Restriction map for pFN21AE2079
 (Linear) (Six-base) MAPSORT of: KIEE2079.seq  Check: 6047  from: 1  to: 2256



KIEE2079.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:54  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     837    1714    2256 
   Size:        837     877     542

  Fragments arranged by size: 

                 877     837     542
 
AfeI AGC'GCT

Cuts at:      0     745    2256 
   Size:        745    1511
 
AflII C'TTAA_G

Cuts at:      0    1885    2256 
   Size:       1885     371
 
AflIII A'CryG_T

Cuts at:      0    1400    2256 
   Size:       1400     856
 
AleI CACnn'nnGTG

Cuts at:      0     186    2256 
   Size:        186    2070
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     274    1536    2256 
   Size:        274    1262     720

  Fragments arranged by size: 

                1262     720     274
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     919     951    2256 
   Size:        919      32    1305

  Fragments arranged by size: 

                1305     919      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     162     274    1796    2226    2256 
   Size:        162     112    1522     430      30

  Fragments arranged by size: 

                1522     430     162     112      30
 
ApaI G_GGCC'C

Cuts at:      0       7    1485    2151    2256 
   Size:          7    1478     666     105

  Fragments arranged by size: 

                1478     666     105       7
 
ApoI r'AATT_y

Cuts at:      0    1594    2241    2256 
   Size:       1594     647      15

  Fragments arranged by size: 

                1594     647      15
 
AvaI C'yCGr_G

Cuts at:      0      90     938    2256 
   Size:         90     848    1318

  Fragments arranged by size: 

                1318     848      90
 
BanI G'GyrC_C

Cuts at:      0      97    2256 
   Size:         97    2159
 
BanII G_rGCy'C

Cuts at:      0       7     126     376     605     905    1485    2151    2256 
   Size:          7     119     250     229     300     580     666     105

  Fragments arranged by size: 

                 666     580     300     250     229     119     105       7
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     548     712    2256 
   Size:        548     164    1544

  Fragments arranged by size: 

                1544     548     164
 
BbvCI CC'TCA_GC

Cuts at:      0     109    2215    2256 
   Size:        109    2106      41

  Fragments arranged by size: 

                2106     109      41
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     525     559    2256 
   Size:        525      34    1697

  Fragments arranged by size: 

                1697     525      34
 
BclI T'GATC_A

Cuts at:      0     956    2256 
   Size:        956    1300
 
BglI GCCn_nnn'nGGC

Cuts at:      0     158     401    1768    2256 
   Size:        158     243    1367     488

  Fragments arranged by size: 

                1367     488     243     158
 
Bme1580I G_kGCm'C

Cuts at:      0       7     664    1485    2151    2256 
   Size:          7     657     821     666     105

  Fragments arranged by size: 

                 821     666     657     105       7
 
BmgBI CAC'GTC

Cuts at:      0     645    2256 
   Size:        645    1611
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     481    1013    1575    2256 
   Size:        481     532     562     681

  Fragments arranged by size: 

                 681     562     532     481
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     846    1941    2256 
   Size:        846    1095     315

  Fragments arranged by size: 

                1095     846     315
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     609    1278    1684    2256 
   Size:        609     669     406     572

  Fragments arranged by size: 

                 669     609     572     406
 
Bpu10I CC'TnA_GC

Cuts at:      0     109    2215    2256 
   Size:        109    2106      41

  Fragments arranged by size: 

                2106     109      41
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1769    2074    2256 
   Size:       1769     305     182

  Fragments arranged by size: 

                1769     305     182
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     236    1462    1562    1773    2256 
   Size:        236    1226     100     211     483

  Fragments arranged by size: 

                1226     483     236     211     100
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     553     583     919     949    1528    1558    2119    2149 
   Size:        553      30     336      30     579      30     561      30

Cuts at:   2149    2256 
   Size:        107

  Fragments arranged by size: 

                 579     561     553     336     107      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     522    1057    2256 
   Size:        522     535    1199

  Fragments arranged by size: 

                1199     535     522
 
BseYI C'CCAG_C

Cuts at:      0     119     174     309    2093    2256 
   Size:        119      55     135    1784     163

  Fragments arranged by size: 

                1784     163     135     119      55
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1318    1734    2256 
   Size:       1318     416     522

  Fragments arranged by size: 

                1318     522     416
 
BsiHKAI G_wGCw'C

Cuts at:      0     548    2256 
   Size:        548    1708
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     126     376     548     605     664     905    1485 
   Size:          7     119     250     172      57      59     241     580

Cuts at:   1485    2151    2256 
   Size:        666     105

  Fragments arranged by size: 

                 666     580     250     241     172     119     105      59
                  57       7
 
BspHI T'CATG_A

Cuts at:      0     990    1043    2256 
   Size:        990      53    1213

  Fragments arranged by size: 

                1213     990      53
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     776    1480    2256 
   Size:        776     704     776

  Fragments arranged by size: 

                 776     776     704
 
BsrBI CCG'CTC

Cuts at:      0     564    2058    2256 
   Size:        564    1494     198

  Fragments arranged by size: 

                1494     564     198
 
BsrDI GCAATG_nn'

Cuts at:      0     339    1518    2256 
   Size:        339    1179     738

  Fragments arranged by size: 

                1179     738     339
 
BssSI C'ACGA_G

Cuts at:      0    2174    2256 
   Size:       2174      82
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     682     748    1236    2256 
   Size:        682      66     488    1020

  Fragments arranged by size: 

                1020     682     488      66
 
BstYI r'GATC_y

Cuts at:      0     848    1181    2256 
   Size:        848     333    1075

  Fragments arranged by size: 

                1075     848     333
 
Bsu36I CC'TnA_GG

Cuts at:      0     638    2256 
   Size:        638    1618
 
BtgI C'CryG_G

Cuts at:      0     114     304     323    2256 
   Size:        114     190      19    1933

  Fragments arranged by size: 

                1933     190     114      19
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1544    2256 
   Size:       1544     712
 
BtsI GCAGTG_nn'

Cuts at:      0     447     485    2001    2256 
   Size:        447      38    1516     255

  Fragments arranged by size: 

                1516     447     255      38
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1125    1160    2256 
   Size:       1125      35    1096

  Fragments arranged by size: 

                1125    1096      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1501    2256 
   Size:       1501     755
 
EaeI y'GGCC_r

Cuts at:      0     444    2256 
   Size:        444    1812
 
EarI CTCTTCn'nnn_

Cuts at:      0    1195    2200    2256 
   Size:       1195    1005      56

  Fragments arranged by size: 

                1195    1005      56
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     609     837    1278    1684    1714    2256 
   Size:        609     228     441     406      30     542

  Fragments arranged by size: 

                 609     542     441     406     228      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2214    2256 
   Size:       2214      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0     159     314    1481    1482    1503    2000    2147    2148 
   Size:        159     155    1167       1      21     497     147       1

Cuts at:   2148    2163    2256 
   Size:         15      93

  Fragments arranged by size: 

                1167     497     159     155     147      93      21      15
                   1       1
 
EcoRI G'AATT_C

Cuts at:      0    1594    2256 
   Size:       1594     662
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1615    2256 
   Size:       1615     641
 
HaeII r_GCGC'y

Cuts at:      0     747     830    2256 
   Size:        747      83    1426

  Fragments arranged by size: 

                1426     747      83
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1094    1117    1126    1149    1264    1296    1500    1532 
   Size:       1094      23       9      23     115      32     204      32

Cuts at:   1532    1561    1593    2082    2114    2119    2151    2256 
   Size:         29      32     489      32       5      32     105

  Fragments arranged by size: 

                1094     489     204     115     105      32      32      32
                  32      32      29      23      23       9       5
 
HincII GTy'rAC

Cuts at:      0    1167    2256 
   Size:       1167    1089
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    1125    1517    2256 
   Size:        776     349     392     739

  Fragments arranged by size: 

                 776     739     392     349
 
MscI TGG'CCA

Cuts at:      0     446    2256 
   Size:        446    1810
 
MslI CAynn'nnrTG

Cuts at:      0     186     995    1234    1811    2256 
   Size:        186     809     239     577     445

  Fragments arranged by size: 

                 809     577     445     239     186
 
MspA1I CmG'CkG

Cuts at:      0     200     424    1162    2256 
   Size:        200     224     738    1094

  Fragments arranged by size: 

                1094     738     224     200
 
NcoI C'CATG_G

Cuts at:      0     114    2256 
   Size:        114    2142
 
NdeI CA'TA_TG

Cuts at:      0    1352    2256 
   Size:       1352     904
 
NspI r_CATG'y

Cuts at:      0     408     681    1108    1306    1404    1444    2205    2256 
   Size:        408     273     427     198      98      40     761      51

  Fragments arranged by size: 

                 761     427     408     273     198      98      51      40
            
 
PasI CC'CwG_GG

Cuts at:      0     156     657    1624    2256 
   Size:        156     501     967     632

  Fragments arranged by size: 

                 967     632     501     156
 
PciI A'CATG_T

Cuts at:      0    1400    2256 
   Size:       1400     856
 
PfoI T'CCnGG_A

Cuts at:      0     773    1661    2256 
   Size:        773     888     595

  Fragments arranged by size: 

                 888     773     595
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     919     951    2256 
   Size:        919      32    1305

  Fragments arranged by size: 

                1305     919      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     314    1503    2000    2163    2256 
   Size:        314    1189     497     163      93

  Fragments arranged by size: 

                1189     497     314     163      93
 
PshAI GACnn'nnGTC

Cuts at:      0    1960    2256 
   Size:       1960     296
 
PspOMI G'GGCC_C

Cuts at:      0       3    1481    2147    2256 
   Size:          3    1478     666     109

  Fragments arranged by size: 

                1478     666     109       3
 
PstI C_TGCA'G

Cuts at:      0    1319    2256 
   Size:       1319     937
 
PvuII CAG'CTG

Cuts at:      0     424    1162    2256 
   Size:        424     738    1094

  Fragments arranged by size: 

                1094     738     424
 
SanDI GG'GwC_CC

Cuts at:      0    2000    2256 
   Size:       2000     256
 
SexAI A'CCwGG_T

Cuts at:      0    1337    2256 
   Size:       1337     919
 
SfcI C'TryA_G

Cuts at:      0    1315    1797    2256 
   Size:       1315     482     459

  Fragments arranged by size: 

                1315     482     459
 
SmaI CCC'GGG

Cuts at:      0     940    2256 
   Size:        940    1316
 
SmlI C'TyrA_G

Cuts at:      0    1784    1885    2053    2256 
   Size:       1784     101     168     203

  Fragments arranged by size: 

                1784     203     168     101
 
SphI G_CATG'C

Cuts at:      0    1306    2205    2256 
   Size:       1306     899      51

  Fragments arranged by size: 

                1306     899      51
 
StyI C'CwwG_G

Cuts at:      0     114    2115    2256 
   Size:        114    2001     141

  Fragments arranged by size: 

                2001     141     114
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     316     715    1132    1738    2256 
   Size:        316     399     417     606     518

  Fragments arranged by size: 

                 606     518     417     399     316
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     553     585    1896    1928    2256 
   Size:        553      32    1311      32     328

  Fragments arranged by size: 

                1311     553     328      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     514    2256 
   Size:        514    1742
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1389    2256 
   Size:       1389     867
 
XmaI C'CCGG_G

Cuts at:      0     938    2256 
   Size:        938    1318
 

 Enzymes that do cut:

     AcuI     AfeI    AflII   AflIII     AleI     AlfI     AloI    AlwNI
     ApaI     ApoI     AvaI     BanI    BanII     BbsI    BbvCI     BcgI
     BclI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BssSI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DrdI     EaeI     EarI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NspI     PasI     PciI
     PfoI     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII    SanDI
    SexAI     SfcI     SmaI     SmlI     SphI     StyI    TaqII     TstI
  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AgeI     AhdI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI
    BfrBI    BglII     BlpI     BmtI    BsaAI    BsaBI    BsaHI    BsaWI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I     ClaI     DraI   DraIII     EagI
     EciI  EcoICRI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PmeI     PmlI     PsiI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TatI     XbaI     XhoI     XmnI
     ZraI
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