Restriction map for
pFN21AE1978
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AvrII BbeI
BciVI BfaI BfrI BglII BlnI
BsaBI BsaHI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FseI HgaI Hin1I HindIII HpaI
KasI KpnI MaeI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccII 1064 CGCG CG^CG
AcsI 1552 AAATTC [GA]^AATT[CT]
ApoI 1552 AAATTC [GA]^AATT[CT]
Asp700I 1317 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1606 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 716 TGCGCA TGC^GCA
BamHI 950 GGATCC G^GATCC
BbrPI 1039 CACGTG CAC^GTG
BbsI 480 GAAGAC GAAGAC(2/6)
BbvCI 1341 GCTGAGG CCTCAGC(-5/-2)
BcgI 1168 CGAGACCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1491 TGATCA T^GATCA
BglI 161 GCCGCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1196 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1196 GCTCAGC GC^T[ACGT]AGC
BpuAI 480 GAAGAC GAAGAC(2/6)
BsaAI 1039 CACGTG [CT]AC^GT[GA]
BsaI 1169 GAGACC GGTCTC(1/5)
BsaWI 1646 ACCGGA [AT]^CCGG[AT]
BsiEI 1135 CGGCCG CG[GA][CT]^CG
BsmAI 1169 GAGAC GTCTC(1/5)
BsrBI 162 CCGCTC CCGCTC(-3/-3)
BsrDI 422 GCAATG GCAATG(2/0)
BstAPI 1038 GCACGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1064 CGCG CG^CG
BstXI 291 CCAGGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 950 GGATCC [GA]^GATC[CT]
CelII 1196 GCTCAGC GC^T[ACGT]AGC
DraIII 1353 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 382 GACTGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1135 CGGCCG C^GGCCG
EclXI 1135 CGGCCG C^GGCCG
Eco52I 1135 CGGCCG C^GGCCG
FbaI 1491 TGATCA T^GATCA
FspI 716 TGCGCA TGC^GCA
HincII 790 GTCAAC GT[CT]^[GA]AC
HindII 790 GTCAAC GT[CT]^[GA]AC
KspI 1063 CCGCGG CCGC^GG
MflI 950 GGATCC [GA]^GATC[CT]
MseI 932 TTAA T^TAA
MslI 1175 CATGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1064 CGCG CG^CG
NcoI 1191 CCATGG C^CATGG
NspI 429 GCATGT [GA]CATG^[CT]
PflFI 1606 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1039 CACGTG CAC^GTG
PmlI 1039 CACGTG CAC^GTG
PstI 1128 CTGCAG CTGCA^G
PvuII 1246 CAGCTG CAG^CTG
SacII 1063 CCGCGG CCGC^GG
ScaI 971 AGTACT AGT^ACT
SmaI 353 CCCGGG CCC^GGG
StuI 830 AGGCCT AGG^CCT
TaqI 779 TCGA T^CGA
TfiI 1536 GAATC G^A[AT]TC
Tru9I 932 TTAA T^TAA
Tsp509I 1553 AATT ^AATT
Tth111I 1606 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 779 TCGA T^CGA
XcmI 338 CCACTGAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 950 GGATCC [GA]^GATC[CT]
XmaCI 353 CCCGGG C^CCGGG
XmaI 353 CCCGGG C^CCGGG
XmnI 1317 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AocI 636 CCTCAGG CC^T[ACGT]AGG
1431 CCTGAGG
AspHI 1350 GAGCAC G[AT]GC[AT]^C
1408 GTGCTC
BsiHKAI 1350 GAGCAC G[AT]GC[AT]^C
1408 GTGCTC
BsrFI 442 GCCGGC [GA]^CCGG[CT]
1133 GCCGGC
BstEII 1203 GGTCACC G^GT[ACGT]ACC
1254 GGTGACC
BstPI 1203 GGTCACC G^GT[ACGT]ACC
1254 GGTGACC
Bsu36I 636 CCTCAGG CC^T[ACGT]AGG
1431 CCTGAGG
Cfr10I 442 GCCGGC [GA]^CCGG[CT]
1133 GCCGGC
EarI 1321 CTCTTC CTCTTC(1/4)
1158 GAAGAG
Eco81I 636 CCTCAGG CC^T[ACGT]AGG
1431 CCTGAGG
EcoNI 237 CCTACAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
632 CCTACCTCAGG
EcoO65I 1203 GGTCACC G^GT[ACGT]ACC
1254 GGTGACC
Ksp632I 1321 CTCTTC CTCTTC(1/4)
1158 GAAGAG
NaeI 442 GCCGGC GCC^GGC
1133 GCCGGC
NgoMIV 442 GCCGGC G^CCGGC
1133 GCCGGC
PpuMI 593 GGGACCT [GA]G^G[AT]CC[CT]
1058 AGGACCC
SapI 1158 GAAGAGC GCTCTTC(1/4)
1320 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaI AvaII BalI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TseI
Tsp45I TspRI