(Circular) (Six-base) MAPSORT of: pFN21AE2263.seq Check: 2505 from: 1 to: 5860 pFN21AE2263 5860 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2731 3203 1937 Size: 794 472 4594 Fragments arranged by size: 4594 794 472 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 2382 3987 4360 1563 Size: 819 1605 373 3063 Fragments arranged by size: 3063 1605 819 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3923 5307 5575 1632 Size: 2291 1384 268 1917 Fragments arranged by size: 2291 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 3136 4759 5736 1203 Size: 1933 1623 977 1327 Fragments arranged by size: 1933 1623 1327 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 4589 4589 Size: 5860 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2682 2859 1184 Size: 1498 177 4185 Fragments arranged by size: 4185 1498 177 AlwNI CAG_nnn'CTG Cuts at: 3032 3603 5175 3032 Size: 571 1572 3717 Fragments arranged by size: 3717 1572 571 ApaI G_GGCC'C Cuts at: 2037 2683 2037 Size: 646 5214 ApaLI G'TGCA_C Cuts at: 3917 5073 3917 Size: 1156 4704 ApoI r'AATT_y Cuts at: 1493 2279 2760 3169 3395 3547 5656 1493 Size: 786 481 409 226 152 2109 1697 Fragments arranged by size: 2109 1697 786 481 409 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2637 2893 3185 1870 Size: 73 694 256 292 4545 Fragments arranged by size: 4545 694 292 256 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2154 2187 2154 Size: 33 5827 BamHI G'GATC_C Cuts at: 2817 3190 3557 2817 Size: 373 367 5120 Fragments arranged by size: 5120 373 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3181 4636 618 Size: 325 217 627 1394 1455 1842 Fragments arranged by size: 1842 1455 1394 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 2683 2902 2960 3082 3179 1391 Size: 646 646 219 58 122 97 4072 Fragments arranged by size: 4072 646 646 219 122 97 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2988 928 Size: 971 1089 3800 Fragments arranged by size: 3800 1089 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4051 4085 1903 Size: 34 50 34 2030 34 3678 Fragments arranged by size: 3678 2030 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2182 3752 4962 2182 Size: 1570 1210 3080 Fragments arranged by size: 3080 1570 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5860 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2906 4471 136 Size: 107 122 71 631 605 1234 1565 1525 Fragments arranged by size: 1565 1525 1234 631 605 122 107 71 BglII A'GATC_T Cuts at: 5855 5855 Size: 5860 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 2037 2683 3921 5077 1790 Size: 247 646 1238 1156 2573 Fragments arranged by size: 2573 1238 1156 646 247 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2701 3591 4549 Size: 416 53 83 399 24 1284 890 958 Cuts at: 4549 442 Size: 1753 Fragments arranged by size: 1753 1284 958 890 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2376 2578 2597 2974 3094 4520 1239 Size: 1137 202 19 377 120 1426 2579 Fragments arranged by size: 2579 1426 1137 377 202 120 19 Bpu10I CC'TnA_GC Cuts at: 3025 3051 3081 3025 Size: 26 30 5804 Fragments arranged by size: 5804 30 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3985 4850 5148 3985 Size: 865 298 4697 Fragments arranged by size: 4697 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4523 882 Size: 617 3024 2219 Fragments arranged by size: 3024 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3139 3651 493 Size: 711 1935 512 2702 Fragments arranged by size: 2702 1935 711 512 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3667 4049 4684 275 Size: 53 83 186 936 2134 382 635 1451 Fragments arranged by size: 2134 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4293 4691 4965 5112 5523 4293 Size: 398 274 147 411 4630 Fragments arranged by size: 4630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5635 Fragments arranged by size: 5635 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 1186 Size: 1483 4377 BseYI C'CCAG_C Cuts at: 2374 2675 2812 2974 5063 5559 2374 Size: 301 137 162 2089 496 2675 Fragments arranged by size: 2675 2089 496 301 162 137 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2204 1163 Size: 573 468 4819 Fragments arranged by size: 4819 573 468 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3921 4006 5077 3921 Size: 85 1071 4704 Fragments arranged by size: 4704 1071 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2683 2902 2960 3082 3179 3921 Size: 399 247 646 219 58 122 97 742 Cuts at: 3921 4006 5077 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 646 399 247 219 122 97 85 58 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 2094 3749 5479 2094 Size: 1655 1730 2475 Fragments arranged by size: 2475 1730 1655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3215 844 Size: 2371 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3747 1394 Size: 429 1924 3507 Fragments arranged by size: 3507 1924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2248 4355 4529 65 Size: 1183 1000 2107 174 1396 Fragments arranged by size: 2107 1396 1183 1000 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3095 4504 4691 1700 Size: 110 1285 1409 187 2869 Fragments arranged by size: 2869 1409 1285 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 2022 2024 2026 1925 Size: 97 2 2 5759 Fragments arranged by size: 5759 97 2 2 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5860 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 2887 1184 Size: 1703 4157 BstYI r'GATC_y Cuts at: 1972 2817 3190 3557 3942 3959 5411 5497 5509 Size: 845 373 367 385 17 1452 86 12 Cuts at: 5509 5855 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 845 385 373 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3045 3045 Size: 5860 BtgI C'CryG_G Cuts at: 513 1065 2045 513 Size: 552 980 4328 Fragments arranged by size: 4328 980 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 3376 4170 4190 3376 Size: 794 20 5046 Fragments arranged by size: 5046 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DraIII CAC_nnn'GTG Cuts at: 2227 3142 2227 Size: 915 4945 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2745 3097 3118 Size: 54 1425 50 275 18 915 352 21 Cuts at: 3118 3307 4196 5825 8 Size: 189 889 1629 43 Fragments arranged by size: 1629 1425 915 889 352 275 189 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2332 3790 1353 Size: 979 1458 3423 Fragments arranged by size: 3423 1458 979 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2376 2578 2597 2974 3094 3923 4520 Size: 393 744 202 19 377 120 829 597 Cuts at: 4520 5307 5575 1239 Size: 787 268 1524 Fragments arranged by size: 1524 829 787 744 597 393 377 268 202 120 19 EcoO109I rG'GnC_Cy Cuts at: 2033 2629 2679 3029 3036 2033 Size: 596 50 350 7 4857 Fragments arranged by size: 4857 596 350 50 7 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 5007 1678 Size: 3329 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4501 4533 4575 4607 1373 Size: 32 135 32 2929 32 42 32 2626 Fragments arranged by size: 2929 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2800 4974 5158 1305 Size: 1495 2174 184 2007 Fragments arranged by size: 2174 2007 1495 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2747 10 Size: 54 1425 343 915 3123 Fragments arranged by size: 3123 1425 915 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2241 3818 4177 4336 518 Size: 648 1075 1577 359 159 2042 Fragments arranged by size: 2042 1577 1075 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2598 2952 3276 3953 5101 5346 1606 Size: 441 551 354 324 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 551 441 354 324 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5308 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 3218 4763 1132 Size: 97 1989 1545 2229 Fragments arranged by size: 2229 1989 1545 97 PasI CC'CwG_GG Cuts at: 1348 2140 1348 Size: 792 5068 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PmlI CAC'GTG Cuts at: 3139 3139 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3029 3036 3029 Size: 7 5853 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PspOMI G'GGCC_C Cuts at: 2033 2679 2033 Size: 646 5214 PstI C_TGCA'G Cuts at: 830 3212 830 Size: 2382 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2598 2952 2598 Size: 354 5506 SacII CC_GC'GG Cuts at: 2048 2048 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 3019 3208 4343 5024 5215 826 Size: 219 1974 189 1135 681 191 1471 Fragments arranged by size: 1974 1471 1135 681 219 191 189 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3187 1872 Size: 1315 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3964 4865 5127 820 Size: 197 926 2021 901 262 1553 Fragments arranged by size: 2021 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 2722 1517 Size: 1205 4655 StyI C'CwwG_G Cuts at: 513 1065 2203 3299 513 Size: 552 1138 1096 3074 Fragments arranged by size: 3074 1138 1096 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3887 3904 4057 4242 4687 325 Size: 1255 2307 17 153 185 445 1498 Fragments arranged by size: 2307 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4106 5818 96 Size: 274 80 33 51 1443 2129 1712 138 Fragments arranged by size: 2129 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5820 5852 5820 Size: 32 5828 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3185 1870 Size: 1315 4545 XmnI GAAnn'nnTTC Cuts at: 2149 2431 3989 2149 Size: 282 1558 4020 Fragments arranged by size: 4020 1558 282 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI