Restriction map for
pFN21AE2101
(Circular) (Six-base) MAPSORT of: pFN21AE2101.seq Check: 2310 from: 1 to: 7291
pFN21AE2101 7291 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5101 278
Size: 53 83 186 936 3565 2468
Fragments arranged by size:
3565 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2677 4634 1937
Size: 740 1957 4594
Fragments arranged by size:
4594 1957 740
Acc65I G'GTAC_C
Cuts at: 4612 4612
Size: 7291
AclI AA'CG_TT
Cuts at: 1563 5418 5791 1563
Size: 3855 373 3063
Fragments arranged by size:
3855 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2048 2463 2574 2796 3345 3568 4559 5354
Size: 416 415 111 222 549 223 991 795
Cuts at: 5354 6738 7006 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 991 795 549 416 415 268
223 222 111
AflII C'TTAA_G
Cuts at: 820 1017 2090 820
Size: 197 1073 6021
Fragments arranged by size:
6021 1073 197
AflIII A'CryG_T
Cuts at: 1203 2468 6190 7167 1203
Size: 1265 3722 977 1327
Fragments arranged by size:
3722 1327 1265 977
AgeI A'CCGG_T
Cuts at: 6122 6122
Size: 7291
AhdI GACnn_n'nnGTC
Cuts at: 6020 6020
Size: 7291
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4284 1184
Size: 3100 4191
AlwNI CAG_nnn'CTG
Cuts at: 2269 2567 2687 3505 5034 6606 2269
Size: 298 120 818 1529 1572 2954
Fragments arranged by size:
2954 1572 1529 818 298 120
ApaLI G'TGCA_C
Cuts at: 5348 6504 5348
Size: 1156 6135
ApoI r'AATT_y
Cuts at: 1493 3179 3357 3839 3929 4011 4170 4439 4600
Size: 1686 178 482 90 82 159 269 161
Cuts at: 4600 4826 4978 7087 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1686 482 269 226 178 161
159 152 90 82
AseI AT'TA_AT
Cuts at: 160 1032 2795 5845 160
Size: 872 1763 3050 1606
Fragments arranged by size:
3050 1763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
AvaI C'yCGr_G
Cuts at: 1870 1943 4122 4616 1870
Size: 73 2179 494 4545
Fragments arranged by size:
4545 2179 494 73
AvrII C'CTAG_G
Cuts at: 2831 2831
Size: 7291
BamHI G'GATC_C
Cuts at: 4621 4988 4621
Size: 367 6924
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4612 6067 618
Size: 325 217 627 2825 1455 1842
Fragments arranged by size:
2825 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4610 1391
Size: 3219 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2168 3288 3610 928
Size: 971 269 1120 322 4609
Fragments arranged by size:
4609 1120 971 322 269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5482 5516 1903
Size: 34 3545 34 3678
Fragments arranged by size:
3678 3545 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5183 6393 5183
Size: 1210 6081
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7291
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5902 136
Size: 107 122 71 631 605 4230 1525
Fragments arranged by size:
4230 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3595 7286 3595
Size: 3691 3600
BlpI GC'TnA_GC
Cuts at: 4708 4708
Size: 7291
Bme1580I G_kGCm'C
Cuts at: 1790 5352 6508 1790
Size: 3562 1156 2573
Fragments arranged by size:
3562 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7291
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5022 5980 442
Size: 416 53 83 399 24 3605 958 1753
Fragments arranged by size:
3605 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7291
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4137 4137
Size: 7291
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2488 2838 5951 1239
Size: 1249 350 3113 2579
Fragments arranged by size:
3113 2579 1249 350
Bpu10I CC'TnA_GC
Cuts at: 2824 2824
Size: 7291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2838 3887 5416 6281 6579 2838
Size: 1049 1529 865 298 3550
Fragments arranged by size:
3550 1529 1049 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5954 882
Size: 617 4455 2219
Fragments arranged by size:
4455 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2131 2675 5082 493
Size: 711 927 544 2407 2702
Fragments arranged by size:
2702 2407 927 711 544
BsaBI GATnn'nnATC
Cuts at: 7285 7285
Size: 7291
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5098 5480 6115 275
Size: 53 83 186 936 3565 382 635 1451
Fragments arranged by size:
3565 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3063 3486 5724 6122 6396 6543 6954 3063
Size: 423 2238 398 274 147 411 3400
Fragments arranged by size:
3400 2238 423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2572 2602 2842 2872 1178
Size: 30 165 30 1169 30 240 30 5597
Fragments arranged by size:
5597 1169 240 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7291
BseYI C'CCAG_C
Cuts at: 2970 4179 6494 6990 2970
Size: 1209 2315 496 3271
Fragments arranged by size:
3271 2315 1209 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6718
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4741 5502 6530 1540
Size: 275 186 2740 761 1028 2301
Fragments arranged by size:
2740 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2534 5352 5437 6508 2534
Size: 2818 85 1071 3317
Fragments arranged by size:
3317 2818 1071 85
BsmI GAATG_Cn'
Cuts at: 4239 4802 4895 4239
Size: 563 93 6635
Fragments arranged by size:
6635 563 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2534 4610 5352 5437 6508 1391
Size: 399 744 2076 742 85 1071 2174
Fragments arranged by size:
2174 2076 1071 744 742 399 85
BspEI T'CCGG_A
Cuts at: 3063 6954 3063
Size: 3891 3400
BspHI T'CATG_A
Cuts at: 4033 5180 6910 4033
Size: 1147 1730 4414
Fragments arranged by size:
4414 1730 1147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4391 4518 4646 844
Size: 3547 127 128 3489
Fragments arranged by size:
3547 3489 128 127
BsrBI CCG'CTC
Cuts at: 1394 1823 5178 1394
Size: 429 3355 3507
Fragments arranged by size:
3507 3355 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3117 3197 3238 4432 5786 5960 65
Size: 1183 1869 80 41 1194 1354 174 1396
Fragments arranged by size:
1869 1396 1354 1194 1183 174 80 41
BsrFI r'CCGG_y
Cuts at: 1700 1810 5935 6122 1700
Size: 110 4125 187 2869
Fragments arranged by size:
4125 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7291
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7291
BssSI C'ACGA_G
Cuts at: 5351 6363 5351
Size: 1012 6279
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4189 794
Size: 3395 3896
BstBI TT'CG_AA
Cuts at: 6095 6095
Size: 7291
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7291
BstXI CCAn_nnnn'nTGG
Cuts at: 2874 3210 2874
Size: 336 6955
BstYI r'GATC_y
Cuts at: 1972 2507 3403 3595 4621 4988 5373 5390 6842
Size: 535 896 192 1026 367 385 17 1452
Cuts at: 6842 6928 6940 7286 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1026 896 535 385 367 346
192 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3819 3832 513
Size: 552 2754 13 3972
Fragments arranged by size:
3972 2754 552 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6552
Fragments arranged by size:
6552 683 56
BtsI GCAGTG_nn'
Cuts at: 4807 5601 5621 4807
Size: 794 20 6477
Fragments arranged by size:
6477 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7291
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4292 4327 529
Size: 35 3728 35 3493
Fragments arranged by size:
3728 3493 35 35
DraI TTT'AAA
Cuts at: 2626 4595 2626
Size: 1969 5322
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6298 809
Size: 507 4982 1802
Fragments arranged by size:
4982 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3507 4738 5627
Size: 54 1425 50 275 18 1677 1231 889
Cuts at: 5627 7256 8
Size: 1629 43
Fragments arranged by size:
1677 1629 1425 1231 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4738 1537
Size: 275 2926 4090
Fragments arranged by size:
4090 2926 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3212 4343 5221 1353
Size: 1859 1131 878 3423
Fragments arranged by size:
3423 1859 1131 878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5878 6252 6398 387
Size: 1064 138 4289 374 146 1280
Fragments arranged by size:
4289 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2463 2488 2574 2796 2838 3345
Size: 393 416 415 25 86 222 42 507
Cuts at: 3345 3568 4559 5354 5951 6738 7006 1239
Size: 223 991 795 597 787 268 1524
Fragments arranged by size:
1524 991 795 787 597 507 416 415
393 268 223 222 86 42 25
EcoO109I rG'GnC_Cy
Cuts at: 2828 4219 2828
Size: 1391 5900
EcoRI G'AATT_C
Cuts at: 3357 4600 4978 3357
Size: 1243 378 5670
Fragments arranged by size:
5670 1243 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3898 3898
Size: 7291
FspI TGC'GCA
Cuts at: 5797 5797
Size: 7291
HaeII r_GCGC'y
Cuts at: 1678 6438 1678
Size: 4760 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2138 2170 2486
Size: 32 135 32 431 32 103 32 316
Cuts at: 2486 2518 3571 3603 3899 3931 4021 4053 4257
Size: 32 1053 32 296 32 90 32 204
Cuts at: 4257 4289 5932 5964 6006 6038 1373
Size: 32 1643 32 42 32 2626
Fragments arranged by size:
2626 1643 1053 431 316 296 204 135
103 90 42 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 3013 3253 4003 4635 4881
Size: 988 281 1048 27 240 750 632 246
Cuts at: 4881 669
Size: 3079
Fragments arranged by size:
3079 1048 988 750 632 281 246 240
