(Linear) (Six-base) MAPSORT of: KIBB7277.seq Check: 61 from: 1 to: 3864 KIBB7277.orf With 182 enzymes: SgfI * July 15, 2010 18:31 .. AatII G_ACGT'C Cuts at: 0 1796 3864 Size: 1796 2068 AccI GT'mk_AC Cuts at: 0 143 3864 Size: 143 3721 Acc65I G'GTAC_C Cuts at: 0 1415 3864 Size: 1415 2449 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 399 1077 1443 2242 2328 2786 3864 Size: 399 678 366 799 86 458 1078 Fragments arranged by size: 1078 799 678 458 399 366 86 AfeI AGC'GCT Cuts at: 0 1756 2881 3041 3864 Size: 1756 1125 160 823 Fragments arranged by size: 1756 1125 823 160 AflII C'TTAA_G Cuts at: 0 2152 3864 Size: 2152 1712 AflIII A'CryG_T Cuts at: 0 1085 3864 Size: 1085 2779 AhdI GACnn_n'nnGTC Cuts at: 0 2496 3864 Size: 2496 1368 AleI CACnn'nnGTG Cuts at: 0 398 1366 2011 3864 Size: 398 968 645 1853 Fragments arranged by size: 1853 968 645 398 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2038 2070 3864 Size: 2038 32 1794 Fragments arranged by size: 2038 1794 32 AlwNI CAG_nnn'CTG Cuts at: 0 47 474 774 2067 2265 2697 3033 3174 Size: 47 427 300 1293 198 432 336 141 Cuts at: 3174 3517 3567 3591 3660 3681 3864 Size: 343 50 24 69 21 183 Fragments arranged by size: 1293 432 427 343 336 300 198 183 141 69 50 47 24 21 ApaI G_GGCC'C Cuts at: 0 2467 3087 3204 3475 3864 Size: 2467 620 117 271 389 Fragments arranged by size: 2467 620 389 271 117 ApaLI G'TGCA_C Cuts at: 0 2302 3864 Size: 2302 1562 AvaI C'yCGr_G Cuts at: 0 102 155 169 175 182 1098 1621 2554 Size: 102 53 14 6 7 916 523 933 Cuts at: 2554 2920 3127 3463 3758 3864 Size: 366 207 336 295 106 Fragments arranged by size: 933 916 523 366 336 295 207 106 102 53 14 7 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3541 3574 3864 Size: 3541 33 290 Fragments arranged by size: 3541 290 33 BamHI G'GATC_C Cuts at: 0 3838 3864 Size: 3838 26 BanI G'GyrC_C Cuts at: 0 96 1044 1118 1415 1878 2374 2847 2899 Size: 96 948 74 297 463 496 473 52 Cuts at: 2899 2916 3697 3864 Size: 17 781 167 Fragments arranged by size: 948 781 496 473 463 297 167 96 74 52 17 BanII G_rGCy'C Cuts at: 0 176 820 1039 1169 1244 1341 1478 2131 Size: 176 644 219 130 75 97 137 653 Cuts at: 2131 2467 2555 2563 3087 3204 3433 3448 3464 Size: 336 88 8 524 117 229 15 16 Cuts at: 3464 3475 3582 3864 Size: 11 107 282 Fragments arranged by size: 653 644 524 336 282 229 219 176 137 130 117 107 97 88 75 16 15 11 8 BbeI G_GCGC'C Cuts at: 0 100 1048 1882 3864 Size: 100 948 834 1982 Fragments arranged by size: 1982 948 834 100 BbvCI CC'TCA_GC Cuts at: 0 3196 3864 Size: 3196 668 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2911 2926 2945 2960 3864 Size: 2911 15 19 15 904 Fragments arranged by size: 2911 904 19 15 15 BclI T'GATC_A Cuts at: 0 2333 3864 Size: 2333 1531 BglI GCCn_nnn'nGGC Cuts at: 0 2144 3010 3823 3864 Size: 2144 866 813 41 Fragments arranged by size: 2144 866 813 41 BglII A'GATC_T Cuts at: 0 2156 3864 Size: 2156 1708 BlpI GC'TnA_GC Cuts at: 0 73 133 814 2130 2971 3322 3496 3864 Size: 73 60 681 1316 841 351 174 368 Fragments arranged by size: 1316 841 681 368 351 174 73 60 Bme1580I G_kGCm'C Cuts at: 0 1121 1503 1560 1823 1972 2306 2467 2842 Size: 1121 382 57 263 149 334 161 375 Cuts at: 2842 2902 2921 3087 3135 3204 3475 3864 Size: 60 19 166 48 69 271 389 Fragments arranged by size: 1121 389 382 375 334 271 263 166 161 149 69 60 57 48 19 BmrI ACTGGGnnnn_n' Cuts at: 0 1323 1636 1705 2512 2814 3864 Size: 1323 313 69 807 302 1050 Fragments arranged by size: 1323 1050 807 313 302 69 BmtI G_CTAG'C Cuts at: 0 2138 3864 Size: 2138 1726 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3399 3784 3864 Size: 3399 385 80 Fragments arranged by size: 3399 385 80 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 373 635 691 1502 1609 1680 1690 Size: 316 57 262 56 811 107 71 10 Cuts at: 1690 2198 3831 3864 Size: 508 1633 33 Fragments arranged by size: 1633 811 508 316 262 107 71 57 56 33 10 Bpu10I CC'TnA_GC Cuts at: 0 1660 1787 3196 3864 Size: 1660 127 1409 668 Fragments arranged by size: 1660 1409 668 127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1051 2341 3525 3864 Size: 1051 1290 1184 339 Fragments arranged by size: 1290 1184 1051 339 BsaI GGTCTCn'nnnn_ Cuts at: 0 703 1429 1672 2407 3387 3864 Size: 703 726 243 735 980 477 Fragments arranged by size: 980 735 726 703 477 243 BsaAI yAC'GTr Cuts at: 0 147 1973 3864 Size: 147 1826 1891 Fragments arranged by size: 1891 1826 147 BsaHI Gr'CG_yC Cuts at: 0 97 1045 1793 1879 2094 3864 Size: 97 948 748 86 215 1770 Fragments arranged by size: 1770 948 748 215 97 86 BsaWI w'CCGG_w Cuts at: 0 968 1866 2952 3360 3453 3864 Size: 968 898 1086 408 93 411 Fragments arranged by size: 1086 968 898 411 408 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1678 1708 2932 2962 3782 3812 3864 Size: 1678 30 1224 30 820 30 52 Fragments arranged by size: 1678 1224 820 52 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 427 1192 1903 2638 2938 2953 2971 3412 Size: 427 765 711 735 300 15 18 441 Cuts at: 3412 3470 3864 Size: 58 394 Fragments arranged by size: 765 735 711 441 427 394 300 58 18 15 BseYI C'CCAG_C Cuts at: 0 1114 1168 1263 1810 1959 2614 2700 2743 Size: 1114 54 95 547 149 655 86 43 Cuts at: 2743 2866 3422 3864 Size: 123 556 442 Fragments arranged by size: 1114 655 556 547 442 149 123 95 86 54 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 880 2493 2965 3040 3211 3511 3592 3685 Size: 880 1613 472 75 171 300 81 93 Cuts at: 3685 3864 Size: 179 Fragments arranged by size: 1613 880 472 300 179 171 93 81 75 BsiEI CG_ry'CG Cuts at: 0 130 253 259 2001 2206 2630 2936 3864 Size: 130 123 6 1742 205 424 306 928 Fragments arranged by size: 1742 928 424 306 205 130 123 6 BsiHKAI G_wGCw'C Cuts at: 0 437 1202 1244 2239 2306 2555 3864 Size: 437 765 42 995 67 249 1309 Fragments arranged by size: 1309 995 765 437 249 67 42 BsmBI CGTCTCn'nnnn_ Cuts at: 0 886 1933 2954 3864 Size: 886 1047 1021 910 Fragments arranged by size: 1047 1021 910 886 Bsp1286I G_dGCh'C Cuts at: 0 176 437 820 1039 1121 1169 1202 1244 Size: 176 261 383 219 82 48 33 42 Cuts at: 1244 1341 1478 1503 1560 1823 1972 2131 2239 Size: 97 137 25 57 263 149 159 108 Cuts at: 2239 2306 2467 2555 2563 2842 2902 2921 3087 Size: 67 161 88 8 279 60 19 166 Cuts at: 3087 3135 3204 3433 3448 3464 3475 3582 3864 Size: 48 69 229 15 16 11 107 282 Fragments arranged by size: 383 282 279 263 261 229 219 176 166 161 159 149 137 108 107 97 88 82 69 67 60 57 48 48 42 33 25 19 16 15 11 8 BspEI T'CCGG_A Cuts at: 0 2952 3864 Size: 2952 912 BspHI T'CATG_A Cuts at: 0 830 3864 Size: 830 3034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 231 473 2042 3864 Size: 231 242 1569 1822 Fragments arranged by size: 1822 1569 242 231 BsrBI CCG'CTC Cuts at: 0 132 744 1950 2043 3478 3864 Size: 132 612 1206 93 1435 386 Fragments arranged by size: 1435 1206 612 386 132 93 BsrDI GCAATG_nn' Cuts at: 0 1205 3864 Size: 1205 2659 BsrFI r'CCGG_y Cuts at: 0 37 83 125 653 3155 3864 Size: 37 46 42 528 2502 709 Fragments arranged by size: 2502 709 528 46 42 37 BsrGI T'GTAC_A Cuts at: 0 2591 3864 Size: 2591 1273 BssHII G'CGCG_C Cuts at: 0 33 116 236 3864 Size: 33 83 120 3628 Fragments arranged by size: 3628 120 83 33 BssSI C'ACGA_G Cuts at: 0 2007 2548 3864 Size: 2007 541 1316 Fragments arranged by size: 2007 1316 541 BstAPI GCAn_nnn'nTGC Cuts at: 0 474 906 1289 3033 3864 Size: 474 432 383 1744 831 Fragments arranged by size: 1744 831 474 432 383 BstXI CCAn_nnnn'nTGG Cuts at: 0 1114 1368 2388 3211 3864 Size: 1114 254 1020 823 653 Fragments arranged by size: 1114 1020 823 653 254 BstYI r'GATC_y Cuts at: 0 1343 2033 2156 2261 3266 3284 3838 3864 Size: 1343 690 123 105 1005 18 554 26 Fragments arranged by size: 1343 1005 690 554 123 105 26 18 Bsu36I CC'TnA_GG Cuts at: 0 2843 3864 Size: 2843 1021 BtgI C'CryG_G Cuts at: 0 65 253 311 1874 1883 2764 3864 Size: 65 188 58 1563 9 881 1100 Fragments arranged by size: 1563 1100 881 188 65 58 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 869 2004 3864 Size: 869 1135 1860 Fragments arranged by size: 1860 1135 869 BtsI GCAGTG_nn' Cuts at: 0 1821 2695 3316 3673 3864 Size: 1821 874 621 357 191 Fragments arranged by size: 1821 874 621 357 191 DraIII CAC_nnn'GTG Cuts at: 0 1498 2301 3864 Size: 1498 803 1563 Fragments arranged by size: 1563 1498 803 EaeI y'GGCC_r Cuts at: 0 62 127 250 256 545 872 923 1068 Size: 62 65 123 6 289 327 51 145 Cuts at: 1068 2525 2627 2933 3011 3864 Size: 1457 102 306 78 853 Fragments arranged by size: 1457 853 327 306 289 145 123 102 78 65 62 51 6 EagI C'GGCC_G Cuts at: 0 127 250 256 2627 2933 3864 Size: 127 123 6 2371 306 931 Fragments arranged by size: 2371 931 306 127 123 6 EarI CTCTTCn'nnn_ Cuts at: 0 2069 2090 3104 3864 Size: 2069 21 1014 760 Fragments arranged by size: 2069 1014 760 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 43 214 1909 2594 3061 3864 Size: 43 171 1695 685 467 803 Fragments arranged by size: 1695 803 685 467 171 43 EcoICRI GAG'CTC Cuts at: 0 1242 2553 3864 Size: 1242 1311 1311 Fragments arranged by size: 1311 1311 1242 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 316 373 399 635 691 1077 1443 1502 Size: 316 57 26 236 56 386 366 59 Cuts at: 1502 1609 1680 1690 2198 2242 2328 2786 3831 Size: 107 71 10 508 44 86 458 1045 Cuts at: 3831 3864 Size: 33 Fragments arranged by size: 1045 508 458 386 366 316 236 107 86 71 59 57 56 44 33 26 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 226 478 670 1846 3864 Size: 226 252 192 1176 2018 Fragments arranged by size: 2018 1176 252 226 192 EcoO109I rG'GnC_Cy Cuts at: 0 54 243 471 588 630 999 2215 2463 Size: 54 189 228 117 42 369 1216 248 Cuts at: 2463 2706 3083 3200 3207 3471 3472 3564 3657 Size: 243 377 117 7 264 1 92 93 Cuts at: 3657 3864 Size: 207 Fragments arranged by size: 1216 377 369 264 248 243 228 207 189 117 117 93 92 54 42 7 1 FspI TGC'GCA Cuts at: 0 274 2017 3864 Size: 274 1743 1847 Fragments arranged by size: 1847 1743 274 FspAI rTGC'GCAy Cuts at: 0 2017 3864 Size: 2017 1847 HaeII r_GCGC'y Cuts at: 0 73 100 1048 1739 1758 1882 2690 2883 Size: 73 27 948 691 19 124 808 193 Cuts at: 2883 3043 3184 3282 3864 Size: 160 141 98 582 Fragments arranged by size: 948 808 691 582 193 160 141 124 98 73 27 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1328 1360 1678 1710 2482 2514 2932 2964 Size: 1328 32 318 32 772 32 418 32 Cuts at: 2964 2982 3014 3864 Size: 18 32 850 Fragments arranged by size: 1328 850 772 418 318 32 32 32 32 32 18 KasI G'GCGC_C Cuts at: 0 96 1044 1878 3864 Size: 96 948 834 1986 Fragments arranged by size: 1986 948 834 96 KpnI G_GTAC'C Cuts at: 0 1419 3864 Size: 1419 2445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1895 3658 3864 Size: 1895 1763 206 Fragments arranged by size: 1895 1763 206 MscI TGG'CCA Cuts at: 0 2527 3864 Size: 2527 1337 MslI CAynn'nnrTG Cuts at: 0 398 829 1366 2011 2521 2835 3706 3864 Size: 398 431 537 645 510 314 871 158 Fragments arranged by size: 871 645 537 510 431 398 314 158 MspA1I CmG'CkG Cuts at: 0 44 60 207 255 270 313 762 789 Size: 44 16 147 48 15 43 449 27 Cuts at: 789 1020 1195 1389 1700 1800 1885 2488 3237 Size: 231 175 194 311 100 85 603 749 Cuts at: 3237 3495 3576 3588 3864 Size: 258 81 12 276 Fragments arranged by size: 749 603 449 311 276 258 231 194 175 147 100 85 81 48 44 43 27 16 15 12 NaeI GCC'GGC Cuts at: 0 39 85 127 655 3157 3864 Size: 39 46 42 528 2502 707 Fragments arranged by size: 2502 707 528 46 42 39 NarI GG'CG_CC Cuts at: 0 97 1045 1879 3864 Size: 97 948 834 1985 Fragments arranged by size: 1985 948 834 97 NdeI CA'TA_TG Cuts at: 0 1411 1723 3864 Size: 1411 312 2141 Fragments arranged by size: 2141 1411 312 NgoMIV G'CCGG_C Cuts at: 0 37 83 125 653 3155 3864 Size: 37 46 42 528 2502 709 Fragments arranged by size: 2502 709 528 46 42 37 NheI G'CTAG_C Cuts at: 0 2134 3864 Size: 2134 1730 NotI GC'GGCC_GC Cuts at: 0 256 3864 Size: 256 3608 NspI r_CATG'y Cuts at: 0 1065 1089 2761 3864 Size: 1065 24 1672 1103 Fragments arranged by size: 1672 1103 1065 24 PasI CC'CwG_GG Cuts at: 0 1560 3204 3345 3864 Size: 1560 1644 141 519 Fragments arranged by size: 1644 1560 519 141 PciI A'CATG_T Cuts at: 0 1085 3864 Size: 1085 2779 PflMI CCAn_nnn'nTGG Cuts at: 0 1575 3567 3660 3864 Size: 1575 1992 93 204 Fragments arranged by size: 1992 1575 204 93 PfoI T'CCnGG_A Cuts at: 0 704 1106 1586 2036 3248 3864 Size: 704 402 480 450 1212 616 Fragments arranged by size: 1212 704 616 480 450 402 PpuMI rG'GwC_Cy Cuts at: 0 54 243 2215 2706 3864 Size: 54 189 1972 491 1158 Fragments arranged by size: 1972 1158 491 189 54 PshAI GACnn'nnGTC Cuts at: 0 3613 3864 Size: 3613 251 PspOMI G'GGCC_C Cuts at: 0 2463 3083 3200 3471 3864 Size: 2463 620 117 271 393 Fragments arranged by size: 2463 620 393 271 117 PspXI vC'TCGA_Gb Cuts at: 0 175 1621 3864 Size: 175 1446 2243 Fragments arranged by size: 2243 1446 175 PstI C_TGCA'G Cuts at: 0 470 788 902 1383 2270 2435 2720 3864 Size: 470 318 114 481 887 165 285 1144 Fragments arranged by size: 1144 887 481 470 318 285 165 114 PvuI CG_AT'CG Cuts at: 0 2001 3864 Size: 2001 1863 PvuII CAG'CTG Cuts at: 0 44 270 762 1195 1389 1700 1800 2488 Size: 44 226 492 433 194 311 100 688 Cuts at: 2488 3495 3864 Size: 1007 369 Fragments arranged by size: 1007 688 492 433 369 311 226 194 100 44 RsrII CG'GwC_CG Cuts at: 0 20 3864 Size: 20 3844 SacI G_AGCT'C Cuts at: 0 1244 2555 3864 Size: 1244 1311 1309 Fragments arranged by size: 1311 1309 1244 SacII CC_GC'GG Cuts at: 0 256 314 1886 3864 Size: 256 58 1572 1978 Fragments arranged by size: 1978 1572 256 58 SanDI GG'GwC_CC Cuts at: 0 2215 2706 3864 Size: 2215 491 1158 Fragments arranged by size: 2215 1158 491 SapI GCTCTTCn'nnn_ Cuts at: 0 2090 3104 3864 Size: 2090 1014 760 Fragments arranged by size: 2090 1014 760 SbfI CC_TGCA'GG Cuts at: 0 470 2270 3864 Size: 470 1800 1594 Fragments arranged by size: 1800 1594 470 SexAI A'CCwGG_T Cuts at: 0 1418 3864 Size: 1418 2446 SfcI C'TryA_G Cuts at: 0 466 784 898 1379 1525 2266 2431 2716 Size: 466 318 114 481 146 741 165 285 Cuts at: 2716 3583 3673 3864 Size: 867 90 191 Fragments arranged by size: 867 741 481 466 318 285 191 165 146 114 90 SfiI GGCCn_nnn'nGGCC Cuts at: 0 3010 3864 Size: 3010 854 SfoI GGC'GCC Cuts at: 0 98 1046 1880 3864 Size: 98 948 834 1984 Fragments arranged by size: 1984 948 834 98 SmaI CCC'GGG Cuts at: 0 104 157 171 3864 Size: 104 53 14 3693 Fragments arranged by size: 3693 104 53 14 SmlI C'TyrA_G Cuts at: 0 175 1030 1621 2152 2356 3504 3758 3864 Size: 175 855 591 531 204 1148 254 106 Fragments arranged by size: 1148 855 591 531 254 204 175 106 SrfI GCCC'GGGC Cuts at: 0 171 3864 Size: 171 3693 StuI AGG'CCT Cuts at: 0 578 904 981 1132 1852 3864 Size: 578 326 77 151 720 2012 Fragments arranged by size: 2012 720 578 326 151 77 StyI C'CwwG_G Cuts at: 0 396 3210 3864 Size: 396 2814 654 Fragments arranged by size: 2814 654 396 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 640 696 1378 1845 2833 2876 2880 3337 Size: 640 56 682 467 988 43 4 457 Cuts at: 3337 3864 Size: 527 Fragments arranged by size: 988 682 640 527 467 457 56 43 4 TatI w'GTAC_w Cuts at: 0 1055 1931 2591 3864 Size: 1055 876 660 1273 Fragments arranged by size: 1273 1055 876 660 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3350 3382 3864 Size: 3350 32 482 Fragments arranged by size: 3350 482 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 882 918 1416 2580 3864 Size: 882 36 498 1164 1284 Fragments arranged by size: 1284 1164 882 498 36 XhoI C'TCGA_G Cuts at: 0 175 1621 3758 3864 Size: 175 1446 2137 106 Fragments arranged by size: 2137 1446 175 106 XmaI C'CCGG_G Cuts at: 0 102 155 169 3864 Size: 102 53 14 3695 Fragments arranged by size: 3695 102 53 14 ZraI GAC'GTC Cuts at: 0 1794 3864 Size: 1794 2070 Enzymes that do cut: AatII AccI Acc65I AcuI AfeI AflII AflIII AhdI AleI AloI AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SanDI SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XcmI XhoI XmaI ZraI Enzymes that do not cut: AarI AclI AgeI AlfI ApoI AscI AseI AsiSI AvrII BbsI BciVI BfrBI BmgBI BsaBI BsiWI BsmI BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI HincII HindIII HpaI MfeI MluI NcoI NruI NsiI PacI PmeI PmlI PpiI PsiI PsrI SalI ScaI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XmnI