Restriction map for
pFN21AE2048
(Circular) (Six-base) MAPSORT of: pFN21AE2048.seq Check: 6518 from: 1 to: 6712
pFN21AE2048 6712 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4522 278
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2028 4055 1937
Size: 91 2027 4594
Fragments arranged by size:
4594 2027 91
Acc65I G'GTAC_C
Cuts at: 4033 4033
Size: 6712
AclI AA'CG_TT
Cuts at: 1563 4839 5212 1563
Size: 3276 373 3063
Fragments arranged by size:
3276 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4775 6159 6427 1632
Size: 3143 1384 268 1917
Fragments arranged by size:
3143 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6515
AflIII A'CryG_T
Cuts at: 1203 2314 2346 5611 6588 1203
Size: 1111 32 3265 977 1327
Fragments arranged by size:
3265 1327 1111 977 32
AgeI A'CCGG_T
Cuts at: 5543 5543
Size: 6712
AhdI GACnn_n'nnGTC
Cuts at: 5441 5441
Size: 6712
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2669 1184
Size: 1485 5227
AlwNI CAG_nnn'CTG
Cuts at: 2287 2513 3323 4455 6027 2287
Size: 226 810 1132 1572 2972
Fragments arranged by size:
2972 1572 1132 810 226
ApaI G_GGCC'C
Cuts at: 2722 2758 3163 3394 3433 3523 2722
Size: 36 405 231 39 90 5911
Fragments arranged by size:
5911 405 231 90 39 36
ApaLI G'TGCA_C
Cuts at: 2123 4769 5925 2123
Size: 2646 1156 2910
Fragments arranged by size:
2910 2646 1156
ApoI r'AATT_y
Cuts at: 1493 4021 4247 4399 6508 1493
Size: 2528 226 152 2109 1697
Fragments arranged by size:
2528 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 2670 3756 4037 1870
Size: 73 727 1086 281 4545
Fragments arranged by size:
4545 1086 727 281 73
BamHI G'GATC_C
Cuts at: 4042 4409 4042
Size: 367 6345
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2851 3050 3500 4033 5488
Size: 325 217 627 1064 199 450 533 1455
Cuts at: 5488 618
Size: 1842
Fragments arranged by size:
1842 1455 1064 627 533 450 325 217
199
BanII G_rGCy'C
Cuts at: 1391 2722 2758 2929 3004 3142 3163 3196 3394
Size: 1331 36 171 75 138 21 33 198
Cuts at: 3394 3433 3469 3523 3837 3956 4031 1391
Size: 39 36 54 314 119 75 4072
Fragments arranged by size:
4072 1331 314 198 171 138 119 75
75 54 39 36 36 33 21
BbeI G_GCGC'C
Cuts at: 3054 3054
Size: 6712
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5741
BbvCI CC'TCA_GC
Cuts at: 2935 2935
Size: 6712
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2281 2315 2964 2998 3930 3964 4903
Size: 34 344 34 649 34 932 34 939
Cuts at: 4903 4937 1903
Size: 34 3678
Fragments arranged by size:
3678 939 932 649 344 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2032 2512 4604 5814 2032
Size: 480 2092 1210 2930
Fragments arranged by size:
2930 2092 1210 480
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6712
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2753 3302 3453
Size: 107 122 71 631 605 1081 549 151
Cuts at: 3453 3518 3539 3575 3636 5323 136
Size: 65 21 36 61 1687 1525
Fragments arranged by size:
1687 1525 1081 631 605 549 151 122
107 71 65 61 36 21
BglII A'GATC_T
Cuts at: 6707 6707
Size: 6712
BlpI GC'TnA_GC
Cuts at: 2829 4129 2829
Size: 1300 5412
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2722 2758 2854 3163 3394 3433 3523
Size: 337 595 36 96 309 231 39 90
Cuts at: 3523 4773 5929 1790
Size: 1250 1156 2573
Fragments arranged by size:
2573 1250 1156 595 337 309 231 96
90 39 36
BmgBI CAC'GTC
Cuts at: 1318 2332 1318
Size: 1014 5698
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2747 2881 3157
Size: 416 53 83 399 24 1330 134 276
Cuts at: 3157 3235 4443 5401 442
Size: 78 1208 958 1753
Fragments arranged by size:
1753 1330 1208 958 416 399 276 134
