3220
 
BstEII G'GTnAC_C

Cuts at:   1184    4878    1184 
   Size:       3694    4047
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2110    2155    3467    3999    4220    2110 
   Size:         45    1312     532     221    5631

  Fragments arranged by size: 

                5631    1312     532     221      45
 
BstYI r'GATC_y

Cuts at:   1972    2938    3553    4836    5071    5438    5823    5840    7292 
   Size:        966     615    1283     235     367     385      17    1452

Cuts at:   7292    7378    7390    7736    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1283     966     615     385     367     346
                 235      86      17      12
 
Bsu36I CC'TnA_GG

Cuts at:   3219    4910    3219 
   Size:       1691    6050
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2914    4628     513 
   Size:        552     939     910    1714    3626

  Fragments arranged by size: 

                3626    1714     939     910     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    7002

  Fragments arranged by size: 

                7002     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2866    2891    3067    3270    3677    3718    3848    5010    5257 
   Size:         25     176     203     407      41     130    1162     247

Cuts at:   5257    6051    6071    2866 
   Size:        794      20    4536

  Fragments arranged by size: 

                4536    1162     794     407     247     203     176     130
                  41      25      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       7741
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    7706
 
DraI TTT'AAA

Cuts at:   5045    5045 
   Size:       7741
 
DraIII CAC_nnn'GTG

Cuts at:   2418    2418 
   Size:       7741
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4789    6748     809 
   Size:        507    3473    1959    1802

  Fragments arranged by size: 

                3473    1959    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2145    2917    2921 
   Size:         54    1425      50     275      18     315     772       4

Cuts at:   2921    3087    4027    4869    5188    6077    7706       8 
   Size:        166     940     842     319     889    1629      43

  Fragments arranged by size: 

                1629    1425     940     889     842     772     319     315
                 275     166      54      50      43      18       4
 
EagI C'GGCC_G

Cuts at:   1537    1812    4869    5188    1537 
   Size:        275    3057     319    4090

  Fragments arranged by size: 

                4090    3057     319     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3000    3012    3455    3875    5671    1353 
   Size:       1647      12     443     420    1796    3423

  Fragments arranged by size: 

                3423    1796    1647     443     420      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    6328    6702    6848     387 
   Size:       1064     138    4739     374     146    1280

  Fragments arranged by size: 

                4739    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2284    2662    2844    3205    2284 
   Size:        378     182     361    6820

  Fragments arranged by size: 

                6820     378     361     182
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2411    2423    2732    2787    2804    3011    3118 
   Size:        393     779      12     309      55      17     207     107

Cuts at:   3118    3143    3436    3692    3704    3980    4286    4414    4433 
   Size:         25     293     256      12     276     306     128      19

Cuts at:   4433    4622    4751    4775    4793    4808    5804    6401    7188 
   Size:        189     129      24      18      15     996     597     787

Cuts at:   7188    7456    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     996     787     779     597     393     309     306
                 293     276     268     256     207     189     129     128
                 107      55      25      24      19      18      17      15
                  12      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2876    2876 
   Size:       7741
 
EcoO109I rG'GnC_Cy

Cuts at:   2107    2128    2344    2461    2512    2706    2977    3034    3456 
   Size:         21     216     117      51     194     271      57     422

Cuts at:   3456    3508    3976    4124    4403    4782    2107 
   Size:         52     468     148     279     379    5066

  Fragments arranged by size: 

                5066     468     422     379     279     271     216     194
                 148     117      57      52      51      21
 
EcoRI G'AATT_C

Cuts at:   5050    5428    5050 
   Size:        378    7363
 
FseI GG_CCGG'CC

Cuts at:   2923    2923 
   Size:       7741
 
FspI TGC'GCA

Cuts at:   6247    6247 
   Size:       7741
 
HaeII r_GCGC'y

Cuts at:   1678    3324    3427    3643    6888    1678 
   Size:       1646     103     216    3245    2531

  Fragments arranged by size: 

                3245    2531    1646     216     103
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2091    2123    2666    2690    2698 
   Size:         32     135      32     519      32     543      24       8

Cuts at:   2698    2722    3973    4005    4708    4740    6382    6414    6456 
   Size:         24    1251      32     703      32    1642      32      42

Cuts at:   6456    6488    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1642    1251     703     543     519     135      42
                  32      32      32      32      32      32      32      24
                  24       8
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3406    4516    5085    5331     669 
   Size:        988     281    1468    1110     569     246    3079

  Fragments arranged by size: 

                3079    1468    1110     988     569     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       7741
 
HpaI GTT'AAC

Cuts at:   5331    5331 
   Size:       7741
 
KpnI G_GTAC'C

Cuts at:   4763    5066    4763 
   Size:        303    7438
 
MfeI C'AATT_G

Cuts at:   5340    5340 
   Size:       7741
 
MluI A'CGCG_T

Cuts at:   7617    7617 
   Size:       7741
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2238    2334    3898    6855    7039    1305 
   Size:        933      96    1564    2957     184    2007

  Fragments arranged by size: 

                2957    2007    1564     933     184      96
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2147    3089    4029      10 
   Size:         54    1425     343     315     942     940    3722

  Fragments arranged by size: 

                3722    1425     942     940     343     315      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2328    2427    2913    3573    5699    6058    6217 
   Size:        648    1162      99     486     660    2126     359     159

Cuts at:   6217     518 
   Size:       2042

  Fragments arranged by size: 

                2126    2042    1162     660     648     486     359     159
                  99
 
MspA1I CmG'CkG

Cuts at:   1606    2059    2401    2540    3387    3500    3767    4066    4196 
   Size:        453     342     139     847     113     267     299     130

Cuts at:   4196    4580    4766    4936    4966    5157    5834    6982    7227 
   Size:        384     186     170      30     191     677    1148     245

Cuts at:   7227    1606 
   Size:       2120

  Fragments arranged by size: 

                2120    1148     847     677     453     384     342     299
                 267     245     191     186     170     139     130     113
                  30
 
NaeI GCC'GGC

Cuts at:   2921    2921 
   Size:       7741
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2914     513 
   Size:        552     939     910    5340

  Fragments arranged by size: 

                5340     939     910     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       7741
 
NgoMIV G'CCGG_C

Cuts at:   2919    2919 
   Size:       7741
 
NheI G'CTAG_C

Cuts at:   1052    4478    1052 
   Size:       3426    4315
 
NotI GC'GGCC_GC

Cuts at:   5188    5188 
   Size:       7741
 
NsiI A_TGCA'T

Cuts at:   4221    4221 
   Size:       7741
 
NspI r_CATG'y

Cuts at:   1132    1229    3737    5099    6644    1132 
   Size:         97    2508    1362    1545    2229

  Fragments arranged by size: 

                2508    2229    1545    1362      97
 
PasI CC'CwG_GG

Cuts at:   1348    2560    2934    1348 
   Size:       1212     374    6155

  Fragments arranged by size: 

                6155    1212     374
 
PciI A'CATG_T

Cuts at:   3733    6640    3733 
   Size:       2907    4834
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2208    2368    2833    3971    4147    4297    1108 
   Size:       1100     160     465    1138     176     150    4552

  Fragments arranged by size: 

                4552    1138    1100     465     176     160     150
 
PfoI T'CCnGG_A

Cuts at:   3134    3134 
   Size:       7741
 
PmeI GTTT'AAAC

Cuts at:   5045    5045 
   Size:       7741
 
PmlI CAC'GTG

Cuts at:   2692    2692 
   Size:       7741
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5810    5842    7323    7355    5810 
   Size:         32    1481      32    6196

  Fragments arranged by size: 

                6196    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2107    2344    2512    2706    2977    3456    3976    4124    4782 
   Size:        237     168     194     271     479     520     148     658

Cuts at:   4782    2107 
   Size:       5066

  Fragments arranged by size: 

                5066     658     520     479     271     237     194     168
                 148
 
PshAI GACnn'nnGTC

Cuts at:   1613    2797    1613 
   Size:       1184    6557
 
PsiI TTA'TAA

Cuts at:   5311    5311 
   Size:       7741
 
PspOMI G'GGCC_C

Cuts at:   3846    4402    3846 
   Size:        556    7185
 
PstI C_TGCA'G

Cuts at:    830    2127    3496    3772    3843    3886    5093     830 
   Size:       1297    1369     276      71      43    1207    3478

  Fragments arranged by size: 

                3478    1369    1297    1207     276      71      43
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       7741
 
PvuII CAG'CTG

Cuts at:   2059    2401    2540    3500    3767    4066    4196    4580    4766 
   Size:        342     139     960     267     299     130     384     186