27
HindIII A'AGCT_T
Cuts at: 748 4146 748
Size: 3398 3893
HpaI GTT'AAC
Cuts at: 4881 4881
Size: 7291
KpnI G_GTAC'C
Cuts at: 4616 4616
Size: 7291
MfeI C'AATT_G
Cuts at: 4890 4890
Size: 7291
MluI A'CGCG_T
Cuts at: 7167 7167
Size: 7291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3877 6405 6589 1305
Size: 2572 2528 184 2007
Fragments arranged by size:
2572 2528 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3509 10
Size: 54 1425 343 1677 3792
Fragments arranged by size:
3792 1677 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 3208 4097 5249 5608 5767 518
Size: 648 1081 961 889 1152 359 159 2042
Fragments arranged by size:
2042 1152 1081 961 889 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4707 5384 6532 6777 1606
Size: 3101 677 1148 245 2120
Fragments arranged by size:
3101 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3819 3832 513
Size: 552 2754 13 3972
Fragments arranged by size:
3972 2754 552 13
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7291
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7291
NotI GC'GGCC_GC
Cuts at: 4738 4738
Size: 7291
NspI r_CATG'y
Cuts at: 1132 1229 2472 4271 4649 6194 1132
Size: 97 1243 1799 378 1545 2229
Fragments arranged by size:
2229 1799 1545 1243 378 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7291
PciI A'CATG_T
Cuts at: 2468 6190 2468
Size: 3722 3569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7291
PfoI T'CCnGG_A
Cuts at: 3625 3625
Size: 7291
PmeI GTTT'AAAC
Cuts at: 4595 4595
Size: 7291
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5360 5392 6873 6905 5360
Size: 32 1481 32 5746
Fragments arranged by size:
5746 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4219 4219
Size: 7291
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7291
PsiI TTA'TAA
Cuts at: 4861 4861
Size: 7291
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 7259
PstI C_TGCA'G
Cuts at: 830 4368 4643 830
Size: 3538 275 3478
Fragments arranged by size:
3538 3478 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7291
SalI G'TCGA_C
Cuts at: 1936 4633 1936
Size: 2697 4594
SbfI CC_TGCA'GG
Cuts at: 4643 4643
Size: 7291
ScaI AGT'ACT
Cuts at: 5539 7251 5539
Size: 1712 5579
SexAI A'CCwGG_T
Cuts at: 2869 2869
Size: 7291
SfcI C'TryA_G
Cuts at: 826 1045 2429 3086 4364 4639 5774 6455 6646
Size: 219 1384 657 1278 275 1135 681 191
Cuts at: 6646 826
Size: 1471
Fragments arranged by size:
1471 1384 1278 1135 681 657 275 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7291
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7291
SmaI CCC'GGG
Cuts at: 1872 4618 1872
Size: 2746 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2090 2853 3902 4122 5395 6296
Size: 197 926 147 763 1049 220 1273 901
Cuts at: 6296 6558 820
Size: 262 1553
Fragments arranged by size:
1553 1273 1049 926 901 763 262 220
197 147
SnaBI TAC'GTA
Cuts at: 493 2131 5082 493
Size: 1638 2951 2702
Fragments arranged by size:
2951 2702 1638
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7291
SphI G_CATG'C
Cuts at: 1132 4649 1132
Size: 3517 3774
SspI AAT'ATT
Cuts at: 5 52 3791 3967 4060 4187 5215 5
Size: 47 3739 176 93 127 1028 2081
Fragments arranged by size:
3739 2081 1028 176 127 93 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7291
StyI C'CwwG_G
Cuts at: 513 1065 2511 2831 3819 3832 4730 513
Size: 552 1446 320 988 13 898 3074
Fragments arranged by size:
3074 1446 988 898 552 320 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2680 3604 5318 5335 5488 5673 6118
Size: 1255 1100 924 1714 17 153 185 445
Cuts at: 6118 325
Size: 1498
Fragments arranged by size:
1714 1498 1255 1100 924 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2732 3990 5537
Size: 274 80 33 51 1443 755 1258 1547
Cuts at: 5537 7249 96
Size: 1712 138
Fragments arranged by size:
1712 1547 1443 1258 755 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2173 2205 2572 2604 7251 7283 2173
Size: 32 367 32 4647 32 2181
Fragments arranged by size:
4647 2181 367 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2121 1316
Size: 805 6486
XbaI T'CTAG_A
Cuts at: 2633 4627 2633
Size: 1994 5297
XhoI C'TCGA_G
Cuts at: 1943 4122 1943
Size: 2179 5112
XmaI C'CCGG_G
Cuts at: 1870 4616 1870
Size: 2746 4545
XmnI GAAnn'nnTTC
Cuts at: 4173 5420 4173
Size: 1247 6044
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5099 276
Size: 53 83 186 936 3565 2468
Fragments arranged by size:
3565 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PvuII RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI