################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AvaII AvrII BalI BamHI BbrPI BbsI BclI BfrI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrGI Bst1107I BstAPI BstBI BstXI BstYI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MflI MluNI MscI MunI NaeI NcoI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tsp509I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 22 GTCGAC GT^[AC][GT]AC AccIII 42 TCCGGA T^CCGGA AcyI 1045 GGCGCC G[GA]^CG[CT]C AfaI 1733 GTAC GT^AC Alw44I 118 GTGCAC G^TGCAC AlwI 1768 GATCC GGATC(4/5) AocI 1118 CCTTAGG CC^T[ACGT]AGG ApaLI 118 GTGCAC G^TGCAC AspI 1884 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 284 TGCGCA TGC^GCA BbeI 1045 GGCGCC GGCGC^C BbvCI 929 CCTCAGC CCTCAGC(-5/-2) BlpI 823 GCTCAGC GC^T[ACGT]AGC Bpu1102I 823 GCTCAGC GC^T[ACGT]AGC BsaHI 1045 GGCGCC G[GA]^CG[CT]C BsaWI 42 TCCGGA [AT]^CCGG[AT] BseAI 42 TCCGGA T^CCGGA BsmAI 835 GTCTC GTCTC(1/5) BspEI 42 TCCGGA T^CCGGA BsrBI 1875 CCGCTC CCGCTC(-3/-3) BstEII 1024 GGTAACC G^GT[ACGT]ACC BstPI 1024 GGTAACC G^GT[ACGT]ACC Bsu36I 1118 CCTTAGG CC^T[ACGT]AGG CelII 823 GCTCAGC GC^T[ACGT]AGC DpnII 1768 GATC ^GATC EagI 181 CGGCCG C^GGCCG EclXI 181 CGGCCG C^GGCCG Eco52I 181 CGGCCG C^GGCCG Eco81I 1118 CCTTAGG CC^T[ACGT]AGG EcoO65I 1024 GGTAACC G^GT[ACGT]ACC FspI 284 TGCGCA TGC^GCA Hin1I 1045 GGCGCC G[GA]^CG[CT]C HincII 22 GTCGAC GT[CT]^[GA]AC HindII 22 GTCGAC GT[CT]^[GA]AC KasI 1045 GGCGCC G^GCGCC MboI 1768 GATC ^GATC MroI 42 TCCGGA T^CCGGA MseI 227 TTAA T^TAA NarI 1045 GGCGCC GG^CGCC NdeII 1768 GATC ^GATC NruI 633 TCGCGA TCG^CGA PflFI 1884 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 1733 GTAC GT^AC SacI 1828 GAGCTC GAGCT^C SalI 22 GTCGAC G^TCGAC Sau3AI 1768 GATC ^GATC SfoI 1045 GGCGCC GGC^GCC SmaI 1751 CCCGGG CCC^GGG SphI 265 GCATGC GCATG^C StuI 220 AGGCCT AGG^CCT Tru9I 227 TTAA T^TAA Tth111I 1884 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1751 CCCGGG C^CCGGG XmaI 1751 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflIII 309 ACGCGT A^C[GA][CT]GT 341 ACGCGT AspHI 118 GTGCAC G[AT]GC[AT]^C 1828 GAGCTC AvaI 665 CCCGAG C^[CT]CG[GA]G 1751 CCCGGG BciVI 33 GGATAC GTATCC(6/5) 513 GGATAC BsiHKAI 118 GTGCAC G[AT]GC[AT]^C 1828 GAGCTC BsmFI 485 GGGAC GGGAC(10/14) 1683 GTCCC BsoBI 665 CCCGAG C^[CT]CG[GA]G 1751 CCCGGG BspHI 83 TCATGA T^CATGA 98 TCATGA BsrDI 251 GCAATG GCAATG(2/0) 1634 GCAATG BsrFI 1392 GCCGGT [GA]^CCGG[CT] 1814 ACCGGC BstF5I 1034 CATCC GGATG(2/0) 1597 CATCC Cfr10I 1392 GCCGGT [GA]^CCGG[CT] 1814 ACCGGC DsaI 303 CCGCGG C^C[GA][CT]GG 1620 CCGCGG FokI 1034 CATCC GGATG(9/13) 1597 CATCC HgaI 308 GACGC GACGC(5/10) 311 GCGTC HphI 330 GGTGA GGTGA(8/7) 658 TCACC KspI 303 CCGCGG CCGC^GG 1620 CCGCGG MluI 309 ACGCGT A^CGCGT 341 ACGCGT MslI 78 CACGGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 423 CATCAACGTG NdeI 911 CATATG CA^TATG 1247 CATATG NspI 122 ACATGC [GA]CATG^[CT] 265 GCATGC RcaI 83 TCATGA T^CATGA 98 TCATGA SacII 303 CCGCGG CCGC^GG 1620 CCGCGG Tsp45I 323 GTGAC ^GT[GC]AC 729 GTCAC XcmI 561 CCAGCAGGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1358 CCACTACCCCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AluI AlwNI ApaI Asp700I BanI BanII BbvI BcgI BcnI BfaI BglI BmyI BsaJI BseRI BsgI BsiEI BsiYI BslI Bsp1286I BspMI BsrI BssHII BssKI BssSI BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PflMI PleI PstI PvuII Sau96I ScrFI SfaNI SfcI StyI TaqI TseI TspRI TthHB8I Van91I XmnI