################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AviII AvrII BamHI BbeI BbrPI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII Bst1107I BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoRV FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeII MluI MroI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI StuI XbaI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AfaI 2262 GTAC GT^AC AflIII 900 ACATGT A^C[GA][CT]GT AhdI 1763 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1995 GTGCAC G^TGCAC AocI 1427 CCTCAGG CC^T[ACGT]AGG ApaLI 1995 GTGCAC G^TGCAC Asp700I 1497 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1763 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 207 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 2551 GGTGCC G^G[CT][GA]CC BanII 1398 GAGCTC G[GA]GC[CT]^C BbvCI 1629 CCTCAGC CCTCAGC(-5/-2) BclI 474 TGATCA T^GATCA BglII 1057 AGATCT A^GATCT BsaBI 367 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2454 GAGACC GGTCTC(1/5) BsmBI 531 CGTCTC CGTCTC(1/5) BsmI 15 GAATGC GAATGC(1/-1) BspLU11I 900 ACATGT A^CATGT BsrBI 1248 CCGCTC CCGCTC(-3/-3) BsrDI 537 CATTGC GCAATG(2/0) BssSI 979 CACGAG CACGAG(-5/-1) BstAPI 235 GCATCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 524 TTCGAA TT^CGAA BstEII 2058 GGTTACC G^GT[ACGT]ACC BstPI 2058 GGTTACC G^GT[ACGT]ACC Bsu36I 1427 CCTCAGG CC^T[ACGT]AGG DraII 1422 AGGACCC [GA]G^G[ACGT]CC[CT] Eam1105I 1763 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1427 CCTCAGG CC^T[ACGT]AGG EcoO109I 1422 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 2058 GGTTACC G^GT[ACGT]ACC EcoRI 2130 GAATTC G^AATTC EcoT14I 686 CCAAGG C^C[AT][AT]GG EcoT22I 233 ATGCAT ATGCA^T FauI 1206 GCGGG CCCGC(4/6) FbaI 474 TGATCA T^GATCA HgaI 1473 GCGTC GACGC(5/10) HincII 1475 GTCGAC GT[CT]^[GA]AC HindII 1475 GTCGAC GT[CT]^[GA]AC HindIII 938 AAGCTT A^AGCTT MamI 367 GATCGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 457 CAATTG C^AATTG MunI 457 CAATTG C^AATTG NdeI 1094 CATATG CA^TATG NsiI 233 ATGCAT ATGCA^T NspV 524 TTCGAA TT^CGAA PaeR7I 2381 CTCGAG C^TCGAG PflFI 207 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1422 AGGACCC [GA]G^G[AT]CC[CT] RsaI 2262 GTAC GT^AC SacI 1398 GAGCTC GAGCT^C SalI 1475 GTCGAC G^TCGAC SexAI 1764 ACCAGGT A^CC[AT]GGT SfuI 524 TTCGAA TT^CGAA SmaI 1798 CCCGGG CCC^GGG SphI 231 GCATGC GCATG^C SspI 2031 AATATT AAT^ATT StyI 686 CCAAGG C^C[AT][AT]GG SwaI 2618 ATTTAAAT ATTT^AAAT Tth111I 207 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2436 CCAGGAGATGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2381 CTCGAG C^TCGAG XmaCI 1798 CCCGGG C^CCGGG XmaI 1798 CCCGGG C^CCGGG XmnI 1497 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AspHI 1398 GAGCTC G[AT]GC[AT]^C 1995 GTGCAC AvaI 1798 CCCGGG C^[CT]CG[GA]G 2381 CTCGAG BalI 2222 TGGCCA TGG^CCA 2481 TGGCCA BbsI 1334 GTCTTC GAAGAC(2/6) 2666 GTCTTC BpuAI 1334 GTCTTC GAAGAC(2/6) 2666 GTCTTC BsgI 976 CTGCAC GTGCAG(16/14) 1602 CTGCAC BsiHKAI 1398 GAGCTC G[AT]GC[AT]^C 1995 GTGCAC BsmFI 462 GGGAC GGGAC(10/14) 1138 GGGAC BsoBI 1798 CCCGGG C^[CT]CG[GA]G 2381 CTCGAG BspHI 53 TCATGA T^CATGA 1307 TCATGA BspMI 932 ACCTGC ACCTGC(4/8) 1945 GCAGGT DraI 1183 TTTAAA TTT^AAA 2619 TTTAAA EaeI 2222 TGGCCA [CT]^GGCC[GA] 2481 TGGCCA EcoNI 1362 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2730 CCTGAAGGAGG HapII 1799 CCGG C^CGG 2463 CCGG HpaII 1799 CCGG C^CGG 2463 CCGG MluNI 2222 TGGCCA TGG^CCA 2481 TGGCCA MscI 2222 TGGCCA TGG^CCA 2481 TGGCCA MslI 333 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2121 CACGGCTGTG MspI 1799 CCGG C^CGG 2463 CCGG NspI 231 GCATGC [GA]CATG^[CT] 900 ACATGT PflMI 1404 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1533 CCAGGTTCTGG PstI 490 CTGCAG CTGCA^G 622 CTGCAG RcaI 53 TCATGA T^CATGA 1307 TCATGA SapI 844 GAAGAGC GCTCTTC(1/4) 676 GCTCTTC Van91I 1404 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1533 CCAGGTTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccI AciI AcsI AluI AlwI AlwNI ApoI AvaII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII