(Linear) (Six-base) MAPSORT of: KIEE3655.seq Check: 9066 from: 1 to: 3036 KIEE3655.orf With 182 enzymes: SgfI * December 27, 2011 16:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 81 208 1051 2906 2955 3036 Size: 81 127 843 1855 49 81 Fragments arranged by size: 1855 843 127 81 81 49 AccI GT'mk_AC Cuts at: 0 1467 3036 Size: 1467 1569 Acc65I G'GTAC_C Cuts at: 0 2754 3036 Size: 2754 282 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 782 1006 1138 1431 1687 1975 2281 Size: 418 364 224 132 293 256 288 306 Cuts at: 2281 2428 2746 2770 2788 3036 Size: 147 318 24 18 248 Fragments arranged by size: 418 364 318 306 293 288 256 248 224 147 132 24 18 AfeI AGC'GCT Cuts at: 0 1420 3036 Size: 1420 1616 AflIII A'CryG_T Cuts at: 0 1728 3036 Size: 1728 1308 AgeI A'CCGG_T Cuts at: 0 2505 3036 Size: 2505 531 AleI CACnn'nnGTG Cuts at: 0 323 3036 Size: 323 2713 AlwNI CAG_nnn'CTG Cuts at: 0 126 952 1102 1495 1771 2064 2575 2898 Size: 126 826 150 393 276 293 511 323 Cuts at: 2898 2931 3036 Size: 33 105 Fragments arranged by size: 826 511 393 323 293 276 150 126 105 33 ApaI G_GGCC'C Cuts at: 0 1845 2401 3036 Size: 1845 556 635 Fragments arranged by size: 1845 635 556 ApaLI G'TGCA_C Cuts at: 0 316 884 3036 Size: 316 568 2152 Fragments arranged by size: 2152 568 316 AvaI C'yCGr_G Cuts at: 0 452 633 968 1499 3036 Size: 452 181 335 531 1537 Fragments arranged by size: 1537 531 452 335 181 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 729 762 3036 Size: 729 33 2274 Fragments arranged by size: 2274 729 33 BamHI G'GATC_C Cuts at: 0 933 1548 2831 3036 Size: 933 615 1283 205 Fragments arranged by size: 1283 933 615 205 BanI G'GyrC_C Cuts at: 0 406 1321 1696 2754 2943 3036 Size: 406 915 375 1058 189 93 Fragments arranged by size: 1058 915 406 375 189 93 BanII G_rGCy'C Cuts at: 0 70 281 562 659 841 1075 1202 1752 Size: 70 211 281 97 182 234 127 550 Cuts at: 1752 1845 2019 2401 3036 Size: 93 174 382 635 Fragments arranged by size: 635 550 382 281 234 211 182 174 127 97 93 70 BbsI GAAGACnn'nnnn_ Cuts at: 0 1174 3036 Size: 1174 1862 BbvCI CC'TCA_GC Cuts at: 0 1758 2065 3036 Size: 1758 307 971 Fragments arranged by size: 1758 971 307 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 199 233 1673 1707 2989 3023 3036 Size: 199 34 1440 34 1282 34 13 Fragments arranged by size: 1440 1282 199 34 34 34 13 BfrBI ATG'CAT Cuts at: 0 2214 3036 Size: 2214 822 BglI GCCn_nnn'nGGC Cuts at: 0 122 139 2591 3036 Size: 122 17 2452 445 Fragments arranged by size: 2452 445 122 17 BlpI GC'TnA_GC Cuts at: 0 287 2847 2980 3036 Size: 287 2560 133 56 Fragments arranged by size: 2560 287 133 56 Bme1580I G_kGCm'C Cuts at: 0 320 409 888 1227 1699 1845 2401 2520 Size: 320 89 479 339 472 146 556 119 Cuts at: 2520 3036 Size: 516 Fragments arranged by size: 556 516 479 472 339 320 146 119 89 BmrI ACTGGGnnnn_n' Cuts at: 0 216 1202 1375 3036 Size: 216 986 173 1661 Fragments arranged by size: 1661 986 216 173 BmtI G_CTAG'C Cuts at: 0 2477 3036 Size: 2477 559 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 406 727 799 1113 1699 2409 2617 2803 Size: 406 321 72 314 586 710 208 186 Cuts at: 2803 3036 Size: 233 Fragments arranged by size: 710 586 406 321 314 233 208 186 72 Bpu10I CC'TnA_GC Cuts at: 0 1051 1758 2065 2351 3036 Size: 1051 707 307 286 685 Fragments arranged by size: 1051 707 685 307 286 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 257 1089 2113 3036 Size: 257 832 1024 923 Fragments arranged by size: 1024 923 832 257 BsaI GGTCTCn'nnnn_ Cuts at: 0 765 1876 3036 Size: 765 1111 1160 Fragments arranged by size: 1160 1111 765 BsaAI yAC'GTr Cuts at: 0 687 2181 3036 Size: 687 1494 855 Fragments arranged by size: 1494 855 687 BsaHI Gr'CG_yC Cuts at: 0 1293 3036 Size: 1293 1743 BsaWI w'CCGG_w Cuts at: 0 1185 2505 3036 Size: 1185 1320 531 Fragments arranged by size: 1320 1185 531 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 86 116 608 638 685 715 1570 1600 Size: 86 30 492 30 47 30 855 30 Cuts at: 1600 3036 Size: 1436 Fragments arranged by size: 1436 855 492 86 47 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 220 403 2164 3036 Size: 49 171 183 1761 872 Fragments arranged by size: 1761 872 183 171 49 BseYI C'CCAG_C Cuts at: 0 63 923 1089 2135 2550 2700 2761 2891 Size: 63 860 166 1046 415 150 61 130 Cuts at: 2891 3036 Size: 145 Fragments arranged by size: 1046 860 415 166 150 145 130 63 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 874 1079 1115 2228 2824 3007 3036 Size: 523 351 205 36 1113 596 183 29 Fragments arranged by size: 1113 596 523 351 205 183 36 29 BsiEI CG_ry'CG Cuts at: 0 2867 3036 Size: 2867 169 BsiHKAI G_wGCw'C Cuts at: 0 281 320 659 841 888 1202 1565 1829 Size: 281 39 339 182 47 314 363 264 Cuts at: 1829 3036 Size: 1207 Fragments arranged by size: 1207 363 339 314 281 264 182 47 39 BsmI GAATG_Cn' Cuts at: 0 569 3036 Size: 569 2467 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1284 3036 Size: 1284 1752 Bsp1286I G_dGCh'C Cuts at: 0 70 281 320 409 562 659 841 888 Size: 70 211 39 89 153 97 182 47 Cuts at: 888 1075 1202 1227 1565 1699 1752 1829 1845 Size: 187 127 25 338 134 53 77 16 Cuts at: 1845 2019 2401 2520 3036 Size: 174 382 119 516 Fragments arranged by size: 516 382 338 211 187 182 174 153 134 127 119 97 89 77 70 53 47 39 25 16 BspEI T'CCGG_A Cuts at: 0 1185 3036 Size: 1185 1851 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 81 208 350 1051 2906 2955 3036 Size: 81 127 142 701 1855 49 81 Fragments arranged by size: 1855 701 142 127 81 81 49 BsrBI CCG'CTC Cuts at: 0 1533 3036 Size: 1533 1503 BsrDI GCAATG_nn' Cuts at: 0 2555 3036 Size: 2555 481 BsrFI r'CCGG_y Cuts at: 0 914 2505 3036 Size: 914 1591 531 Fragments arranged by size: 1591 914 531 BssSI C'ACGA_G Cuts at: 0 460 3036 Size: 460 2576 BstAPI GCAn_nnn'nTGC Cuts at: 0 1102 1495 1833 2220 3036 Size: 1102 393 338 387 816 Fragments arranged by size: 1102 816 393 387 338 BstBI TT'CG_AA Cuts at: 0 19 3036 Size: 19 3017 BstEII G'GTnAC_C Cuts at: 0 2873 3036 Size: 2873 163 BstXI CCAn_nnnn'nTGG Cuts at: 0 105 150 1462 1994 2215 3036 Size: 105 45 1312 532 221 821 Fragments arranged by size: 1312 821 532 221 105 45 BstYI r'GATC_y Cuts at: 0 933 1548 2831 3036 Size: 933 615 1283 205 Fragments arranged by size: 1283 933 615 205 Bsu36I CC'TnA_GG Cuts at: 0 1214 2905 3036 Size: 1214 1691 131 Fragments arranged by size: 1691 1214 131 BtgI C'CryG_G Cuts at: 0 909 2623 3036 Size: 909 1714 413 Fragments arranged by size: 1714 909 413 BtsI GCAGTG_nn' Cuts at: 0 861 886 1062 1265 1672 1713 1843 3005 Size: 861 25 176 203 407 41 130 1162 Cuts at: 3005 3036 Size: 31 Fragments arranged by size: 1162 861 407 203 176 130 41 31 25 DraIII CAC_nnn'GTG Cuts at: 0 413 3036 Size: 413 2623 DrdI GACnn_nn'nnGTC Cuts at: 0 2784 3036 Size: 2784 252 EaeI y'GGCC_r Cuts at: 0 140 912 916 1082 2022 2864 3036 Size: 140 772 4 166 940 842 172 Fragments arranged by size: 940 842 772 172 166 140 4 EagI C'GGCC_G Cuts at: 0 2864 3036 Size: 2864 172 EarI CTCTTCn'nnn_ Cuts at: 0 995 1007 1450 1870 3036 Size: 995 12 443 420 1166 Fragments arranged by size: 1166 995 443 420 12 EcoICRI GAG'CTC Cuts at: 0 279 657 839 1200 3036 Size: 279 378 182 361 1836 Fragments arranged by size: 1836 378 361 279 182 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 406 418 727 782 799 1006 1113 1138 Size: 406 12 309 55 17 207 107 25 Cuts at: 1138 1431 1687 1699 1975 2281 2409 2428 2617 Size: 293 256 12 276 306 128 19 189 Cuts at: 2617 2746 2770 2788 2803 3036 Size: 129 24 18 15 233 Fragments arranged by size: 406 309 306 293 276 256 233 207 189 129 128 107 55 25 24 19 18 17 15 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 871 3036 Size: 871 2165 EcoO109I rG'GnC_Cy Cuts at: 0 102 123 339 456 507 701 972 1029 Size: 102 21 216 117 51 194 271 57 Cuts at: 1029 1451 1503 1971 2119 2398 2777 3036 Size: 422 52 468 148 279 379 259 Fragments arranged by size: 468 422 379 279 271 259 216 194 148 117 102 57 52 51 21 FseI GG_CCGG'CC Cuts at: 0 918 3036 Size: 918 2118 HaeII r_GCGC'y Cuts at: 0 1319 1422 1638 3036 Size: 1319 103 216 1398 Fragments arranged by size: 1398 1319 216 103 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 86 118 661 685 693 717 1968 2000 Size: 86 32 543 24 8 24 1251 32 Cuts at: 2000 2703 2735 3036 Size: 703 32 301 Fragments arranged by size: 1251 703 543 301 86 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 0 1401 2511 3036 Size: 1401 1110 525 Fragments arranged by size: 1401 1110 525 KpnI G_GTAC'C Cuts at: 0 2758 3036 Size: 2758 278 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 233 329 1893 3036 Size: 233 96 1564 1143 Fragments arranged by size: 1564 1143 233 96 MscI TGG'CCA Cuts at: 0 142 1084 2024 3036 Size: 142 942 940 1012 Fragments arranged by size: 1012 942 940 142 MslI CAynn'nnrTG Cuts at: 0 323 422 908 1568 3036 Size: 323 99 486 660 1468 Fragments arranged by size: 1468 660 486 323 99 MspA1I CmG'CkG Cuts at: 0 54 396 535 1382 1495 1762 2061 2191 Size: 54 342 139 847 113 267 299 130 Cuts at: 2191 2575 2761 2931 2961 3036 Size: 384 186 170 30 75 Fragments arranged by size: 847 384 342 299 267 186 170 139 130 113 75 54 30 NaeI GCC'GGC Cuts at: 0 916 3036 Size: 916 2120 NcoI C'CATG_G Cuts at: 0 909 3036 Size: 909 2127 NgoMIV G'CCGG_C Cuts at: 0 914 3036 Size: 914 2122 NheI G'CTAG_C Cuts at: 0 2473 3036 Size: 2473 563 NsiI A_TGCA'T Cuts at: 0 2216 3036 Size: 2216 820 NspI r_CATG'y Cuts at: 0 1732 3036 Size: 1732 1304 PasI CC'CwG_GG Cuts at: 0 555 929 3036 Size: 555 374 2107 Fragments arranged by size: 2107 555 374 PciI A'CATG_T Cuts at: 0 1728 3036 Size: 1728 1308 PflMI CCAn_nnn'nTGG Cuts at: 0 203 363 828 1966 2142 2292 3036 Size: 203 160 465 1138 176 150 744 Fragments arranged by size: 1138 744 465 203 176 160 150 PfoI T'CCnGG_A Cuts at: 