(Linear) (Six-base) MAPSORT of: KIEE2034.seq Check: 5862 from: 1 to: 1881 KIEE2034.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1679 1881 Size: 1679 202 AatII G_ACGT'C Cuts at: 0 494 1881 Size: 494 1387 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 1753 1881 Size: 142 1611 128 Fragments arranged by size: 1611 142 128 AflIII A'CryG_T Cuts at: 0 1409 1881 Size: 1409 472 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 856 1881 Size: 856 1025 AlwNI CAG_nnn'CTG Cuts at: 0 1200 1302 1881 Size: 1200 102 579 Fragments arranged by size: 1200 579 102 ApaI G_GGCC'C Cuts at: 0 16 102 1881 Size: 16 86 1779 Fragments arranged by size: 1779 86 16 ApaLI G'TGCA_C Cuts at: 0 988 1881 Size: 988 893 ApoI r'AATT_y Cuts at: 0 532 1881 Size: 532 1349 AvaI C'yCGr_G Cuts at: 0 95 1156 1481 1498 1623 1694 1881 Size: 95 1061 325 17 125 71 187 Fragments arranged by size: 1061 325 187 125 95 71 17 BanI G'GyrC_C Cuts at: 0 646 1189 1360 1368 1448 1522 1881 Size: 646 543 171 8 80 74 359 Fragments arranged by size: 646 543 359 171 80 74 8 BanII G_rGCy'C Cuts at: 0 16 102 350 478 1175 1559 1610 1881 Size: 16 86 248 128 697 384 51 271 Fragments arranged by size: 697 384 271 248 128 86 51 16 BbeI G_GCGC'C Cuts at: 0 1526 1881 Size: 1526 355 BbsI GAAGACnn'nnnn_ Cuts at: 0 783 852 966 1032 1748 1881 Size: 783 69 114 66 716 133 Fragments arranged by size: 783 716 133 114 69 66 BbvCI CC'TCA_GC Cuts at: 0 1292 1396 1881 Size: 1292 104 485 Fragments arranged by size: 1292 485 104 BfrBI ATG'CAT Cuts at: 0 1125 1881 Size: 1125 756 BglI GCCn_nnn'nGGC Cuts at: 0 1501 1863 1881 Size: 1501 362 18 Fragments arranged by size: 1501 362 18 BglII A'GATC_T Cuts at: 0 683 1881 Size: 683 1198 BlpI GC'TnA_GC Cuts at: 0 212 1247 1553 1881 Size: 212 1035 306 328 Fragments arranged by size: 1035 328 306 212 Bme1580I G_kGCm'C Cuts at: 0 16 102 649 992 1143 1881 Size: 16 86 547 343 151 738 Fragments arranged by size: 738 547 343 151 86 16 BmgBI CAC'GTC Cuts at: 0 180 225 1881 Size: 180 45 1656 Fragments arranged by size: 1656 180 45 Bpu10I CC'TnA_GC Cuts at: 0 1292 1396 1881 Size: 1292 104 485 Fragments arranged by size: 1292 485 104 BsaI GGTCTCn'nnnn_ Cuts at: 0 85 1460 1881 Size: 85 1375 421 Fragments arranged by size: 1375 421 85 BsaAI yAC'GTr Cuts at: 0 1779 1881 Size: 1779 102 BsaBI GATnn'nnATC Cuts at: 0 1816 1881 Size: 1816 65 BsaHI Gr'CG_yC Cuts at: 0 491 638 1442 1523 1709 1881 Size: 491 147 804 81 186 172 Fragments arranged by size: 804 491 186 172 147 81 BsaWI w'CCGG_w Cuts at: 0 136 1445 1881 Size: 136 1309 436 Fragments arranged by size: 1309 436 136 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 460 490 787 817 1049 1079 1881 Size: 460 30 297 30 232 30 802 Fragments arranged by size: 802 460 297 232 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 124 1057 1280 1881 Size: 124 933 223 601 Fragments arranged by size: 933 601 223 124 BseYI C'CCAG_C Cuts at: 0 296 303 1833 1881 Size: 296 7 1530 48 Fragments arranged by size: 1530 296 48 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 507 1186 1881 Size: 466 41 679 695 Fragments arranged by size: 695 679 466 41 BsiEI CG_ry'CG Cuts at: 0 361 1081 1797 1881 Size: 361 720 716 84 Fragments arranged by size: 720 716 361 84 BsiHKAI G_wGCw'C Cuts at: 0 350 478 983 992 1559 1881 Size: 350 128 505 9 567 322 Fragments arranged by size: 567 505 350 322 128 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1478 1881 Size: 1478 403 Bsp1286I G_dGCh'C Cuts at: 0 16 102 350 478 649 983 992 1143 Size: 16 86 248 128 171 334 9 151 Cuts at: 1143 1175 1559 1610 1881 Size: 32 384 51 271 Fragments arranged by size: 384 334 271 248 171 151 128 86 51 32 16 9 BspHI T'CATG_A Cuts at: 0 1070 1881 Size: 1070 811 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 806 935 1679 1881 Size: 806 129 744 202 Fragments arranged by size: 806 744 202 129 BsrBI CCG'CTC Cuts at: 0 114 729 998 1187 1881 Size: 114 615 269 189 694 Fragments arranged by size: 694 615 269 189 114 BsrDI GCAATG_nn' Cuts at: 0 1718 1881 Size: 1718 163 BsrFI r'CCGG_y Cuts at: 0 61 1103 1242 1451 1519 1683 1881 Size: 61 1042 139 209 68 164 198 Fragments arranged by size: 1042 209 198 164 139 68 61 BssHII G'CGCG_C Cuts at: 0 72 1881 Size: 72 1809 BssSI C'ACGA_G Cuts at: 0 1182 1881 Size: 1182 699 BstAPI GCAn_nnn'nTGC Cuts at: 0 1017 1881 Size: 1017 864 BstEII G'GTnAC_C Cuts at: 0 402 1881 Size: 402 1479 BstXI CCAn_nnnn'nTGG Cuts at: 0 900 1881 Size: 900 981 BstYI r'GATC_y Cuts at: 0 683 1881 Size: 683 1198 BtgI C'CryG_G Cuts at: 0 25 161 641 1085 1356 1631 1784 1881 Size: 25 136 480 444 271 275 153 97 Fragments arranged by size: 480 444 275 271 153 136 97 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 192 1881 Size: 192 1689 BtsI GCAGTG_nn' Cuts at: 0 454 1881 Size: 454 1427 DraIII CAC_nnn'GTG Cuts at: 0 830 1881 Size: 830 1051 DrdI GACnn_nn'nnGTC Cuts at: 0 400 1881 Size: 400 1481 EaeI y'GGCC_r Cuts at: 0 164 358 461 1078 1133 1379 1509 1781 Size: 164 194 103 617 55 246 130 272 Cuts at: 1781 1881 Size: 100 Fragments arranged by size: 617 272 246 194 164 130 103 100 55 EagI C'GGCC_G Cuts at: 0 358 1078 1881 Size: 358 720 803 Fragments arranged by size: 803 720 358 EarI CTCTTCn'nnn_ Cuts at: 0 136 736 1617 1855 1881 Size: 136 600 881 238 26 Fragments arranged by size: 881 600 238 136 26 EcoICRI GAG'CTC Cuts at: 0 348 476 1557 1881 Size: 348 128 1081 324 Fragments arranged by size: 1081 348 324 128 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 1753 1881 Size: 142 1611 128 Fragments arranged by size: 1611 142 128 EcoNI CCTnn'n_nnAGG Cuts at: 0 415 1881 Size: 415 1466 EcoO109I rG'GnC_Cy Cuts at: 0 12 13 300 1391 1881 Size: 12 1 287 1091 490 Fragments arranged by size: 1091 490 287 12 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 147 1881 Size: 147 1734 HaeII r_GCGC'y Cuts at: 0 25 1526 1881 Size: 25 1501 355 Fragments arranged by size: 1501 355 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1561 1593 1881 Size: 1561 32 288 Fragments arranged by size: 1561 288 32 KasI G'GCGC_C Cuts at: 0 1522 1881 Size: 1522 359 MfeI C'AATT_G Cuts at: 0 1098 1881 Size: 1098 783 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1301 1881 Size: 1301 580 MscI TGG'CCA Cuts at: 0 166 1135 1381 1511 1783 1881 Size: 166 969 246 130 272 98 Fragments arranged by size: 969 272 246 166 130 98 MslI CAynn'nnrTG Cuts at: 0 223 838 1881 Size: 223 615 1043 Fragments arranged by size: 1043 615 223 MspA1I CmG'CkG Cuts at: 0 27 42 307 600 1881 Size: 27 15 265 293 1281 Fragments arranged by size: 1281 293 265 27 15 NaeI GCC'GGC Cuts at: 0 1105 1244 1521 1685 1881 Size: 1105 139 277 164 196 Fragments arranged by size: 1105 277 196 164 139 NarI GG'CG_CC Cuts at: 0 1523 1881 Size: 1523 358 NcoI C'CATG_G Cuts at: 0 1784 1881 Size: 1784 97 NgoMIV G'CCGG_C Cuts at: 0 1103 1242 1519 1683 1881 Size: 1103 139 277 164 198 Fragments arranged by size: 1103 277 198 164 139 NsiI A_TGCA'T Cuts at: 0 1127 1881 Size: 1127 754 NspI r_CATG'y Cuts at: 0 582 1413 1598 1881 Size: 582 831 185 283 Fragments arranged by size: 831 582 283 185 PciI A'CATG_T Cuts at: 0 1409 1881 Size: 1409 472 PfoI T'CCnGG_A Cuts at: 0 541 1043 1881 Size: 541 502 838 Fragments arranged by size: 838 541 502 PpuMI rG'GwC_Cy Cuts at: 0 300 1391 1881 Size: 300 1091 490 Fragments arranged by size: 1091 490 300 PspOMI G'GGCC_C Cuts at: 0 12 98 1881 Size: 12 86 1783 Fragments arranged by size: 1783 86 12 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 916 948 1881 Size: 916 32 933 Fragments arranged by size: 933 916 32 PstI C_TGCA'G Cuts at: 0 484 1881 Size: 484 1397 PvuII CAG'CTG Cuts at: 0 307 600 1881 Size: 307 293 1281 Fragments arranged by size: 1281 307 293 SacI G_AGCT'C Cuts at: 0 350 478 1559 1881 Size: 350 128 1081 322 Fragments arranged by size: 1081 350 322 128 SacII CC_GC'GG Cuts at: 0 28 1881 Size: 28 1853 SanDI GG'GwC_CC Cuts at: 0 300 1881 Size: 300 1581 SapI GCTCTTCn'nnn_ Cuts at: 0 736 1881 Size: 736 1145 ScaI AGT'ACT Cuts at: 0 1471 1881 Size: 1471 410 SexAI A'CCwGG_T Cuts at: 0 569 1881 Size: 569 1312 SfcI C'TryA_G Cuts at: 0 480 1881 Size: 480 1401 SfoI GGC'GCC Cuts at: 0 1524 1881 Size: 1524 357 SmaI CCC'GGG Cuts at: 0 97 1696 1881 Size: 97 1599 185 Fragments arranged by size: 1599 185 97 StyI C'CwwG_G Cuts at: 0 416 1784 1881 Size: 416 1368 97 Fragments arranged by size: 1368 416 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 252 421 1207 1378 1558 1881 Size: 252 169 786 171 180 323 Fragments arranged by size: 786 323 252 180 171 169 TatI w'GTAC_w Cuts at: 0 1469 1715 1881 Size: 1469 246 166 Fragments arranged by size: 1469 246 166 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 890 922 1049 1081 1881 Size: 890 32 127 32 800 Fragments arranged by size: 890 800 127 32 32 Tth111I GACn'n_nGTC Cuts at: 0 181 367 1881 Size: 181 186 1514 Fragments arranged by size: 1514 186 181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 84 567 1128 1333 1881 Size: 84 483 561 205 548 Fragments arranged by size: 561 548 483 205 84 XmaI C'CCGG_G Cuts at: 0 95 1694 1881 Size: 95 1599 187 Fragments arranged by size: 1599 187 95 XmnI GAAnn'nnTTC Cuts at: 0 130 1881 Size: 130 1751 ZraI GAC'GTC Cuts at: 0 492 1881 Size: 492 1389 Enzymes that do cut: AarI AatII AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmgBI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PfoI PpuMI PspOMI PsrI PstI PvuII SacI SacII SanDI SapI ScaI SexAI SfcI SfoI SmaI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BmrI BmtI BplI BpmI BpuEI BsiWI BsmI BspEI BsrGI BstBI BstZ17I Bsu36I ClaI CspCI DraI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MluI NdeI NheI NotI NruI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SalI SbfI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI