(Circular) (Six-base) MAPSORT of: pFN21AE1978.seq Check: 7286 from: 1 to: 6370 pFN21AE1978 6370 bp With 182 enzymes: SgfI * June 30, 2019 15:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2228 2735 3611 2228 Size: 507 876 4987 Fragments arranged by size: 4987 876 507 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4180 278 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3713 1937 Size: 1776 4594 Acc65I G'GTAC_C Cuts at: 3691 3691 Size: 6370 AclI AA'CG_TT Cuts at: 1563 4497 4870 1563 Size: 2934 373 3063 Fragments arranged by size: 3063 2934 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2367 2494 4433 5817 6085 1632 Size: 735 127 1939 1384 268 1917 Fragments arranged by size: 1939 1917 1384 735 268 127 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6173 AflIII A'CryG_T Cuts at: 1203 5269 6246 1203 Size: 4066 977 1327 Fragments arranged by size: 4066 1327 977 AgeI A'CCGG_T Cuts at: 5201 5201 Size: 6370 AhdI GACnn_n'nnGTC Cuts at: 5099 5099 Size: 6370 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6370 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6370 AlwNI CAG_nnn'CTG Cuts at: 2266 3106 3346 4113 5685 2266 Size: 840 240 767 1572 2951 Fragments arranged by size: 2951 1572 840 767 240 ApaI G_GGCC'C Cuts at: 2296 2593 2970 3094 3435 2296 Size: 297 377 124 341 5231 Fragments arranged by size: 5231 377 341 297 124 ApaLI G'TGCA_C Cuts at: 4427 5583 4427 Size: 1156 5214 ApoI r'AATT_y Cuts at: 1493 3557 3679 3905 4057 6166 1493 Size: 2064 122 226 152 2109 1697 Fragments arranged by size: 2109 2064 1697 226 152 122 AseI AT'TA_AT Cuts at: 160 1032 4924 160 Size: 872 3892 1606 Fragments arranged by size: 3892 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 AvaI C'yCGr_G Cuts at: 1870 1943 2170 2358 2594 2969 3171 3695 1870 Size: 73 227 188 236 375 202 524 4545 Fragments arranged by size: 4545 524 375 236 227 202 188 73 BamHI G'GATC_C Cuts at: 2955 3700 4067 2955 Size: 745 367 5258 Fragments arranged by size: 5258 745 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 2252 2663 3488 3599 Size: 325 217 627 429 36 411 825 111 Cuts at: 3599 3691 5146 618 Size: 92 1455 1842 Fragments arranged by size: 1842 1455 825 627 429 411 325 217 111 92 36 BanII G_rGCy'C Cuts at: 1391 2177 2296 2303 2593 2751 2970 3094 3121 Size: 786 119 7 290 158 219 124 27 Cuts at: 3121 3170 3435 3689 1391 Size: 49 265 254 4072 Fragments arranged by size: 4072 786 290 265 254 219 158 124 119 49 27 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2492 928 Size: 971 593 4806 Fragments arranged by size: 4806 971 593 BbvCI CC'TCA_GC Cuts at: 3347 3347 Size: 6370 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3162 3196 4561 4595 1903 Size: 34 1225 34 1365 34 3678 Fragments arranged by size: 3678 1365 1225 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4262 5472 4262 Size: 1210 5160 BclI T'GATC_A Cuts at: 1548 3496 1548 Size: 1948 4422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2172 4981 136 Size: 107 122 71 631 605 500 2809 1525 Fragments arranged by size: 2809 1525 631 605 500 122 107 71 BglII A'GATC_T Cuts at: 6365 6365 Size: 6370 BlpI GC'TnA_GC Cuts at: 3202 3787 3202 Size: 585 5785 Bme1580I G_kGCm'C Cuts at: 1790 2255 2296 2593 2970 3094 3149 3435 4431 Size: 465 41 297 377 124 55 286 996 Cuts at: 4431 5587 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 996 465 377 297 286 124 55 41 BmgBI CAC'GTC Cuts at: 1318 2152 2407 1318 Size: 834 255 5281 Fragments arranged by size: 5281 834 255 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2177 4101 5059 Size: 416 53 83 399 24 760 1924 958 Cuts at: 5059 442 Size: 1753 Fragments arranged by size: 1924 1753 958 760 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6370 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2995 2995 Size: 6370 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2243 3145 3275 3627 5030 1239 Size: 1004 902 130 352 1403 2579 Fragments arranged by size: 2579 1403 1004 902 352 130 Bpu10I CC'TnA_GC Cuts at: 2415 3347 2415 Size: 932 5438 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4495 5360 5658 4495 Size: 865 298 5207 Fragments arranged by size: 5207 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3168 5033 882 Size: 617 1669 1865 2219 Fragments arranged by size: 2219 1865 1669 617 BsaAI yAC'GTr Cuts at: 493 1204 3046 4161 493 Size: 711 1842 1115 2702 Fragments arranged by size: 2702 1842 1115 711 BsaBI GATnn'nnATC Cuts at: 6364 6364 Size: 6370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4177 4559 5194 275 Size: 53 83 186 936 2644 382 635 1451 Fragments arranged by size: 2644 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3651 4803 5201 5475 5622 6033 3651 Size: 1152 398 274 147 411 3988 Fragments arranged by size: 3988 1152 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6145 Fragments arranged by size: 6145 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2858 3100 3609 1186 Size: 826 846 242 509 3947 Fragments arranged by size: 3947 846 826 509 242 BseYI C'CCAG_C Cuts at: 2628 2689 3249 5573 6069 2628 Size: 61 560 2324 496 2929 Fragments arranged by size: 2929 2324 560 496 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2206 2555 3026 1163 Size: 573 470 349 471 4507 Fragments arranged by size: 4507 573 471 470 349 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3143 3820 4581 5609 1540 Size: 275 186 1142 677 761 1028 2301 Fragments arranged by size: 2301 1142 1028 761 677 275 186 BsiHKAI G_wGCw'C Cuts at: 3359 3417 4431 4516 5587 3359 Size: 58 1014 85 1071 4142 Fragments arranged by size: 4142 1071 1014 85 58 BsmI GAATG_Cn' Cuts at: 3881 3974 3881 Size: 93 6277 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2255 2296 2303 2593 2751 2970 Size: 399 387 78 41 7 290 158 219 Cuts at: 2970 3094 3121 3149 3170 3359 3417 3435 3689 Size: 124 27 28 21 189 58 18 254 Cuts at: 3689 4431 4516 5587 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 290 254 219 189 158 124 85 78 58 41 28 27 21 18 7 BspEI T'CCGG_A Cuts at: 6033 6033 Size: 6370 BspHI T'CATG_A Cuts at: 4259 5989 4259 Size: 1730 4640 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2228 2610 2735 2952 3436 3611 3725 844 Size: 1384 382 125 217 484 175 114 3489 Fragments arranged by size: 3489 1384 484 382 217 175 125 114 BsrBI CCG'CTC Cuts at: 1394 1823 2169 4257 1394 Size: 429 346 2088 3507 Fragments arranged by size: 3507 2088 429 346 BsrDI GCAATG_nn' Cuts at: 65 1248 2434 4865 5039 65 Size: 1183 1186 2431 174 1396 Fragments arranged by size: 2431 1396 1186 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2447 3138 5014 5201 1700 Size: 110 637 691 1876 187 2869 Fragments arranged by size: 2869 1876 691 637 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6370 BssSI C'ACGA_G Cuts at: 4430 5442 4430 Size: 1012 5358 BstAPI GCAn_nnn'nTGC Cuts at: 794 3049 794 Size: 2255 4115 BstBI TT'CG_AA Cuts at: 5174 5174 Size: 6370 BstEII G'GTnAC_C Cuts at: 1184 3208 3259 1184 Size: 2024 51 4295 Fragments arranged by size: 4295 2024 51 BstXI CCAn_nnnn'nTGG Cuts at: 2303 2303 Size: 6370 BstYI r'GATC_y Cuts at: 1972 2955 3700 4067 4452 4469 5921 6007 6019 Size: 983 745 367 385 17 1452 86 12 Cuts at: 6019 6365 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 983 745 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2642 3437 2642 Size: 795 5575 BtgI C'CryG_G Cuts at: 513 1065 2004 2809 3068 3196 3306 513 Size: 552 939 