################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI AlwI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BlnI BpmI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BsrGI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KpnI MluI MroI MseI NdeI NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 490 GACGTC GACGT^C AcsI 532 AAATTC [GA]^AATT[CT] AflIII 1409 ACATGT A^C[GA][CT]GT Alw44I 988 GTGCAC G^TGCAC ApaLI 988 GTGCAC G^TGCAC ApoI 532 AAATTC [GA]^AATT[CT] Asp700I 126 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 1522 GGCGCC GGCGC^C BfaI 7 CTAG C^TAG BglII 683 AGATCT A^GATCT BsaAI 1777 TACGTG [CT]AC^GT[GA] BsaBI 1812 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1484 GAGACG CGTCTC(1/5) BspHI 1070 TCATGA T^CATGA BspLU11I 1409 ACATGT A^CATGT BsrDI 1719 CATTGC GCAATG(2/0) BssHII 72 GCGCGC G^CGCGC BssSI 1182 CACGAG CACGAG(-5/-1) BstAPI 1011 GCACACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 402 GGTCACC G^GT[ACGT]ACC BstPI 402 GGTCACC G^GT[ACGT]ACC BstXI 893 CCACCTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 683 AGATCT [GA]^GATC[CT] DraIII 825 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 394 GACTTCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 411 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 402 GGTCACC G^GT[ACGT]ACC EcoT22I 1123 ATGCAT ATGCA^T KasI 1522 GGCGCC G^GCGCC KspI 25 CCGCGG CCGC^GG MaeI 7 CTAG C^TAG MamI 1812 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1098 CAATTG C^AATTG MflI 683 AGATCT [GA]^GATC[CT] MunI 1098 CAATTG C^AATTG NarI 1522 GGCGCC GG^CGCC NcoI 1784 CCATGG C^CATGG NsiI 1123 ATGCAT ATGCA^T PstI 480 CTGCAG CTGCA^G RcaI 1070 TCATGA T^CATGA SacII 25 CCGCGG CCGC^GG SapI 729 GCTCTTC GCTCTTC(1/4) ScaI 1469 AGTACT AGT^ACT SexAI 569 ACCTGGT A^CC[AT]GGT SfcI 480 CTGCAG C^T[GA][CT]AG SfoI 1522 GGCGCC GGC^GCC XhoII 683 AGATCT [GA]^GATC[CT] XmnI 126 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 1195 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1297 CAGCCCCTG ApaI 12 GGGCCC GGGCC^C 98 GGGCCC AspI 178 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 364 GACATCGTC BbvCI 1291 CCTCAGC CCTCAGC(-5/-2) 1395 CCTCAGC BglI 1495 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1857 GCCGCCCTGGC BsaI 91 GAGACC GGTCTC(1/5) 1454 GGTCTC BsaWI 136 TCCGGT [AT]^CCGG[AT] 1445 TCCGGT EagI 358 CGGCCG C^GGCCG 1078 CGGCCG EclXI 358 CGGCCG C^GGCCG 1078 CGGCCG Eco52I 358 CGGCCG C^GGCCG 1078 CGGCCG EcoT14I 416 CCAAGG C^C[AT][AT]GG 1784 CCATGG HaeII 21 AGCGCC [GA]GCGC^[CT] 1522 GGCGCC HinfI 453 GACTC G^A[ACGT]TC 1117 GAGTC MslI 219 CATTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 834 CATCTCCGTG PflFI 178 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 364 GACATCGTC PleI 453 GACTC GAGTC(4/5) 1117 GAGTC PpuMI 299 GGGACCC [GA]G^G[AT]CC[CT] 1390 AGGTCCC PvuII 305 CAGCTG CAG^CTG 598 CAGCTG SmaI 95 CCCGGG CCC^GGG 1694 CCCGGG StyI 416 CCAAGG C^C[AT][AT]GG 1784 CCATGG Tsp509I 533 AATT ^AATT 1099 AATT Tth111I 178 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 364 GACATCGTC XmaCI 95 CCCGGG C^CCGGG 1694 CCCGGG XmaI 95 CCCGGG C^CCGGG 1694 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BlpI BmyI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI RsaI SacI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp45I TspRI TthHB8I XcmI