(Circular) (Six-base) MAPSORT of: pFN21AE1897.seq Check: 3663 from: 1 to: 7114 pFN21AE1897 7114 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4924 278 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4457 1937 Size: 2520 4594 Acc65I G'GTAC_C Cuts at: 2495 3470 4435 2495 Size: 975 965 5174 Fragments arranged by size: 5174 975 965 AclI AA'CG_TT Cuts at: 1563 5241 5614 1563 Size: 3678 373 3063 Fragments arranged by size: 3678 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 3156 3341 4314 5177 6561 6829 1632 Size: 930 594 185 973 863 1384 268 1917 Fragments arranged by size: 1917 1384 973 930 863 594 268 185 AflII C'TTAA_G Cuts at: 820 1017 3374 3884 820 Size: 197 2357 510 4050 Fragments arranged by size: 4050 2357 510 197 AflIII A'CryG_T Cuts at: 1203 3515 6013 6990 1203 Size: 2312 2498 977 1327 Fragments arranged by size: 2498 2312 1327 977 AgeI A'CCGG_T Cuts at: 2453 5945 2453 Size: 3492 3622 AhdI GACnn_n'nnGTC Cuts at: 5843 5843 Size: 7114 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7114 AlwNI CAG_nnn'CTG Cuts at: 3716 3922 4857 6429 3716 Size: 206 935 1572 4401 Fragments arranged by size: 4401 1572 935 206 ApaI G_GGCC'C Cuts at: 2014 2014 Size: 7114 ApaLI G'TGCA_C Cuts at: 5171 6327 5171 Size: 1156 5958 ApoI r'AATT_y Cuts at: 1493 2690 3464 3749 3845 4423 4649 4801 6910 Size: 1197 774 285 96 578 226 152 2109 Cuts at: 6910 1493 Size: 1697 Fragments arranged by size: 2109 1697 1197 774 578 285 226 152 96 AseI AT'TA_AT Cuts at: 160 1032 4065 5668 160 Size: 872 3033 1603 1606 Fragments arranged by size: 3033 1606 1603 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 AvaI C'yCGr_G Cuts at: 1870 1943 4439 1870 Size: 73 2496 4545 Fragments arranged by size: 4545 2496 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2479 2512 3986 4019 2479 Size: 33 1474 33 5574 Fragments arranged by size: 5574 1474 33 33 BamHI G'GATC_C Cuts at: 4444 4811 4444 Size: 367 6747 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2495 3470 4435 5890 618 Size: 325 217 627 708 975 965 1455 1842 Fragments arranged by size: 1842 1455 975 965 708 627 325 217 BanII G_rGCy'C Cuts at: 1391 2014 2880 4281 4433 1391 Size: 623 866 1401 152 4072 Fragments arranged by size: 4072 1401 866 623 152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3504 3537 4314 4326 928 Size: 971 1605 33 777 12 3716 Fragments arranged by size: 3716 1605 971 777 33 12 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5305 5339 1903 Size: 34 3368 34 3678 Fragments arranged by size: 3678 3368 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2782 5006 6216 2782 Size: 2224 1210 3680 Fragments arranged by size: 3680 2224 1210 BclI T'GATC_A Cuts at: 1548 3417 4083 1548 Size: 1869 666 4579 Fragments arranged by size: 4579 1869 666 BfrBI ATG'CAT Cuts at: 2192 2192 Size: 7114 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5725 136 Size: 107 122 71 631 605 337 3716 1525 Fragments arranged by size: 3716 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 7109 7109 Size: 7114 BlpI GC'TnA_GC Cuts at: 4531 4531 Size: 7114 Bme1580I G_kGCm'C Cuts at: 1790 2014 5175 6331 1790 Size: 224 3161 1156 2573 Fragments arranged by size: 3161 2573 1156 224 BmgBI CAC'GTC Cuts at: 1318 3518 1318 Size: 2200 4914 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3010 4845 5803 Size: 416 53 83 399 24 1593 1835 958 Cuts at: 5803 442 Size: 1753 Fragments arranged by size: 1835 1753 1593 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7114 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2698 2698 Size: 7114 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5774 1239 Size: 4535 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5239 6104 6402 5239 Size: 865 298 5951 Fragments arranged by size: 5951 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3031 5777 882 Size: 617 1532 2746 2219 Fragments arranged by size: 2746 2219 1532 617 BsaAI yAC'GTr Cuts at: 493 1204 3188 4905 493 Size: 711 1984 1717 2702 Fragments arranged by size: 2702 1984 1717 711 BsaBI GATnn'nnATC Cuts at: 7108 7108 Size: 7114 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4921 5303 5938 275 Size: 53 83 186 936 3388 382 635 1451 Fragments arranged by size: 3388 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2453 5547 5945 6219 6366 6777 2453 Size: 3094 398 274 147 411 2790 Fragments arranged by size: 3094 2790 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3166 3196 3916 3946 1178 Size: 30 165 30 1763 30 720 30 4346 Fragments arranged by size: 4346 1763 720 