(Linear) (Six-base) MAPSORT of: KIEE2079.seq Check: 6047 from: 1 to: 2256 KIEE2079.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 837 1714 2256 Size: 837 877 542 Fragments arranged by size: 877 837 542 AfeI AGC'GCT Cuts at: 0 745 2256 Size: 745 1511 AflII C'TTAA_G Cuts at: 0 1885 2256 Size: 1885 371 AflIII A'CryG_T Cuts at: 0 1400 2256 Size: 1400 856 AleI CACnn'nnGTG Cuts at: 0 186 2256 Size: 186 2070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 274 1536 2256 Size: 274 1262 720 Fragments arranged by size: 1262 720 274 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 919 951 2256 Size: 919 32 1305 Fragments arranged by size: 1305 919 32 AlwNI CAG_nnn'CTG Cuts at: 0 162 274 1796 2226 2256 Size: 162 112 1522 430 30 Fragments arranged by size: 1522 430 162 112 30 ApaI G_GGCC'C Cuts at: 0 7 1485 2151 2256 Size: 7 1478 666 105 Fragments arranged by size: 1478 666 105 7 ApoI r'AATT_y Cuts at: 0 1594 2241 2256 Size: 1594 647 15 Fragments arranged by size: 1594 647 15 AvaI C'yCGr_G Cuts at: 0 90 938 2256 Size: 90 848 1318 Fragments arranged by size: 1318 848 90 BanI G'GyrC_C Cuts at: 0 97 2256 Size: 97 2159 BanII G_rGCy'C Cuts at: 0 7 126 376 605 905 1485 2151 2256 Size: 7 119 250 229 300 580 666 105 Fragments arranged by size: 666 580 300 250 229 119 105 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 548 712 2256 Size: 548 164 1544 Fragments arranged by size: 1544 548 164 BbvCI CC'TCA_GC Cuts at: 0 109 2215 2256 Size: 109 2106 41 Fragments arranged by size: 2106 109 41 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 525 559 2256 Size: 525 34 1697 Fragments arranged by size: 1697 525 34 BclI T'GATC_A Cuts at: 0 956 2256 Size: 956 1300 BglI GCCn_nnn'nGGC Cuts at: 0 158 401 1768 2256 Size: 158 243 1367 488 Fragments arranged by size: 1367 488 243 158 Bme1580I G_kGCm'C Cuts at: 0 7 664 1485 2151 2256 Size: 7 657 821 666 105 Fragments arranged by size: 821 666 657 105 7 BmgBI CAC'GTC Cuts at: 0 645 2256 Size: 645 1611 BmrI ACTGGGnnnn_n' Cuts at: 0 481 1013 1575 2256 Size: 481 532 562 681 Fragments arranged by size: 681 562 532 481 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 846 1941 2256 Size: 846 1095 315 Fragments arranged by size: 1095 846 315 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 609 1278 1684 2256 Size: 609 669 406 572 Fragments arranged by size: 669 609 572 406 Bpu10I CC'TnA_GC Cuts at: 0 109 2215 2256 Size: 109 2106 41 Fragments arranged by size: 2106 109 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1769 2074 2256 Size: 1769 305 182 Fragments arranged by size: 1769 305 182 BsaI GGTCTCn'nnnn_ Cuts at: 0 236 1462 1562 1773 2256 Size: 236 1226 100 211 483 Fragments arranged by size: 1226 483 236 211 100 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 553 583 919 949 1528 1558 2119 2149 Size: 553 30 336 30 579 30 561 30 Cuts at: 2149 2256 Size: 107 Fragments arranged by size: 579 561 553 336 107 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 522 1057 2256 Size: 522 535 1199 Fragments arranged by size: 1199 535 522 BseYI C'CCAG_C Cuts at: 0 119 174 309 2093 2256 Size: 119 55 135 1784 163 Fragments arranged by size: 1784 163 135 119 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1318 1734 2256 Size: 1318 416 522 Fragments arranged by size: 1318 522 416 BsiHKAI G_wGCw'C Cuts at: 0 548 2256 Size: 548 1708 Bsp1286I G_dGCh'C Cuts at: 0 7 126 376 548 605 664 905 1485 Size: 7 119 250 172 57 59 241 580 Cuts at: 1485 2151 2256 Size: 666 105 Fragments arranged by size: 666 580 250 241 172 119 105 59 57 7 BspHI T'CATG_A Cuts at: 0 990 1043 2256 Size: 990 53 1213 Fragments arranged by size: 1213 990 53 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 776 1480 2256 Size: 776 704 776 Fragments arranged by size: 776 776 704 BsrBI CCG'CTC Cuts at: 0 564 2058 2256 Size: 564 1494 198 Fragments arranged by size: 1494 564 198 BsrDI GCAATG_nn' Cuts at: 0 339 1518 2256 Size: 339 1179 738 Fragments arranged by size: 1179 738 339 BssSI C'ACGA_G Cuts at: 0 2174 2256 Size: 2174 82 BstXI CCAn_nnnn'nTGG Cuts at: 0 682 748 1236 2256 Size: 682 66 488 1020 Fragments arranged by size: 1020 682 488 66 BstYI r'GATC_y Cuts at: 0 848 1181 2256 Size: 848 333 1075 Fragments arranged by size: 1075 848 333 Bsu36I CC'TnA_GG Cuts at: 0 638 2256 Size: 638 1618 BtgI C'CryG_G Cuts at: 0 114 304 323 2256 Size: 114 190 19 1933 Fragments arranged by size: 1933 190 114 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1544 2256 Size: 1544 712 BtsI GCAGTG_nn' Cuts at: 0 447 485 2001 2256 Size: 447 38 1516 255 Fragments arranged by size: 1516 447 255 38 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1125 1160 2256 Size: 1125 35 1096 Fragments arranged by size: 1125 1096 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1501 2256 Size: 1501 755 EaeI y'GGCC_r Cuts at: 0 444 2256 Size: 444 1812 EarI CTCTTCn'nnn_ Cuts at: 0 1195 2200 2256 Size: 1195 1005 56 Fragments arranged by size: 1195 1005 56 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 609 837 1278 1684 1714 2256 Size: 609 228 441 406 30 542 Fragments arranged by size: 609 542 441 406 228 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 2214 2256 Size: 2214 42 EcoO109I rG'GnC_Cy Cuts at: 0 159 314 1481 1482 1503 2000 2147 2148 Size: 159 155 1167 1 21 497 147 1 Cuts at: 2148 2163 2256 Size: 15 93 Fragments arranged by size: 1167 497 159 155 147 93 21 15 1 1 EcoRI G'AATT_C Cuts at: 0 1594 2256 Size: 1594 662 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1615 2256 Size: 1615 641 HaeII r_GCGC'y Cuts at: 0 747 830 2256 Size: 747 83 1426 Fragments arranged by size: 1426 747 83 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1094 1117 1126 1149 1264 1296 1500 1532 Size: 1094 23 9 23 115 32 204 32 Cuts at: 1532 1561 1593 2082 2114 2119 2151 2256 Size: 29 32 489 32 5 32 105 Fragments arranged by size: 1094 489 204 115 105 32 32 32 32 32 29 23 23 9 5 HincII GTy'rAC Cuts at: 0 1167 2256 Size: 1167 1089 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 776 1125 1517 2256 Size: 776 349 392 739 Fragments arranged by size: 776 739 392 349 MscI TGG'CCA Cuts at: 0 446 2256 Size: 446 1810 MslI CAynn'nnrTG Cuts at: 0 186 995 1234 1811 2256 Size: 186 809 239 577 445 Fragments arranged by size: 809 577 445 239 186 MspA1I CmG'CkG Cuts at: 0 200 424 1162 2256 Size: 200 224 738 1094 Fragments arranged by size: 1094 738 224 200 NcoI C'CATG_G Cuts at: 0 114 2256 Size: 114 2142 NdeI CA'TA_TG Cuts at: 0 1352 2256 Size: 1352 904 NspI r_CATG'y Cuts at: 0 408 681 1108 1306 1404 1444 2205 2256 Size: 408 273 427 198 98 40 761 51 Fragments arranged by size: 761 427 408 273 198 98 51 40 PasI CC'CwG_GG Cuts at: 0 156 657 1624 2256 Size: 156 501 967 632 Fragments arranged by size: 967 632 501 156 PciI A'CATG_T Cuts at: 0 1400 2256 Size: 1400 856 PfoI T'CCnGG_A Cuts at: 0 773 1661 2256 Size: 773 888 595 Fragments arranged by size: 888 773 595 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 919 951 2256 Size: 919 32 1305 Fragments arranged by size: 1305 919 32 PpuMI rG'GwC_Cy Cuts at: 0 314 1503 2000 2163 2256 Size: 314 1189 497 163 93 Fragments arranged by size: 1189 497 314 163 93 PshAI GACnn'nnGTC Cuts at: 0 1960 2256 Size: 1960 296 PspOMI G'GGCC_C Cuts at: 0 3 1481 2147 2256 Size: 3 1478 666 109 Fragments arranged by size: 1478 666 109 3 PstI C_TGCA'G Cuts at: 0 1319 2256 Size: 1319 937 PvuII CAG'CTG Cuts at: 0 424 1162 2256 Size: 424 738 1094 Fragments arranged by size: 1094 738 424 SanDI GG'GwC_CC Cuts at: 0 2000 2256 Size: 2000 256 SexAI A'CCwGG_T Cuts at: 0 1337 2256 Size: 1337 919 SfcI C'TryA_G Cuts at: 0 1315 1797 2256 Size: 1315 482 459 Fragments arranged by size: 1315 482 459 SmaI CCC'GGG Cuts at: 0 940 2256 Size: 940 1316 SmlI C'TyrA_G Cuts at: 0 1784 1885 2053 2256 Size: 1784 101 168 203 Fragments arranged by size: 1784 203 168 101 SphI G_CATG'C Cuts at: 0 1306 2205 2256 Size: 1306 899 51 Fragments arranged by size: 1306 899 51 StyI C'CwwG_G Cuts at: 0 114 2115 2256 Size: 114 2001 141 Fragments arranged by size: 2001 141 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 316 715 1132 1738 2256 Size: 316 399 417 606 518 Fragments arranged by size: 606 518 417 399 316 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 553 585 1896 1928 2256 Size: 553 32 1311 32 328 Fragments arranged by size: 1311 553 328 32 32 Tth111I GACn'n_nGTC Cuts at: 0 514 2256 Size: 514 1742 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1389 2256 Size: 1389 867 XmaI C'CCGG_G Cuts at: 0 938 2256 Size: 938 1318 Enzymes that do cut: AcuI AfeI AflII AflIII AleI AlfI AloI AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NdeI NspI PasI PciI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SanDI SexAI SfcI SmaI SmlI SphI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AgeI AhdI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BglII BlpI BmtI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I ClaI DraI DraIII EagI EciI EcoICRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PflMI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI XhoI XmnI ZraI