0 1129 3036 Size: 1129 1907 PmlI CAC'GTG Cuts at: 0 687 3036 Size: 687 2349 PpuMI rG'GwC_Cy Cuts at: 0 102 339 507 701 972 1451 1971 2119 Size: 102 237 168 194 271 479 520 148 Cuts at: 2119 2777 3036 Size: 658 259 Fragments arranged by size: 658 520 479 271 259 237 194 168 148 102 PshAI GACnn'nnGTC Cuts at: 0 792 3036 Size: 792 2244 PspOMI G'GGCC_C Cuts at: 0 1841 2397 3036 Size: 1841 556 639 Fragments arranged by size: 1841 639 556 PstI C_TGCA'G Cuts at: 0 122 1491 1767 1838 1881 3036 Size: 122 1369 276 71 43 1155 Fragments arranged by size: 1369 1155 276 122 71 43 PvuII CAG'CTG Cuts at: 0 54 396 535 1495 1762 2061 2191 2575 Size: 54 342 139 960 267 299 130 384 Cuts at: 2575 2761 2931 3036 Size: 186 170 105 Fragments arranged by size: 960 384 342 299 267 186 170 139 130 105 54 SacI G_AGCT'C Cuts at: 0 281 659 841 1202 3036 Size: 281 378 182 361 1834 Fragments arranged by size: 1834 378 361 281 182 SanDI GG'GwC_CC Cuts at: 0 701 3036 Size: 701 2335 SapI GCTCTTCn'nnn_ Cuts at: 0 995 1007 3036 Size: 995 12 2029 Fragments arranged by size: 2029 995 12 SbfI CC_TGCA'GG Cuts at: 0 122 3036 Size: 122 2914 SfcI C'TryA_G Cuts at: 0 118 953 1487 1712 1743 1763 1834 1877 Size: 118 835 534 225 31 20 71 43 Cuts at: 1877 2567 3036 Size: 690 469 Fragments arranged by size: 835 690 534 469 225 118 71 43 31 20 SfiI GGCCn_nnn'nGGCC Cuts at: 0 122 139 2591 3036 Size: 122 17 2452 445 Fragments arranged by size: 2452 445 122 17 SmaI CCC'GGG Cuts at: 0 454 970 1501 3036 Size: 454 516 531 1535 Fragments arranged by size: 1535 531 516 454 SmlI C'TyrA_G Cuts at: 0 272 1068 2092 3036 Size: 272 796 1024 944 Fragments arranged by size: 1024 944 796 272 SnaBI TAC'GTA Cuts at: 0 2181 3036 Size: 2181 855 StuI AGG'CCT Cuts at: 0 116 268 877 1741 2585 3036 Size: 116 152 609 864 844 451 Fragments arranged by size: 864 844 609 451 152 116 StyI C'CwwG_G Cuts at: 0 909 1472 2141 3036 Size: 909 563 669 895 Fragments arranged by size: 909 895 669 563 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 151 1081 1672 3036 Size: 151 930 591 1364 Fragments arranged by size: 1364 930 591 151 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 608 640 3036 Size: 608 32 2396 Fragments arranged by size: 2396 608 32 XbaI T'CTAG_A Cuts at: 0 2316 3036 Size: 2316 720 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 562 907 926 1479 3036 Size: 562 345 19 553 1557 Fragments arranged by size: 1557 562 553 345 19 XmaI C'CCGG_G Cuts at: 0 452 968 1499 3036 Size: 452 516 531 1537 Fragments arranged by size: 1537 531 516 452 XmnI GAAnn'nnTTC Cuts at: 0 2921 3036 Size: 2921 115 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AgeI AleI AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BfrBI BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI SbfI SfcI SfiI SmaI SmlI SnaBI StuI StyI TaqII TstI XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AflII AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BbeI BciVI BclI BglII BmgBI BplI BsaBI BsiWI BspHI BsrGI BssHII BstZ17I BtgZI ClaI CspCI DraI EciI EcoRI EcoRV FalI FspI FspAI HindIII HpaI KasI MfeI MluI NarI NdeI NotI NruI PacI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SexAI SfoI SgfI SgrAI SpeI SphI SrfI SspI SwaI TatI Tth111I XhoI ZraI