(Circular) (Six-base) MAPSORT of: pFN21AB7277.seq Check: 9744 from: 1 to: 8569 pFN21AB7277 8569 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3801 6379 278 Size: 53 83 186 936 2265 2578 2468 Fragments arranged by size: 2578 2468 2265 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2148 5912 1937 Size: 211 3764 4594 Fragments arranged by size: 4594 3764 211 Acc65I G'GTAC_C Cuts at: 3420 5890 3420 Size: 2470 6099 AclI AA'CG_TT Cuts at: 1563 6696 7069 1563 Size: 5133 373 3063 Fragments arranged by size: 5133 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2404 3082 3448 4247 4333 4791 6632 8016 Size: 772 678 366 799 86 458 1841 1384 Cuts at: 8016 8284 1632 Size: 268 1917 Fragments arranged by size: 1917 1841 1384 799 772 678 458 366 268 86 AfeI AGC'GCT Cuts at: 3761 4886 5046 3761 Size: 1125 160 7284 Fragments arranged by size: 7284 1125 160 AflII C'TTAA_G Cuts at: 820 1017 4157 820 Size: 197 3140 5232 Fragments arranged by size: 5232 3140 197 AflIII A'CryG_T Cuts at: 1203 3090 7468 8445 1203 Size: 1887 4378 977 1327 Fragments arranged by size: 4378 1887 1327 977 AgeI A'CCGG_T Cuts at: 7400 7400 Size: 8569 AhdI GACnn_n'nnGTC Cuts at: 4501 7298 4501 Size: 2797 5772 AleI CACnn'nnGTG Cuts at: 1166 2403 3371 4016 1166 Size: 1237 968 645 5719 Fragments arranged by size: 5719 1237 968 645 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8569 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4043 4075 4043 Size: 32 8537 AlwNI CAG_nnn'CTG Cuts at: 2052 2479 2779 4072 4270 4702 5038 5179 5522 Size: 427 300 1293 198 432 336 141 343 Cuts at: 5522 5572 5596 5665 5686 6312 7884 2052 Size: 50 24 69 21 626 1572 2737 Fragments arranged by size: 2737 1572 1293 626 432 427 343 336 300 198 141 69 50 24 21 ApaI G_GGCC'C Cuts at: 4472 5092 5209 5480 4472 Size: 620 117 271 7561 Fragments arranged by size: 7561 620 271 117 ApaLI G'TGCA_C Cuts at: 4307 6626 7782 4307 Size: 2319 1156 5094 Fragments arranged by size: 5094 2319 1156 ApoI r'AATT_y Cuts at: 1493 5878 6104 6256 8365 1493 Size: 4385 226 152 2109 1697 Fragments arranged by size: 4385 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7123 160 Size: 872 6091 1606 Fragments arranged by size: 6091 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8569 AvaI C'yCGr_G Cuts at: 1870 1943 2107 2160 2174 2180 2187 3103 3626 Size: 73 164 53 14 6 7 916 523 Cuts at: 3626 4559 4925 5132 5468 5763 5894 1870 Size: 933 366 207 336 295 131 4545 Fragments arranged by size: 4545 933 916 523 366 336 295 207 164 131 73 53 14 7 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5546 5579 5546 Size: 33 8536 BamHI G'GATC_C Cuts at: 5843 5899 6266 5843 Size: 56 367 8146 Fragments arranged by size: 8146 367 56 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2101 3049 3123 3420 3883 Size: 325 217 627 314 948 74 297 463 Cuts at: 3883 4379 4852 4904 4921 5702 5890 7345 618 Size: 496 473 52 17 781 188 1455 1842 Fragments arranged by size: 1842 1455 948 781 627 496 473 463 325 314 297 217 188 74 52 17 BanII G_rGCy'C Cuts at: 1391 2181 2825 3044 3174 3249 3346 3483 4136 Size: 790 644 219 130 75 97 137 653 Cuts at: 4136 4472 4560 4568 5092 5209 5438 5453 5469 Size: 336 88 8 524 117 229 15 16 Cuts at: 5469 5480 5587 5888 1391 Size: 11 107 301 4072 Fragments arranged by size: 4072 790 653 644 524 336 301 229 219 137 130 117 107 97 88 75 16 15 11 8 BbeI G_GCGC'C Cuts at: 2105 3053 3887 2105 Size: 948 834 6787 Fragments arranged by size: 6787 948 834 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7598 BbvCI CC'TCA_GC Cuts at: 5201 5201 Size: 8569 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4916 4931 4950 4965 6760 6794 1903 Size: 34 2979 15 19 15 1795 34 3678 Fragments arranged by size: 3678 2979 1795 34 34 19 15 15 BciVI GTATCCnnnnn_n' Cuts at: 6461 7671 6461 Size: 1210 7359 BclI T'GATC_A Cuts at: 1548 4338 1548 Size: 2790 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4149 5015 5828 Size: 107 122 71 631 605 2477 866 813 Cuts at: 5828 7180 136 Size: 1352 1525 Fragments arranged by size: 2477 1525 1352 866 813 631 605 122 107 71 BglII A'GATC_T Cuts at: 4161 8564 4161 Size: 4403 4166 BlpI GC'TnA_GC Cuts at: 2078 2138 2819 4135 4976 5327 5501 5986 2078 Size: 60 681 1316 841 351 174 485 4661 Fragments arranged by size: 4661 1316 841 681 485 351 174 60 Bme1580I G_kGCm'C Cuts at: 1790 3126 3508 3565 3828 3977 4311 4472 4847 Size: 1336 382 57 263 149 334 161 375 Cuts at: 4847 4907 4926 5092 5140 5209 5480 6630 7786 Size: 60 19 166 48 69 271 1150 1156 Cuts at: 7786 1790 Size: 2573 Fragments arranged by size: 2573 1336 1156 1150 382 375 334 271 263 166 161 149 69 60 57 48 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8569 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3328 3641 3710 Size: 416 53 83 399 24 1911 313 69 Cuts at: 3710 4517 4819 6300 7258 442 Size: 807 302 1481 958 1753 Fragments arranged by size: 1911 1753 1481 958 807 416 399 313 302 83 69 53 24 BmtI G_CTAG'C Cuts at: 1056 4143 1056 Size: 3087 5482 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5404 5789 5404 Size: 385 8184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2321 2378 2640 2696 3507 3614 3685 3695 Size: 1082 57 262 56 811 107 71 10 Cuts at: 3695 4203 5836 7229 1239 Size: 508 1633 1393 2579 Fragments arranged by size: 2579 1633 1393 1082 811 508 262 107 71 57 56 10 Bpu10I CC'TnA_GC Cuts at: 3665 3792 5201 3665 Size: 127 1409 7033 Fragments arranged by size: 7033 1409 127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3056 4346 5530 6694 7559 7857 3056 Size: 1290 1184 1164 865 298 3768 Fragments arranged by size: 3768 1290 1184 1164 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2708 3434 3677 4412 5392 7232 882 Size: 617 1209 726 243 735 980 1840 2219 Fragments arranged by size: 2219 1840 1209 980 735 726 617 243 BsaAI yAC'GTr Cuts at: 493 1204 2152 3978 6360 493 Size: 711 948 1826 2382 2702 Fragments arranged by size: 2702 2382 1826 948 711 BsaBI GATnn'nnATC Cuts at: 8563 8563 Size: 8569 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2102 3050 3798 3884 Size: 53 83 186 936 569 948 748 86 Cuts at: 3884 4099 6376 6758 7393 275 Size: 215 2277 382 635 1451 Fragments arranged by size: 2277 1451 948 936 748 635 569 382 215 186 86 83 53 BsaWI w'CCGG_w Cuts at: 2973 3871 4957 5365 5458 7002 7400 7674 7821 Size: 898 1086 408 93 1544 398 274 147 Cuts at: 7821 8232 2973 Size: 411 3310 Fragments arranged by size: 3310 1544 1086 898 411 408 398 274 147 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3683 3713 4937 4967 5787 Size: 30 165 30 2280 30 1224 30 820 Cuts at: 5787 5817 1178 Size: 30 3930 Fragments arranged by size: 3930 2280 1224 820 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2432 3197 3908 4643 4943 4958 4976 5417 Size: 1246 765 711 735 300 15 18 441 Cuts at: 5417 5475 1186 Size: 58 4280 Fragments arranged by size: 4280 1246 765 735 711 441 300 58 18 15 BseYI C'CCAG_C Cuts at: 3119 3173 3268 3815 3964 4619 4705 4748 4871 Size: 54 95 547 149 655 86 43 123 Cuts at: 4871 5427 7772 8268 3119 Size: 556 2345 496 3420 Fragments arranged by size: 3420 2345 655 556 547 496 149 123 95 86 54 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2885 4498 4970 5045 5216 5516 5597 Size: 573 1149 1613 472 75 171 300 81 Cuts at: 5597 5690 1163 Size: 93 4042 Fragments arranged by size: 4042 1613 1149 573 472 300 171 93 81 75 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2135 2258 2264 4006 4211 4635 Size: 275 186 134 123 6 1742 205 424 Cuts at: 4635 4941 6019 6780 7808 1540 Size: 306 1078 761 1028 2301 Fragments arranged by size: 2301 1742 1078 1028 761 424 306 275 205 186 134 123 6 BsiHKAI G_wGCw'C Cuts at: 2442 3207 3249 4244 4311 4560 6630 6715 7786 Size: 765 42 995 67 249 2070 85 1071 Cuts at: 7786 2442 Size: 3225 Fragments arranged by size: 3225 2070 1071 995 765 249 85 67 42 BsmI GAATG_Cn' Cuts at: 6080 6173 6080 Size: 93 8476 BsmBI CGTCTCn'nnnn_ Cuts at: 2891 3938 4959 2891 Size: 1047 1021 6501 Fragments arranged by size: 6501 1047 1021 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2442 2825 3044 3126 3174 3207 Size: 399 391 261 383 219 82 48 33 Cuts at: 3207 3249 3346 3483 3508 3565 3828 3977 4136 Size: 42 97 137 25 57 263 149 159 Cuts at: 4136 4244 4311 4472 4560 4568 4847 4907 4926 Size: 108 67 161 88 8 279 60 19 Cuts at: 4926 5092 5140 5209 5438 5453 5469 5480 5587 Size: 166 48 69 229 15 16 11 107 Cuts at: 5587 5888 6630 6715 7786 1391 Size: 301 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 383 301 279 263 261 229 219 166 161 159 149 137 108 107 97 88 85 82 69 67 60 57 48 48 42 33 25 19 16 15 11 8 BspEI T'CCGG_A Cuts at: 4957 8232 4957 Size: 3275 5294 BspHI T'CATG_A Cuts at: 2835 6458 8188 2835 Size: 3623 1730 3216 Fragments arranged by size: 3623 3216 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2236 2478 4047 5924 844 Size: 1392 242 1569 1877 3489 Fragments arranged by size: 3489 1877 1569 1392 242 BsrBI CCG'CTC Cuts at: 1394 1823 2137 2749 3955 4048 5483 6456 1394 Size: 429 314 612 1206 93 1435 973 3507 Fragments arranged by size: 3507 1435 1206 973 612 429 314 93 BsrDI GCAATG_nn' Cuts at: 65 1248 3210 7064 7238 65 Size: 1183 1962 3854 174 1396 Fragments arranged by size: 3854 1962 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2042 2088 2130 2658 5160 7213 7400 Size: 110 232 46 42 528 2502 2053 187 Cuts at: 7400 1700 Size: 2869 Fragments arranged by size: 2869 2502 2053 528 232 187 110 46 42 BsrGI T'GTAC_A Cuts at: 96 4596 96 Size: 4500 4069 BssHII G'CGCG_C Cuts at: 1925 2038 2121 2241 1925 Size: 113 83 120 8253 Fragments arranged by size: 8253 120 113 83 BssSI C'ACGA_G Cuts at: 4012 4553 6629 7641 4012 Size: 541 2076 1012 4940 Fragments arranged by size: 4940 2076 1012 541 BstAPI GCAn_nnn'nTGC Cuts at: 794 2479 2911 3294 5038 794 Size: 1685 432 383 1744 4325 Fragments arranged by size: 4325 1744 1685 432 383 BstBI TT'CG_AA Cuts at: 7373 7373 Size: 8569 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8569 BstXI CCAn_nnnn'nTGG Cuts at: 3119 3373 4393 5216 3119 Size: 254 1020 823 6472 Fragments arranged by size: 6472 1020 823 254 BstYI r'GATC_y Cuts at: 1972 3348 4038 4161 4266 5271 5289 5843 5899 Size: 1376 690 123 105 1005 18 554 56 Cuts at: 5899 6266 6651 6668 8120 8206 8218 8564 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1376 1005 690 554 385 367 346 123 105 86 56 18 17 12 Bsu36I CC'TnA_GG Cuts at: 4848 4848 Size: 8569 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2258 2316 3879 3888 4769 Size: 552 939 66 188 58 1563 9 881 Cuts at: 4769 513 Size: 4313 Fragments arranged by size: 4313 1563 939 881 552 188 66 58 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2874 4009 487 Size: 683 56 1648 1135 5047 Fragments arranged by size: 5047 1648 1135 683 56 BtsI GCAGTG_nn' Cuts at: 3826 4700 5321 5678 6085 6879 6899 3826 Size: 874 621 357 407 794 20 5496 Fragments arranged by size: 5496 874 794 621 407 357 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8569 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8534 DraI TTT'AAA Cuts at: 5873 5873 Size: 8569 DraIII CAC_nnn'GTG Cuts at: 3503 4306 3503 Size: 803 7766 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7576 809 Size: 507 6260 1802 Fragments arranged by size: 6260 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2132 2255 Size: 54 1425 50 275 18 237 65 123 Cuts at: 2255 2261 2550 2877 2928 3073 4530 4632 4938 Size: 6 289 327 51 145 1457 102 306 Cuts at: 4938 5016 6016 6905 8534 8 Size: 78 1000 889 1629 43 Fragments arranged by size: 1629 1457 1425 1000 889 327 306 289 275 237 145 123 102 78 65 54 51 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2132 2255 2261 4632 4938 6016 1537 Size: 275 320 123 6 2371 306 1078 4090 Fragments arranged by size: 4090 2371 1078 320 306 275 123 6 EarI CTCTTCn'nnn_ Cuts at: 1353 4074 4095 5109 6499 1353 Size: 2721 21 1014 1390 3423 Fragments arranged by size: 3423 2721 1390 1014 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2048 2219 3914 4599 5066 7156 Size: 1064 138 459 171 1695 685 467 2090 Cuts at: 7156 7530 7676 387 Size: 374 146 1280 Fragments arranged by size: 2090 1695 1280 1064 685 467 459 374 171 146 138 EcoICRI GAG'CTC Cuts at: 3247 4558 3247 Size: 1311 7258 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2321 2378 2404 2640 2696 3082 3448 Size: 393 689 57 26 236 56 386 366 Cuts at: 3448 3507 3614 3685 3695 4203 4247 4333 4791 Size: 59 107 71 10 508 44 86 458 Cuts at: 4791 5836 6632 7229 8016 8284 1239 Size: 1045 796 597 787 268 1524 Fragments arranged by size: 1524 1045 796 787 689 597 508 458 393 386 366 268 236 107 86 71 59 57 56 44 26 10 EcoNI CCTnn'n_nnAGG Cuts at: 2231 2483 2675 3851 2231 Size: 252 192 1176 6949 Fragments arranged by size: 6949 1176 252 192 EcoO109I rG'GnC_Cy Cuts at: 2059 2248 2476 2593 2635 3004 4220 4468 4711 Size: 189 228 117 42 369 1216 248 243 Cuts at: 4711 5088 5205 5212 5476 5477 5569 5662 2059 Size: 377 117 7 264 1 92 93 4966 Fragments arranged by size: 4966 1216 377 369 264 248 243 228 189 117 117 93 92 42 7 1 EcoRI G'AATT_C Cuts at: 5878 6256 5878 Size: 378 8191 FspI TGC'GCA Cuts at: 2279 4022 7075 2279 Size: 1743 3053 3773 Fragments arranged by size: 3773 3053 1743 FspAI rTGC'GCAy Cuts at: 4022 4022 Size: 8569 HaeII r_GCGC'y Cuts at: 1678 2078 2105 3053 3744 3763 3887 4695 4888 Size: 400 27 948 691 19 124 808 193 Cuts at: 4888 5048 5189 5287 7716 1678 Size: 160 141 98 2429 2531 Fragments arranged by size: 2531 2429 948 808 691 400 193 160 141 124 98 27 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3333 3365 3683 3715 4487 Size: 32 135 32 1761 32 318 32 772 Cuts at: 4487 4519 4937 4969 4987 5019 7210 7242 7284 Size: 32 418 32 18 32 2191 32 42 Cuts at: 7284 7316 1373 Size: 32 2626 Fragments arranged by size: 2626 2191 1761 772 418 318 135 42 32 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 5913 6159 669 Size: 988 281 3975 246 3079 Fragments arranged by size: 3975 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8569 HpaI GTT'AAC Cuts at: 6159 6159 Size: 8569 KasI G'GCGC_C Cuts at: 2101 3049 3883 2101 Size: 948 834 6787 Fragments arranged by size: 6787 948 834 KpnI G_GTAC'C Cuts at: 3424 5894 3424 Size: 2470 6099 MfeI C'AATT_G Cuts at: 6168 6168 Size: 8569 MluI A'CGCG_T Cuts at: 8445 8445 Size: 8569 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3900 5663 7683 7867 1305 Size: 2595 1763 2020 184 2007 Fragments arranged by size: 2595 2020 2007 1763 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4532 10 Size: 54 1425 343 2700 4047 Fragments arranged by size: 4047 2700 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2403 2834 3371 4016 4526 4840 5711 Size: 648 1237 431 537 645 510 314 871 Cuts at: 5711 6527 6886 7045 518 Size: 816 359 159 2042 Fragments arranged by size: 2042 1237 871 816 648 645 537 510 431 359 314 159 MspA1I CmG'CkG Cuts at: 1606 2049 2065 2212 2260 2275 2318 2767 2794 Size: 443 16 147 48 15 43 449 27 Cuts at: 2794 3025 3200 3394 3705 3805 3890 4493 5242 Size: 231 175 194 311 100 85 603 749 Cuts at: 5242 5500 5581 5593 5985 6662 7810 8055 1606 Size: 258 81 12 392 677 1148 245 2120 Fragments arranged by size: 2120 1148 749 677 603 449 443 392 311 258 245 231 194 175 147 100 85 81 48 43 27 16 15 12 NaeI GCC'GGC Cuts at: 2044 2090 2132 2660 5162 2044 Size: 46 42 528 2502 5451 Fragments arranged by size: 5451 2502 528 46 42 NarI GG'CG_CC Cuts at: 2102 3050 3884 2102 Size: 948 834 6787 Fragments arranged by size: 6787 948 834 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7078 Fragments arranged by size: 7078 939 552 NdeI CA'TA_TG Cuts at: 387 3416 3728 387 Size: 3029 312 5228 Fragments arranged by size: 5228 3029 312 NgoMIV G'CCGG_C Cuts at: 2042 2088 2130 2658 5160 2042 Size: 46 42 528 2502 5451 Fragments arranged by size: 5451 2502 528 46 42 NheI G'CTAG_C Cuts at: 1052 4139 1052 Size: 3087 5482 NotI GC'GGCC_GC Cuts at: 2261 6016 2261 Size: 3755 4814 NspI r_CATG'y Cuts at: 1132 1229 3070 3094 4766 5927 7472 1132 Size: 97 1841 24 1672 1161 1545 2229 Fragments arranged by size: 2229 1841 1672 1545 1161 97 24 PasI CC'CwG_GG Cuts at: 1348 3565 5209 5350 1348 Size: 2217 1644 141 4567 Fragments arranged by size: 4567 2217 1644 141 PciI A'CATG_T Cuts at: 3090 7468 3090 Size: 4378 4191 PflMI CCAn_nnn'nTGG Cuts at: 1108 3580 5572 5665 1108 Size: 2472 1992 93 4012 Fragments arranged by size: 4012 2472 1992 93 PfoI T'CCnGG_A Cuts at: 2709 3111 3591 4041 5253 2709 Size: 402 480 450 1212 6025 Fragments arranged by size: 6025 1212 480 450 402 PmeI GTTT'AAAC Cuts at: 5873 5873 Size: 8569 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6638 6670 8151 8183 6638 Size: 32 1481 32 7024 Fragments arranged by size: 7024 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2248 4220 4711 2059 Size: 189 1972 491 5917 Fragments arranged by size: 5917 1972 491 189 PshAI GACnn'nnGTC Cuts at: 1613 5618 1613 Size: 4005 4564 PsiI TTA'TAA Cuts at: 6139 6139 Size: 8569 PspOMI G'GGCC_C Cuts at: 4468 5088 5205 5476 4468 Size: 620 117 271 7561 Fragments arranged by size: 7561 620 271 117 PspXI vC'TCGA_Gb Cuts at: 2180 3626 2180 Size: 1446 7123 PstI C_TGCA'G Cuts at: 830 2475 2793 2907 3388 4275 4440 4725 5921 Size: 1645 318 114 481 887 165 285 1196 Cuts at: 5921 830 Size: 3478 Fragments arranged by size: 3478 1645 1196 887 481 318 285 165 114 PvuI CG_AT'CG Cuts at: 2001 4006 2001 Size: 2005 6564 PvuII CAG'CTG Cuts at: 2049 2275 2767 3200 3394 3705 3805 4493 5500 Size: 226 492 433 194 311 100 688 1007 Cuts at: 5500 2049 Size: 5118 Fragments arranged by size: 5118 1007 688 492 433 311 226 194 100 RsrII CG'GwC_CG Cuts at: 2025 2025 Size: 8569 SacI G_AGCT'C Cuts at: 3249 4560 3249 Size: 1311 7258 SacII CC_GC'GG Cuts at: 2261 2319 3891 2261 Size: 58 1572 6939 Fragments arranged by size: 6939 1572 58 SalI G'TCGA_C Cuts at: 1936 5911 1936 Size: 3975 4594 SanDI GG'GwC_CC Cuts at: 4220 4711 4220 Size: 491 8078 SapI GCTCTTCn'nnn_ Cuts at: 4095 5109 4095 Size: 1014 7555 SbfI CC_TGCA'GG Cuts at: 2475 4275 5921 2475 Size: 1800 1646 5123 Fragments arranged by size: 5123 1800 1646 ScaI AGT'ACT Cuts at: 6817 8529 6817 Size: 1712 6857 SexAI A'CCwGG_T Cuts at: 3423 3423 Size: 8569 SfcI C'TryA_G Cuts at: 826 1045 2471 2789 2903 3384 3530 4271 4436 Size: 219 1426 318 114 481 146 741 165 Cuts at: 4436 4721 5588 5678 5917 7052 7733 7924 826 Size: 285 867 90 239 1135 681 191 1471 Fragments arranged by size: 1471 1426 1135 867 741 681 481 318 285 239 219 191 165 146 114 90 SfiI GGCCn_nnn'nGGCC Cuts at: 5015 5015 Size: 8569 SfoI GGC'GCC Cuts at: 2103 3051 3885 2103 Size: 948 834 6787 Fragments arranged by size: 6787 948 834 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8569 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8569 SmaI CCC'GGG Cuts at: 1872 2109 2162 2176 5896 1872 Size: 237 53 14 3720 4545 Fragments arranged by size: 4545 3720 237 53 14 SmlI C'TyrA_G Cuts at: 820 1017 1943 2180 3035 3626 4157 4361 5509 Size: 197 926 237 855 591 531 204 1148 Cuts at: 5509 5763 6673 7574 7836 820 Size: 254 910 901 262 1553 Fragments arranged by size: 1553 1148 926 910 901 855 591 531 262 254 237 204 197 SnaBI TAC'GTA Cuts at: 493 6360 493 Size: 5867 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8569 SphI G_CATG'C Cuts at: 1132 5927 1132 Size: 4795 3774 SrfI GCCC'GGGC Cuts at: 2176 2176 Size: 8569 SspI AAT'ATT Cuts at: 5 52 6493 5 Size: 47 6441 2081 Fragments arranged by size: 6441 2081 47 StuI AGG'CCT Cuts at: 1517 2583 2909 2986 3137 3857 1517 Size: 1066 326 77 151 720 6229 Fragments arranged by size: 6229 1066 720 326 151 77 StyI C'CwwG_G Cuts at: 513 1065 2004 2401 5215 6008 513 Size: 552 939 397 2814 793 3074 Fragments arranged by size: 3074 2814 939 793 552 397 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2645 2701 3383 3850 4838 4881 4885 Size: 1255 1065 56 682 467 988 43 4 Cuts at: 4885 5342 6596 6613 6766 6951 7396 325 Size: 457 1254 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1254 1065 988 682 467 457 445 185 153 56 43 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3060 3936 4596 Size: 274 80 33 51 1443 1083 876 660 Cuts at: 4596 6815 8527 96 Size: 2219 1712 138 Fragments arranged by size: 2219 1712 1443 1083 876 660 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5355 5387 8529 8561 5355 Size: 32 3142 32 5363 Fragments arranged by size: 5363 3142 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8569 XbaI T'CTAG_A Cuts at: 5905 5905 Size: 8569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2887 2923 3421 4585 2887 Size: 36 498 1164 6871 Fragments arranged by size: 6871 1164 498 36 XhoI C'TCGA_G Cuts at: 1943 2180 3626 5763 1943 Size: 237 1446 2137 4749 Fragments arranged by size: 4749 2137 1446 237 XmaI C'CCGG_G Cuts at: 1870 2107 2160 2174 5894 1870 Size: 237 53 14 3720 4545 Fragments arranged by size: 4545 3720 237 53 14 XmnI GAAnn'nnTTC Cuts at: 6698 6698 Size: 8569 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3799 6377 276 Size: 53 83 186 936 2265 2578 2468 Fragments arranged by size: 2578 2468 2265 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FalI FseI NruI NsiI PacI PmlI PsrI SwaI