Cuts at:   4766    4936    2059 
   Size:        170    4864

  Fragments arranged by size: 

                4864     960     384     342     299     267     186     170
                 139     130
 
SacI G_AGCT'C

Cuts at:   2286    2664    2846    3207    2286 
   Size:        378     182     361    6820

  Fragments arranged by size: 

                6820     378     361     182
 
SalI G'TCGA_C

Cuts at:   1936    5083    1936 
   Size:       3147    4594
 
SanDI GG'GwC_CC

Cuts at:   2706    2706 
   Size:       7741
 
SapI GCTCTTCn'nnn_

Cuts at:   3000    3012    3000 
   Size:         12    7729
 
SbfI CC_TGCA'GG

Cuts at:   2127    5093    2127 
   Size:       2966    4775
 
ScaI AGT'ACT

Cuts at:   5989    7701    5989 
   Size:       1712    6029
 
SfcI C'TryA_G

Cuts at:    826    1045    2123    2958    3492    3717    3748    3768    3839 
   Size:        219    1078     835     534     225      31      20      71

Cuts at:   3839    3882    4572    5089    6224    6905    7096     826 
   Size:         43     690     517    1135     681     191    1471

  Fragments arranged by size: 

                1471    1135    1078     835     690     681     534     517
                 225     219     191      71      43      31      20
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   2127    2144    4596    2127 
   Size:         17    2452    5272

  Fragments arranged by size: 

                5272    2452      17
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       7741
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       7741
 
SmaI CCC'GGG

Cuts at:   1872    2459    2975    3506    5068    1872 
   Size:        587     516     531    1562    4545

  Fragments arranged by size: 

                4545    1562     587     531     516
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2277    3073    4097    5845    6746    7008 
   Size:        197     926     334     796    1024    1748     901     262

Cuts at:   7008     820 
   Size:       1553

  Fragments arranged by size: 

                1748    1553    1024     926     901     796     334     262
                 197
 
SnaBI TAC'GTA

Cuts at:    493    4186    5532     493 
   Size:       3693    1346    2702

  Fragments arranged by size: 

                3693    2702    1346
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       7741
 
SphI G_CATG'C

Cuts at:   1132    5099    1132 
   Size:       3967    3774
 
SspI AAT'ATT

Cuts at:      5      52    5665       5 
   Size:         47    5613    2081

  Fragments arranged by size: 

                5613    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2121    2273    2882    3746    4590    1517 
   Size:        604     152     609     864     844    4668

  Fragments arranged by size: 

                4668     864     844     609     604     152
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2914    3477    4146    5180     513 
   Size:        552     939     910     563     669    1034    3074

  Fragments arranged by size: 

                3074    1034     939     910     669     563     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2156    3086    3677    5768    5785    5938    6123 
   Size:       1255     576     930     591    2091      17     153     185

Cuts at:   6123    6568     325 
   Size:        445    1498

  Fragments arranged by size: 

                2091    1498    1255     930     591     576     445     185
                 153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    5987    7699      96 
   Size:        274      80      33      51    1443    4010    1712     138

  Fragments arranged by size: 

                4010    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2613    2645    7701    7733    2613 
   Size:         32    5056      32    2621

  Fragments arranged by size: 

                5056    2621      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       7741
 
XbaI T'CTAG_A

Cuts at:   4321    5077    4321 
   Size:        756    6985
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2011    2567    2912    2931    3484    2011 
   Size:        556     345      19     553    6268

  Fragments arranged by size: 

                6268     556     553     345      19
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       7741
 
XmaI C'CCGG_G

Cuts at:   1870    2457    2973    3504    5066    1870 
   Size:        587     516     531    1562    4545

  Fragments arranged by size: 

                4545    1562     587     531     516
 
XmnI GAAnn'nnTTC

Cuts at:   4926    5870    4926 
   Size:        944    6797
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    5549     276 
   Size:         53      83     186     936    4015    2468

  Fragments arranged by size: 

                4015    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FseI     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI
     PvuI    PvuII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     AscI    AvrII     BbeI     BplI    BsiWI  BstZ17I    EcoRV
     FalI    FspAI     KasI     NarI     NruI     PacI    PspXI     PsrI
    RsrII    SacII    SexAI     SfoI     SrfI     SwaI
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