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2888 1186 Size: 1702 5412 BseYI C'CCAG_C Cuts at: 2023 2661 3629 6317 6813 2023 Size: 638 968 2688 496 2324 Fragments arranged by size: 2688 2324 968 638 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6541 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4564 5325 6353 1540 Size: 275 186 2563 761 1028 2301 Fragments arranged by size: 2563 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5175 5260 6331 5175 Size: 85 1071 5958 Fragments arranged by size: 5958 1071 85 BsmI GAATG_Cn' Cuts at: 4233 4625 4718 4233 Size: 392 93 6629 Fragments arranged by size: 6629 392 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2014 2880 4281 4433 5175 5260 6331 Size: 399 224 866 1401 152 742 85 1071 Cuts at: 6331 1391 Size: 2174 Fragments arranged by size: 2174 1401 1071 866 742 399 224 152 85 BspEI T'CCGG_A Cuts at: 6777 6777 Size: 7114 BspHI T'CATG_A Cuts at: 3987 5003 6733 3987 Size: 1016 1730 4368 Fragments arranged by size: 4368 1730 1016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2442 4469 844 Size: 1598 2027 3489 Fragments arranged by size: 3489 2027 1598 BsrBI CCG'CTC Cuts at: 1394 1823 5001 1394 Size: 429 3178 3507 Fragments arranged by size: 3507 3178 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5609 5783 65 Size: 1183 4361 174 1396 Fragments arranged by size: 4361 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2453 5758 5945 1700 Size: 110 643 3305 187 2869 Fragments arranged by size: 3305 2869 643 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7114 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7114 BssSI C'ACGA_G Cuts at: 5174 6186 5174 Size: 1012 6102 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7114 BstBI TT'CG_AA Cuts at: 5918 5918 Size: 7114 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7114 BstYI r'GATC_y Cuts at: 1972 2104 3209 4138 4444 4811 5196 5213 6665 Size: 132 1105 929 306 367 385 17 1452 Cuts at: 6665 6751 6763 7109 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1105 929 385 367 346 306 132 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2880 3111 513 Size: 552 1815 231 4516 Fragments arranged by size: 4516 1815 552 231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6375 Fragments arranged by size: 6375 683 56 BtsI GCAGTG_nn' Cuts at: 4630 5424 5444 4630 Size: 794 20 6300 Fragments arranged by size: 6300 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7114 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7079 DraI TTT'AAA Cuts at: 2529 4418 2529 Size: 1889 5225 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2053 6121 809 Size: 507 737 4068 1802 Fragments arranged by size: 4068 1802 737 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2557 2581 3948 Size: 54 1425 50 275 18 727 24 1367 Cuts at: 3948 4561 5450 7079 8 Size: 613 889 1629 43 Fragments arranged by size: 1629 1425 1367 889 727 613 275 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 4561 1537 Size: 275 2749 4090 Fragments arranged by size: 4090 2749 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2469 2901 3360 3755 4269 4290 5044 Size: 741 375 432 459 395 514 21 754 Cuts at: 5044 1353 Size: 3423 Fragments arranged by size: 3423 754 741 514 459 432 395 375 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2596 5701 6075 6221 387 Size: 1064 138 1007 3105 374 146 1280 Fragments arranged by size: 3105 1280 1064 1007 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2562 3156 3341 4314 5177 5774 6561 Size: 393 930 594 185 973 863 597 787 Cuts at: 6561 6829 1239 Size: 268 1524 Fragments arranged by size: 1524 973 930 863 787 597 594 393 268 185 EcoO109I rG'GnC_Cy Cuts at: 2010 2011 3739 2010 Size: 1 1728 5385 Fragments arranged by size: 5385 1728 1 EcoRI G'AATT_C Cuts at: 4423 4801 4423 Size: 378 6736 EcoRV GAT'ATC Cuts at: 3019 3019 Size: 7114 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2251 2251 Size: 7114 FspI TGC'GCA Cuts at: 5620 5620 Size: 7114 HaeII r_GCGC'y Cuts at: 1678 3922 6261 1678 Size: 2244 2339 2531 Fragments arranged by size: 2531 2339 2244 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 3638 3670 3916 Size: 32 135 32 974 32 1060 32 246 Cuts at: 3916 3948 5755 5787 5829 5861 1373 Size: 32 1807 32 42 32 2626 Fragments arranged by size: 2626 1807 1060 974 246 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2251 2565 4458 4704 669 Size: 988 281 313 314 1893 246 3079 Fragments arranged by size: 3079 1893 988 314 313 281 246 HindIII A'AGCT_T Cuts at: 748 3072 3392 3482 748 Size: 2324 320 90 4380 Fragments arranged by size: 4380 2324 320 90 HpaI GTT'AAC Cuts at: 2251 4704 2251 Size: 2453 4661 KpnI G_GTAC'C Cuts at: 2499 3474 4439 2499 Size: 975 965 5174 Fragments arranged by size: 5174 975 965 MfeI C'AATT_G Cuts at: 4713 4713 Size: 7114 MluI A'CGCG_T Cuts at: 6990 6990 Size: 7114 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2157 2202 3581 6228 6412 1305 Size: 852 45 1379 2647 184 2007 Fragments arranged by size: 2647 2007 1379 852 184 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 2559 2583 3950 10 Size: 54 1425 343 727 24 1367 3174 Fragments arranged by size: 3174 1425 1367 727 343 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 5072 5431 5590 518 Size: 648 3906 359 159 2042 Fragments arranged by size: 3906 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4081 4397 4530 5207 6355 6600 1606 Size: 2475 316 133 677 1148 245 2120 Fragments arranged by size: 2475 2120 1148 677 316 245 133 NcoI C'CATG_G Cuts at: 513 1065 2880 3111 513 Size: 552 1815 231 4516 Fragments arranged by size: 4516 1815 552 231 NdeI CA'TA_TG Cuts at: 387 4207 387 Size: 3820 3294 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7114 NotI GC'GGCC_GC Cuts at: 4561 4561 Size: 7114 NsiI A_TGCA'T Cuts at: 2194 2194 Size: 7114 NspI r_CATG'y Cuts at: 1132 1229 2444 4096 4472 6017 1132 Size: 97 1215 1652 376 1545 2229 Fragments arranged by size: 2229 1652 1545 1215 376 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7114 PciI A'CATG_T Cuts at: 6013 6013 Size: 7114 PflMI CCAn_nnn'nTGG Cuts at: 1108 3586 1108 Size: 2478 4636 PmeI GTTT'AAAC Cuts at: 4418 4418 Size: 7114 PmlI CAC'GTG Cuts at: 3188 3188 Size: 7114 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5183 5215 6696 6728 5183 Size: 32 1481 32 5569 Fragments arranged by size: 5569 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7114 PsiI TTA'TAA Cuts at: 4684 4684 Size: 7114 PspOMI G'GGCC_C Cuts at: 2010 2010 Size: 7114 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2728 2760 2728 Size: 32 7082 PstI C_TGCA'G Cuts at: 830 2938 4466 830 Size: 2108 1528 3478 Fragments arranged by size: 3478 2108 1528 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7114 PvuII CAG'CTG Cuts at: 4397 4397 Size: 7114 SalI G'TCGA_C Cuts at: 1936 4456 1936 Size: 2520 4594 SapI GCTCTTCn'nnn_ Cuts at: 4269 4269 Size: 7114 SbfI CC_TGCA'GG Cuts at: 4466 4466 Size: 7114 ScaI AGT'ACT Cuts at: 5362 7074 5362 Size: 1712 5402 SfcI C'TryA_G Cuts at: 826 1045 2934 4392 4462 5597 6278 6469 826 Size: 219 1889 1458 70 1135 681 191 1471 Fragments arranged by size: 1889 1471 1458 1135 681 219 191 70 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7114 SgrAI Cr'CCGG_yG Cuts at: 1700 2453 1700 Size: 753 6361 SmaI CCC'GGG Cuts at: 1872 4441 1872 Size: 2569 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3374 3884 5218 6119 6381 820 Size: 197 926 1431 510 1334 901 262 1553 Fragments arranged by size: 1553 1431 1334 926 901 510 262 197 SnaBI TAC'GTA Cuts at: 493 4905 493 Size: 4412 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7114 SphI G_CATG'C Cuts at: 1132 4472 1132 Size: 3340 3774 SspI AAT'ATT Cuts at: 5 52 2816 5038 5 Size: 47 2764 2222 2081 Fragments arranged by size: 2764 2222 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7114 StyI C'CwwG_G Cuts at: 513 1065 2491 2880 3111 3780 4553 513 Size: 552 1426 389 231 669 773 3074 Fragments arranged by size: 3074 1426 773 669 552 389 231 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2008 2047 4164 5141 5158 5311 5496 Size: 1255 428 39 2117 977 17 153 185 Cuts at: 5496 5941 325 Size: 445 1498 Fragments arranged by size: 2117 1498 1255 977 445 428 185 153 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2149 5360 7072 Size: 274 80 33 51 1443 172 3211 1712 Cuts at: 7072 96 Size: 138 Fragments arranged by size: 3211 1712 1443 274 172 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3166 3198 7074 7106 3166 Size: 32 3876 32 3174 Fragments arranged by size: 3876 3174 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7114 XbaI T'CTAG_A Cuts at: 4450 4450 Size: 7114 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2476 2476 Size: 7114 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7114 XmaI C'CCGG_G Cuts at: 1870 4439 1870 Size: 2569 4545 XmnI GAAnn'nnTTC Cuts at: 2169 3764 5243 2169 Size: 1595 1479 4040 Fragments arranged by size: 4040 1595 1479 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4922 276 Size: 53 83 186 936 3388 2468 Fragments arranged by size: 3388 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI