(Linear) (Six-base) MAPSORT of: KIEE2048.seq Check: 195 from: 1 to: 2007 KIEE2048.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AccI GT'mk_AC Cuts at: 0 23 2007 Size: 23 1984 AflIII A'CryG_T Cuts at: 0 309 341 2007 Size: 309 32 1666 Fragments arranged by size: 1666 309 32 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 664 2007 Size: 664 1343 AlwNI CAG_nnn'CTG Cuts at: 0 282 508 1318 2007 Size: 282 226 810 689 Fragments arranged by size: 810 689 282 226 ApaI G_GGCC'C Cuts at: 0 717 753 1158 1389 1428 1518 2007 Size: 717 36 405 231 39 90 489 Fragments arranged by size: 717 489 405 231 90 39 36 ApaLI G'TGCA_C Cuts at: 0 118 2007 Size: 118 1889 AvaI C'yCGr_G Cuts at: 0 665 1751 2007 Size: 665 1086 256 Fragments arranged by size: 1086 665 256 BanI G'GyrC_C Cuts at: 0 846 1045 1495 2007 Size: 846 199 450 512 Fragments arranged by size: 846 512 450 199 BanII G_rGCy'C Cuts at: 0 717 753 924 999 1137 1158 1191 1389 Size: 717 36 171 75 138 21 33 198 Cuts at: 1389 1428 1464 1518 1832 1951 2007 Size: 39 36 54 314 119 56 Fragments arranged by size: 717 314 198 171 138 119 75 56 54 39 36 36 33 21 BbeI G_GCGC'C Cuts at: 0 1049 2007 Size: 1049 958 BbvCI CC'TCA_GC Cuts at: 0 930 2007 Size: 930 1077 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 276 310 959 993 1925 1959 2007 Size: 276 34 649 34 932 34 48 Fragments arranged by size: 932 649 276 48 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 27 507 2007 Size: 27 480 1500 Fragments arranged by size: 1500 480 27 BglI GCCn_nnn'nGGC Cuts at: 0 748 1297 1448 1513 1534 1570 1631 2007 Size: 748 549 151 65 21 36 61 376 Fragments arranged by size: 748 549 376 151 65 61 36 21 BlpI GC'TnA_GC Cuts at: 0 824 2007 Size: 824 1183 Bme1580I G_kGCm'C Cuts at: 0 122 717 753 849 1158 1389 1428 1518 Size: 122 595 36 96 309 231 39 90 Cuts at: 1518 2007 Size: 489 Fragments arranged by size: 595 489 309 231 122 96 90 39 36 BmgBI CAC'GTC Cuts at: 0 327 2007 Size: 327 1680 BmrI ACTGGGnnnn_n' Cuts at: 0 742 876 1152 1230 2007 Size: 742 134 276 78 777 Fragments arranged by size: 777 742 276 134 78 Bpu10I CC'TnA_GC Cuts at: 0 930 998 1136 2007 Size: 930 68 138 871 Fragments arranged by size: 930 871 138 68 BsaHI Gr'CG_yC Cuts at: 0 1046 2007 Size: 1046 961 BsaWI w'CCGG_w Cuts at: 0 42 2007 Size: 42 1965 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 61 674 1161 2007 Size: 61 613 487 846 Fragments arranged by size: 846 613 487 61 BseYI C'CCAG_C Cuts at: 0 508 695 716 752 923 1211 1388 1406 Size: 508 187 21 36 171 288 177 18 Cuts at: 1406 1589 2007 Size: 183 418 Fragments arranged by size: 508 418 288 187 183 177 171 36 21 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 1097 1238 1420 2007 Size: 148 949 141 182 587 Fragments arranged by size: 949 587 182 148 141 BsiEI CG_ry'CG Cuts at: 0 184 639 1815 2007 Size: 184 455 1176 192 Fragments arranged by size: 1176 455 192 184 BsiHKAI G_wGCw'C Cuts at: 0 122 1832 2007 Size: 122 1710 175 Fragments arranged by size: 1710 175 122 Bsp1286I G_dGCh'C Cuts at: 0 122 717 753 849 924 999 1137 1158 Size: 122 595 36 96 75 75 138 21 Cuts at: 1158 1191 1389 1428 1464 1518 1832 1951 2007 Size: 33 198 39 36 54 314 119 56 Fragments arranged by size: 595 314 198 138 122 119 96 75 75 56 54 39 36 36 33 21 BspEI T'CCGG_A Cuts at: 0 42 2007 Size: 42 1965 BspHI T'CATG_A Cuts at: 0 83 98 2007 Size: 83 15 1909 Fragments arranged by size: 1909 83 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1293 1642 1973 2007 Size: 1293 349 331 34 Fragments arranged by size: 1293 349 331 34 BsrBI CCG'CTC Cuts at: 0 1877 2007 Size: 1877 130 BsrDI GCAATG_nn' Cuts at: 0 258 1641 2007 Size: 258 1383 366 Fragments arranged by size: 1383 366 258 BsrFI r'CCGG_y Cuts at: 0 1392 1814 2007 Size: 1392 422 193 Fragments arranged by size: 1392 422 193 BssHII G'CGCG_C Cuts at: 0 140 147 189 191 2007 Size: 140 7 42 2 1816 Fragments arranged by size: 1816 140 42 7 2 BssSI C'ACGA_G Cuts at: 0 172 196 270 2007 Size: 172 24 74 1737 Fragments arranged by size: 1737 172 74 24 BstEII G'GTnAC_C Cuts at: 0 1024 2007 Size: 1024 983 Bsu36I CC'TnA_GG Cuts at: 0 1119 2007 Size: 1119 888 BtgI C'CryG_G Cuts at: 0 303 1620 2007 Size: 303 1317 387 Fragments arranged by size: 1317 387 303 EaeI y'GGCC_r Cuts at: 0 168 181 1478 1623 2007 Size: 168 13 1297 145 384 Fragments arranged by size: 1297 384 168 145 13 EagI C'GGCC_G Cuts at: 0 181 2007 Size: 181 1826 EarI CTCTTCn'nnn_ Cuts at: 0 298 1065 1281 2007 Size: 298 767 216 726 Fragments arranged by size: 767 726 298 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 46 1861 2007 Size: 46 1815 146 Fragments arranged by size: 1815 146 46 EcoICRI GAG'CTC Cuts at: 0 1830 2007 Size: 1830 177 EcoO109I rG'GnC_Cy Cuts at: 0 460 749 1154 1385 1424 1514 2007 Size: 460 289 405 231 39 90 493 Fragments arranged by size: 493 460 405 289 231 90 39 FspI TGC'GCA Cuts at: 0 286 2007 Size: 286 1721 HaeII r_GCGC'y Cuts at: 0 303 1049 2001 2007 Size: 303 746 952 6 Fragments arranged by size: 952 746 303 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 299 331 2007 Size: 299 32 1676 Fragments arranged by size: 1676 299 32 HincII GTy'rAC Cuts at: 0 24 2007 Size: 24 1983 KasI G'GCGC_C Cuts at: 0 1045 2007 Size: 1045 962 MluI A'CGCG_T Cuts at: 0 309 341 2007 Size: 309 32 1666 Fragments arranged by size: 1666 309 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1835 2007 Size: 1835 172 MslI CAynn'nnrTG Cuts at: 0 82 427 2007 Size: 82 345 1580 Fragments arranged by size: 1580 345 82 MspA1I CmG'CkG Cuts at: 0 305 864 967 1081 1315 1410 1622 2007 Size: 305 559 103 114 234 95 212 385 Fragments arranged by size: 559 385 305 234 212 114 103 95 NarI GG'CG_CC Cuts at: 0 1046 2007 Size: 1046 961 NdeI CA'TA_TG Cuts at: 0 912 1248 2007 Size: 912 336 759 Fragments arranged by size: 912 759 336 NruI TCG'CGA Cuts at: 0 635 2007 Size: 635 1372 NspI r_CATG'y Cuts at: 0 126 269 2007 Size: 126 143 1738 Fragments arranged by size: 1738 143 126 PasI CC'CwG_GG Cuts at: 0 1101 1686 2007 Size: 1101 585 321 Fragments arranged by size: 1101 585 321 PflMI CCAn_nnn'nTGG Cuts at: 0 508 916 1372 2007 Size: 508 408 456 635 Fragments arranged by size: 635 508 456 408 PspOMI G'GGCC_C Cuts at: 0 713 749 1154 1385 1424 1514 2007 Size: 713 36 405 231 39 90 493 Fragments arranged by size: 713 493 405 231 90 39 36 PstI C_TGCA'G Cuts at: 0 894 972 1167 1668 2007 Size: 894 78 195 501 339 Fragments arranged by size: 894 501 339 195 78 PvuII CAG'CTG Cuts at: 0 864 967 1081 1315 1410 2007 Size: 864 103 114 234 95 597 Fragments arranged by size: 864 597 234 114 103 95 SacI G_AGCT'C Cuts at: 0 1832 2007 Size: 1832 175 SacII CC_GC'GG Cuts at: 0 306 1623 2007 Size: 306 1317 384 Fragments arranged by size: 1317 384 306 SalI G'TCGA_C Cuts at: 0 22 2007 Size: 22 1985 SfcI C'TryA_G Cuts at: 0 744 890 968 1163 1664 2007 Size: 744 146 78 195 501 343 Fragments arranged by size: 744 501 343 195 146 78 SfoI GGC'GCC Cuts at: 0 1047 2007 Size: 1047 960 SmaI CCC'GGG Cuts at: 0 1753 2007 Size: 1753 254 SphI G_CATG'C Cuts at: 0 269 2007 Size: 269 1738 StuI AGG'CCT Cuts at: 0 222 2007 Size: 222 1785 StyI C'CwwG_G Cuts at: 0 217 443 840 2007 Size: 217 226 397 1167 Fragments arranged by size: 1167 397 226 217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 760 1114 2007 Size: 760 354 893 Fragments arranged by size: 893 760 354 Tth111I GACn'n_nGTC Cuts at: 0 1887 2007 Size: 1887 120 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 568 1365 2007 Size: 568 797 642 Fragments arranged by size: 797 642 568 XmaI C'CCGG_G Cuts at: 0 1751 2007 Size: 1751 256 XmnI GAAnn'nnTTC Cuts at: 0 7 106 241 2007 Size: 7 99 135 1766 Fragments arranged by size: 1766 135 99 7 Enzymes that do cut: AccI AflIII AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI Bpu10I BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstEII Bsu36I BtgI EaeI EagI EarI EciI EcoICRI EcoO109I FspI HaeII Hin4I HincII KasI MluI MmeI MslI MspA1I NarI NdeI NruI NspI PasI PflMI PspOMI PstI PvuII SacI SacII SalI SfcI SfoI SmaI SphI StuI StyI TaqII Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BclI BfrBI BglII BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BsmBI BsrGI BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MscI NaeI NcoI NgoMIV NheI NotI NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI TstI XbaI XhoI ZraI