(Circular) (Six-base) MAPSORT of: pFN21AE2034.seq Check: 9003 from: 1 to: 6586 pFN21AE2034 6586 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3684 3684 Size: 6586 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2499 4396 278 Size: 53 83 186 936 963 1897 2468 Fragments arranged by size: 2468 1897 963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3929 1937 Size: 1992 4594 Acc65I G'GTAC_C Cuts at: 3907 3907 Size: 6586 AclI AA'CG_TT Cuts at: 1563 4713 5086 1563 Size: 3150 373 3063 Fragments arranged by size: 3150 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 3758 4649 6033 6301 1632 Size: 515 1611 891 1384 268 1917 Fragments arranged by size: 1917 1611 1384 891 515 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6389 AflIII A'CryG_T Cuts at: 1203 3414 5485 6462 1203 Size: 2211 2071 977 1327 Fragments arranged by size: 2211 2071 1327 977 AgeI A'CCGG_T Cuts at: 5417 5417 Size: 6586 AhdI GACnn_n'nnGTC Cuts at: 5315 5315 Size: 6586 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6586 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2861 1184 Size: 1677 4909 AlwNI CAG_nnn'CTG Cuts at: 3205 3307 4329 5901 3205 Size: 102 1022 1572 3890 Fragments arranged by size: 3890 1572 1022 102 ApaI G_GGCC'C Cuts at: 2021 2107 2021 Size: 86 6500 ApaLI G'TGCA_C Cuts at: 2993 4643 5799 2993 Size: 1650 1156 3780 Fragments arranged by size: 3780 1650 1156 ApoI r'AATT_y Cuts at: 1493 2537 3895 4121 4273 6382 1493 Size: 1044 1358 226 152 2109 1697 Fragments arranged by size: 2109 1697 1358 1044 226 152 AseI AT'TA_AT Cuts at: 160 1032 5140 160 Size: 872 4108 1606 Fragments arranged by size: 4108 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 AvaI C'yCGr_G Cuts at: 1870 1943 2100 3161 3486 3503 3628 3699 3911 Size: 73 157 1061 325 17 125 71 212 Cuts at: 3911 1870 Size: 4545 Fragments arranged by size: 4545 1061 325 212 157 125 73 71 17 BamHI G'GATC_C Cuts at: 3916 4283 3916 Size: 367 6219 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2651 3194 3365 3373 3453 Size: 325 217 627 864 543 171 8 80 Cuts at: 3453 3527 3907 5362 618 Size: 74 380 1455 1842 Fragments arranged by size: 1842 1455 864 627 543 380 325 217 171 80 74 8 BanII G_rGCy'C Cuts at: 1391 2021 2107 2355 2483 3180 3564 3615 3905 Size: 630 86 248 128 697 384 51 290 Cuts at: 3905 1391 Size: 4072 Fragments arranged by size: 4072 697 630 384 290 248 128 86 51 BbeI G_GCGC'C Cuts at: 3531 3531 Size: 6586 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2788 2857 2971 3037 3753 928 Size: 971 889 69 114 66 716 3761 Fragments arranged by size: 3761 971 889 716 114 69 66 BbvCI CC'TCA_GC Cuts at: 3297 3401 3297 Size: 104 6482 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4777 4811 1903 Size: 34 2840 34 3678 Fragments arranged by size: 3678 2840 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4478 5688 4478 Size: 1210 5376 BclI T'GATC_A Cuts at: 1548 1548 Size: 6586 BfrBI ATG'CAT Cuts at: 3130 3130 Size: 6586 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3506 3868 5197 Size: 107 122 71 631 605 1834 362 1329 Cuts at: 5197 136 Size: 1525 Fragments arranged by size: 1834 1525 1329 631 605 362 122 107 71 BglII A'GATC_T Cuts at: 2688 6581 2688 Size: 3893 2693 BlpI GC'TnA_GC Cuts at: 2217 3252 3558 4003 2217 Size: 1035 306 445 4800 Fragments arranged by size: 4800 1035 445 306 Bme1580I G_kGCm'C Cuts at: 1790 2021 2107 2654 2997 3148 4647 5803 1790 Size: 231 86 547 343 151 1499 1156 2573 Fragments arranged by size: 2573 1499 1156 547 343 231 151 86 BmgBI CAC'GTC Cuts at: 1318 2185 2230 1318 Size: 867 45 5674 Fragments arranged by size: 5674 867 45 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4317 5275 442 Size: 416 53 83 399 24 2900 958 1753 Fragments arranged by size: 2900 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6586 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5246 1239 Size: 4007 2579 Bpu10I CC'TnA_GC Cuts at: 3297 3401 3297 Size: 104 6482 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4711 