Restriction map for pFN21AE1897
 (Linear) (Six-base) MAPSORT of: KIEE1897.seq  Check: 1359  from: 1  to: 2409



KIEE1897.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:50  ..

Acc65I G'GTAC_C

Cuts at:      0     490    1465    2409 
   Size:        490     975     944

  Fragments arranged by size: 

                 975     944     490
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557    1151    1336    2309    2409 
   Size:        557     594     185     973     100

  Fragments arranged by size: 

                 973     594     557     185     100
 
AflII C'TTAA_G

Cuts at:      0    1369    1879    2409 
   Size:       1369     510     530

  Fragments arranged by size: 

                1369     530     510
 
AflIII A'CryG_T

Cuts at:      0    1510    2409 
   Size:       1510     899
 
AgeI A'CCGG_T

Cuts at:      0     448    2409 
   Size:        448    1961
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1711    1917    2409 
   Size:       1711     206     492

  Fragments arranged by size: 

                1711     492     206
 
ApaI G_GGCC'C

Cuts at:      0       9    2409 
   Size:          9    2400
 
ApoI r'AATT_y

Cuts at:      0     685    1459    1744    1840    2409 
   Size:        685     774     285      96     569

  Fragments arranged by size: 

                 774     685     569     285      96
 
AseI AT'TA_AT

Cuts at:      0    2060    2409 
   Size:       2060     349
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     474     507    1981    2014    2409 
   Size:        474      33    1474      33     395

  Fragments arranged by size: 

                1474     474     395      33      33
 
BanI G'GyrC_C

Cuts at:      0     490    1465    2409 
   Size:        490     975     944

  Fragments arranged by size: 

                 975     944     490
 
BanII G_rGCy'C

Cuts at:      0       9     875    2276    2409 
   Size:          9     866    1401     133

  Fragments arranged by size: 

                1401     866     133       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1499    1532    2309    2321    2409 
   Size:       1499      33     777      12      88

  Fragments arranged by size: 

                1499     777      88      33      12
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     777    2409 
   Size:        777    1632
 
BclI T'GATC_A

Cuts at:      0    1412    2078    2409 
   Size:       1412     666     331

  Fragments arranged by size: 

                1412     666     331
 
BfrBI ATG'CAT

Cuts at:      0     187    2409 
   Size:        187    2222
 
Bme1580I G_kGCm'C

Cuts at:      0       9    2409 
   Size:          9    2400
 
BmgBI CAC'GTC

Cuts at:      0    1513    2409 
   Size:       1513     896
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1005    2409 
   Size:       1005    1404
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     693    2409 
   Size:        693    1716
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1026    2409 
   Size:       1026    1383
 
BsaAI yAC'GTr

Cuts at:      0    1183    2409 
   Size:       1183    1226
 
BsaWI w'CCGG_w

Cuts at:      0     448    2409 
   Size:        448    1961
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1161    1191    1911    1941    2409 
   Size:       1161      30     720      30     468

  Fragments arranged by size: 

                1161     720     468      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     883    2409 
   Size:        883    1526
 
BseYI C'CCAG_C

Cuts at:      0      18     656    1624    2409 
   Size:         18     638     968     785

  Fragments arranged by size: 

                 968     785     638      18
 
BsmI GAATG_Cn'

Cuts at:      0    2228    2409 
   Size:       2228     181
 
Bsp1286I G_dGCh'C

Cuts at:      0       9     875    2276    2409 
   Size:          9     866    1401     133

  Fragments arranged by size: 

                1401     866     133       9
 
BspHI T'CATG_A

Cuts at:      0    1982    2409 
   Size:       1982     427
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     437    2409 
   Size:        437    1972
 
BsrFI r'CCGG_y

Cuts at:      0     448    2409 
   Size:        448    1961
 
BstYI r'GATC_y

Cuts at:      0      99    1204    2133    2409 
   Size:         99    1105     929     276

  Fragments arranged by size: 

                1105     929     276      99
 
BtgI C'CryG_G

Cuts at:      0     875    1106    2409 
   Size:        875     231    1303

  Fragments arranged by size: 

                1303     875     231
 
DraI TTT'AAA

Cuts at:      0     524    2409 
   Size:        524    1885
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      48    2409 
   Size:         48    2361
 
EaeI y'GGCC_r

Cuts at:      0     552     576    1943    2409 
   Size:        552      24    1367     466

  Fragments arranged by size: 

                1367     552     466      24
 
EarI CTCTTCn'nnn_

Cuts at:      0      89     464     896    1355    1750    2264    2285    2409 
   Size:         89     375     432     459     395     514      21     124

  Fragments arranged by size: 

                 514     459     432     395     375     124      89      21
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     591    2409 
   Size:        591    1818
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     557    1151    1336    2309    2409 
   Size:        557     594     185     973     100

  Fragments arranged by size: 

                 973     594     557     185     100
 
EcoO109I rG'GnC_Cy

Cuts at:      0       5       6    1734    2409 
   Size:          5       1    1728     675

  Fragments arranged by size: 

                1728     675       5       1
 
EcoRV GAT'ATC

Cuts at:      0    1014    2409 
   Size:       1014    1395
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     246    2409 
   Size:        246    2163
 
HaeII r_GCGC'y

Cuts at:      0    1917    2409 
   Size:       1917     492
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     541     573    1633    1665    1911    1943    2409 
   Size:        541      32    1060      32     246      32     466

  Fragments arranged by size: 

                1060     541     466     246      32      32      32
 
HincII GTy'rAC

Cuts at:      0     246     560    2409 
   Size:        246     314    1849

  Fragments arranged by size: 

                1849     314     246
 
HindIII A'AGCT_T

Cuts at:      0    1067    1387    1477    2409 
   Size:       1067     320      90     932

  Fragments arranged by size: 

                1067     932     320      90
 
HpaI GTT'AAC

Cuts at:      0     246    2409 
   Size:        246    2163
 
KpnI G_GTAC'C

Cuts at:      0     494    1469    2409 
   Size:        494     975     940

  Fragments arranged by size: 

                 975     940     494
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     197    1576    2409 
   Size:        152      45    1379     833

  Fragments arranged by size: 

                1379     833     152      45
 
MscI TGG'CCA

Cuts at:      0     554     578    1945    2409 
   Size:        554      24    1367     464

  Fragments arranged by size: 

                1367     554     464      24
 
MspA1I CmG'CkG

Cuts at:      0    2076    2392    2409 
   Size:       2076     316      17

  Fragments arranged by size: 

                2076     316      17
 
NcoI C'CATG_G

Cuts at:      0     875    1106    2409 
   Size:        875     231    1303

  Fragments arranged by size: 

                1303     875     231
 
NdeI CA'TA_TG

Cuts at:      0    2202    2409 
   Size:       2202     207
 
NsiI A_TGCA'T

Cuts at:      0     189    2409 
   Size:        189    2220
 
NspI r_CATG'y

Cuts at:      0     439    2091    2409 
   Size:        439    1652     318

  Fragments arranged by size: 

                1652     439     318
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1581    2409 
   Size:       1581     828
 
PmlI CAC'GTG

Cuts at:      0    1183    2409 
   Size:       1183    1226
 
PspOMI G'GGCC_C

Cuts at:      0       5    2409 
   Size:          5    2404
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     723     755    2409 
   Size:        723      32    1654

  Fragments arranged by size: 

                1654     723      32
 
PstI C_TGCA'G

Cuts at:      0     933    2409 
   Size:        933    1476
 
PvuII CAG'CTG

Cuts at:      0    2392    2409 
   Size:       2392      17
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2264    2409 
   Size:       2264     145
 
SfcI C'TryA_G

Cuts at:      0     929    2387    2409 
   Size:        929    1458      22

  Fragments arranged by size: 

                1458     929      22
 
SgrAI Cr'CCGG_yG

Cuts at:      0     448    2409 
   Size:        448    1961
 
SmlI C'TyrA_G

Cuts at:      0    1369    1879    2409 
   Size:       1369     510     530

  Fragments arranged by size: 

                1369     530     510
 
SspI AAT'ATT

Cuts at:      0     811    2409 
   Size:        811    1598
 
StyI C'CwwG_G

Cuts at:      0     486     875    1106    1775    2409 
   Size:        486     389     231     669     634

  Fragments arranged by size: 

                 669     634     486     389     231
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       3      42    2159    2409 
   Size:          3      39    2117     250

  Fragments arranged by size: 

                2117     250      39       3
 
TatI w'GTAC_w

Cuts at:      0     144    2409 
   Size:        144    2265
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1161    1193    2409 
   Size:       1161      32    1216

  Fragments arranged by size: 

                1216    1161      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     471    2409 
   Size:        471    1938
 
XmnI GAAnn'nnTTC

Cuts at:      0     164    1759    2409 
   Size:        164    1595     650

  Fragments arranged by size: 

                1595     650     164
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     AgeI    AlwNI     ApaI     ApoI
     AseI     BaeI     BanI    BanII     BbsI    BciVI     BclI    BfrBI
 Bme1580I    BmgBI     BmrI     BplI     BsaI    BsaAI    BsaWI    BsaXI
    BseRI    BseYI     BsmI Bsp1286I    BspHI    BspMI    BsrFI    BstYI
     BtgI     DraI     DrdI     EaeI     EarI     EciI  Eco57MI EcoO109I
    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MmeI     MscI   MspA1I     NcoI     NdeI     NsiI     NspI    PflMI
     PmlI   PspOMI     PsrI     PstI    PvuII     SapI     SfcI    SgrAI
     SmlI     SspI     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AhdI     AleI     AlfI
     AloI    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI     BbeI
    BbvCI     BcgI     BglI    BglII     BlpI     BmtI     BpmI   Bpu10I
    BpuEI    BsaBI    BsaHI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
   DraIII     EagI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI
     KasI     MfeI     MluI     MslI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PciI     PfoI     PmeI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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