(Circular) (Six-base) MAPSORT of: pFN21AE3655.seq Check: 3459 from: 1 to: 7741 pFN21AE3655 7741 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2086 2213 3056 4911 4960 2086 Size: 127 843 1855 49 4867 Fragments arranged by size: 4867 1855 843 127 49 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5551 278 Size: 53 83 186 936 4015 2468 Fragments arranged by size: 4015 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3472 5084 1937 Size: 1535 1612 4594 Fragments arranged by size: 4594 1612 1535 Acc65I G'GTAC_C Cuts at: 4759 5062 4759 Size: 303 7438 AclI AA'CG_TT Cuts at: 1563 5868 6241 1563 Size: 4305 373 3063 Fragments arranged by size: 4305 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2423 2787 3011 3143 3436 3692 3980 4286 Size: 791 364 224 132 293 256 288 306 Cuts at: 4286 4433 4751 4775 4793 5804 7188 7456 1632 Size: 147 318 24 18 1011 1384 268 1917 Fragments arranged by size: 1917 1384 1011 791 364 318 306 293 288 268 256 224 147 132 24 18 AfeI AGC'GCT Cuts at: 3425 3425 Size: 7741 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7544 AflIII A'CryG_T Cuts at: 1203 3733 6640 7617 1203 Size: 2530 2907 977 1327 Fragments arranged by size: 2907 2530 1327 977 AgeI A'CCGG_T Cuts at: 4510 6572 4510 Size: 2062 5679 AhdI GACnn_n'nnGTC Cuts at: 6470 6470 Size: 7741 AleI CACnn'nnGTG Cuts at: 1166 2328 1166 Size: 1162 6579 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7741 AlwNI CAG_nnn'CTG Cuts at: 2131 2957 3107 3500 3776 4069 4580 4903 4936 Size: 826 150 393 276 293 511 323 33 Cuts at: 4936 5484 7056 2131 Size: 548 1572 2816 Fragments arranged by size: 2816 1572 826 548 511 393 323 293 276 150 33 ApaI G_GGCC'C Cuts at: 3850 4406 3850 Size: 556 7185 ApaLI G'TGCA_C Cuts at: 2321 2889 5798 6954 2321 Size: 568 2909 1156 3108 Fragments arranged by size: 3108 2909 1156 568 ApoI r'AATT_y Cuts at: 1493 5050 5276 5428 7537 1493 Size: 3557 226 152 2109 1697 Fragments arranged by size: 3557 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6295 160 Size: 872 5263 1606 Fragments arranged by size: 5263 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7741 AvaI C'yCGr_G Cuts at: 1870 1943 2457 2638 2973 3504 5066 1870 Size: 73 514 181 335 531 1562 4545 Fragments arranged by size: 4545 1562 531 514 335 181 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2734 2767 2734 Size: 33 7708 BamHI G'GATC_C Cuts at: 2938 3553 4836 5071 5438 2938 Size: 615 1283 235 367 5241 Fragments arranged by size: 5241 1283 615 367 235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2411 3326 3701 4759 4948 Size: 325 217 627 624 915 375 1058 189 Cuts at: 4948 5062 6517 618 Size: 114 1455 1842 Fragments arranged by size: 1842 1455 1058 915 627 624 375 325 217 189 114 BanII G_rGCy'C Cuts at: 1391 2075 2286 2567 2664 2846 3080 3207 3757 Size: 684 211 281 97 182 234 127 550 Cuts at: 3757 3850 4024 4406 5060 1391 Size: 93 174 382 654 4072 Fragments arranged by size: 4072 684 654 550 382 281 234 211 182 174 127 97 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3179 928 Size: 971 1280 5490 Fragments arranged by size: 5490 1280 971 BbvCI CC'TCA_GC Cuts at: 3763 4070 3763 Size: 307 7434 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2204 2238 3678 3712 4994 5028 5932 Size: 34 267 34 1440 34 1282 34 904 Cuts at: 5932 5966 1903 Size: 34 3678 Fragments arranged by size: 3678 1440 1282 904 267 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5633 6843 5633 Size: 1210 6531 BclI T'GATC_A Cuts at: 1548 1548 Size: 7741 BfrBI ATG'CAT Cuts at: 4219 4219 Size: 7741 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2127 2144 4596 Size: 107 122 71 631 605 455 17 2452 Cuts at: 4596 6352 136 Size: 1756 1525 Fragments arranged by size: 2452 1756 1525 631 605 455 122 107 71 17 BglII A'GATC_T Cuts at: 7736 7736 Size: 7741 BlpI GC'TnA_GC Cuts at: 2292 4852 4985 5158 2292 Size: 2560 133 173 4875 Fragments arranged by size: 4875 2560 173 133 Bme1580I G_kGCm'C Cuts at: 1790 2325 2414 2893 3232 3704 3850 4406 4525 Size: 535 89 479 339 472 146 556 119 Cuts at: 4525 5802 6958 1790 Size: 1277 1156 2573 Fragments arranged by size: 2573 1277 1156 556 535 479 472 339 146 119 89 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7741 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2221 3207 3380 Size: 416 53 83 399 24 804 986 173 Cuts at: 3380 5472 6430 442 Size: 2092 958 1753 Fragments arranged by size: 2092 1753 986 958 804 416 399 173 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4482 1056 Size: 3426 4315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2411 2732 2804 3118 3704 4414 4622 4808 Size: 1172 321 72 314 586 710 208 186 Cuts at: 4808 6401 1239 Size: 1593 2579 Fragments arranged by size: 2579 1593 1172 710 586 321 314 208 186 72 Bpu10I CC'TnA_GC Cuts at: 3056 3763 4070 4356 3056 Size: 707 307 286 6441 Fragments arranged by size: 6441 707 307 286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2262 3094 4118 5866 6731 7029 2262 Size: 832 1024 1748 865 298 2974 Fragments arranged by size: 2974 1748 1024 865 832 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2770 3881 6404 882 Size: 617 1271 1111 2523 2219 Fragments arranged by size: 2523 2219 1271 1111 617 BsaAI yAC'GTr Cuts at: 493 1204 2692 4186 5532 493 Size: 711 1488 1494 1346 2702 Fragments arranged by size: 2702 1494 1488 1346 711 BsaBI GATnn'nnATC Cuts at: 7735 7735 Size: 7741 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3298 5548 5930 6565 Size: 53 83 186 936 1765 2250 382 635 Cuts at: 6565 275 Size: 1451 Fragments arranged by size: 2250 1765 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3190 4510 6174 6572 6846 6993 7404 3190 Size: 1320 1664 398 274 147 411 3527 Fragments arranged by size: 3527 1664 1320 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2091 2121 2613 2643 2690 Size: 30 165 30 688 30 492 30 47 Cuts at: 2690 2720 3575 3605 1178 Size: 30 855 30 5314 Fragments arranged by size: 5314 855 688 492 165 47 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2225 2408 4169 1186 Size: 868 171 183 1761 4758 Fragments arranged by size: 4758 1761 868 183 171 BseYI C'CCAG_C Cuts at: 2068 2928 3094 4140 4555 4705 4766 4896 6944 Size: 860 166 1046 415 150 61 130 2048 Cuts at: 6944 7440 2068 Size: 496 2369 Fragments arranged by size: 2369 2048 1046 860 496 415 166 150 130 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2528 2879 3084 3120 4233 4829 5012 Size: 573 792 351 205 36 1113 596 183 Cuts at: 5012 1163 Size: 3892 Fragments arranged by size: 3892 1113 792 596 573 351 205 183 36 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4872 5191 5952 6980 1540 Size: 275 186 2871 319 761 1028 2301 Fragments arranged by size: 2871 2301 1028 761 319 275 186 BsiHKAI G_wGCw'C Cuts at: 2286 2325 2664 2846 2893 3207 3570 3834 5802 Size: 39 339 182 47 314 363 264 1968 Cuts at: 5802 5887 6958 2286 Size: 85 1071 3069 Fragments arranged by size: 3069 1968 1071 363 339 314 264 182 85 47 39 BsmI GAATG_Cn' Cuts at: 2574 5252 5345 2574 Size: 2678 93 4970 Fragments arranged by size: 4970 2678 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3289 3289 Size: 7741 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2286 2325 2414 2567 2664 2846 Size: 399 285 211 39 89 153 97 182 Cuts at: 2846 2893 3080 3207 3232 3570 3704 3757 3834 Size: 47 187 127 25 338 134 53 77 Cuts at: 3834 3850 4024 4406 4525 5060 5802 5887 6958 Size: 16 174 382 119 535 742 85 1071 Cuts at: 6958 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 535 399 382 338 285 211 187 182 174 153 134 127 119 97 89 85 77 53 47 39 25 16 BspEI T'CCGG_A Cuts at: 3190 7404 3190 Size: 4214 3527 BspHI T'CATG_A Cuts at: 5630 7360 5630 Size: 1730 6011 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2086 2213 2355 3056 4911 4960 5096 844 Size: 1242 127 142 701 1855 49 136 3489 Fragments arranged by size: 3489 1855 1242 701 142 136 127 49 BsrBI CCG'CTC Cuts at: 1394 1823 3538 5628 1394 Size: 429 1715 2090 3507 Fragments arranged by size: 3507 2090 1715 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4560 6236 6410 65 Size: 1183 3312 1676 174 1396 Fragments arranged by size: 3312 1676 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2919 4510 6385 6572 1700 Size: 110 1109 1591 1875 187 2869 Fragments arranged by size: 2869 1875 1591 1109 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7741 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7741 BssSI C'ACGA_G Cuts at: 2465 5801 6813 2465 Size: 3336 1012 3393 Fragments arranged by size: 3393 3336 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3107 3500 3838 4225 794 Size: 2313 393 338 387 4310 Fragments arranged by size: 4310 2313 393 387 338 BstBI TT'CG_AA Cuts at: 2024 6545 2024 Size: 4521 3220 BstEII G'GTnAC_C Cuts at: 1184 4878 1184 Size: 3694 4047 BstXI CCAn_nnnn'nTGG Cuts at: 2110 2155 3467 3999 4220 2110 Size: 45 1312 532 221 5631 Fragments arranged by size: 5631 1312 532 221 45 BstYI r'GATC_y Cuts at: 1972 2938 3553 4836 5071 5438 5823 5840 7292 Size: 966 615 1283 235 367 385 17 1452 Cuts at: 7292 7378 7390 7736 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1283 966 615 385 367 346 235 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3219 4910 3219 Size: 1691 6050 BtgI C'CryG_G Cuts at: 513 1065 2004 2914 4628 513 Size: 552 939 910 1714 3626 Fragments arranged by size: 3626 1714 939 910 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7002 Fragments arranged by size: 7002 683 56 BtsI GCAGTG_nn' Cuts at: 2866 2891 3067 3270 3677 3718 3848 5010 5257 Size: 25 176 203 407 41 130 1162 247 Cuts at: 5257 6051 6071 2866 Size: 794 20 4536 Fragments arranged by size: 4536 1162 794 407 247 203 176 130 41 25 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7741 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7706 DraI TTT'AAA Cuts at: 5045 5045 Size: 7741 DraIII CAC_nnn'GTG Cuts at: 2418 2418 Size: 7741 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 6748 809 Size: 507 3473 1959 1802 Fragments arranged by size: 3473 1959 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2917 2921 Size: 54 1425 50 275 18 315 772 4 Cuts at: 2921 3087 4027 4869 5188 6077 7706 8 Size: 166 940 842 319 889 1629 43 Fragments arranged by size: 1629 1425 940 889 842 772 319 315 275 166 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 4869 5188 1537 Size: 275 3057 319 4090 Fragments arranged by size: 4090 3057 319 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3000 3012 3455 3875 5671 1353 Size: 1647 12 443 420 1796 3423 Fragments arranged by size: 3423 1796 1647 443 420 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6328 6702 6848 387 Size: 1064 138 4739 374 146 1280 Fragments arranged by size: 4739 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2284 2662 2844 3205 2284 Size: 378 182 361 6820 Fragments arranged by size: 6820 378 361 182 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2411 2423 2732 2787 2804 3011 3118 Size: 393 779 12 309 55 17 207 107 Cuts at: 3118 3143 3436 3692 3704 3980 4286 4414 4433 Size: 25 293 256 12 276 306 128 19 Cuts at: 4433 4622 4751 4775 4793 4808 5804 6401 7188 Size: 189 129 24 18 15 996 597 787 Cuts at: 7188 7456 1239 Size: 268 1524 Fragments arranged by size: 1524 996 787 779 597 393 309 306 293 276 268 256 207 189 129 128 107 55 25 24 19 18 17 15 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 2876 2876 Size: 7741 EcoO109I rG'GnC_Cy Cuts at: 2107 2128 2344 2461 2512 2706 2977 3034 3456 Size: 21 216 117 51 194 271 57 422 Cuts at: 3456 3508 3976 4124 4403 4782 2107 Size: 52 468 148 279 379 5066 Fragments arranged by size: 5066 468 422 379 279 271 216 194 148 117 57 52 51 21 EcoRI G'AATT_C Cuts at: 5050 5428 5050 Size: 378 7363 FseI GG_CCGG'CC Cuts at: 2923 2923 Size: 7741 FspI TGC'GCA Cuts at: 6247 6247 Size: 7741 HaeII r_GCGC'y Cuts at: 1678 3324 3427 3643 6888 1678 Size: 1646 103 216 3245 2531 Fragments arranged by size: 3245 2531 1646 216 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2123 2666 2690 2698 Size: 32 135 32 519 32 543 24 8 Cuts at: 2698 2722 3973 4005 4708 4740 6382 6414 6456 Size: 24 1251 32 703 32 1642 32 42 Cuts at: 6456 6488 1373 Size: 32 2626 Fragments arranged by size: 2626 1642 1251 703 543 519 135 42 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 3406 4516 5085 5331 669 Size: 988 281 1468 1110 569 246 3079 Fragments arranged by size: 3079 1468 1110 988 569 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7741 HpaI GTT'AAC Cuts at: 5331 5331 Size: 7741 KpnI G_GTAC'C Cuts at: 4763 5066 4763 Size: 303 7438 MfeI C'AATT_G Cuts at: 5340 5340 Size: 7741 MluI A'CGCG_T Cuts at: 7617 7617 Size: 7741 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2238 2334 3898 6855 7039 1305 Size: 933 96 1564 2957 184 2007 Fragments arranged by size: 2957 2007 1564 933 184 96 MscI TGG'CCA Cuts at: 10 64 1489 1832 2147 3089 4029 10 Size: 54 1425 343 315 942 940 3722 Fragments arranged by size: 3722 1425 942 940 343 315 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 2427 2913 3573 5699 6058 6217 Size: 648 1162 99 486 660 2126 359 159 Cuts at: 6217 518 Size: 2042 Fragments arranged by size: 2126 2042 1162 660 648 486 359 159 99 MspA1I CmG'CkG Cuts at: 1606 2059 2401 2540 3387 3500 3767 4066 4196 Size: 453 342 139 847 113 267 299 130 Cuts at: 4196 4580 4766 4936 4966 5157 5834 6982 7227 Size: 384 186 170 30 191 677 1148 245 Cuts at: 7227 1606 Size: 2120 Fragments arranged by size: 2120 1148 847 677 453 384 342 299 267 245 191 186 170 139 130 113 30 NaeI GCC'GGC Cuts at: 2921 2921 Size: 7741 NcoI C'CATG_G Cuts at: 513 1065 2004 2914 513 Size: 552 939 910 5340 Fragments arranged by size: 5340 939 910 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7741 NgoMIV G'CCGG_C Cuts at: 2919 2919 Size: 7741 NheI G'CTAG_C Cuts at: 1052 4478 1052 Size: 3426 4315 NotI GC'GGCC_GC Cuts at: 5188 5188 Size: 7741 NsiI A_TGCA'T Cuts at: 4221 4221 Size: 7741 NspI r_CATG'y Cuts at: 1132 1229 3737 5099 6644 1132 Size: 97 2508 1362 1545 2229 Fragments arranged by size: 2508 2229 1545 1362 97 PasI CC'CwG_GG Cuts at: 1348 2560 2934 1348 Size: 1212 374 6155 Fragments arranged by size: 6155 1212 374 PciI A'CATG_T Cuts at: 3733 6640 3733 Size: 2907 4834 PflMI CCAn_nnn'nTGG Cuts at: 1108 2208 2368 2833 3971 4147 4297 1108 Size: 1100 160 465 1138 176 150 4552 Fragments arranged by size: 4552 1138 1100 465 176 160 150 PfoI T'CCnGG_A Cuts at: 3134 3134 Size: 7741 PmeI GTTT'AAAC Cuts at: 5045 5045 Size: 7741 PmlI CAC'GTG Cuts at: 2692 2692 Size: 7741 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5810 5842 7323 7355 5810 Size: 32 1481 32 6196 Fragments arranged by size: 6196 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2107 2344 2512 2706 2977 3456 3976 4124 4782 Size: 237 168 194 271 479 520 148 658 Cuts at: 4782 2107 Size: 5066 Fragments arranged by size: 5066 658 520 479 271 237 194 168 148 PshAI GACnn'nnGTC Cuts at: 1613 2797 1613 Size: 1184 6557 PsiI TTA'TAA Cuts at: 5311 5311 Size: 7741 PspOMI G'GGCC_C Cuts at: 3846 4402 3846 Size: 556 7185 PstI C_TGCA'G Cuts at: 830 2127 3496 3772 3843 3886 5093 830 Size: 1297 1369 276 71 43 1207 3478 Fragments arranged by size: 3478 1369 1297 1207 276 71 43 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7741 PvuII CAG'CTG Cuts at: 2059 2401 2540 3500 3767 4066 4196 4580 4766 Size: 342 139 960 267 299 130 384 186 Cuts at: 4766 4936 2059 Size: 170 4864 Fragments arranged by size: 4864 960 384 342 299 267 186 170 139 130 SacI G_AGCT'C Cuts at: 2286 2664 2846 3207 2286 Size: 378 182 361 6820 Fragments arranged by size: 6820 378 361 182 SalI G'TCGA_C Cuts at: 1936 5083 1936 Size: 3147 4594 SanDI GG'GwC_CC Cuts at: 2706 2706 Size: 7741 SapI GCTCTTCn'nnn_ Cuts at: 3000 3012 3000 Size: 12 7729 SbfI CC_TGCA'GG Cuts at: 2127 5093 2127 Size: 2966 4775 ScaI AGT'ACT Cuts at: 5989 7701 5989 Size: 1712 6029 SfcI C'TryA_G Cuts at: 826 1045 2123 2958 3492 3717 3748 3768 3839 Size: 219 1078 835 534 225 31 20 71 Cuts at: 3839 3882 4572 5089 6224 6905 7096 826 Size: 43 690 517 1135 681 191 1471 Fragments arranged by size: 1471 1135 1078 835 690 681 534 517 225 219 191 71 43 31 20 SfiI GGCCn_nnn'nGGCC Cuts at: 2127 2144 4596 2127 Size: 17 2452 5272 Fragments arranged by size: 5272 2452 17 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7741 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7741 SmaI CCC'GGG Cuts at: 1872 2459 2975 3506 5068 1872 Size: 587 516 531 1562 4545 Fragments arranged by size: 4545 1562 587 531 516 SmlI C'TyrA_G Cuts at: 820 1017 1943 2277 3073 4097 5845 6746 7008 Size: 197 926 334 796 1024 1748 901 262 Cuts at: 7008 820 Size: 1553 Fragments arranged by size: 1748 1553 1024 926 901 796 334 262 197 SnaBI TAC'GTA Cuts at: 493 4186 5532 493 Size: 3693 1346 2702 Fragments arranged by size: 3693 2702 1346 SpeI A'CTAG_T Cuts at: 152 152 Size: 7741 SphI G_CATG'C Cuts at: 1132 5099 1132 Size: 3967 3774 SspI AAT'ATT Cuts at: 5 52 5665 5 Size: 47 5613 2081 Fragments arranged by size: 5613 2081 47 StuI AGG'CCT Cuts at: 1517 2121 2273 2882 3746 4590 1517 Size: 604 152 609 864 844 4668 Fragments arranged by size: 4668 864 844 609 604 152 StyI C'CwwG_G Cuts at: 513 1065 2004 2914 3477 4146 5180 513 Size: 552 939 910 563 669 1034 3074 Fragments arranged by size: 3074 1034 939 910 669 563 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2156 3086 3677 5768 5785 5938 6123 Size: 1255 576 930 591 2091 17 153 185 Cuts at: 6123 6568 325 Size: 445 1498 Fragments arranged by size: 2091 1498 1255 930 591 576 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5987 7699 96 Size: 274 80 33 51 1443 4010 1712 138 Fragments arranged by size: 4010 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2613 2645 7701 7733 2613 Size: 32 5056 32 2621 Fragments arranged by size: 5056 2621 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7741 XbaI T'CTAG_A Cuts at: 4321 5077 4321 Size: 756 6985 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2567 2912 2931 3484 2011 Size: 556 345 19 553 6268 Fragments arranged by size: 6268 556 553 345 19 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7741 XmaI C'CCGG_G Cuts at: 1870 2457 2973 3504 5066 1870 Size: 587 516 531 1562 4545 Fragments arranged by size: 4545 1562 587 531 516 XmnI GAAnn'nnTTC Cuts at: 4926 5870 4926 Size: 944 6797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5549 276 Size: 53 83 186 936 4015 2468 Fragments arranged by size: 4015 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BplI BsiWI BstZ17I EcoRV FalI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI