Restriction map for
pFN21AE0396
(Linear) (Six-base) MAPSORT of: KIEE0396.seq Check: 5391 from: 1 to: 3120
KIEE0396.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1460 3120
Size: 1460 1660
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 828 1267 1996 2780 2956 2983 3120
Size: 828 439 729 784 176 27 137
Fragments arranged by size:
828 784 729 439 176 137 27
AflIII A'CryG_T
Cuts at: 0 603 1688 3120
Size: 603 1085 1432
Fragments arranged by size:
1432 1085 603
AhdI GACnn_n'nnGTC
Cuts at: 0 342 1274 3120
Size: 342 932 1846
Fragments arranged by size:
1846 932 342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 940 3120
Size: 940 2180
AlwNI CAG_nnn'CTG
Cuts at: 0 933 1215 1494 2430 2709 2754 3120
Size: 933 282 279 936 279 45 366
Fragments arranged by size:
936 933 366 282 279 279 45
ApaI G_GGCC'C
Cuts at: 0 2326 2748 3120
Size: 2326 422 372
Fragments arranged by size:
2326 422 372
ApaLI G'TGCA_C
Cuts at: 0 2576 3120
Size: 2576 544
ApoI r'AATT_y
Cuts at: 0 970 1798 2943 3120
Size: 970 828 1145 177
Fragments arranged by size:
1145 970 828 177
AvaI C'yCGr_G
Cuts at: 0 392 1387 2048 2209 3120
Size: 392 995 661 161 911
Fragments arranged by size:
995 911 661 392 161
AvrII C'CTAG_G
Cuts at: 0 2991 3120
Size: 2991 129
BanI G'GyrC_C
Cuts at: 0 257 1788 2105 2294 2827 3120
Size: 257 1531 317 189 533 293
Fragments arranged by size:
1531 533 317 293 257 189
BanII G_rGCy'C
Cuts at: 0 817 842 1388 2326 2664 2684 2748 3120
Size: 817 25 546 938 338 20 64 372
Fragments arranged by size:
938 817 546 372 338 64 25 20
BbeI G_GCGC'C
Cuts at: 0 261 1792 2109 3120
Size: 261 1531 317 1011
Fragments arranged by size:
1531 1011 317 261
BbsI GAAGACnn'nnnn_
Cuts at: 0 975 1088 1452 1606 3120
Size: 975 113 364 154 1514
Fragments arranged by size:
1514 975 364 154 113
BbvCI CC'TCA_GC
Cuts at: 0 676 1428 3120
Size: 676 752 1692
Fragments arranged by size:
1692 752 676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2477 2511 3120
Size: 2477 34 609
Fragments arranged by size:
2477 609 34
BclI T'GATC_A
Cuts at: 0 2772 3120
Size: 2772 348
BglI GCCn_nnn'nGGC
Cuts at: 0 2032 3120
Size: 2032 1088
BlpI GC'TnA_GC
Cuts at: 0 2591 2794 3120
Size: 2591 203 326
Fragments arranged by size:
2591 326 203
Bme1580I G_kGCm'C
Cuts at: 0 2121 2297 2326 2580 2748 2832 3120
Size: 2121 176 29 254 168 84 288
Fragments arranged by size:
2121 288 254 176 168 84 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 1604 2067 3120
Size: 1604 463 1053
Fragments arranged by size:
1604 1053 463
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 213 3120
Size: 213 2907
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 790 853 1693 2884 2905 3120
Size: 763 27 63 840 1191 21 215
Fragments arranged by size:
1191 840 763 215 63 27 21
Bpu10I CC'TnA_GC
Cuts at: 0 676 1428 3120
Size: 676 752 1692
Fragments arranged by size:
1692 752 676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 2284 2805 3120
Size: 1315 969 521 315
Fragments arranged by size:
1315 969 521 315
BsaI GGTCTCn'nnnn_
Cuts at: 0 141 389 1417 3120
Size: 141 248 1028 1703
Fragments arranged by size:
1703 1028 248 141
BsaAI yAC'GTr
Cuts at: 0 1689 2278 3120
Size: 1689 589 842
Fragments arranged by size:
1689 842 589
BsaBI GATnn'nnATC
Cuts at: 0 1942 3120
Size: 1942 1178
BsaHI Gr'CG_yC
Cuts at: 0 258 1789 2106 3120
Size: 258 1531 317 1014
Fragments arranged by size:
1531 1014 317 258
BsaWI w'CCGG_w
Cuts at: 0 2228 3120
Size: 2228 892
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1498 1528 2143 2173 2651 2681 3120
Size: 1498 30 615 30 478 30 439
Fragments arranged by size:
1498 615 478 439 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 49 126 850 2266 2902 3091 3120
Size: 46 3 77 724 1416 636 189 29
Fragments arranged by size:
1416 724 636 189 77 46 29 3
BseYI C'CCAG_C
Cuts at: 0 365 387 816 1700 2631 3120
Size: 365 22 429 884 931 489
Fragments arranged by size:
931 884 489 429 365 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1420 1627 2159 2258 3120
Size: 919 501 207 532 99 862
Fragments arranged by size:
919 862 532 501 207 99
BsiEI CG_ry'CG
Cuts at: 0 1193 1283 3120
Size: 1193 90 1837
Fragments arranged by size:
1837 1193 90
BsiHKAI G_wGCw'C
Cuts at: 0 88 842 919 1055 2057 2517 2580 3120
Size: 88 754 77 136 1002 460 63 540
Fragments arranged by size:
1002 754 540 460 136 88 77 63
BsmI GAATG_Cn'
Cuts at: 0 1257 3120
Size: 1257 1863
BsmBI CGTCTCn'nnnn_
Cuts at: 0 724 3120
Size: 724 2396
Bsp1286I G_dGCh'C
Cuts at: 0 88 817 842 919 1055 1388 2057 2121
Size: 88 729 25 77 136 333 669 64
Cuts at: 2121 2297 2326 2517 2580 2664 2684 2748 2832
Size: 176 29 191 63 84 20 64 84
Cuts at: 2832 3120
Size: 288
Fragments arranged by size:
729 669 333 288 191 176 136 88
84 84 77 64 64 63 29 25
20
BspEI T'CCGG_A
Cuts at: 0 2228 3120
Size: 2228 892
BspHI T'CATG_A
Cuts at: 0 1380 3120
Size: 1380 1740
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 407 1250 1460 1550 3120
Size: 407 843 210 90 1570
Fragments arranged by size:
1570 843 407 210 90
BsrBI CCG'CTC
Cuts at: 0 474 2256 3120
Size: 474 1782 864
Fragments arranged by size:
1782 864 474
BsrFI r'CCGG_y
Cuts at: 0 410 998 3120
Size: 410 588 2122
Fragments arranged by size:
2122 588 410
BstAPI GCAn_nnn'nTGC
Cuts at: 0 933 1563 1682 1852 2205 2430 3120
Size: 933 630 119 170 353 225 690
Fragments arranged by size:
933 690 630 353 225 170 119
BstEII G'GTnAC_C
Cuts at: 0 1266 3120
Size: 1266 1854
Bsu36I CC'TnA_GG
Cuts at: 0 1300 1366 3120
Size: 1300 66 1754
Fragments arranged by size:
1754 1300 66
BtgI C'CryG_G
Cuts at: 0 563 683 2375 2585 2654 3120
Size: 563 120 1692 210 69 466
Fragments arranged by size:
1692 563 466 210 120 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 294 3120
Size: 294 2826
BtsI GCAGTG_nn'
Cuts at: 0 912 1015 1053 1179 2322 2520 2648 2826
Size: 912 103 38 126 1143 198 128 178
Cuts at: 2826 3120
Size: 294
Fragments arranged by size:
1143 912 294 198 178 128 126 103
38
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1853 1888 3120
Size: 1853 35 1232
Fragments arranged by size:
1853 1232 35
DraIII CAC_nnn'GTG
Cuts at: 0 1852 1940 2182 2333 3120
Size: 1852 88 242 151 787
Fragments arranged by size:
1852 787 242 151 88
DrdI GACnn_nn'nnGTC
Cuts at: 0 687 3120
Size: 687 2433
EaeI y'GGCC_r
Cuts at: 0 251 412 668 855 1016 1280 1919 2672
Size: 251 161 256 187 161 264 639 753
Cuts at: 2672 3120
Size: 448
Fragments arranged by size:
753 639 448 264 256 251 187 161
161
EagI C'GGCC_G
Cuts at: 0 1280 3120
Size: 1280 1840
EarI CTCTTCn'nnn_
Cuts at: 0 2 805 847 1225 1891 2522 3120
Size: 2 803 42 378 666 631 598
Fragments arranged by size:
803 666 631 598 378 42 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1399 1411 3120
Size: 1399 12 1709
Fragments arranged by size:
1709 1399 12
EcoICRI GAG'CTC
Cuts at: 0 840 3120
Size: 840 2280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 790 828 853 1267 1693 1996 2780
Size: 763 27 38 25 414 426 303 784
Cuts at: 2780 2884 2905 2956 2983 3120
Size: 104 21 51 27 137
Fragments arranged by size:
784 763 426 414 303 137 104 51
38 27 27 25 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1231 3010 3120
Size: 1231 1779 110
Fragments arranged by size:
1779 1231 110
EcoO109I rG'GnC_Cy
Cuts at: 0 143 192 369 1514 1606 1668 2061 2094
Size: 143 49 177 1145 92 62 393 33
Cuts at: 2094 2323 2744 2751 3015 3120
Size: 229 421 7 264 105
Fragments arranged by size:
1145 421 393 264 229 177 143 105
92 62 49 33 7
EcoRI G'AATT_C
Cuts at: 0 1798 3120
Size: 1798 1322
HaeII r_GCGC'y
Cuts at: 0 261 1377 1792 2109 3120
Size: 261 1116 415 317 1011
Fragments arranged by size:
1116 1011 415 317 261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 328 360 361 393 1186 1218
Size: 35 32 261 32 1 32 793 32
Cuts at: 1218 1260 1292 1498 1530 2383 2415 3120
Size: 42 32 206 32 853 32 705
Fragments arranged by size:
853 793 705 261 206 42 35 32
32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1127 2306 3084 3120
Size: 1127 1179 778 36
Fragments arranged by size:
1179 1127 778 36
HpaI GTT'AAC
Cuts at: 0 1127 3120
Size: 1127 1993
KasI G'GCGC_C
Cuts at: 0 257 1788 2105 3120
Size: 257 1531 317 1015
Fragments arranged by size:
1531 1015 317 257
MfeI C'AATT_G
Cuts at: 0 1890 2530 3120
Size: 1890 640 590
Fragments arranged by size:
1890 640 590
MscI TGG'CCA
Cuts at: 0 253 670 857 1018 1921 3120
Size: 253 417 187 161 903 1199
Fragments arranged by size:
1199 903 417 253 187 161
MslI CAynn'nnrTG
Cuts at: 0 440 863 2416 3120
Size: 440 423 1553 704
Fragments arranged by size:
1553 704 440 423
MspA1I CmG'CkG
Cuts at: 0 120 930 995 1005 1285 2123 2173 2388
Size: 120 810 65 10 280 838 50 215
Cuts at: 2388 2427 2656 3120
Size: 39 229 464
Fragments arranged by size:
838 810 464 280 229 215 120 65
50 39 10
NaeI GCC'GGC
Cuts at: 0 1000 3120
Size: 1000 2120
NarI GG'CG_CC
Cuts at: 0 258 1789 2106 3120
Size: 258 1531 317 1014
Fragments arranged by size:
1531 1014 317 258
NcoI C'CATG_G
Cuts at: 0 2585 3120
Size: 2585 535
NgoMIV G'CCGG_C
Cuts at: 0 998 3120
Size: 998 2122
NspI r_CATG'y
Cuts at: 0 456 607 656 1573 1680 2203 2606 3120
Size: 456 151 49 917 107 523 403 514
Fragments arranged by size:
917 523 514 456 403 151 107 49
PasI CC'CwG_GG
Cuts at: 0 825 3120
Size: 825 2295
PciI A'CATG_T
Cuts at: 0 603 3120
Size: 603 2517
PflMI CCAn_nnn'nTGG
Cuts at: 0 228 1602 2670 2754 2838 3120
Size: 228 1374 1068 84 84 282
Fragments arranged by size:
1374 1068 282 228 84 84
PfoI T'CCnGG_A
Cuts at: 0 167 767 965 2900 3038 3120
Size: 167 600 198 1935 138 82
Fragments arranged by size:
1935 600 198 167 138 82
PmlI CAC'GTG
Cuts at: 0 2278 3120
Size: 2278 842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 3052 3084 3120
Size: 1138 32 1882 32 36
Fragments arranged by size:
1882 1138 36 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 143 192 369 1514 1606 3120
Size: 143 49 177 1145 92 1514
Fragments arranged by size:
1514 1145 177 143 92 49
PspOMI G'GGCC_C
Cuts at: 0 2322 2744 3120
Size: 2322 422 376
Fragments arranged by size:
2322 422 376
PstI C_TGCA'G
Cuts at: 0 404 929 954 1010 1235 1505 1556 1991
Size: 404 525 25 56 225 270 51 435
Cuts at: 1991 2360 2387 3014 3100 3120
Size: 369 27 627 86 20
Fragments arranged by size:
627 525 435 404 369 270 225 86
56 51 27 25 20
PvuII CAG'CTG
Cuts at: 0 120 995 1005 2173 2388 2427 3120
Size: 120 875 10 1168 215 39 693
Fragments arranged by size:
1168 875 693 215 120 39 10
RsrII CG'GwC_CG
Cuts at: 0 1398 3120
Size: 1398 1722
SacI G_AGCT'C
Cuts at: 0 842 3120
Size: 842 2278
SacII CC_GC'GG
Cuts at: 0 2657 3120
Size: 2657 463
SanDI GG'GwC_CC
Cuts at: 0 143 369 1514 1606 3120
Size: 143 226 1145 92 1514
Fragments arranged by size:
1514 1145 226 143 92
SapI GCTCTTCn'nnn_
Cuts at: 0 805 847 1225 2522 3120
Size: 805 42 378 1297 598
Fragments arranged by size:
1297 805 598 378 42
SbfI CC_TGCA'GG
Cuts at: 0 3014 3120
Size: 3014 106
SfcI C'TryA_G
Cuts at: 0 400 925 950 1006 1231 1501 1529 1552
Size: 400 525 25 56 225 270 28 23
Cuts at: 1552 1987 2356 2383 3010 3096 3120
Size: 435 369 27 627 86 24
Fragments arranged by size:
627 525 435 400 369 270 225 86
56 28 27 25 24 23
SfoI GGC'GCC
Cuts at: 0 259 1790 2107 3120
Size: 259 1531 317 1013
Fragments arranged by size:
1531 1013 317 259
SmaI CCC'GGG
Cuts at: 0 1389 2050 2211 3120
Size: 1389 661 161 909
Fragments arranged by size:
1389 909 661 161
SmlI C'TyrA_G
Cuts at: 0 1330 2299 2784 3120
Size: 1330 969 485 336
Fragments arranged by size:
1330 969 485 336
SphI G_CATG'C
Cuts at: 0 1573 2203 2606 3120
Size: 1573 630 403 514
Fragments arranged by size:
1573 630 514 403
StuI AGG'CCT
Cuts at: 0 1951 2113 3120
Size: 1951 162 1007
Fragments arranged by size:
1951 1007 162
StyI C'CwwG_G
Cuts at: 0 1596 2439 2585 2765 2837 2991 3120
Size: 1596 843 146 180 72 154 129
Fragments arranged by size:
1596 843 180 154 146 129 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 401 883 1207 2395 2501 2722 2758 2815
Size: 401 482 324 1188 106 221 36 57
Cuts at: 2815 3120
Size: 305
Fragments arranged by size:
1188 482 401 324 305 221 106 57
36
TatI w'GTAC_w
Cuts at: 0 1307 3120
Size: 1307 1813
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2143 2175 2651 2683 3120
Size: 2143 32 476 32 437
Fragments arranged by size:
2143 476 437 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 271 1117 1610 3120
Size: 271 846 493 1510
Fragments arranged by size:
1510 846 493 271
XbaI T'CTAG_A
Cuts at: 0 3072 3120
Size: 3072 48
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 828 3120
Size: 261 567 2292
Fragments arranged by size:
2292 567 261
XmaI C'CCGG_G
Cuts at: 0 1387 2048 2209 3120
Size: 1387 661 161 911
Fragments arranged by size:
1387 911 661 161
XmnI GAAnn'nnTTC
Cuts at: 0 1802 3064 3120
Size: 1802 1262 56
Fragments arranged by size:
1802 1262 56
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AlwNI ApaI ApaLI
ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI
BcgI BclI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BstAPI BstEII Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HincII HpaI KasI MfeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII RsrII
SacI SacII SanDI SapI SbfI SfcI SfoI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AloI AscI AseI AsiSI BaeI BamHI BciVI BfrBI
BglII BmgBI BmtI BsiWI BsrDI BsrGI BssHII BssSI
BstBI BstXI BstYI BstZ17I ClaI DraI EcoRV FalI
FseI FspI FspAI HindIII KpnI MluI MmeI NdeI
NheI NotI NruI NsiI PacI PmeI PshAI PsiI
PspXI PsrI PvuI SalI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SspI SwaI XhoI ZraI