83 78 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5372 1239
Size: 4133 2579
Bpu10I CC'TnA_GC
Cuts at: 2935 3003 3141 2935
Size: 68 138 6506
Fragments arranged by size:
6506 138 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4837 5702 6000 4837
Size: 865 298 5549
Fragments arranged by size:
5549 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5375 882
Size: 617 3876 2219
Fragments arranged by size:
3876 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4503 493
Size: 711 3299 2702
Fragments arranged by size:
3299 2702 711
BsaBI GATnn'nnATC
Cuts at: 6706 6706
Size: 6712
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3051 4519 4901 5536
Size: 53 83 186 936 1518 1468 382 635
Cuts at: 5536 275
Size: 1451
Fragments arranged by size:
1518 1468 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2047 5145 5543 5817 5964 6375 2047
Size: 3098 398 274 147 411 2384
Fragments arranged by size:
3098 2384 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6487
Fragments arranged by size:
6487 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2679 3166 1186
Size: 880 613 487 4732
Fragments arranged by size:
4732 880 613 487
BseYI C'CCAG_C
Cuts at: 2513 2700 2721 2757 2928 3216 3393 3411 3594
Size: 187 21 36 171 288 177 18 183
Cuts at: 3594 5915 6411 2513
Size: 2321 496 2814
Fragments arranged by size:
2814 2321 496 288 187 183 177 171
36 21 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 3102 3243 3425 1163
Size: 573 417 949 141 182 4450
Fragments arranged by size:
4450 949 573 417 182 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2189 2644 3820 4162 4923 5951
Size: 275 186 188 455 1176 342 761 1028
Cuts at: 5951 1540
Size: 2301
Fragments arranged by size:
2301 1176 1028 761 455 342 275 188
186
BsiHKAI G_wGCw'C
Cuts at: 2127 3837 4773 4858 5929 2127
Size: 1710 936 85 1071 2910
Fragments arranged by size:
2910 1710 1071 936 85
BsmI GAATG_Cn'
Cuts at: 4223 4316 4223
Size: 93 6619
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2722 2758 2854 2929 3004 3142
Size: 399 337 595 36 96 75 75 138
Cuts at: 3142 3163 3196 3394 3433 3469 3523 3837 3956
Size: 21 33 198 39 36 54 314 119
Cuts at: 3956 4031 4773 4858 5929 1391
Size: 75 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 595 399 337 314 198
138 119 96 85 75 75 75 54
39 36 36 33 21
BspEI T'CCGG_A
Cuts at: 2047 6375 2047
Size: 4328 2384
BspHI T'CATG_A
Cuts at: 2088 2103 4601 6331 2088
Size: 15 2498 1730 2469
Fragments arranged by size:
2498 2469 1730 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3298 3647 3978 4067 844
Size: 2454 349 331 89 3489
Fragments arranged by size:
3489 2454 349 331 89
BsrBI CCG'CTC
Cuts at: 1394 1823 3882 4599 1394
Size: 429 2059 717 3507
Fragments arranged by size:
3507 2059 717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2263 3646 5207 5381 65
Size: 1183 1015 1383 1561 174 1396
Fragments arranged by size:
1561 1396 1383 1183 1015 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3397 3819 5356 5543 1700
Size: 110 1587 422 1537 187 2869
Fragments arranged by size:
2869 1587 1537 422 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6712
BssHII G'CGCG_C
Cuts at: 1925 2145 2152 2194 2196 1925
Size: 220 7 42 2 6441
Fragments arranged by size:
6441 220 42 7 2
BssSI C'ACGA_G
Cuts at: 2177 2201 2275 4772 5784 2177
Size: 24 74 2497 1012 3105
Fragments arranged by size:
3105 2497 1012 74 24
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6712
BstBI TT'CG_AA
Cuts at: 5516 5516
Size: 6712
BstEII G'GTnAC_C
Cuts at: 1184 3029 1184
Size: 1845 4867
BstYI r'GATC_y
Cuts at: 1972 4042 4409 4794 4811 6263 6349 6361 6707
Size: 2070 367 385 17 1452 86 12 346
Cuts at: 6707 1972
Size: 1977
Fragments arranged by size:
2070 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3124 3124
Size: 6712
BtgI C'CryG_G
Cuts at: 513 1065 2308 3625 513
Size: 552 1243 1317 3600
Fragments arranged by size:
3600 1317 1243 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5973
Fragments arranged by size:
5973 683 56
BtsI GCAGTG_nn'
Cuts at: 4228 5022 5042 4228
Size: 794 20 5898
Fragments arranged by size:
5898 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 4016 4016
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5719 809
Size: 507 4403 1802
Fragments arranged by size:
4403 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2173 2186 3483
Size: 54 1425 50 275 18 343 13 1297
Cuts at: 3483 3628 4159 5048 6677 8
Size: 145 531 889 1629 43
Fragments arranged by size:
1629 1425 1297 889 531 343 275 145
54 50 43 18 13
EagI C'GGCC_G
Cuts at: 1537 1812 2186 4159 1537
Size: 275 374 1973 4090
Fragments arranged by size:
4090 1973 374 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2303 3070 3286 4642 1353
Size: 950 767 216 1356 3423
Fragments arranged by size:
3423 1356 950 767 216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 3866 5299 5673 5819 387
Size: 1064 138 462 1815 1433 374 146 1280
Fragments arranged by size:
1815 1433 1280 1064 462 374 146 138
EcoICRI GAG'CTC
Cuts at: 3835 3835
Size: 6712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4775 5372 6159 6427 1239
Size: 393 3143 597 787 268 1524
Fragments arranged by size:
3143 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2465 2754 3159 3390 3429 3519 2465
Size: 289 405 231 39 90 5658
Fragments arranged by size:
5658 405 289 231 90 39
EcoRI G'AATT_C
Cuts at: 4021 4399 4021
Size: 378 6334
FspI TGC'GCA
Cuts at: 2291 5218 2291
Size: 2927 3785
HaeII r_GCGC'y
Cuts at: 1678 2308 3054 4006 5859 1678
Size: 630 746 952 1853 2531
Fragments arranged by size:
2531 1853 952 746 630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2304 2336 5353 5385 5427
Size: 32 135 32 732 32 3017 32 42
Cuts at: 5427 5459 1373
Size: 32 2626
Fragments arranged by size:
3017 2626 732 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2029 4056 4302 669
Size: 988 281 91 2027 246 3079
Fragments arranged by size:
3079 2027 988 281 246 91
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6712
HpaI GTT'AAC
Cuts at: 4302 4302
Size: 6712
KasI G'GCGC_C
Cuts at: 3050 3050
Size: 6712
KpnI G_GTAC'C
Cuts at: 4037 4037
Size: 6712
MfeI C'AATT_G
Cuts at: 4311 4311
Size: 6712
MluI A'CGCG_T
Cuts at: 2314 2346 6588 2314
Size: 32 4242 2438
Fragments arranged by size:
4242 2438 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3840 5826 6010 1305
Size: 2535 1986 184 2007
Fragments arranged by size:
2535 2007 1986 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4890
Fragments arranged by size:
4890 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2432 4670 5029 5188 518
Size: 648 921 345 2238 359 159 2042
Fragments arranged by size:
2238 2042 921 648 359 345 159
MspA1I CmG'CkG
Cuts at: 1606 2310 2869 2972 3086 3320 3415 3627 4128
Size: 704 559 103 114 234 95 212 501
Cuts at: 4128 4805 5953 6198 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 704 677 559 501 245 234
212 114 103 95
NarI GG'CG_CC
Cuts at: 3051 3051
Size: 6712
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6160
NdeI CA'TA_TG
Cuts at: 387 2917 3253 387
Size: 2530 336 3846
Fragments arranged by size:
3846 2530 336
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6712
NotI GC'GGCC_GC
Cuts at: 4159 4159
Size: 6712
NruI TCG'CGA
Cuts at: 2640 2640
Size: 6712
NspI r_CATG'y
Cuts at: 1132 1229 2131 2274 4070 5615 1132
Size: 97 902 143 1796 1545 2229
Fragments arranged by size:
2229 1796 1545 902 143 97
PasI CC'CwG_GG
Cuts at: 1348 3106 3691 1348
Size: 1758 585 4369
Fragments arranged by size:
4369 1758 585
PciI A'CATG_T
Cuts at: 5611 5611
Size: 6712
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2513 2921 3377 1108
Size: 1405 408 456 4443
Fragments arranged by size:
4443 1405 456 408
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 6294 6326 4781
Size: 32 1481 32 5167
Fragments arranged by size:
5167 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6712
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PspOMI G'GGCC_C
Cuts at: 2718 2754 3159 3390 3429 3519 2718
Size: 36 405 231 39 90 5911
Fragments arranged by size:
5911 405 231 90 39 36
PstI C_TGCA'G
Cuts at: 830 2899 2977 3172 3673 4064 830
Size: 2069 78 195 501 391 3478
Fragments arranged by size:
3478 2069 501 391 195 78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
PvuII CAG'CTG
Cuts at: 2869 2972 3086 3320 3415 2869
Size: 103 114 234 95 6166
Fragments arranged by size:
6166 234 114 103 95
SacI G_AGCT'C
Cuts at: 3837 3837
Size: 6712
SacII CC_GC'GG
Cuts at: 2311 3628 2311
Size: 1317 5395
SalI G'TCGA_C
Cuts at: 1936 2027 4054 1936
Size: 91 2027 4594
Fragments arranged by size:
4594 2027 91
SbfI CC_TGCA'GG
Cuts at: 4064 4064
Size: 6712
ScaI AGT'ACT
Cuts at: 4960 6672 4960
Size: 1712 5000
SfcI C'TryA_G
Cuts at: 826 1045 2749 2895 2973 3168 3669 4060 5195
Size: 219 1704 146 78 195 501 391 1135
Cuts at: 5195 5876 6067 826
Size: 681 191 1471
Fragments arranged by size:
1704 1471 1135 681 501 391 219 195
191 146 78
SfoI GGC'GCC
Cuts at: 3052 3052
Size: 6712
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6712
SmaI CCC'GGG
Cuts at: 1872 3758 4039 1872
Size: 1886 281 4545
Fragments arranged by size:
4545 1886 281
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4816 5717 5979 820
Size: 197 926 2873 901 262 1553
Fragments arranged by size:
2873 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4503 493
Size: 4010 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 2274 4070 1132
Size: 1142 1796 3774
Fragments arranged by size:
3774 1796 1142
SspI AAT'ATT
Cuts at: 5 52 4636 5
Size: 47 4584 2081
Fragments arranged by size:
4584 2081 47
StuI AGG'CCT
Cuts at: 1517 2227 1517
Size: 710 6002
StyI C'CwwG_G
Cuts at: 513 1065 2222 2448 2845 4151 513
Size: 552 1157 226 397 1306 3074
Fragments arranged by size:
3074 1306 1157 552 397 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2765 3119 4739 4756 4909 5094 5539
Size: 1255 1185 354 1620 17 153 185 445
Cuts at: 5539 325
Size: 1498
Fragments arranged by size:
1620 1498 1255 1185 445 354 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4958 6670 96
Size: 274 80 33 51 1443 2981 1712 138
Fragments arranged by size:
2981 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6672 6704 6672
Size: 32 6680
Tth111I GACn'n_nGTC
Cuts at: 1316 3892 1316
Size: 2576 4136
XbaI T'CTAG_A
Cuts at: 4048 4048
Size: 6712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2573 3370 2573
Size: 797 5915
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6712
XmaI C'CCGG_G
Cuts at: 1870 3756 4037 1870
Size: 1886 281 4545
Fragments arranged by size:
4545 1886 281
XmnI GAAnn'nnTTC
Cuts at: 2012 2111 2246 4841 2012
Size: 99 135 2595 3883
Fragments arranged by size:
3883 2595 135 99
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4520 276
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NruI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI
SrfI SwaI