5576 5874 4711 Size: 865 298 5423 Fragments arranged by size: 5423 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2090 3465 5249 882 Size: 617 591 1375 1784 2219 Fragments arranged by size: 2219 1784 1375 617 591 BsaAI yAC'GTr Cuts at: 493 1204 3784 4377 493 Size: 711 2580 593 2702 Fragments arranged by size: 2702 2580 711 593 BsaBI GATnn'nnATC Cuts at: 3821 6580 3821 Size: 2759 3827 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2496 2643 3447 3528 Size: 53 83 186 936 963 147 804 81 Cuts at: 3528 3714 4393 4775 5410 275 Size: 186 679 382 635 1451 Fragments arranged by size: 1451 963 936 804 679 635 382 186 186 147 83 81 53 BsaWI w'CCGG_w Cuts at: 2141 3450 5019 5417 5691 5838 6249 2141 Size: 1309 1569 398 274 147 411 2478 Fragments arranged by size: 2478 1569 1309 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2465 2495 2792 2822 3054 Size: 30 165 30 1062 30 297 30 232 Cuts at: 3054 3084 1178 Size: 30 4680 Fragments arranged by size: 4680 1062 297 232 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 3062 3285 1186 Size: 943 933 223 4487 Fragments arranged by size: 4487 943 933 223 BseYI C'CCAG_C Cuts at: 2301 2308 3838 5789 6285 2301 Size: 7 1530 1951 496 2602 Fragments arranged by size: 2602 1951 1530 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2471 2512 3191 1163 Size: 573 735 41 679 4558 Fragments arranged by size: 4558 735 679 573 41 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2366 3086 3802 4036 4797 5825 Size: 275 186 365 720 716 234 761 1028 Cuts at: 5825 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 720 716 365 275 234 186 BsiHKAI G_wGCw'C Cuts at: 2355 2483 2988 2997 3564 4647 4732 5803 2355 Size: 128 505 9 567 1083 85 1071 3138 Fragments arranged by size: 3138 1083 1071 567 505 128 85 9 BsmI GAATG_Cn' Cuts at: 4097 4190 4097 Size: 93 6493 BsmBI CGTCTCn'nnnn_ Cuts at: 3483 3483 Size: 6586 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2107 2355 2483 2654 2988 2997 Size: 399 231 86 248 128 171 334 9 Cuts at: 2997 3148 3180 3564 3615 3905 4647 4732 5803 Size: 151 32 384 51 290 742 85 1071 Cuts at: 5803 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 384 334 290 248 231 171 151 128 86 85 51 32 9 BspEI T'CCGG_A Cuts at: 6249 6249 Size: 6586 BspHI T'CATG_A Cuts at: 3075 4475 6205 3075 Size: 1400 1730 3456 Fragments arranged by size: 3456 1730 1400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2811 2940 3684 3941 844 Size: 1967 129 744 257 3489 Fragments arranged by size: 3489 1967 744 257 129 BsrBI CCG'CTC Cuts at: 1394 1823 2119 2734 3003 3192 4473 1394 Size: 429 296 615 269 189 1281 3507 Fragments arranged by size: 3507 1281 615 429 296 269 189 BsrDI GCAATG_nn' Cuts at: 65 1248 3723 5081 5255 65 Size: 1183 2475 1358 174 1396 Fragments arranged by size: 2475 1396 1358 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 3108 3247 3456 3524 3688 5230 Size: 110 256 1042 139 209 68 164 1542 Cuts at: 5230 5417 1700 Size: 187 2869 Fragments arranged by size: 2869 1542 1042 256 209 187 164 139 110 68 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6586 BssHII G'CGCG_C Cuts at: 1925 2077 1925 Size: 152 6434 BssSI C'ACGA_G Cuts at: 3187 4646 5658 3187 Size: 1459 1012 4115 Fragments arranged by size: 4115 1459 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3022 794 Size: 2228 4358 BstBI TT'CG_AA Cuts at: 5390 5390 Size: 6586 BstEII G'GTnAC_C Cuts at: 1184 2407 1184 Size: 1223 5363 BstXI CCAn_nnnn'nTGG Cuts at: 2905 2905 Size: 6586 BstYI r'GATC_y Cuts at: 1972 2688 3916 4283 4668 4685 6137 6223 6235 Size: 716 1228 367 385 17 1452 86 12 Cuts at: 6235 6581 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1228 716 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2030 2166 2646 3090 3361 3636 Size: 552 939 26 136 480 444 271 275 Cuts at: 3636 3789 513 Size: 153 3310 Fragments arranged by size: 3310 939 552 480 444 275 271 153 136 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2197 487 Size: 683 56 971 4876 Fragments arranged by size: 4876 971 683 56 BtsI GCAGTG_nn' Cuts at: 2459 4102 4896 4916 2459 Size: 1643 794 20 4129 Fragments arranged by size: 4129 1643 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6586 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6551 DraI TTT'AAA Cuts at: 3890 3890 Size: 6586 DraIII CAC_nnn'GTG Cuts at: 2835 2835 Size: 6586 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2405 5593 809 Size: 507 1089 3188 1802 Fragments arranged by size: 3188 1802 1089 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2169 2363 2466 Size: 54 1425 50 275 18 339 194 103 Cuts at: 2466 3083 3138 3384 3514 3786 4033 4922 6551 Size: 617 55 246 130 272 247 889 1629 Cuts at: 6551 8 Size: 43 Fragments arranged by size: 1629 1425 889 617 339 275 272 247 246 194 130 103 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2363 3083 4033 1537 Size: 275 551 720 950 4090 Fragments arranged by size: 4090 950 720 551 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2141 2741 3622 3860 4516 1353 Size: 788 600 881 238 656 3423 Fragments arranged by size: 3423 881 788 656 600 238 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5173 5547 5693 387 Size: 1064 138 3584 374 146 1280 Fragments arranged by size: 3584 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2353 2481 3562 2353 Size: 128 1081 5377 Fragments arranged by size: 5377 1081 128 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 3758 4649 5246 6033 6301 1239 Size: 393 515 1611 891 597 787 268 1524 Fragments arranged by size: 1611 1524 891 787 597 515 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2420 2420 Size: 6586 EcoO109I rG'GnC_Cy Cuts at: 2017 2018 2305 3396 2017 Size: 1 287 1091 5207 Fragments arranged by size: 5207 1091 287 1 EcoRI G'AATT_C Cuts at: 3895 4273 3895 Size: 378 6208 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 6586 FspI TGC'GCA Cuts at: 5092 5092 Size: 6586 HaeII r_GCGC'y Cuts at: 1678 2030 3531 5733 1678 Size: 352 1501 2202 2531 Fragments arranged by size: 2531 2202 1501 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3566 3598 5227 5259 5301 Size: 32 135 32 1994 32 1629 32 42 Cuts at: 5301 5333 1373 Size: 32 2626 Fragments arranged by size: 2626 1994 1629 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3930 4176 669 Size: 988 281 1992 246 3079 Fragments arranged by size: 3079 1992 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6586 HpaI GTT'AAC Cuts at: 4176 4176 Size: 6586 KasI G'GCGC_C Cuts at: 3527 3527 Size: 6586 KpnI G_GTAC'C Cuts at: 3911 3911 Size: 6586 MfeI C'AATT_G Cuts at: 3103 4185 3103 Size: 1082 5504 MluI A'CGCG_T Cuts at: 6462 6462 Size: 6586 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3306 5700 5884 1305 Size: 2001 2394 184 2007 Fragments arranged by size: 2394 2007 2001 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2171 3140 3386 3516 3788 Size: 54 1425 343 339 969 246 130 272 Cuts at: 3788 10 Size: 2808 Fragments arranged by size: 2808 1425 969 343 339 272 246 130 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 2843 4544 4903 5062 518 Size: 648 1062 615 1701 359 159 2042 Fragments arranged by size: 2042 1701 1062 648 615 359 159 MspA1I CmG'CkG Cuts at: 1606 2032 2047 2312 2605 4002 4679 5827 6072 Size: 426 15 265 293 1397 677 1148 245 Cuts at: 6072 1606 Size: 2120 Fragments arranged by size: 2120 1397 1148 677 426 293 265 245 15 NaeI GCC'GGC Cuts at: 3110 3249 3526 3690 3110 Size: 139 277 164 6006 Fragments arranged by size: 6006 277 164 139 NarI GG'CG_CC Cuts at: 3528 3528 Size: 6586 NcoI C'CATG_G Cuts at: 513 1065 2004 3789 513 Size: 552 939 1785 3310 Fragments arranged by size: 3310 1785 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6586 NgoMIV G'CCGG_C Cuts at: 3108 3247 3524 3688 3108 Size: 139 277 164 6006 Fragments arranged by size: 6006 277 164 139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6586 NotI GC'GGCC_GC Cuts at: 4033 4033 Size: 6586 NsiI A_TGCA'T Cuts at: 3132 3132 Size: 6586 NspI r_CATG'y Cuts at: 1132 1229 2587 3418 3603 3944 5489 1132 Size: 97 1358 831 185 341 1545 2229 Fragments arranged by size: 2229 1545 1358 831 341 185 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6586 PciI A'CATG_T Cuts at: 3414 5485 3414 Size: 2071 4515 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6586 PfoI T'CCnGG_A Cuts at: 2546 3048 2546 Size: 502 6084 PmeI GTTT'AAAC Cuts at: 3890 3890 Size: 6586 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4655 4687 6168 6200 4655 Size: 32 1481 32 5041 Fragments arranged by size: 5041 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 3396 2305 Size: 1091 5495 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6586 PsiI TTA'TAA Cuts at: 4156 4156 Size: 6586 PspOMI G'GGCC_C Cuts at: 2017 2103 2017 Size: 86 6500 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2921 2953 2921 Size: 32 6554 PstI C_TGCA'G Cuts at: 830 2489 3938 830 Size: 1659 1449 3478 Fragments arranged by size: 3478 1659 1449 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6586 PvuII CAG'CTG Cuts at: 2312 2605 2312 Size: 293 6293 SacI G_AGCT'C Cuts at: 2355 2483 3564 2355 Size: 128 1081 5377 Fragments arranged by size: 5377 1081 128 SacII CC_GC'GG Cuts at: 2033 2033 Size: 6586 SalI G'TCGA_C Cuts at: 1936 3928 1936 Size: 1992 4594 SanDI GG'GwC_CC Cuts at: 2305 2305 Size: 6586 SapI GCTCTTCn'nnn_ Cuts at: 2741 2741 Size: 6586 SbfI CC_TGCA'GG Cuts at: 3938 3938 Size: 6586 ScaI AGT'ACT Cuts at: 3476 4834 6546 3476 Size: 1358 1712 3516 Fragments arranged by size: 3516 1712 1358 SexAI A'CCwGG_T Cuts at: 2574 2574 Size: 6586 SfcI C'TryA_G Cuts at: 826 1045 2485 3934 5069 5750 5941 826 Size: 219 1440 1449 1135 681 191 1471 Fragments arranged by size: 1471 1449 1440 1135 681 219 191 SfoI GGC'GCC Cuts at: 3529 3529 Size: 6586 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6586 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6586 SmaI CCC'GGG Cuts at: 1872 2102 3701 3913 1872 Size: 230 1599 212 4545 Fragments arranged by size: 4545 1599 230 212 SmlI C'TyrA_G Cuts at: 820 1017 1943 4690 5591 5853 820 Size: 197 926 2747 901 262 1553 Fragments arranged by size: 2747 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4377 493 Size: 3884 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6586 SphI G_CATG'C Cuts at: 1132 3944 1132 Size: 2812 3774 SspI AAT'ATT Cuts at: 5 52 4510 5 Size: 47 4458 2081 Fragments arranged by size: 4458 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6586 StyI C'CwwG_G Cuts at: 513 1065 2004 2421 3789 4025 513 Size: 552 939 417 1368 236 3074 Fragments arranged by size: 3074 1368 939 552 417 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2257 2426 3212 3383 3563 4613 4630 Size: 1255 677 169 786 171 180 1050 17 Cuts at: 4630 4783 4968 5413 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1050 786 677 445 185 180 171 169 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3474 3720 4832 Size: 274 80 33 51 1443 1497 246 1112 Cuts at: 4832 6544 96 Size: 1712 138 Fragments arranged by size: 1712 1497 1443 1112 274 246 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2895 2927 3054 3086 6546 6578 2895 Size: 32 127 32 3460 32 2903 Fragments arranged by size: 3460 2903 127 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2186 2372 1316 Size: 870 186 5530 Fragments arranged by size: 5530 870 186 XbaI T'CTAG_A Cuts at: 3922 3922 Size: 6586 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2089 2572 3133 3338 2089 Size: 483 561 205 5337 Fragments arranged by size: 5337 561 483 205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6586 XmaI C'CCGG_G Cuts at: 1870 2100 3699 3911 1870 Size: 230 1599 212 4545 Fragments arranged by size: 4545 1599 230 212 XmnI GAAnn'nnTTC Cuts at: 2135 4715 2135 Size: 2580 4006 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2497 4394 276 Size: 53 83 186 936 963 1897 2468 Fragments arranged by size: 2468 1897 963 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI PacI PmlI PspXI RsrII SfiI SrfI SwaI