805 259 128 110 3577 Fragments arranged by size: 3577 939 805 552 259 128 110 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5631 Fragments arranged by size: 5631 683 56 BtsI GCAGTG_nn' Cuts at: 2712 3300 3886 4680 4700 2712 Size: 588 586 794 20 4382 Fragments arranged by size: 4382 794 588 586 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6370 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6335 DraI TTT'AAA Cuts at: 3674 3674 Size: 6370 DraIII CAC_nnn'GTG Cuts at: 3363 3363 Size: 6370 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2393 5377 809 Size: 507 1077 2984 1802 Fragments arranged by size: 2984 1802 1077 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2445 2695 Size: 54 1425 50 275 18 303 312 250 Cuts at: 2695 2920 3140 3337 3420 3547 3817 4706 6335 Size: 225 220 197 83 127 270 889 1629 Cuts at: 6335 8 Size: 43 Fragments arranged by size: 1629 1425 889 312 303 275 270 250 225 220 197 127 83 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3140 3817 1537 Size: 275 1328 677 4090 Fragments arranged by size: 4090 1328 677 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3158 3332 4300 1353 Size: 1805 174 968 3423 Fragments arranged by size: 3423 1805 968 174 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2153 4957 5331 5477 387 Size: 1064 138 564 2804 374 146 1280 Fragments arranged by size: 2804 1280 1064 564 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2367 2494 3145 3275 3627 4433 Size: 393 611 124 127 651 130 352 806 Cuts at: 4433 5030 5817 6085 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 806 787 651 611 597 393 352 268 130 127 124 EcoNI CCTnn'n_nnAGG Cuts at: 2246 2641 2246 Size: 395 5975 EcoO109I rG'GnC_Cy Cuts at: 2589 2590 2599 3064 3090 3091 3241 3280 3431 Size: 1 9 465 26 1 150 39 151 Cuts at: 3431 3432 2589 Size: 1 5527 Fragments arranged by size: 5527 465 151 150 39 26 9 1 1 1 EcoRI G'AATT_C Cuts at: 3679 4057 3679 Size: 378 5992 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3346 3346 Size: 6370 FspI TGC'GCA Cuts at: 2723 4876 2723 Size: 2153 4217 HaeII r_GCGC'y Cuts at: 1678 2266 2279 2317 2902 5517 1678 Size: 588 13 38 585 2615 2531 Fragments arranged by size: 2615 2531 588 585 38 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3446 3478 5011 5043 5085 Size: 32 135 32 1874 32 1533 32 42 Cuts at: 5085 5117 1373 Size: 32 2626 Fragments arranged by size: 2626 1874 1533 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2797 3714 3960 669 Size: 988 281 859 917 246 3079 Fragments arranged by size: 3079 988 917 859 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6370 HpaI GTT'AAC Cuts at: 3960 3960 Size: 6370 KpnI G_GTAC'C Cuts at: 3695 3695 Size: 6370 MfeI C'AATT_G Cuts at: 3969 3969 Size: 6370 MluI A'CGCG_T Cuts at: 6246 6246 Size: 6370 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2679 5484 5668 1305 Size: 1374 2805 184 2007 Fragments arranged by size: 2805 2007 1374 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3339 3422 3549 10 Size: 54 1425 343 1507 83 127 2831 Fragments arranged by size: 2831 1507 1425 343 127 83 54 MslI CAynn'nnrTG Cuts at: 518 1166 3184 4328 4687 4846 518 Size: 648 2018 1144 359 159 2042 Fragments arranged by size: 2042 2018 1144 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2569 3070 3206 3253 3786 4463 5611 5856 Size: 963 501 136 47 533 677 1148 245 Cuts at: 5856 1606 Size: 2120 Fragments arranged by size: 2120 1148 963 677 533 501 245 136 47 NaeI GCC'GGC Cuts at: 2449 3140 2449 Size: 691 5679 NcoI C'CATG_G Cuts at: 513 1065 2004 3196 513 Size: 552 939 1192 3687 Fragments arranged by size: 3687 1192 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6370 NgoMIV G'CCGG_C Cuts at: 2447 3138 2447 Size: 691 5679 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6370 NotI GC'GGCC_GC Cuts at: 3817 3817 Size: 6370 NspI r_CATG'y Cuts at: 1132 1229 2438 3728 5273 1132 Size: 97 1209 1290 1545 2229 Fragments arranged by size: 2229 1545 1290 1209 97 PasI CC'CwG_GG Cuts at: 1348 2296 1348 Size: 948 5422 PciI A'CATG_T Cuts at: 5269 5269 Size: 6370 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6370 PfoI T'CCnGG_A Cuts at: 3643 3643 Size: 6370 PmeI GTTT'AAAC Cuts at: 3674 3674 Size: 6370 PmlI CAC'GTG Cuts at: 3046 3046 Size: 6370 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4439 4471 5952 5984 4439 Size: 32 1481 32 4825 Fragments arranged by size: 4825 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2599 3064 2599 Size: 465 5905 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6370 PsiI TTA'TAA Cuts at: 3940 3940 Size: 6370 PspOMI G'GGCC_C Cuts at: 2292 2589 2966 3090 3431 2292 Size: 297 377 124 341 5231 Fragments arranged by size: 5231 377 341 297 124 PstI C_TGCA'G Cuts at: 830 3137 3722 830 Size: 2307 585 3478 Fragments arranged by size: 3478 2307 585 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6370 PvuII CAG'CTG Cuts at: 3253 3253 Size: 6370 SacII CC_GC'GG Cuts at: 3071 3071 Size: 6370 SalI G'TCGA_C Cuts at: 1936 3712 1936 Size: 1776 4594 SapI GCTCTTCn'nnn_ Cuts at: 3158 3332 3158 Size: 174 6196 SbfI CC_TGCA'GG Cuts at: 3722 3722 Size: 6370 ScaI AGT'ACT Cuts at: 2978 4618 6330 2978 Size: 1640 1712 3018 Fragments arranged by size: 3018 1712 1640 SfcI C'TryA_G Cuts at: 826 1045 2243 2539 3133 3718 4853 5534 5725 Size: 219 1198 296 594 585 1135 681 191 Cuts at: 5725 826 Size: 1471 Fragments arranged by size: 1471 1198 1135 681 594 585 296 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6370 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6370 SmaI CCC'GGG Cuts at: 1872 2360 3697 1872 Size: 488 1337 4545 Fragments arranged by size: 4545 1337 488 SmlI C'TyrA_G Cuts at: 820 1017 1943 4474 5375 5637 820 Size: 197 926 2531 901 262 1553 Fragments arranged by size: 2531 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4161 493 Size: 3668 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6370 SphI G_CATG'C Cuts at: 1132 3728 1132 Size: 2596 3774 SspI AAT'ATT Cuts at: 5 52 4294 5 Size: 47 4242 2081 Fragments arranged by size: 4242 2081 47 StuI AGG'CCT Cuts at: 1517 2837 1517 Size: 1320 5050 StyI C'CwwG_G Cuts at: 513 1065 2004 2832 3196 3484 3809 513 Size: 552 939 828 364 288 325 3074 Fragments arranged by size: 3074 939 828 552 364 325 288 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2642 2681 3230 4397 4414 4567 4752 Size: 1255 1062 39 549 1167 17 153 185 Cuts at: 4752 5197 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1167 1062 549 445 185 153 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2502 2976 4616 Size: 274 80 33 51 1443 525 474 1640 Cuts at: 4616 6328 96 Size: 1712 138 Fragments arranged by size: 1712 1640 1443 525 474 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2184 2216 6330 6362 2184 Size: 32 4114 32 2192 Fragments arranged by size: 4114 2192 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3614 1316 Size: 2298 4072 XbaI T'CTAG_A Cuts at: 3706 3706 Size: 6370 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2350 Size: 6370 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6370 XmaI C'CCGG_G Cuts at: 1870 2358 3695 1870 Size: 488 1337 4545 Fragments arranged by size: 4545 1337 488 XmnI GAAnn'nnTTC Cuts at: 3326 4499 3326 Size: 1173 5197 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4178 276 Size: 53 83 186 936 2644 2468 Fragments arranged by size: 2644 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI