Restriction map for pFN21AB8477
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AviII       
AvrII       BamHI       BbeI        BbrPI       BcgI        
BciVI       BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BsiEI       BsiWI       Bsp1407I    BspDI       BspEI       
BsrFI       BsrGI       BssHII      Bst1107I    BstUI       
BstZ17I     CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        DsaI        EagI        
EclXI       Eco47III    Eco52I      EcoRV       FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HpaI        KasI        KpnI        KspI        MaeII       
MluI        MroI        MvnI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SbfI        ScaI        SfiI        SfoI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
StuI        XbaI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

AfaI         2262  GTAC                 GT^AC
AflIII        900  ACATGT               A^C[GA][CT]GT
AhdI         1763  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1995  GTGCAC               G^TGCAC
AocI         1427  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1995  GTGCAC               G^TGCAC
Asp700I      1497  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1763  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          207  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         2551  GGTGCC               G^G[CT][GA]CC
BanII        1398  GAGCTC               G[GA]GC[CT]^C
BbvCI        1629  CCTCAGC              CCTCAGC(-5/-2)
BclI          474  TGATCA               T^GATCA
BglII        1057  AGATCT               A^GATCT
BsaBI         367  GATCGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2454  GAGACC               GGTCTC(1/5)
BsmBI         531  CGTCTC               CGTCTC(1/5)
BsmI           15  GAATGC               GAATGC(1/-1)
BspLU11I      900  ACATGT               A^CATGT
BsrBI        1248  CCGCTC               CCGCTC(-3/-3)
BsrDI         537  CATTGC               GCAATG(2/0)
BssSI         979  CACGAG               CACGAG(-5/-1)
BstAPI        235  GCATCTGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         524  TTCGAA               TT^CGAA
BstEII       2058  GGTTACC              G^GT[ACGT]ACC
BstPI        2058  GGTTACC              G^GT[ACGT]ACC
Bsu36I       1427  CCTCAGG              CC^T[ACGT]AGG
DraII        1422  AGGACCC              [GA]G^G[ACGT]CC[CT]
Eam1105I     1763  GACCAGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1427  CCTCAGG              CC^T[ACGT]AGG
EcoO109I     1422  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I      2058  GGTTACC              G^GT[ACGT]ACC
EcoRI        2130  GAATTC               G^AATTC
EcoT14I       686  CCAAGG               C^C[AT][AT]GG
EcoT22I       233  ATGCAT               ATGCA^T
FauI         1206  GCGGG                CCCGC(4/6)
FbaI          474  TGATCA               T^GATCA
HgaI         1473  GCGTC                GACGC(5/10)
HincII       1475  GTCGAC               GT[CT]^[GA]AC
HindII       1475  GTCGAC               GT[CT]^[GA]AC
HindIII       938  AAGCTT               A^AGCTT
MamI          367  GATCGTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          457  CAATTG               C^AATTG
MunI          457  CAATTG               C^AATTG
NdeI         1094  CATATG               CA^TATG
NsiI          233  ATGCAT               ATGCA^T
NspV          524  TTCGAA               TT^CGAA
PaeR7I       2381  CTCGAG               C^TCGAG
PflFI         207  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        1422  AGGACCC              [GA]G^G[AT]CC[CT]
RsaI         2262  GTAC                 GT^AC
SacI         1398  GAGCTC               GAGCT^C
SalI         1475  GTCGAC               G^TCGAC
SexAI        1764  ACCAGGT              A^CC[AT]GGT
SfuI          524  TTCGAA               TT^CGAA
SmaI         1798  CCCGGG               CCC^GGG
SphI          231  GCATGC               GCATG^C
SspI         2031  AATATT               AAT^ATT
StyI          686  CCAAGG               C^C[AT][AT]GG
SwaI         2618  ATTTAAAT             ATTT^AAAT
Tth111I       207  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2436  CCAGGAGATGTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2381  CTCGAG               C^TCGAG
XmaCI        1798  CCCGGG               C^CCGGG
XmaI         1798  CCCGGG               C^CCGGG
XmnI         1497  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AspHI        1398  GAGCTC               G[AT]GC[AT]^C
             1995  GTGCAC              
AvaI         1798  CCCGGG               C^[CT]CG[GA]G
             2381  CTCGAG              
BalI         2222  TGGCCA               TGG^CCA
             2481  TGGCCA              
BbsI         1334  GTCTTC               GAAGAC(2/6)
             2666  GTCTTC              
BpuAI        1334  GTCTTC               GAAGAC(2/6)
             2666  GTCTTC              
BsgI          976  CTGCAC               GTGCAG(16/14)
             1602  CTGCAC              
BsiHKAI      1398  GAGCTC               G[AT]GC[AT]^C
             1995  GTGCAC              
BsmFI         462  GGGAC                GGGAC(10/14)
             1138  GGGAC               
BsoBI        1798  CCCGGG               C^[CT]CG[GA]G
             2381  CTCGAG              
BspHI          53  TCATGA               T^CATGA
             1307  TCATGA              
BspMI         932  ACCTGC               ACCTGC(4/8)
             1945  GCAGGT              
DraI         1183  TTTAAA               TTT^AAA
             2619  TTTAAA              
EaeI         2222  TGGCCA               [CT]^GGCC[GA]
             2481  TGGCCA              
EcoNI        1362  CCTGAACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2730  CCTGAAGGAGG         
HapII        1799  CCGG                 C^CGG
             2463  CCGG                
HpaII        1799  CCGG                 C^CGG
             2463  CCGG                
MluNI        2222  TGGCCA               TGG^CCA
             2481  TGGCCA              
MscI         2222  TGGCCA               TGG^CCA
             2481  TGGCCA              
MslI          333  CATCGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2121  CACGGCTGTG          
MspI         1799  CCGG                 C^CGG
             2463  CCGG                
NspI          231  GCATGC               [GA]CATG^[CT]
              900  ACATGT              
PflMI        1404  CCAGAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1533  CCAGGTTCTGG         
PstI          490  CTGCAG               CTGCA^G
              622  CTGCAG              
RcaI           53  TCATGA               T^CATGA
             1307  TCATGA              
SapI          844  GAAGAGC              GCTCTTC(1/4)
              676  GCTCTTC             
Van91I       1404  CCAGAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1533  CCAGGTTCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   69 enzymes) #
#########################################

AccI        AciI        AcsI        AluI        AlwI        
AlwNI       ApoI        AvaII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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